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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution. Positive control: </br>Lane1: Hela </br>Lane2: HepG2)
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 (alpha+beta) (Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 (alpha+beta) (Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 (alpha+beta) (Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3 (alpha+beta) (Y216+Y279) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3 (alpha+beta) (Y216+Y279) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3 (alpha+beta) (Y216+Y279) antibody. Counter stained with hematoxylin.)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p120 catenin (ABT-p120) antibody. The HRP-conjugated)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Invasive ductal breast carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, TRIS-EDTA of pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast Antibody was diluted at 1:200(4 degree overnight).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, TRIS-EDTA of pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistchemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immmunohistochemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human colon carcinoma tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human colon carcinoma tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
ICC (Immunocytochemistry) (ICC staining KHSRP in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining KHSRP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining KHSRP in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KHSRP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KHSRP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-KHSRP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KHSRP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-KHSRP antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of KHSRP on different lysates using anti-KHSRP antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: mouse brain Lane 3: rat brain)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with PKC epsilon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1, 000 dilution. Positive control�� Lane1: Jurkat Lane2: MCF-7 Lane3: HT-29)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat ovary using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using SUPT5H/SPT5 Rabbit mAb (AAA28496) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Insulin degrading enzyme on hybrid fish (crucian-carp) heart tissue lysate using anti-Insulin degrading enzyme antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of Insulin degrading enzyme on different lysates using anti-Insulin degrading enzyme antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: K562)
WB (Western Blot) (Western blot analysis of COX1 on different lysates using anti-COX1 antibody at 1/1, 000 dilution. Positive control: Lane 1: C2C12 Lane 2: A431)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-COX1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-COX1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-COX1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-COX1 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining COX1 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining COX1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining COX1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining COX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with COX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1, 000 dilution. Positive control�� Lane 1: Rat testis tissue Lane 2: Human placenta tissue Lane 3: Mouse testis tissue Lane 4: 293)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with Exportin-5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with HP1 gamma antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1, 000 dilution.)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4)
Application Data (Detection limit for recombinant GST tagged PAK1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody. Lane 1: PAK1 transfected lysate (Predicted MW: 49.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PAK1 monoclonal antibody Western Blot analysis of PAK1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (KIF2C monoclonal antibody Western Blot analysis of KIF2C expression in HeLa NE.)
WB (Western Blot) (Western Blot analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody Lane 1: KIF2C transfected lysate (81.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01). GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged KIF2C is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of KIF2C transfected lysate using KIF2C monoclonal antibody and Protein A Magnetic Bead and immunoblotted with KIF2C rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KIF2C on HeLa cell. [antibody concentration 10ug/ml].)
Application Data (Immunoperoxidase of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. [antibody concentration 3ug/ml].)
Application Data (Detection limit for recombinant GST tagged IFI16 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IFI16 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to IFI16 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of IFI16 expression in transfected 293T cell line by IFI16 monoclonal antibody. Lane 1: IFI16 transfected lysate (82kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (IFI16 monoclonal antibody Western Blot analysis of IFI16 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IF (Immunofluorescence) (Immunofluorescence to CDKN1B on HeLa cell using 124808 (10ug/ml).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and CDKN1B HeLa cells were stained with AKT1 rabbit purified polyclonal (1:1200) and 124808 (1:50). Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
IHC (Immunohistochemistry) (Immunoperoxidase to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue using 124808 (5ug/ml).)
WB (Western Blot) (Western Blot analysis of CDKN1B expression in transfected 293T cell line by 124808 Lane 1: CDKN1B transfected lysate (22.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (47.89kD).)
Application Data (Detection limit for 124808 is 10ng/ml as a capture antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with ASK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining ASK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ASK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ASK1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data (ATH mouse bone marrow stained with anti-CD44 (clone: KM81) (filled histogram) or Rat IgG2a isotype control (open histogram).N.B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.)
SDS-PAGE (SDS-PAGE Analysis Purified HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1). Confirmation of Purity and Integrity of Antibody.)
WB (Western Blot) (Western Blot Analysis of HEP-2 and PC3 cell lysates using HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)
FCM (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed HeLa cells labeling HSP60 with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red))
IF (Immunofluorescence) (Immunofluorescence Analysis of human MCF-7 cells labeling GPI with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in LNCaP.)
Application Data (Detection limit for recombinant GST tagged MAN1B1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAN1B1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in A-431.)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody Lane 1: HDAC1 transfected lysate (55.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in NIH/3T3)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Raw 264.7)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Hela NE)
WB (Western Blot) (HDAC1 monoclonal antibody. Western Blot analysis of HDAC1 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (78.76kD).)
WB (Western Blot) (NEK10 monoclonal antibody, Western Blot analysis of NEK10 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged NEK10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody. Lane 1: NEK10 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in NIH/3T3.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in Raw 264.7.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M04), clone 5B11 Western Blot analysis of TIMP2 expression in HeLa.)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Daudi Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: 293T Whole Cell Lysate Lane F: HelaS3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: Hela Whole Cell Lysate Lane E: RAW264.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Mouse heart tissue lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse lung tissue lysate Lane E: Mouse kidney tissue lysate Lane F: Mouse brain tissue lysate Lane G: Mouse muscle tissue lysate Lane H: Mouse stomach tissue lysate Lane I: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Rat heart tissue lysate Lane B: Rat liver tissue lysate Lane C: Rat spleen tissue lysate Lane D: Rat lung tissue lysate Lane E: Rat kidney tissue lysate Lane F: Rat muscle tissue lysate Lane G: Rat stomach tissue lysate Lane H: Rat small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Rabbit heart tissue lysate Lane B: Rabbit liver tissue lysate Lane C: Rabbit spleen tissue lysate Lane D: Rabbit lung tissue lysate Lane E: Rabbit kidney tissue lysate Lane F: Rabbit brain tissue lysate Lane G: Rabbit muscle tissue lysate Lane H: Rabbit stomach tissue lysate Lane I: Rabbit small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: Raw246.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.)
IHC (Immunohistochemistry) (Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry) (Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry) (Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-Annexin VI antibody (AAA19709).Overlay histogram showing U87 cells stained with AAA19709 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Annexin VI Antibody (AAA19709, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 7. IF analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Annexin VI was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Annexin VI Antibody (AAA19709) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Annexin VI was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Annexin VI Antibody (AAA19709) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 5. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Annexin VI was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Annexin VI Antibody (AAA19709) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Annexin VI was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Annexin VI Antibody (AAA19709) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 3. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Annexin VI was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Annexin VI Antibody (AAA19709) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 2. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Annexin VI was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Annexin VI Antibody (AAA19709) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of Annexin VI using anti-Annexin VI antibody (AAA19709).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human plancenta tissue lysates,Lane 2: human U87 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat thymus tissue lysates,Lane 6: rat pancreas tissue lysates,Lane 7: mouse thymus tissue lysates,Lane 8: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Annexin VI antigen affinity purified monoclonal antibody (#AAA19709) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Annexin VI at approximately 72 kDa. The expected band size for Annexin VI is at 72 kDa.)
Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. High power)
Application Data (Rat splenocytes stained with Mouse anti Rat CD8 Beta:FITC)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Medium power)
WB (Western Blot) (Figure 11. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat lung tissue lysates,Lane 4: mouse heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse lung tissue lysates,Lane 7: rat RH35 whole cell lysates,Lane 8: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19717) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)
FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of U937 cells using anti-Cyclophilin E/PPIE antibody (AAA19717).Overlay histogram showing U937 cells stained with AAA19717 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin E/PPIE Antibody (AAA19717, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 9. IF analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemistry) (Figure 8. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 7. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 5. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19717) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)
WB (Western Blot) (Detection of human CD79B by western blot. Samples: Whole cell lysate (50 ug) from Daudi, HeLa, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of mouse CD79B by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CH27, CT26, WEHI-231, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human CD79B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used for IP at 20 ul/mg lysate. CD79B was also immunoprecipitated by a second antibody against a different epitope of CD79B (Antibody 2). For blotting immunoprecipitated CD79B, AAA23876 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistchemistry) (Detection of mouse CD79B by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human CD79B by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of mouse CD79B by immunocytochemistry. Sample: FFPE section of CH27 cells. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human CD79B by immunocytochemistry. Sample: FFPE section of daudi cells. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human CD79B (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse CD79B (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with NCK1 rabbit purified polyclonal 1:1200 and ABL2 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged ABL2 is ~0.03ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody. Lane 1: ABL2 transfected lysate (126.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABL2 monoclonal antibody Western Blot analysis of ABL2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (ACSL5 monoclonal antibody, Western Blot analysis of ACSL5 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged ACSL5 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ACSL5 transfected lysate using ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody. Lane 1: ACSL5 transfected lysate (82.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.67kD).)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TOMM22 monoclonal antibody. Western Blot analysis of TOMM22 expression in PC-12.)
WB (Western Blot) (Western blot analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (41.73kD).)
WB (Western Blot) (AKAP10 monoclonal antibody Western Blot analysis of AKAP10 expression in Raw 264.7.)
WB (Western Blot) (RNAi Knockdown: Western blot analysis of AKAP10-over-expressing 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with Cat. # 123131. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged AKAP10 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase staining of AKAP10 on formalin-fixed paraffin-embedded human testis using Cat. # 123131 at 0.5ug/ml.)
WB (Western Blot) (Western Blot analysis of AKAP10 expression in transfected 293T cell line using Cat. # 123131: Lane 1: AKAP10 transfected lysate (73.8kD); Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
AKAP10 (A-kinase Anchor Protein 10, Mitochondrial, AKAP-10, Dual Specificity A Kinase-anchoring Protein 2, D-AKAP-2, Protein Kinase A-anchoring Protein 10, PRKA10) (PE)
Application Data (Detection limit for recombinant GST tagged HIP1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HIP1 transfected lysate using HIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIP1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody Lane 1: HIP1 transfected lysate (116.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HIP1 monoclonal antibody Western Blot analysis of HIP1 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (Western Blot detection against Immunogen (84.74kD).)
WB (Western Blot) (PHGDH monoclonal antibody. Western Blot analysis of PHGDH expression in human liver.)
IP (Immunoprecipitation) (Immunoprecipitation of PHGDH transfected lysate using PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1-10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1-10ug/ml])
WB (Western Blot) (Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody. Lane 1: PHGDH transfected lysate (56.7kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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