Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Detection limit for recombinant GST tagged PCNA is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PCNA transfected lysate using PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody Lane 1: PCNA transfected lysate (28.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PCNA monoclonal antibody Western Blot analysis of PCNA expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (54.45kD).)

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PCNA, Monoclonal Antibody (Cat# AAA25193)

Full Name

PCNA (Proliferating Cell Nuclear Antigen, Cyclin, MGC8367) (FITC)

Gene Names

PCNA; ATLD2

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

WB (Western Blot) (POLK monoclonal antibody, Western Blot analysis of POLK expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged POLK is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody. Lane 1: POLK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

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POLK, Monoclonal Antibody (Cat# AAA25212)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (FITC)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ILK over-expressed 293 cell line, cotransfected with ILK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ILK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ILK is ~3ng/ml as a capture antibody.)

WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in HepG2.)

WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in PC-12.)

WB (Western Blot) (ILK monoclonal antibody Western Blot analysis of ILK expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (38.06kD).)

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ILK, Monoclonal Antibody (Cat# AAA25723)

Full Name

ILK (Integrin-linked Protein Kinase, 59kD Serine/Threonine-protein Kinase, ILK-1, ILK-2, p59ILK, ILK1, ILK2, DKFZp686F1765) (PE)

Gene Names

ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TGIF2 monoclonal antibody, Western Blot analysis of TGIF2 expression in HeLa NE.)

WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in SW-13.)

WB (Western Blot) (Western blot analysis of TGIF2 over-expressed 293 cell line, cotransfected with TGIF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TGIF2 monoclonal antibody (M01). GAPDH (36.1kD). used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TGIF2 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of TGIF2 expression in transfected 293T cell line by TGIF2 monoclonal antibody. Lane 1: TGIF2 transfected lysate (25.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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TGIF2, Monoclonal Antibody (Cat# AAA25864)

Full Name

TGIF2 (TGF-beta-induced Transcription Factor 2, TGFB-induced Factor 2, Homeobox Protein TGIF2, 5'-TG-3'-interacting Factor 2) (PE)

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged RUNX2 is approximately 10ng/ml as a capture antibody.)

WB (Western Blot) (RUNX2 monoclonal antibody (M04), clone 4D5 Western Blot analysis of RUNX2 expression in PC-12 (Cat # L012V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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RUNX2, Monoclonal Antibody (Cat# AAA25978)

Full Name

RUNX2 (Runt-Related Transcription Factor 2, AML3, CBFA1, CCD, CCD1, MGC120022, MGC120023, OSF2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1) (AP)

Gene Names

RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate(105.4 KDa).Lane 2: Non-transfected lysate.)

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NFKB1, Monoclonal Antibody (Cat# AAA25961)

Full Name

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (AP)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cytokeratin 18 on different lysates using anti-Cytokeratin 18 antibody at 1/20, 000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse colon Lane 3: Mouse kidney)

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Cytokeratin 18, Monoclonal Antibody (Cat# AAA30013)

Full Name

Cytokeratin 18 Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

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WB (Western Blot) (Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000)

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Caspase 9, Monoclonal Antibody (Cat# AAA30814)

Full Name

Caspase 9 Monoclonal Antibody (3-20)

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$300/0.04 mL $390/0.1 mL $555/0.2 mL $1,045/2x0.2 mL $1,510/3x0.2 mL $1,955/4x0.2 mL $2,150/5x0.2 mL

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ICC (Immunocytochemistry) (ICC staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of THY1 on different lysates using anti-THY1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC)

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THY1, Monoclonal Antibody (Cat# AAA30295)

Full Name

THY1 Antibody

Gene Names

THY1; CD90

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining IRF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining IRF7 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IRF7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRF7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IRF7 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of IRF7 on hybrid fish (crucian-carp) brain tissue lysate using anti-IRF7 antibody at 1/500 dilution.)

WB (Western Blot) (Western blot analysis of IRF7 on different lysates using anti-IRF7 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Raji)

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IRF7, Monoclonal Antibody (Cat# AAA30150)

Full Name

IRF7 Antibody

Gene Names

IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Wilms' tumor using WT1, Wilms' Tumor 1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Wilms' tumor using WT1, Wilms' Tumor 1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Ovarian serous cystadenocarcinoma using WT1, Wilms' Tumor 1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Mesothelioma using WT1, Wilms' Tumor 1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Mesothelioma using WT1, Wilms' Tumor 1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Mesothelioma using WT1, Wilms' Tumor 1 antibody.)

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Wilms' Tumor 1 (WT1), Monoclonal Antibody (Cat# AAA30741)

Full Name

Anti-Wilms' Tumor 1 (WT1) Antibody [ABT-WT1]

Gene Names

WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2

Reactivity

Human

Applications

Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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Application Data

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IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil using C2399-07G2, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of an acetone fixed, frozen human colon section using C2399-07G2.)

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CD45, Monoclonal Antibody (Cat# AAA26733)

Full Name

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (Biotin)

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Equine, Human

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation

Purity

Purified by Protein A Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:RPE (MCA2872PE))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 488 (MCA2872A488))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 647 (MCA2872A647))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:FITC (MCA2872F)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872GA))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872T))

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CD49d, Monoclonal Antibody (Cat# AAA26760)

Full Name

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (HRP)

Reactivity

Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Phospho-Smad5 (S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Smad5 (S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-Smad5 (S463/465) on different lysates using anti-Phospho-Smad5 (S463/465) antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain)

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SMAD5, Monoclonal Antibody (Cat# AAA29770)

Full Name

SMAD5 (Phospho-S463/465) Antibody

Gene Names

SMAD5; DWFC; JV5-1; MADH5

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)

Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)

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CD3, Monoclonal Antibody (Cat# AAA12156)

Full Name

MOUSE ANTI HUMAN CD3

Gene Names

CD3G; T3G; IMD17; CD3-GAMMA

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$275/0.025 mg $505/2x0.025 mg $725/3x0.025 mg $920/5x0.025 mg $935/4x0.025 mg

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12163)

Full Name

RAT ANTI MOUSE F4/80:Biotin

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow Cytometry

Pricing

$830/0.5 mg $1,590/2x0.5 mg $2,305/3x0.5 mg $2,980/4x0.5 mg $3,415/5x0.5 mg

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Application Data

Application Data

Application Data (Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)

IP (Immunoprecipitation) (Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

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AHR, Monoclonal Antibody (Cat# AAA24130)

Full Name

AHR (Aryl Hydrocarbon Receptor, Ah Receptor, AhR, Class E Basic Helix-loop-helix Protein 76, bHLHe76, BHLHE76) (AP)

Gene Names

AHR; bHLHe76

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)

Application Data (Detection limit for recombinant GST tagged RAB7B is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RAB7B transfected lysate using RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody. Lane 1: RAB7B transfected lysate (22.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in human kidney.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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RAB7B, Monoclonal Antibody (Cat# AAA25221)

Full Name

RAB7B (Ras-related Protein Rab-7b, RAB7) (FITC)

Gene Names

RAB7B; RAB7

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CSNK2A1 monoclonal antibody, Western Blot analysis of CSNK2A1 expression in Hela NE.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and CSNK2A1 HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and CSNK2A1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged CSNK2A1 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSNK2A1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody. Lane 1: CSNK2A1 transfected lysate (45.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

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CSNK2A1, Monoclonal Antibody (Cat# AAA25361)

Full Name

CSNK2A1 (Casein Kinase II Subunit alpha, CK II alpha, CK2A1) (HRP)

Gene Names

CSNK2A1; CKII; CK2A1; OCNDS; CSNK2A3

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MPP1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.)

Application Data (Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.)

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MPP1, Monoclonal Antibody (Cat# AAA25946)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (AP)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431.)

WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26223)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (Biotin)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GSR is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GSR on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)

WB (Western Blot) (Western Blot analysis of GSR expression in transfected 293T cell line by GSR monoclonal antibody. Lane 1: GSR transfected lysate (56.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GSR monoclonal antibody Western Blot analysis of GSR expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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GSR, Monoclonal Antibody (Cat# AAA25707)

Full Name

GSR (Glutathione Reductase, Mitochondrial, GR, GRase, GSR, GLUR, GRD1, MGC78522) (PE)

Gene Names

GSR; HEL-75; HEL-S-122m

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.Lane 1: FTL transfected lysate (20 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3. GAPDH (36.1 kDa) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562 (Cat # L009V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])

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FTL, Monoclonal Antibody (Cat# AAA26546)

Full Name

FTL (Ferritin, Light Polypeptide, MGC71996) (HRP)

Gene Names

FTL; LFTD; NBIA3

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b used under the same conditions. Acquisition of >10,000 events was performed.)

IHC (Immunohistochemistry) (IHC image diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.)

IHC (Immunohistochemistry) (IHC image diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot) (Western Blot Positive WB detected in: 20ug Hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s)

WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug All lanes: HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s)

WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s)

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HSPA8, Monoclonal Antibody (Cat# AAA27045)

Full Name

HSPA8 Monoclonal Antibody

Gene Names

HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Purity

>95%, Protein A purified

Pricing

$305/0.05 mL $400/0.1 mL $750/2x0.1 mL $1,090/3x0.1 mL $1,400/4x0.1 mL $1,705/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Aldolase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Aldolase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Aldolase on Zebrafish tissue lysates using anti-Aldolase antibody at 1/200 dilution.)

WB (Western Blot) (Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: Mouse liver Lane 4: Rat liver Lane 5: Rat spleen)

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Aldolase, Monoclonal Antibody (Cat# AAA30386)

Full Name

Aldolase Antibody

Gene Names

ALDOA; ALDA; GSD12

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Villin1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Villin1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Villin1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse colon Lane 2: Human small intestine Lane 3: Human colon Lane 4: Rat kidney)

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Villin1, Monoclonal Antibody (Cat# AAA30420)

Full Name

Villin1 Antibody

Gene Names

VIL1; VIL; D2S1471

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MMP13 on MCF-7 cells lysates using anti-MMP13 antibody at 1/1, 000 dilution.)

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MMP13, Monoclonal Antibody (Cat# AAA30267)

Full Name

MMP13 Antibody

Gene Names

MMP13; CLG3; MANDP1

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.)

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NEFM, Monoclonal Antibody (Cat# AAA30325)

Full Name

NEFM Antibody

Gene Names

NEFM; NFM; NEF3; NF-M

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Ring1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Ring1 on different cell lysate using anti-Ring1 antibody at 1/1, 000 dilution. Positive control�� Line1: MOLT-4 Line2: LNCaP Line3: Hela Line4: HEK-293 Line5: Jurkat)

WB (Western Blot) (Western blot analysis of Ring1 on HEK293 (1) and Ring1-hIgGFc transfected HEK293 (2) cell lysate using anti-Ring1 antibody at 1/1, 000 dilution.)

WB (Western Blot) (Western blot analysis of Ring1 on human Ring1 recombinant protein using anti-Ring1 antibody at 1/1, 000 dilution.)

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RING1, Monoclonal Antibody (Cat# AAA29906)

Full Name

RING1 Antibody

Gene Names

RING1; RNF1; RING1A

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Androgen receptor in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.)

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Androgen, Monoclonal Antibody (Cat# AAA30084)

Full Name

Androgen Receptor Antibody

Gene Names

AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ELISA (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with abs at decreasing concentrations.)

IHC (Immunohistochemistry) ((A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.)

FCM (Flow Cytometry) (Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [10B3] antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)

IF (Immunofluorescence) (Immunofluorescence of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL.Green: PD1 Antibody [10B3]Blue: DAPI staining)

IHC (Immunohistochemistry) ((A) Immunohistochemistry of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human spleen tissue with control mouse IgG staining at 10 μg/mL.)

ICC (Immunocytochemistry) (Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [10B3] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)

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PD-1, Monoclonal Antibody (Cat# AAA10981)

Full Name

PD-1 Antibody [10B3]

Gene Names

PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified IgG1.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody. Lane 1: PIP5K3 transfected lysate (50.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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PIP5K3, Monoclonal Antibody (Cat# AAA24908)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PCDH8 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody. Lane 1: PCDH8 transfected lysate (113kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PCDH8 monoclonal antibody Western Blot analysis of PCDH8 expression in COLO 320 HSR.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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PCDH8, Monoclonal Antibody (Cat# AAA24599)

Full Name

PCDH8 (Protocadherin-8, Arcadlin) APC

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMURF1 monoclonal antibody, Western Blot analysis of SMURF1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)

Application Data (Detection limit for recombinant GST tagged SMURF1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of SMURF1 transfected lysate using SMURF1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SMURF1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2ug/ml].)

WB (Western Blot) (Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody. Lane 1: SMURF1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)

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SMURF1, Monoclonal Antibody (Cat# AAA24367)

Full Name

SMURF1 (E3 Ubiquitin-protein Ligase SMURF1, hSMURF1, SMAD Ubiquitination Regulatory Factor 1, KIAA1625, SMAD-specific E3 Ubiquitin-protein Ligase 1) (AP)

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PCMT1 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (PCMT1 monoclonal antibody. Western Blot analysis of PCMT1 expression in NIH/3T3.)

WB (Western Blot) (PCMT1 monoclonal antibody. Western Blot analysis of PCMT1 expression in Raw 264.7.)

WB (Western Blot) (PCMT1 monoclonal antibody. Western Blot analysis of PCMT1 expression in PC-12.)

WB (Western Blot) (PCMT1 monoclonal antibody Western Blot analysis of PCMT1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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PCMT1, Monoclonal Antibody (Cat# AAA25486)

Full Name

PCMT1 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, Protein-beta-Aspartate Methyltransferase, Protein L-isoaspartyl/D-aspartyl Methyltransferase, L-isoaspartyl Protein Carboxyl Methyltransferase) (HRP)

Gene Names

PCMT1; PIMT

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of ATP6V1G2 expression in transfected 293T cell line by ATP6V1G2 monoclonal antibody. Lane 1: ATP6V1G2 transfected lysate (13.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in NIH/3T3.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in Raw 264.7.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody, Western Blot analysis of ATP6V1G2 expression in HepG2.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody. Western Blot analysis of ATP6V1G2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (34.32kD).)

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ATP6V1G2, Monoclonal Antibody (Cat# AAA25621)

Full Name

ATP6V1G2 (V-type Proton ATPase Subunit G 2, V-ATPase Subunit G 2, V-ATPase 13kD Subunit 2, Vacuolar Proton Pump Subunit G 2, ATP6G, ATP6G2, NG38) (PE)

Gene Names

ATP6V1G2; NG38; ATP6G; VMA10; ATP6G2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IP (Immunoprecipitation) (Immunoprecipitation of CDC25C transfected lysate using CDC25C monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CDC25C rabbit polyclonal antibody.)

Application Data (Detection limit for recombinant GST tagged CDC25C is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)

WB (Western Blot) (Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody. Lane 1: CDC25C transfected lysate (53.312kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CDC25C monoclonal antibody, Western Blot analysis of CDC25C expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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CDC25C, Monoclonal Antibody (Cat# AAA25640)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (PE)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.)

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STIP1, Monoclonal Antibody (Cat# AAA26407)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.)

WB (Western Blot) (GLUD2 monoclonal antibody (M01), clone 3C2. Western Blot analysis of GLUD2 expression in human pancreas.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])

IP (Immunoprecipitation) (Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate (61 KDa).Lane 2: Non-transfected lysate.)

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GLUD2, Monoclonal Antibody (Cat# AAA26273)

Full Name

GLUD2 (Glutamate Dehydrogenase 2, GDH2, GLUDP1) (Biotin)

Gene Names

GLUD2; GDH2; GLUDP1

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562 (Cat # L009V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

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CSE1L, Monoclonal Antibody (Cat# AAA26290)

Full Name

CSE1L (CSE1 Chromosome Segregation 1-like (yeast), CAS, CSE1, MGC117283, MGC130036, MGC130037, XPO2) (FITC)

Gene Names

CSE1L; CAS; CSE1; XPO2

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of AAA27043 in Hela whole cell lysate. Lane 2: AAA27043 (5ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug) For western blotting, the blot was detected with AAA27043 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)

IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

IHC (Immunohistchemistry) (IHC image diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

IHC (Immunohistochemistry) (IHC image diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

IHC (Immunohistochemistry) (IHC image diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot) (Western Blot: Positive WB detected in: 15ug hela whole cell lysate GAPDH antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

WB (Western Blot) (Western Blot: Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ug, 0.15625ug, 0.078ug All lanes:GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

WB (Western Blot) (Western Blot: Positive WB detected in: Rabbit heart tissue, Rabbit kidney tissue, Rabbit skeletal muscle tissueAll lanes GAPDH antibody at 1:5000Secondary: Goat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

WB (Western Blot) (Western Blot: Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat spleen tissue, Rat brain tissue, Rat skeletal tissue, Rat stomach tissue, Rat kidney tissueAll lanes GAPDH antibody at 1:5000Secondary: Goat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

WB (Western Blot) (Western Blot Positive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min)

WB (Western Blot) (Western Blot Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min)

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GAPDH, Monoclonal Antibody (Cat# AAA27043)

Full Name

GAPDH Monoclonal Antibody

Reactivity

Human, Rat, Rabbit, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$230/0.05 mL $300/0.1 mL $555/2x0.1 mL $790/3x0.1 mL $1,010/4x0.1 mL $1,190/5x0.1 mL

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Application Data (Staining of CD209 transfected K562 cells with Mouse anti Human CD209: Biotin)

Application Data (Staining of CD209 transfected K562 cells with Mouse anti Human CD209: FITC)

Application Data (Staining of CD209 transfected K562 cells with Mouse anti Human CD209)

Application Data (Staining of CD209 transfected K562 cells with Mouse anti Human CD209)

Application Data (Staining of CD209 transfected K562 cells with Mouse anti Human CD209: Alexa Fluor 488)

Application Data (Staining of CD209 transfected K562 cells with Mouse anti Human CD209: Alexa Fluor 647)

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CD209, Monoclonal Antibody (Cat# AAA11906)

Full Name

MOUSE ANTI HUMAN CD209:Low Endotoxin

Gene Names

CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

Applications

Flow Cytometry, Functional Assay, Immunofluorescence

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 488)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:APC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:FITC)

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CD33, Monoclonal Antibody (Cat# AAA11861)

Full Name

MOUSE ANTI HUMAN CD33:FITC

Gene Names

CD33; p67; SIGLEC3; SIGLEC-3

Applications

Flow Cytometry

Pricing

$530/0.1 mg $1,005/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,070/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged SHMT1 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody. Lane 1: SHMT1 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SHMT1 monoclonal antibody, Western Blot analysis of SHMT1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

Compare (5 max)

SHMT1, Monoclonal Antibody (Cat# AAA24359)

Full Name

SHMT1 (Serine Hydroxymethyltransferase, Cytosolic, SHMT, Serine Methylase, Glycine Hydroxymethyltransferase, MGC15229, MGC24556) (AP)

Gene Names

SHMT1; SHMT; CSHMT

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RASSF8 monoclonal antibody Western Blot analysis of RASSF8 expression in HeLa.)

WB (Western Blot) (RASSF8 monoclonal antibody Western Blot analysis of RASSF8 expression in PC-12)

IP (Immunoprecipitation) (Immunoprecipitation of RASSF8 transfected lysate using RASSF8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RASSF8 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RASSF8 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RASSF8 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (RASSF8 monoclonal antibody Western Blot analysis of RASSF8 expression in NIH/3T3)

WB (Western Blot) (Western Blot detection against Immunogen (36.52kD).)

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RASSF8, Monoclonal Antibody (Cat# AAA24336)

Full Name

RASSF8 (Ras Association Domain-containing Protein 8, Carcinoma-associated Protein HOJ-1, C12orf2) (AP)

Gene Names

RASSF8; HOJ1; C12orf2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of DHFR expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence on HeLa cells using 125814 (10ug/ml).)

Application Data (Detection limit for 125814 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DHFR expression in transfected 293T cell line using 125814. Lane 1: DHFR transfected lysate (21.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of DHFR expression in PC-12 using 125814.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

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DHFR, Monoclonal Antibody (Cat# AAA25667)

Full Name

DHFR (Dihydrofolate reductase) (PE)

Gene Names

DHFR; DYR; DHFRP1

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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TCF7L2, Monoclonal Antibody (Cat# AAA25955)

Full Name

TCF7L2 (Transcription Factor 7-like 2 (T-Cell Specific, HMG-Box), TCF-4, TCF4) (AP)

Gene Names

TCF7L2; TCF4; TCF-4

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged KLF10 is 1 ng/ml as a capture antibody.)

WB (Western Blot) (KLF10 monoclonal antibody (M20), clone 2H7. Western Blot analysis of KLF10 expression in Raw 264.7.)

WB (Western Blot) (KLF10 monoclonal antibody (M20), clone 2H7. Western Blot analysis of KLF10 expression in NIH/3T3.)

WB (Western Blot) (KLF10 monoclonal antibody (M20), clone 2H7. Western Blot analysis of KLF10 expression in HepG2.)

WB (Western Blot) (KLF10 monoclonal antibody (M20), clone 2H7. Western Blot analysis of KLF10 expression in human colon.)

WB (Western Blot) (KLF10 monoclonal antibody (M20), clone 2H7. Western Blot analysis of KLF10 expression in A-549 (Cat # L025V1).)

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KLF10, Monoclonal Antibody (Cat# AAA26268)

Full Name

KLF10 (Kruppel-like Factor 10, EGRA, TIEG, TIEG1) (Biotin)

Gene Names

KLF10; EGRA; TIEG; TIEG1; EGR-alpha

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)

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HSP70/HSC70, Monoclonal Antibody (Cat# AAA27659)

Full Name

HSP70/HSC70 Antibody

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Bovine, Sheep, Dog, Beluga, Fish, Guinea Pig, Pig, Hamster, Rabbit, Chicken, African Clawed Frog, Fruit Fly, Yeast

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

Protein G Purified

Pricing

$570/0.2 mg $1,080/2x0.2 mg $1,560/3x0.2 mg $2,015/4x0.2 mg $2,170/5x0.2 mg

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in Raw 264.7.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in PC-12.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in NIH/3T3.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in Hela S3 NE (Cat # L013V3).)

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CAND1, Monoclonal Antibody (Cat# AAA26517)

Full Name

CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (HRP)

Gene Names

CAND1; TIP120; TIP120A

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.