Active Proteins

AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.

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Bioactivity (Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant bovine AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant bovine AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant bovine AP is >3000 U/mg.)

SDS-PAGE (Figure. SDS-PAGE)

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Aprotinin (AP), Active Protein (Cat# AAA161811)

Full Name

Active Aprotinin (AP)

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$220/0.01 mg $410/0.05 mg $610/0.1 mg $745/0.2 mg $1,405/0.5 mg $2,080/1 mg $2,745/2 mg $5,050/5 mg $9,845/2x5 mg $14,385/3x5 mg $18,675/4x5 mg $22,865/5x5 mg

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Bioactivity (Tissue Inhibitors of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. The activity of recombinant bovine TIMP4 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rbTIMP4 (MW: 24.05 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rbTIMP4 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant bovine TIMP4 significantly decreased rhMMP2 activity. The inhibition IC50 was )

SDS-PAGE (Figure. SDS-PAGE)

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Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), Active Protein (Cat# AAA161701)

Full Name

Active Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$340/0.01 mg $725/0.05 mg $1,105/0.1 mg $1,365/0.2 mg $2,655/0.5 mg $3,920/1 mg $5,200/2 mg $9,570/5 mg $18,660/2x5 mg $27,265/3x5 mg $35,400/4x5 mg

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Bioactivity (Procollagen II N-Terminal Propeptide is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces, which was are associated wit)

SDS-PAGE (Figure. SDS-PAGE)

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Procollagen II N-Terminal Propeptide (PIINP), Active Protein (Cat# AAA153010)

Full Name

Active Procollagen II N-Terminal Propeptide (PIINP)

Gene Names

COL2A1; AOM; ANFH; SEDC; COL11A3

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$340/0.01 mg $705/0.05 mg $1,090/0.1 mg $1,335/0.2 mg $2,600/0.5 mg $3,845/1 mg $5,110/2 mg $9,390/5 mg $18,305/2x5 mg $26,750/3x5 mg $34,730/4x5 mg

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Application Data

Application Data

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Fractalkine (CX3CL1), Active Protein (Cat# AAA214279)

Full Name

Recombinant Human Fractalkine (CX3CL1)

Gene Names

CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

Purity

>95% as determined by SDS-PAGE

Pricing

$400/0.02 mg $5,190/1 mg $10,120/2x1 mg $14,785/3x1 mg $19,195/4x1 mg $22,965/5x1 mg

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Bioactivity

SDS-PAGE

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Angiotensin-Converting Enzyme 2/ACE-2, Active Protein (Cat# AAA177979)

Full Name

Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-Fc)

Purity

>95% as determined by reducing SDS-PAGE

Pricing

$295/0.01 mg $840/0.05 mg $1,630/2x0.05 mg $2,380/3x0.05 mg $3,085/4x0.05 mg $3,875/5x0.05 mg

Activity (Figure 1 : ACE2 (human)(rec.) (AAA78521) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (AAA78521 ) is coated on an ELISA plate at 1 ug/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) is added (starting at a concentration of 500 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).)

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ACE2, Active Protein (Cat# AAA78521)

Full Name

Human ACE2 Protein (DYKDDDDK Tag)

Applications

Functional Assay

Purity

>95% (SDS-PAGE)

Pricing

$625/0.05 mg $1,210/2x0.05 mg $1,765/3x0.05 mg $2,295/4x0.05 mg $2,600/5x0.05 mg

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Bioactivity (HTRF Assay for Recombinant YES1 activity. 1 uM TK substrate was incubated with different concentrations of YES1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF Assay for Recombinant YES1 activity. 1 uM TK substrate was incubated with different concentrations of YES1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant YES1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 62.1 kDa Purity: >50%)

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YES1, Active Protein (Cat# AAA60336)

Full Name

Recombinant YES1 protein

Purity

>50%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

SDS-PAGE

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SUMO1, Active Protein (Cat# AAA258013)

Full Name

SUMO1 Protein, Human, Recombinant (His Tag)

Gene Names

SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3

Purity

>90% as determined by SDS-PAGE

Pricing

$375/0.05 mg $1,945/0.5 mg $3,785/2x0.5 mg $5,535/3x0.5 mg $7,185/4x0.5 mg $8,540/5x0.5 mg

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Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His is 200-800 ng/mL.)

SDS-PAGE

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COVID 19 Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His Coronavirus, Active Protein (Cat# AAA258080)

Full Name

SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His Recombinant Protein

Purity

>85% as determined by SDS-PAGE

Pricing

$610/0.1 mg $1,180/2x0.1 mg $1,725/3x0.1 mg $2,235/4x0.1 mg $2,530/5x0.1 mg

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Application Data (Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 60-300 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.)

SDS-PAGE

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ALK-1, Active Protein (Cat# AAA258143)

Full Name

ALK-1 Protein, Mouse, Recombinant (His & hFc Tag)

Gene Names

Acvrl1; Alk1; Acvrlk1; AI115505; AI427544

Purity

>95% as determined by SDS-PAGE

Pricing

$610/0.2 mg $1,180/2x0.2 mg $1,725/3x0.2 mg $2,235/4x0.2 mg $2,530/5x0.2 mg

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Bioactivity (Surfactant Associated Protein C (SPC) is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Monokine Induced By Interferon Gamma (MIg) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SPC and recombinant rat MIg. Briefly, SPC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to MIg-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SPC and MIg was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of SPC with MIg.)

Gene Sequencing (Figure. Gene Sequencing (Extract))

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Surfactant Protein C, Active Protein (Cat# AAA150111)

Full Name

Active Surfactant Protein C (SP-C)

Gene Names

Sftpc; SP-C

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

$320/0.01 mg $665/0.05 mg $1,010/0.1 mg $1,250/0.2 mg $2,430/0.5 mg $3,595/1 mg $4,770/2 mg $8,760/5 mg $17,075/2x5 mg $24,955/3x5 mg $32,400/4x5 mg

Application Data

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Platelet Factor 4, Active Protein (Cat# AAA37788)

Full Name

Human Platelet Factor 4

Gene Names

PF4; PF-4; CXCL4; SCYB4

Purity

>95% by SDS-PAGE analysis

Pricing

$795/0.1 mg $1,540/2x0.1 mg $2,240/3x0.1 mg $2,910/4x0.1 mg $3,330/5x0.1 mg

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized fby coomassie blue stain.)

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Peroxiredoxin 6, Active Protein (Cat# AAA48548)

Full Name

Peroxiredoxin 6, 1-224aa, Human, His tag, E Coli (Bioactivity Validated)

Gene Names

PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m

Applications

SDS-PAGE, Enzyme Activity

Purity

> 95% by SDS-PAGE

Pricing

$235/0.02 mg $375/0.1 mg $780/5x0.02 mg $825/0.5 mg $1,380/5x0.1 mg $1,600/2x0.5 mg $2,335/3x0.5 mg $3,025/4x0.5 mg $3,410/5x0.5 mg

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Bioactivity (Mouse LIFR alpha inhibits human LIF (Cat# ATGP3533) induced cell proliferation in the TF-1 human erythroleukemic cells. The ED50 range is )

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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LIFR alpha, Active Protein (Cat# AAA48367)

Full Name

Recombinant Mouse LIFR alpha Protein

Applications

Bioactivity, SDS-PAGE

Purity

>90% by SDS-PAGE

Pricing

$275/0.01 mg $355/0.02 mg $415/0.05 mg $600/0.1 mg $905/5x0.01 mg $945/0.25 mg $1,290/5x0.02 mg $1,560/5x0.05 mg $1,845/2x0.25 mg $2,380/5x0.1 mg $2,690/3x0.25 mg $3,490/4x0.25 mg $3,970/5x0.25 mg

What Are Active Proteins?

Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).

If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.

Key Applications of Active Proteins

1. Scientific Research

• Aid in the study of how proteins function in the body


• Aid in understanding various disease processes

2. Drug Development

• Powerful tools to investigate how potential drugs interact with specific proteins


• Ideal for identifying drug targets

3. Cell Culture

• Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)


• Can be used to promote cellular development into specific types (differentiation)

4. Diagnostics

• Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)


• Note: All products are strictly for research-use only (RUO).

5. Therapeutics

• Some active proteins are used directly as treatments (e.g., insulin, enzymes)


• Note: All products are strictly for research-use only (RUO).

6. Vaccine Development

• Used to create or test vaccines by mimicking parts of viruses or bacteria

7. Biochemical Assays

• They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests

Why Buy Active Proteins from AAA Biotech?

• High biological activity – Verified to perform as expected or indicated on datasheet


• Strict quality control – We are confident in our active proteins’ reliability and consistency


• High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components


• Freeze-dried for stability – Long shelf life and straightforward storage


• Mostly tag-free – Closer to natural/native protein form

FAQ

1. What are active proteins used for in research?

Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.

2. How are AAA Biotech's active proteins validated?

AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.

3. Are these proteins tested for biological activity?

Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.