Active Proteins

AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.

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Bioactivity (Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit doe)

SDS-PAGE (Figure. SDS-PAGE)

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Interleukin 3 Receptor Alpha (IL3Ra), Active Protein (Cat# AAA153089)

Full Name

Active Interleukin 3 Receptor Alpha (IL3Ra)

Gene Names

IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$320/0.01 mg $670/0.05 mg $1,015/0.1 mg $1,255/0.2 mg $2,440/0.5 mg $3,605/1 mg $4,780/2 mg $8,795/5 mg $17,150/2x5 mg $25,060/3x5 mg $32,535/4x5 mg

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Bioactivity (Antithrombin III, also known as Serpin C1, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases. For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery. The activity of recombinant human antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 ug/ml in the assay buffer and 10 ul different concentrations of recombinant human antithrombin III (MW: 50 KD) was incubated with 10 ul diluted thrombin at 37 degree C for 30 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human antithrombin III significantly decreased thrombin activity. The inhibition IC50 was )

SDS-PAGE (Figure. SDS-PAGE)

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Antithrombin (AT), Active Protein (Cat# AAA161886)

Full Name

Active Antithrombin (AT)

Gene Names

SERPINC1; AT3; AT3D; ATIII; THPH7

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$340/0.01 mg $705/0.05 mg $1,095/0.1 mg $1,340/0.2 mg $2,605/0.5 mg $3,855/1 mg $5,120/2 mg $9,425/5 mg $18,380/2x5 mg $26,855/3x5 mg $34,860/4x5 mg

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Application Data (1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 ug/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 ug/ml.)

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CD47, Active Protein (Cat# AAA258008)

Full Name

CD47 Protein, Human, Recombinant (ECD, hFc Tag)

Gene Names

CD47; IAP; OA3; MER6

Purity

>95% as determined by SDS-PAGE

Pricing

$435/0.1 mg $2,310/1 mg $4,500/2x1 mg $6,570/3x1 mg $8,530/4x1 mg $10,195/5x1 mg

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Bioactivity (Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 10 ug recombinant human Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 1), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)

SDS-PAGE (Figure. SDS-PAGE)

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Annexin V (ANXA5), Active Protein (Cat# AAA161723)

Full Name

Active Annexin V (ANXA5)

Gene Names

ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$315/0.01 mg $660/0.05 mg $1,005/0.1 mg $1,240/0.2 mg $2,410/0.5 mg $3,550/1 mg $4,720/2 mg $8,680/5 mg $16,915/2x5 mg $24,715/3x5 mg $32,090/4x5 mg

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Bioactivity (Figure. The binding activity of SYK with SIGLEC2.Spleen Tyrosine Kinase (SYK) is a member of the SYK family of tyrosine kinases. These non-receptor cytoplasmic tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. Within B and T cells respectively, SYK and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. SYK plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. Besides, Sialic Acid Binding Ig Like Lectin 1 (SIGLEC2) has been identified as an interactor of SYK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SYK and recombinant human SIGLEC2. Briefly, SYK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-SYK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SYK and SIGLEC2 was shown in Figure 1, and this effect was in a dose dependent manner.)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Spleen Tyrosine Kinase, Active Protein (Cat# AAA150134)

Full Name

Active Spleen Tyrosine Kinase (SYK)

Gene Names

SYK; p72-Syk

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

$335/0.01 mg $680/0.05 mg $1,055/0.1 mg $1,295/0.2 mg $2,500/0.5 mg $3,695/1 mg $4,900/2 mg $9,025/5 mg $17,590/2x5 mg $25,710/3x5 mg $33,380/4x5 mg

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Bioactivity (A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD() that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 ul assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.)

SDS-PAGE (Figure. SDS-PAGE)

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Lactate Dehydrogenase C (LDHC), Active Protein (Cat# AAA161921)

Full Name

Active Lactate Dehydrogenase C (LDHC)

Gene Names

Ldhc; Ldh3

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$290/0.01 mg $575/0.05 mg $860/0.1 mg $1,065/0.2 mg $2,035/0.5 mg $3,020/1 mg $3,990/2 mg $7,365/5 mg $14,360/2x5 mg $20,985/3x5 mg $27,240/4x5 mg

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Application Data (Measured by its ability to bind human CD32a-His in a functional ELISA.)

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IgG2, Active Protein (Cat# AAA258022)

Full Name

IgG2 Protein, Human, Recombinant (257 Ser/Ala)

Purity

>90% as determined by SDS-PAGE

Pricing

$210/0.1 mg $760/1 mg $1,485/2x1 mg $2,170/3x1 mg $2,820/4x1 mg $3,240/5x1 mg

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Application Data (Measured by its binding ability in a functional ELISA. Immobilized human B7-H6-His at 10 ug/mL (100 uL/well) can bind human NCR3-Fch, the EC50 of human NCR3-Fch is 6-200 ng/mL.)

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B7-H6, Active Protein (Cat# AAA258051)

Full Name

B7-H6 Protein, Human, Recombinant (His Tag)

Gene Names

NCR3LG1; B7H6; B7-H6; DKFZp686O24166

Purity

>95% as determined by SDS-PAGE

Pricing

$310/0.05 mg $1,415/0.5 mg $2,755/2x0.5 mg $4,020/3x0.5 mg $5,220/4x0.5 mg $6,165/5x0.5 mg

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Application Data (Figure. Cell proliferation of Raji cells after stimulated with IL6.Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 which contains various concentrations of recombinant dog IL6. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL6 for 5 days. The result was shown in Figure 2. It was obvious that IL6 significantly increased cell viability of Raji cells.(A) Raji cells cultured in 1640, stimulated with 1ng/mL IL6 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Interleukin 6, Active Protein (Cat# AAA150062)

Full Name

Active Interleukin 6 (IL6)

Gene Names

IL6; IL-6

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

$335/0.01 mg $695/0.05 mg $1,070/0.1 mg $1,315/0.2 mg $2,560/0.5 mg $3,770/1 mg $5,010/2 mg $9,210/5 mg $17,950/2x5 mg $26,230/3x5 mg $34,055/4x5 mg

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Bioactivity (HTRF assay for AMPK Complex (A1+B1+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF assay for AMPK Complex (A1+B1+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant AMPK Complex (A1+B1+G2) protein 10% SDS-PAGE Coomassie staining MW: PRKAA1: 65.3 kDa, PRKAB1: 30.4, PRKAG2: 63.7 kDa. Purity >80 %)

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AMPK Complex (A1+B1+G2), Active Protein (Cat# AAA60344)

Full Name

Recombinant AMPK Complex (A1+B1+G2) protein

Purity

>=80%

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Bioactivity (Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant chicken CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)

SDS-PAGE (Figure. SDS-PAGE)

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Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161775)

Full Name

Active Carbonic Anhydrase II (CA2)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$200/0.01 mg $340/0.05 mg $480/0.1 mg $590/0.2 mg $1,090/0.5 mg $1,595/1 mg $2,100/2 mg $3,845/5 mg $7,490/2x5 mg $10,945/3x5 mg $14,210/4x5 mg $17,390/5x5 mg

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Bioactivity (Serum Amyloid A2 (SAA2) is a multifunctional apolipoprotein that belongs to SAA family. SAA2 is produced by hepatocytes in response to pro-inflammatory cytokines and is the most prominent members of the acute phase response (APR) during which their serum levels rise dramatically after trauma, infection and other stimuli. Besides, Apolipoprotein C3 (APOC3) has been identified as an interactor of SAA2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SAA2 and recombinant human APOC3. Briefly, SAA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOC3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SAA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SAA2 and recombinant human APOC3 was shown in Figure 1, and this effect was in a dose dependent manner.)

SDS-PAGE (Figure. SDS-PAGE)

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Serum Amyloid A2 (SAA2), Active Protein (Cat# AAA161860)

Full Name

Active Serum Amyloid A2 (SAA2)

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$215/0.01 mg $390/0.05 mg $590/0.1 mg $705/0.2 mg $1,335/0.5 mg $1,965/1 mg $2,600/2 mg $4,790/5 mg $9,335/2x5 mg $13,645/3x5 mg $17,715/4x5 mg $21,675/5x5 mg

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Bioactivity (HTRF assay for AMPK Complex (A1+B2+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B2+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF assay for AMPK Complex (A1+B2+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B2+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant AMPK Complex (A1+B2+G2) protein 10% SDS-PAGE Coomassie staining MW: PRKAA1: 65.3 kDa, PRKAB2: 30 kDa, PRKAG2: 63.7 kDa Purity: >= 90%)

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AMPK Complex (A1+B2+G2), Active Protein (Cat# AAA60318)

Full Name

Recombinant AMPK Complex (A1+B2+G2) protein

Purity

>=90%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

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Application Data (Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.6-3 ug/mL.)

SDS-PAGE

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FGF17, Active Protein (Cat# AAA258010)

Full Name

FGF17 Protein, Human, Recombinant

Gene Names

FGF17; HH20; FGF-13; FGF-17

Purity

>95% as determined by SDS-PAGE

Pricing

$230/0.02 mg $490/0.1 mg $2,745/1 mg $5,350/2x1 mg $7,815/3x1 mg $10,145/4x1 mg $12,165/5x1 mg

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Application Data (Measured by its ability to induce apoptosis of TF1 human erythroleukemic cells.The ED50 for this effect is typically 20-120 ng/mL.)

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TL1A, Active Protein (Cat# AAA258057)

Full Name

TL1A Protein, Human, Recombinant (rFc Tag)

Gene Names

TNFSF15; TL1; TL1A; VEGI; VEGI192A

Purity

>90% as determined by SDS-PAGE

Pricing

$490/0.1 mg $2,680/1 mg $5,220/2x1 mg $7,625/3x1 mg $9,895/4x1 mg $11,850/5x1 mg

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Bioactivity (SARS-CoV-2 Spike RBD is coated at 5 ug/ml (100 ul/well) can bind Human ACE-2. The ED50 range )

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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ACE-2, Active Protein (Cat# AAA48372)

Full Name

Recombinant human ACE-2 protein

Gene Names

ACE2; ACEH

Applications

Enzyme Activity, SDS-PAGE

Purity

>90% by SDS-PAGE

Pricing

$310/0.01 mg $400/0.02 mg $580/0.05 mg $875/0.1 mg $1,085/5x0.01 mg $1,410/0.25 mg $1,500/5x0.02 mg $2,305/5x0.05 mg $2,330/0.5 mg $3,620/5x0.1 mg $4,540/2x0.5 mg $6,050/5x0.25 mg $6,630/3x0.5 mg $8,605/4x0.5 mg $10,180/5x0.5 mg

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Bioactivity (Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 5 ug recombinant human Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 1), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)

SDS-PAGE (Figure. SDS-PAGE)

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Annexin V (ANXA5), Active Protein (Cat# AAA161722)

Full Name

Active Annexin V (ANXA5)

Gene Names

ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$315/0.01 mg $660/0.05 mg $1,005/0.1 mg $1,240/0.2 mg $2,410/0.5 mg $3,550/1 mg $4,720/2 mg $8,680/5 mg $16,915/2x5 mg $24,715/3x5 mg $32,090/4x5 mg

What Are Active Proteins?

Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).

If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.

Key Applications of Active Proteins

1. Scientific Research

• Aid in the study of how proteins function in the body


• Aid in understanding various disease processes

2. Drug Development

• Powerful tools to investigate how potential drugs interact with specific proteins


• Ideal for identifying drug targets

3. Cell Culture

• Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)


• Can be used to promote cellular development into specific types (differentiation)

4. Diagnostics

• Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)


• Note: All products are strictly for research-use only (RUO).

5. Therapeutics

• Some active proteins are used directly as treatments (e.g., insulin, enzymes)


• Note: All products are strictly for research-use only (RUO).

6. Vaccine Development

• Used to create or test vaccines by mimicking parts of viruses or bacteria

7. Biochemical Assays

• They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests

Why Buy Active Proteins from AAA Biotech?

• High biological activity – Verified to perform as expected or indicated on datasheet


• Strict quality control – We are confident in our active proteins’ reliability and consistency


• High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components


• Freeze-dried for stability – Long shelf life and straightforward storage


• Mostly tag-free – Closer to natural/native protein form

FAQ

1. What are active proteins used for in research?

Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.

2. How are AAA Biotech's active proteins validated?

AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.

3. Are these proteins tested for biological activity?

Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.