Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (Western blot analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GPR3 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody. Lane 1: GPR3 transfected lysate (35kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GPR3 monoclonal antibody, Western Blot analysis of GPR3 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (62.04kD).)

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GPR3, Monoclonal Antibody (Cat# AAA25115)

Full Name

GPR3 (G-protein Coupled Receptor 3, ACCA Orphan Receptor, ACCA) (FITC)

Gene Names

GPR3; ACCA

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.Lane 1: FTL transfected lysate(20 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3. GAPDH (36.1 kDa) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])

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FTL, Monoclonal Antibody (Cat# AAA26014)

Full Name

FTL (Ferritin, Light Polypeptide, MGC71996) (AP)

Gene Names

FTL; LFTD; NBIA3

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (HMGB2 monoclonal antibody (M05), clone 3E5 Western Blot analysis of HMGB2 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

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HMGB2, Monoclonal Antibody (Cat# AAA25960)

Full Name

HMGB2 (High-Mobility Group Box 2, HMG2) (AP)

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10. Western Blot analysis of CAND1 expression in Raw 264.7.)

WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10. Western Blot analysis of CAND1 expression in PC-12.)

WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10. Western Blot analysis of CAND1 expression in NIH/3T3.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10 Western Blot analysis of CAND1 expression in Hela S3 NE (Cat # L013V3).)

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CAND1, Monoclonal Antibody (Cat# AAA26499)

Full Name

CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (HRP)

Gene Names

CAND1; TIP120; TIP120A

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)

Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)

FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM7) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM7) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM7) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM7). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

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BCMA, Monoclonal Antibody (Cat# AAA27974)

Full Name

Anti-BCMA antibody (DM7), Rabbit mAb

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$415/0.01 mL $605/0.1 mL (AF350) $605/0.1 mL (AF405L) $605/0.1 mL (AF405M) $605/0.1 mL (AF405S) $605/0.1 mL (AF488) $605/0.1 mL (AF546) $605/0.1 mL (AF555) $605/0.1 mL (AF568) $605/0.1 mL (AF594) $605/0.1 mL (AF610) $605/0.1 mL (AF635) $605/0.1 mL (AF647) $605/0.1 mL (AF680) $605/0.1 mL (AF750) $605/0.1 mL (Biotin) $605/0.1 mL (FITC) $605/0.1 mL (HRP) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (PE) $1,415/2x0.1 mL (AP) $2,050/3x0.1 mL (AP) $2,655/4x0.1 mL (AP)

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)

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AMPKa1/AMPKa2, Monoclonal Antibody (Cat# AAA28599)

Full Name

AMPKa1/AMPKa2 Mouse mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with USP9X antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)

WB (Western Blot) (Western blot analysis of USP9X on different lysates using anti-USP9X antibody at 1/1, 000 dilution. Positive control: Lane 1: SiHa Lane 2: A549 Lane 3: 293 Lane 4: Mouse colon)

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USP9x, Monoclonal Antibody (Cat# AAA30479)

Full Name

USP9x Antibody

Gene Names

USP9X; FAF; FAM; DFFRX; MRX99

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3:ALEXA 647)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3:ALEXA 488)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3:ALEXA 405)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3: RPE- Alexa Fluor® 750)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3: RPE- Alexa Fluor® 647)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3)

Application Data (MCA463F staining of human peripheral blood lymphocytes)

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CD3, Monoclonal Antibody (Cat# AAA26756)

Full Name

CD3 (HRP)

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,115/0.1 mL $2,145/2x0.1 mL $3,115/3x0.1 mL $4,035/4x0.1 mL $4,710/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with Smad2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Smad2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Smad2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Smad2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Smad2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: PC12)

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SMAD2, Monoclonal Antibody (Cat# AAA30017)

Full Name

SMAD2 Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ELISA (Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [8E11]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [8E11]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL.Green: VISTA Antibody [8E11]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

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VISTA, Monoclonal Antibody (Cat# AAA11014)

Full Name

VISTA Antibody [8E11]

Gene Names

VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha

Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12174)

Full Name

RAT ANTI MOUSE F4/80

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot

Pricing

$290/0.025 mg $530/2x0.025 mg $755/3x0.025 mg $970/5x0.025 mg $975/4x0.025 mg

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WB (Western Blot) (Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged RAD51C is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD51C on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody. Lane 1: RAD51C transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RAD51C monoclonal antibody. Western Blot analysis of RAD51C expression in 293.)

WB (Western Blot) (RAD51C monoclonal antibody, Western Blot analysis of RAD51C expression in HeLa.)

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RAD51C, Monoclonal Antibody (Cat# AAA24631)

Full Name

RAD51C (DNA Repair Protein RAD51 Homolog 3, R51H3, RAD51 Homolog C, RAD51-like Protein 2, MGC104277, RAD51L2) APC

Gene Names

RAD51C; FANCO; R51H3; BROVCA3; RAD51L2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of GART transfected lysate using GART monoclonal antibody and Protein A Magnetic Bead and immunoblotted with GART rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GART on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 2ug/ml].)

WB (Western Blot) (Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody. Lane 1: GART transfected lysate (46kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GART monoclonal antibody, Western Blot analysis of GART expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (73.37kD).)

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GART, Monoclonal Antibody (Cat# AAA25108)

Full Name

GART (Trifunctional Purine Biosynthetic Protein Adenosine-3, PGFT, PRGS, MGC47764) (FITC)

Gene Names

GART; AIRS; GARS; PAIS; PGFT; PRGS; GARTF

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged DDX54 is 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody. Lane 1: DDX54 transfected lysate (98.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)

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DDX54, Monoclonal Antibody (Cat# AAA25370)

Full Name

DDX54 (ATP-dependent RNA Helicase DDX54, ATP-dependent RNA Helicase DP97, DEAD Box RNA Helicase 97kD, DEAD Box Protein 54, MGC2835) (HRP)

Gene Names

DDX54; DP97

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])

WB (Western Blot) (Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (87.56kD).)

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PGM1, Monoclonal Antibody (Cat# AAA24901)

Full Name

PGM1 (Phosphoglucomutase-1, Glucose Phosphomutase 1) (Biotin)

Gene Names

PGM1; CDG1T; GSD14

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HMGB1 is approximately 0.3ng/ml as a capture antibody.)

WB (Western Blot) (HMGB1 monoclonal antibody (M08), clone 2F6 Western Blot analysis of HMGB1 expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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HMGB1, Monoclonal Antibody (Cat# AAA26161)

Full Name

HMGB1 (High-Mobility Group Box 1, DKFZp686A04236, HMG1, HMG3, SBP-1) (Biotin)

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged SPP1 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in HepG2.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in COLO 320 HSR.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6 Western Blot analysis of SPP1 expression in Hela S3 NE.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in human stomach.)

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SPP1, Monoclonal Antibody (Cat# AAA25904)

Full Name

SPP1 (Secreted Phosphoprotein 1, BNSP, BSPI, ETA-1, MGC110940, OPN) (AP)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of 293 cells with SUMO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining SUMO4 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining SUMO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining SUMO4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining SUMO4 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot) (Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: Jurkat)

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SUMO4, Monoclonal Antibody (Cat# AAA30248)

Full Name

SUMO4 Antibody

Gene Names

SUMO4; IDDM5; SMT3H4; SUMO-4; dJ281H8.4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (LN18 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD99(ABT156) antibody. The HRP-conjugated Goat anti-M)

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CD99, Monoclonal Antibody (Cat# AAA30879)

Full Name

CD99 (ABT156) Antibody

Gene Names

CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-testis tissue. 1.FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-liver-cancer tissue. 1.FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.FH Monoclonal Antibody(7F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.FH Monoclonal Antibody(7F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.FH Monoclonal Antibody(7F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

WB (Western Blot) (Western blot analysis of 1) 293T. 2) HepG2. 3) Hela. diluted at 1:3000.)

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FH, Monoclonal Antibody (Cat# AAA30812)

Full Name

FH Monoclonal Antibody (7F1)

Gene Names

FH; MCL; LRCC; HLRCC; MCUL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$300/0.04 mL $390/0.1 mL $555/0.2 mL $1,045/2x0.2 mL $1,510/3x0.2 mL $1,955/4x0.2 mL $2,150/5x0.2 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Phospho-eIF-2a (S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Phospho-eIF-2a (S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-eIF-2a (S51) on different lysates using anti-Phospho-eIF-2a (S51) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: PC12)

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eIF-2a, Monoclonal Antibody (Cat# AAA29766)

Full Name

eIF-2a (Phospho-S51) Antibody

Gene Names

EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HARS mAb. [Lot No. HARSA6-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-HARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. HARSA6-2])

IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-HARS mAb. [Lot No. HARSA6-1]Predicted molecular weight: 57 kDa)

WB (Western Blot) (Detection of HARS by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. HARSA6-2]Predicted molecular weight: 57 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-HARS monoclonal antibody.)

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HARS, Monoclonal Antibody (Cat# AAA10601)

Full Name

Mouse monoclonal antibody Anti-Human HARS

Gene Names

HARS; HRS; USH3B

Reactivity

Human

Applications

Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry

Pricing

$615/0.1 mg $1,170/2x0.1 mg $1,695/3x0.1 mg $2,190/4x0.1 mg $2,435/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged SPP1 is ~1ng/ml as a capture antibody.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in NIH/3T3.)

WB (Western Blot) (SPP1 monoclonal antibody, Western Blot analysis of SPP1 expression in Hela NE.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in COLO 320 HSR.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in HeLa.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in human stomach.)

WB (Western Blot) (Western Blot detection against Immunogen (59.11kD).)

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Osteopontin, Monoclonal Antibody (Cat# AAA24594)

Full Name

Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) APC

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of LYPLA3 expression in NIH/3T3)

WB (Western Blot) (Western Blot analysis of PLA2G15 expression in transfected 293T cell line)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in Raw 264.7)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in PC-12)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in HeLa)

WB (Western Blot) (Western Blot detection against Immunogen using (37kD).)

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LYPLA3, Monoclonal Antibody (Cat# AAA25442)

Full Name

LYPLA3 (PLA2G15, Group XV Phospholipase A2, 1-O-acylceramide Synthase, ACS, LCAT-like Lysophospholipase, LLPL, Lysophospholipase 3, Lysosomal Phospholipase A2, LPLA2, UNQ341/PRO540) (HRP)

Gene Names

PLA2G15; ACS; LLPL; LPLA2; LYPLA3; GXVPLA2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])

WB (Western Blot) (TIMP2 monoclonal antibody (M04), clone 5B11 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)

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TIMP2, Monoclonal Antibody (Cat# AAA26282)

Full Name

TIMP2 (TIMP Metallopeptidase Inhibitor 2, CSC-21K) (Biotin)

Gene Names

TIMP2; DDC8; CSC-21K

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M04), clone 5H4.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)

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TESK2, Monoclonal Antibody (Cat# AAA25915)

Full Name

TESK2 (Testis-Specific Kinase 2) (AP)

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E.)

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CD274, Monoclonal Antibody (Cat# AAA26792)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (MaxLight 490)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$830/0.1 mL $1,590/2x0.1 mL $2,305/3x0.1 mL $2,980/4x0.1 mL $3,425/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1.Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GABPA monoclonal antibody.)

Application Data (Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (GABPA monoclonal antibody (M03), clone M1 Western Blot analysis of GABPA expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

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GABPA, Monoclonal Antibody (Cat# AAA26641)

Full Name

GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kD, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A) (PE)

Gene Names

GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

SDS-PAGE (SDS PAGE for Pogalizumab)

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Pogalizumab, Monoclonal Antibody (Cat# AAA18888)

Full Name

Research Grade Pogalizumab

Gene Names

TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L

Reactivity

Human

Purity

>95%; Protein A or G purified from cell culture supernatant.

Pricing

$510/0.1 mg $1,380/1 mg $2,665/2x1 mg $3,875/3x1 mg $5,020/4x1 mg $5,715/5x1 mg

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Application Data (Figure A. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse anti bovine CD335 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated mouse anti bovine CD335 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti bovine CD335 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated mouse anti bovine CD8 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Application Data (Figure A. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse IgG2a isotype control . Figure B. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse anti bovine CD8 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells)

Application Data (Figure A. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse IgG2a isotype control . Figure B. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse anti bovine CD4 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Application Data (Figure A. Alexa Fluor 647 conjugated mouse anti bovine CD4 and FITC conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor 647 conjugated mouse anti bovine CD4 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)

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CD2, Monoclonal Antibody (Cat# AAA12261)

Full Name

Mouse Anti Bovine CD2

Reactivity

Bovine, Goat

Applications

Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$480/0.1 mg $915/2x0.1 mg $1,325/3x0.1 mg $1,705/4x0.1 mg $1,860/5x0.1 mg

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)

Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)

Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)

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CD314, Monoclonal Antibody (Cat# AAA11907)

Full Name

MOUSE ANTI HUMAN CD314:Low Endotoxin

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific Blue)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD45:Alexa Fluor 647)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa Fluor 488)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC)

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CD45, Monoclonal Antibody (Cat# AAA12015)

Full Name

MOUSE ANTI HUMAN CD45

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

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Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488)

Application Data (Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)

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CD59, Monoclonal Antibody (Cat# AAA12022)

Full Name

MOUSE ANTI HUMAN CD59:RPE

Gene Names

CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20

Applications

Flow Cytometry

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TOMM22 monoclonal antibody. Western Blot analysis of TOMM22 expression in PC-12.)

WB (Western Blot) (Western blot analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (41.73kD).)

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TOMM22, Monoclonal Antibody (Cat# AAA24389)

Full Name

TOMM22 (Translocase of Outer Membrane 22kD Subunit Homolog, Mitochondrial Import Receptor Subunit TOM22 Homolog, TOM22, hTom22, 1C9-2, MST065, MSTP065) (AP)

Gene Names

TOMM22; 1C9-2; TOM22; MST065; MSTP065

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)

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ABHD5, Monoclonal Antibody (Cat# AAA24410)

Full Name

ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase Domain-containing Protein 5, Lipid Droplet-binding Protein CGI-58, CGI-58, MGC8731, NCIE2) APC

Gene Names

ABHD5; CDS; CGI58; IECN2; NCIE2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AGTRAP monoclonal antibody, Western Blot analysis of AGTRAP expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged AGTRAP is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AGTRAP on formalin-fixed paraffin-embedded human prostate. [antibody concentration 0.8ug/ml].)

WB (Western Blot) (Western Blot analysis of AGTRAP expression in transfected 293T cell line by AGTRAP monoclonal antibody. Lane 1: AGTRAP transfected lysate (16.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of AGTRAP over-expressed 293 cell line, cotransfected with AGTRAP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with AGTRAP monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot detection against Immunogen (31.83kD).)

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AGTRAP, Monoclonal Antibody (Cat# AAA24423)

Full Name

AGTRAP (ATRAP, Type-1 Angiotensin II Receptor-associated Protein, AT1 Receptor-associated Protein, MGC29646) APC

Gene Names

AGTRAP; ATRAP

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PLK1 is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody. Lane 1: PLK1 transfected lysate (68.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using PLK1 monoclonal antibody.)

WB (Western Blot) (PLK1 monoclonal antibody Western Blot analysis of PLK1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (91.85kD).)

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PLK1, Monoclonal Antibody (Cat# AAA25503)

Full Name

PLK1 (STPK13, Polo-like Kinase 1, Serine/Threonine-protein Kinase 13, Serine/Threonine-protein Kinase PLK1, PLK, PLK-1) (HRP)

Gene Names

PLK1; PLK; STPK13

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

WB (Western Blot) (CPSF6 monoclonal antibody Western Blot analysis of CPSF6 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged CPSF6 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CPSF6 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of CPSF6 expression in transfected 293T cell line by CPSF6 monoclonal antibody. Lane 1: CPSF6 transfected lysate (59.21kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CPSF6 monoclonal antibody Western Blot analysis of CPSF6 expression in Raw 264.7.)

WB (Western Blot) (CPSF6 monoclonal antibody. Western Blot analysis of CPSF6 expression in HL-60.)

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CPSF6, Monoclonal Antibody (Cat# AAA25652)

Full Name

CPSF6 (CFIM68, Cleavage and Polyadenylation Specificity Factor Subunit 6, Cleavage and Polyadenylation Specificity Factor 68kD Subunit, Pre-mRNA Cleavage Factor Im 68kD Subunit, Protein HPBRII-4/7) (PE)

Gene Names

CPSF6; CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CSTF3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (37.07kD).)

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CSTF3, Monoclonal Antibody (Cat# AAA25362)

Full Name

CSTF3 (Cleavage Stimulation Factor Subunit 3, CF-1 77kD Subunit, Cleavage Stimulation Factor 77kD Subunit, CSTF 77kD Subunit, CstF-77) (HRP)

Gene Names

CSTF3; CSTF-77

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)

WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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HNF4A, Monoclonal Antibody (Cat# AAA25420)

Full Name

HNF4A (Hepatocyte Nuclear Factor 4-alpha, HNF-4-alpha, Transcription Factor HNF-4, Nuclear Receptor Subfamily 2 Group A Member 1, Transcription Factor 14, HNF4, NR2A1, TCF14) (HRP)

Gene Names

HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MKRN2 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody. Lane 1: MKRN2 transfected lysate (46.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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MKRN2, Monoclonal Antibody (Cat# AAA25754)

Full Name

MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070) (PE)

Gene Names

MKRN2; RNF62; HSPC070

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged STIP1 is 0.03 ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (STIP1 monoclonal antibody (M06), clone 1C6. Western Blot analysis of STIP1 expression in NIH/3T3.)

WB (Western Blot) (STIP1 monoclonal antibody (M06), clone 1C6. Western Blot analysis of STIP1 expression in PC-12.)

WB (Western Blot) (STIP1 monoclonal antibody (M06), clone 1C6. Western Blot analysis of STIP1 expression in Raw 264.7.)

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STIP1, Monoclonal Antibody (Cat# AAA26679)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (PE)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562)

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ERK1, Monoclonal Antibody (Cat# AAA30019)

Full Name

ERK1 Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (Western BlotPositive WB detected in: 20ug Hela whole cell lysateACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate at 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ugAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysateAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: 293T whole cell lysate, Zebrafish tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time?5min)

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ACTB, Monoclonal Antibody (Cat# AAA28072)

Full Name

ACTB Monoclonal Antibody

Reactivity

Human

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$230/0.05 mL $300/0.1 mL $555/2x0.1 mL $790/3x0.1 mL $1,010/4x0.1 mL $1,190/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:3000 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:3000 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:3000 IF 1:200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:3000 IF 1:200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:3000 IF 1:200 IHC 1:50-300)

WB (Western Blot) (Dilution: WB: 1:3000 IF 1:200 IHC 1:50-300)

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FH, Monoclonal Antibody (Cat# AAA28874)

Full Name

FH Monoclonal Antibody (7F1)

Gene Names

FH; MCL; LRCC; HLRCC; MCUL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 5/6 (ABT-CK5/052) antibody. The HRP-conjugated Goat anti-M)

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Cytokeratin 5/6, Monoclonal Antibody (Cat# AAA30844)

Full Name

Cytokeratin 5/6 (ABT-CK5/052) Antibody

Gene Names

KRT5; K5; CK5; DDD; EBS2; KRT5A

Reactivity

Human

Applications

Immunohistochemistry

Purity

The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of PANC-1 cells with AP2M1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining AP2M1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining AP2M1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining AP2M1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AP2M1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2M1 antibody. Counter stained with hematoxylin.)

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AP2M1, Monoclonal Antibody (Cat# AAA30196)

Full Name

AP2M1 Antibody

Gene Names

AP2M1; mu2; AP50; CLAPM1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with RHEB antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining RHEB in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining RHEB in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RHEB antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RHEB antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RHEB antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of RHEB on mouse placenta tissue lysate using anti-RHEB antibody at 1/1, 000 dilution.)

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RHEB, Monoclonal Antibody (Cat# AAA30496)

Full Name

RHEB Antibody

Gene Names

RHEB; RHEB2

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating GFP in 293F whole cell lysate transfected with GFPLane 1: Mouse control IgG2b instead of AAA28064 in 293F whole cell lysate transfected with GFPLane 2: AAA28064 (4ug) + 293F whole cell lysate transfected with GFP (500ug)Lane 3: 293F whole cell lysate transfected with GFP (5ug)For western blotting, the blot was detected with AAA28064 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)

FCM (Flow Cytometry) (Overlay histogram showing 293F cells transfected with GFP stained with AAA28064 (red line) at 1:200. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

FCM (Flow Cytometry) (Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with AAA28064 at 1:200. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C.)

IF (Immunofluorescence) (Immunofluorescence staining of 293F cells transfected with GFP with AAA28064 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).)

WB (Western Blot) (Western BlotPositive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50ng; 5, 293F whole cell lysate transfected with GFP for 5ugAll lanes GFP antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectivelyObserved band size: 32,32,50,32,32 KDaExposure time?1min)

WB (Western Blot) (Western BlotPositive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ngAll lanes: GFP antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: 50ng recombinant proteinAll lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)

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GFP, Monoclonal Antibody (Cat# AAA28064)

Full Name

GFP Monoclonal Antibody

Reactivity

All

Applications

Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation

Purity

>95%,Protein G purified

Pricing

$230/0.05 mg $300/0.1 mg $555/2x0.1 mg $790/3x0.1 mg $1,010/4x0.1 mg $1,190/5x0.1 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 488)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:APC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:FITC)

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CD33, Monoclonal Antibody (Cat# AAA11929)

Full Name

MOUSE ANTI HUMAN CD33

Gene Names

CD33; p67; SIGLEC3; SIGLEC-3

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.