Active Proteins

AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.

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Application Data (Immobilized human ACE2 protein (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) Protein (ECD, His Tag),the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) Protein (ECD, His Tag) is 100-600 ng/mL.)

SDS-PAGE

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COVID 19 Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) Protein (ECD, His Tag) Coronavirus, Active Protein (Cat# AAA258082)

Full Name

SARS-CoV-2 (2019-nCoV) Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) Protein (ECD, His Tag)

Purity

>85% as determined by SDS-PAGE

Pricing

$610/0.1 mg $1,180/2x0.1 mg $1,725/3x0.1 mg $2,235/4x0.1 mg $2,530/5x0.1 mg

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Bioactivity (Amphiregulin, also known as AREG, is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. Besides, Epidermal Growth Factor Receptor (EGFR) has been identified as an interactor of ARGE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARGE and recombinant human EGFR. Briefly, ARGE were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EGFR-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-ARGE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ARGE and EGFR was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of ARGE with EGFR.)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Amphiregulin, Active Protein (Cat# AAA150047)

Full Name

Active Amphiregulin (AREG)

Gene Names

AREG; AR; SDGF; AREGB; CRDGF

Applications

Activity Assay, Cell Culture

Purity

>90%

Pricing

$220/0.01 mg $410/0.05 mg $615/0.1 mg $750/0.2 mg $1,415/0.5 mg $2,100/1 mg $2,765/2 mg $5,110/5 mg $9,960/2x5 mg $14,550/3x5 mg $18,890/4x5 mg $23,100/5x5 mg

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Bioactivity (HTRF assay for AMPK Complex (A2+B2+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A2+B2+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF assay for AMPK Complex (A2+B2+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A2+B2+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant AMPK Complex (A2+B2+G2) protein 10% SDS-PAGE Coomassie staining MW: 63.7 kDa Purity: >= 80%)

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AMPK Complex (A2+B2+G2), Active Protein (Cat# AAA60320)

Full Name

Recombinant AMPK Complex (A2+B2+G2) protein

Purity

>=90%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

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Bioactivity (HTRF assay for AMPK Complex (A1+B1+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF assay for AMPK Complex (A1+B1+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant AMPK Complex (A1+B1+G1) protein 10% SDS-PAGE Coomassie staining MW: PRKAA1: 65.3 kDa, PRKAB1: 30.4 kDa, PRKAG2: 37.6 kDa Purity: >= 90%)

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AMPK Complex (A1+B1+G1), Active Protein (Cat# AAA60345)

Full Name

Recombinant AMPK Complex (A1+B1+G1) protein

Purity

>=90%

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Bioactivity

SDS-PAGE (Sample Purity Data.)

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ACE2, Active Protein (Cat# AAA73159)

Full Name

ACE2 (19-740) Protein

Gene Names

ACE2; ACEH

Pricing

$535/0.01 mg $615/0.02 mg $715/0.05 mg $955/0.1 mg $1,860/2x0.1 mg $2,715/3x0.1 mg $3,375/5x0.1 mg $3,525/4x0.1 mg

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Bioactivity (Afamin (AFM, also known as Alpha -Albumin), a bioactive protein, is expressed in the liver and secreted into the bloodstream. Human afamin is a 87 kDa protein which is encoded by the fourth member of the human albumin gene family. Mouse AFM shares 66% aa sequence identity with Full-length human AFM. This protein can bind and transport vitamin E across the blood-brain barrier in body fluids under conditions where the lipoprotein system is not sufficient, it also can act as a carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse AFM and recombinant human WNT3A. Briefly, AFM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to WNT3A-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AFM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse AFM and recombinant human WNT3A was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Afamin (AFM), Active Protein (Cat# AAA161881)

Full Name

Active Afamin (AFM)

Gene Names

Afm; Alf; alpha-Alb

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$240/0.01 mg $455/0.05 mg $690/0.1 mg $845/0.2 mg $1,615/0.5 mg $2,380/1 mg $3,155/2 mg $5,820/5 mg $11,345/2x5 mg $16,585/3x5 mg $21,525/4x5 mg $26,345/5x5 mg

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Bioactivity

SDS-PAGE

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Angiotensin-Converting Enzyme 2/ACE-2, Active Protein (Cat# AAA177978)

Full Name

Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-10His)

Purity

>95% as determined by reducing SDS-PAGE

Pricing

$295/0.01 mg $840/0.05 mg $1,630/2x0.05 mg $2,380/3x0.05 mg $3,085/4x0.05 mg $3,875/5x0.05 mg

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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Acid phosphatase 1, Active Protein (Cat# AAA48522)

Full Name

Acid phosphatase 1,1-158aa, Human, His tag, E Coli (Bioactivity Validated)

Gene Names

ACP1; HAAP; LMWPTP; LMW-PTP

Applications

Enzyme Activity, SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

$250/0.02 mg $440/0.1 mg $860/5x0.02 mg $1,060/0.5 mg $1,690/5x0.1 mg $2,055/2x0.5 mg $3,000/3x0.5 mg $3,895/4x0.5 mg $4,450/5x0.5 mg

SDS-PAGE

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AKR7A2, Active Protein (Cat# AAA48568)

Full Name

AKR7A2, 1-359aa, Human, His tag, E. coli (Bioactivity Validated)

Gene Names

AKR7A2; AFAR; AKR7; AFAR1; AFB1-AR1

Applications

SDS-PAGE

Purity

> 90% by SDS-PAGE

Pricing

$250/0.02 mg $440/0.1 mg $860/5x0.02 mg $1,060/0.5 mg $1,690/5x0.1 mg $2,055/2x0.5 mg $3,000/3x0.5 mg $3,895/4x0.5 mg $4,450/5x0.5 mg

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Bioactivity (Figure 2. Cell apoptosis of A549 cells after stimulated with IFNb.)

Bioactivity (Interferon Beta (IFNb) is belongs to type I interferons (IFNs) family which a large subgroup of interferon proteins that help regulate the activity of the immune system.The IFNb proteins are produced in large quantities by fibroblasts. They have antiviral)

SDS-PAGE (Figure. SDS-PAGE)

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Interferon Beta (IFNb), Active Protein (Cat# AAA153003)

Full Name

Active Interferon Beta (IFNb)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$340/0.01 mg $725/0.05 mg $1,110/0.1 mg $1,370/0.2 mg $2,660/0.5 mg $3,930/1 mg $5,215/2 mg $9,595/5 mg $18,710/2x5 mg $27,345/3x5 mg $35,500/4x5 mg

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Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2 ug/ml (100 ul/well) can bind Human RNF43 hFc, the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL)

SDS-PAGE

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RNF43, Active Protein (Cat# AAA258047)

Full Name

RNF43 Protein, Human, Recombinant (hFc Tag)

Gene Names

RNF43; URCC; RNF124

Purity

>90% as determined by SDS-PAGE

Pricing

$435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,605/4x0.1 mg $1,755/5x0.1 mg

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Application Data (Figure. Cell proliferation of Hela cells after stimulated with ANXA2.)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Annexin A2, Active Protein (Cat# AAA150121)

Full Name

Active Annexin A2 (ANXA2)

Gene Names

ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

$285/0.01 mg $565/0.05 mg $845/0.1 mg $1,040/0.2 mg $1,995/0.5 mg $2,960/1 mg $3,900/2 mg $7,205/5 mg $14,045/2x5 mg $20,525/3x5 mg $26,645/4x5 mg

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Bioactivity (Transglutaminase 2 (TGM2), encoded by the TGM2 gene, is belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions. In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP and has intrinsic kinase activity. TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases. The activity of recombinant human TGM2 is measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH in the assay buffer 200 mM MES, 10 mM DTT, 10 mM CaCl2, 100 mM Hydroxylamine Hydrochloride, pH 6.0. The rhTGM2 is diluted to 12.5 ug/ml in assay buffer. Loading into a clear well plate 50 uL of 12.5 ug/mL rhTGM2 and start the reaction by adding 50 uL of 100 mM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhTGM2. Incubated at 37 degree C for 2 hours and stop the reaction with 400 ul stop solution of 0.37 M FeCl3, 0.67 M HCl, 0.2 M Trichloroacetic Acid. Centrifuge at 2000 rpm for 2 minutes and then load 200 ul of the supernatant into a plate and read at 525 nm (absorbance) in endpoint mode. The specific activity of recombinant human TGM2 is > 800 pmol/min/ug.)

SDS-PAGE (Figure. SDS-PAGE)

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Transglutaminase 2 (TGM2), Active Protein (Cat# AAA161864)

Full Name

Active Transglutaminase 2 (TGM2)

Gene Names

TGM2; TG2; TGC; GNAH; G-ALPHA-h

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$205/0.01 mg $360/0.05 mg $525/0.1 mg $645/0.2 mg $1,205/0.5 mg $1,760/1 mg $2,325/2 mg $4,260/5 mg $8,305/2x5 mg $12,130/3x5 mg $15,750/4x5 mg $19,295/5x5 mg

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Bioactivity (HTRF assay for AMPK Complex (A2+B2+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A2+B2+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 ul ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF assay for AMPK Complex (A2+B2+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A2+B2+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 ul ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant AMPK Complex (A2+B2+G1) protein 10% SDS-PAGE Coomassie staining MW: PRKAA2: 63.6 kDa, PRKAB2: 30.3 kDa, PRKAG1: 37.6 kDa Purity: >= 90%)

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AMPK Complex (A2+B2+G1), Active Protein (Cat# AAA60319)

Full Name

Recombinant AMPK Complex (A2+B2+G1) protein

Purity

>=90%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)

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Alphaenolase, Active Protein (Cat# AAA75217)

Full Name

Alphaenolase protein

Applications

SDS-PAGE

Purity

> 90% pure

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,425/5x0.1 mg

What Are Active Proteins?

Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).

If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.

Key Applications of Active Proteins

1. Scientific Research

• Aid in the study of how proteins function in the body


• Aid in understanding various disease processes

2. Drug Development

• Powerful tools to investigate how potential drugs interact with specific proteins


• Ideal for identifying drug targets

3. Cell Culture

• Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)


• Can be used to promote cellular development into specific types (differentiation)

4. Diagnostics

• Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)


• Note: All products are strictly for research-use only (RUO).

5. Therapeutics

• Some active proteins are used directly as treatments (e.g., insulin, enzymes)


• Note: All products are strictly for research-use only (RUO).

6. Vaccine Development

• Used to create or test vaccines by mimicking parts of viruses or bacteria

7. Biochemical Assays

• They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests

Why Buy Active Proteins from AAA Biotech?

• High biological activity – Verified to perform as expected or indicated on datasheet


• Strict quality control – We are confident in our active proteins’ reliability and consistency


• High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components


• Freeze-dried for stability – Long shelf life and straightforward storage


• Mostly tag-free – Closer to natural/native protein form

FAQ

1. What are active proteins used for in research?

Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.

2. How are AAA Biotech's active proteins validated?

AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.

3. Are these proteins tested for biological activity?

Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.