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Application

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Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.


Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.


Viewing 8900-8950 of 98831 product results


product-image-AAA324043_WB15.png WB (Western Blot) (Western blot analysis of extracts from Mouse brain, using DCLK2 Ab. The lane on the left was treated with blocking peptide.)

DCLK2, Polyclonal Antibody (Cat# AAA324043)

Full Name
DCLK2 Antibody
Gene Names
DCLK2; CL2; DCK2; CLIK2; DCDC3; CLICK2; DCDC3B; DCAMKL2; CLICK-II
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
product-image-AAA324045_IHC11.jpg IHC (Immunohistochemisry) (AAA324045 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CAMK5, Polyclonal Antibody (Cat# AAA324045)

Full Name
CAMK5 Antibody
Gene Names
CAMKV; 1G5; VACAMKL
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324047_IF13.jpg IF (Immunofluorescence) (AAA324047 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

ACRBP, Polyclonal Antibody (Cat# AAA324047)

Full Name
ACRBP Antibody
Gene Names
ACRBP; CT23; SP32; OY-TES-1
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324049_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from COLO cells using PPHLN antibody.The lane on the left is treated with the antigen-specific peptide.)

PPHLN, Polyclonal Antibody (Cat# AAA324049)

Full Name
PPHLN Antibody
Gene Names
PPHLN1; CR; HSPC206; HSPC232
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324053_IF13.jpg IF (Immunofluorescence) (AAA324053 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IPMK, Polyclonal Antibody (Cat# AAA324053)

Full Name
IPMK Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324057_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HUVEC cells using SMRC2 antibody.)

SMRC2, Polyclonal Antibody (Cat# AAA324057)

Full Name
SMRC2 Antibody
Gene Names
SMARCC2; Rsc8; BAF170; CRACC2
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324064_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using GRK7 antibody.The lane on the left is treated with the antigen-specific peptide.)

GRK7, Polyclonal Antibody (Cat# AAA324064)

Full Name
GRK7 Antibody
Gene Names
GRK7; GPRK7
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324065_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COS-7 cells, using DUS19 antibody.)

DUSP19, Polyclonal Antibody (Cat# AAA324065)

Full Name
DUSP19 Antibody
Gene Names
DUSP19; SKRP1; DUSP17; LMWDSP3; TS-DSP1
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324066_WB10.jpg WB (Western Blot) (Western blot analysis of extracts from HepG2 cells using TF3C2 antibody.)

GTF3C2, Polyclonal Antibody (Cat# AAA324066)

Full Name
GTF3C2 Antibody
Gene Names
GTF3C2; TFIIIC110; TFIIIC-BETA
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324071_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using SMG7 antibody.)

SMG7, Polyclonal Antibody (Cat# AAA324071)

Full Name
SMG7 Antibody
Gene Names
SMG7; EST1C; SGA56M; C1orf16
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324079_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from mouse Brian, using MEKKK 1 antibody.)

MEKKK 1, Polyclonal Antibody (Cat# AAA324079)

Full Name
MEKKK 1 Antibody
Gene Names
MAP4K1; HPK1
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324080_IF13.jpg IF (Immunofluorescence) (AAA324080 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

FOXJ1, Polyclonal Antibody (Cat# AAA324080)

Full Name
FOXJ1 Antibody
Gene Names
FOXJ1; HFH4; HFH-4; FKHL13
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA324081_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COLO cells, using DLX4 antibody.)

DLX4, Polyclonal Antibody (Cat# AAA324081)

Full Name
DLX4 Antibody
Gene Names
DLX4; BP1; DLX7; DLX8; DLX9; OFC15
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324083_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COLO cells, using CNKR1 antibody.The lane on the left is treated with the antigen-specific peptide.)

CNKSR1, Polyclonal Antibody (Cat# AAA324083)

Full Name
CNKSR1 Antibody
Gene Names
CNKSR1; CNK; CNK1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324089_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, using MOBKL2A/B antibody.)

MOBKL2A/B, Polyclonal Antibody (Cat# AAA324089)

Full Name
MOBKL2A/B Antibody
Gene Names
MOB3A; MOB1C; moblak; MOB-LAK; MOBKL2A
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324091_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HT-29 cells, using IP3KC antibody.)

IP3KC, Polyclonal Antibody (Cat# AAA324091)

Full Name
IP3KC Antibody
Gene Names
ITPKC; IP3KC; IP3-3KC
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324093_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells, using PGBD1 antibody.The lane on the left is treated with the antigen-specific peptide.)

PGBD1, Polyclonal Antibody (Cat# AAA324093)

Full Name
PGBD1 Antibody
Gene Names
PGBD1; SCAND4; HUCEP-4; dJ874C20.4
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324097_WB10.jpg WB (Western Blot) (Western blot analysis of extracts from mouse muscle, using IP6K3 Antibody.)

IP6K3, Polyclonal Antibody (Cat# AAA324097)

Full Name
IP6K3 Antibody
Gene Names
IP6K3; IHPK3; INSP6K3
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324099_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HuvEc cells using AL2S7 antibody.)

AL2S7, Polyclonal Antibody (Cat# AAA324099)

Full Name
AL2S7 Antibody
Gene Names
CDK15; PFTK2; ALS2CR7; PFTAIRE2
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324100_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells, using CIB3 antibody.)

CIB3, Polyclonal Antibody (Cat# AAA324100)

Full Name
CIB3 Antibody
Gene Names
CIB3; KIP3
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324106_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from mouse brain, using ATF6B Antibody.)

ATF6B, Polyclonal Antibody (Cat# AAA324106)

Full Name
ATF6B Antibody
Gene Names
ATF6B; G13; CREBL1; CREB-RP
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324109_IF13.jpg IF (Immunofluorescence) (AAA324109 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

TINF2, Polyclonal Antibody (Cat# AAA324109)

Full Name
TINF2 Antibody
Gene Names
TINF2; TIN2; DKCA3
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA324110_IF13.jpg IF (Immunofluorescence) (AAA324110 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

MMTAG2, Polyclonal Antibody (Cat# AAA324110)

Full Name
MMTAG2 Antibody
Gene Names
C1orf35; MMTAG2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA324113_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HUVEC cells, using NADAP antibody.The lane on the left is treated with the antigen-specific peptide.)

NADAP, Polyclonal Antibody (Cat# AAA324113)

Full Name
NADAP Antibody
Gene Names
SLC4A1AP; HLC3
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323702_WB15.jpg WB (Western Blot) (Western blot analysis of Phospho-DRP1 (Ser616) using HT-29 whole cell lysates)

DRP1, Polyclonal Antibody (Cat# AAA323702)

Full Name
Phospho-DRP1 (Ser616) Antibody
Gene Names
DNM1L; DLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA323709_WB13.jpg WB (Western Blot) (Western blot analysis of Phospho-p57 Kip2 (Thr310) using HUVEC whole cell lysates)

p57 Kip2, Polyclonal Antibody (Cat# AAA323709)

Full Name
Phospho-p57 Kip2 (Thr310) Antibody
Gene Names
CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA323710_WB13.jpg WB (Western Blot) (Western blot analysis of p57 Kip2 using 293 whole cell lysates)

p57 Kip2, Polyclonal Antibody (Cat# AAA323710)

Full Name
p57 Kip2 Antibody
Gene Names
CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323711_WB13.jpg WB (Western Blot) (Western blot analysis of LRP6 using HepG2 whole cell lysates)

LRP6, Polyclonal Antibody (Cat# AAA323711)

Full Name
LRP6 Antibody
Gene Names
LRP6; ADCAD2; STHAG7
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323723_IF11.jpg IF (Immunofluorescence) (AAA323723 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

TP53I11, Polyclonal Antibody (Cat# AAA323723)

Full Name
TP53I11 Antibody
Gene Names
TP53I11; PIG11
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323724_IF13.jpg IF (Immunofluorescence) (AAA323724 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

OVOL1, Polyclonal Antibody (Cat# AAA323724)

Full Name
OVOL1 Antibody
Gene Names
OVOL1; HOVO1
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323727_IF13.jpg IF (Immunofluorescence) (AAA323727 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

BCKD, Polyclonal Antibody (Cat# AAA323727)

Full Name
BCKD Antibody
Gene Names
BCKDK; BDK; BCKDKD
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323729_IF11.jpg IF (Immunofluorescence) (AAA323729 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

HGS, Polyclonal Antibody (Cat# AAA323729)

Full Name
HGS Antibody
Gene Names
HGS; HRS
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323736_IF13.jpg IF (Immunofluorescence) (AAA323736 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

TBX1, Polyclonal Antibody (Cat# AAA323736)

Full Name
TBX1 Antibody
Gene Names
TBX1; DGS; TGA; VCF; CAFS; CTHM; DGCR; DORV; VCFS; TBX1C; CATCH22
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323738_IHC11.jpg IHC (Immunohistochemisry) (AAA323738 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

AKAP8, Polyclonal Antibody (Cat# AAA323738)

Full Name
AKAP8 Antibody
Gene Names
AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323739_IF13.jpg IF (Immunofluorescence) (AAA323739 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

AIRE, Polyclonal Antibody (Cat# AAA323739)

Full Name
AIRE Antibody
Gene Names
AIRE; APS1; APSI; PGA1; AIRE1; APECED
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323741_IF13.jpg IF (Immunofluorescence) (AAA323741 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

ZNF387, Polyclonal Antibody (Cat# AAA323741)

Full Name
ZNF387 Antibody
Gene Names
ST18; NZF3; ZNF387; ZC2H2C3; ZC2HC10
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323750_IF13.jpg IF (Immunofluorescence) (AAA323750 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

PFDN1, Polyclonal Antibody (Cat# AAA323750)

Full Name
PFDN1 Antibody
Gene Names
PFDN1; PDF; PFD1
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323757_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HuvEc cells using SUPT3 antibody.)

SUPT3H, Polyclonal Antibody (Cat# AAA323757)

Full Name
SUPT3H Antibody
Gene Names
SUPT3H; SPT3; SPT3L
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323760_IF11.jpg IF (Immunofluorescence) (AAA323760 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

BCAR3, Polyclonal Antibody (Cat# AAA323760)

Full Name
BCAR3 Antibody
Gene Names
BCAR3; NSP2; AND-34; SH2D3B
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA323764_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells, using ALX3 antibody.)

ALX3, Polyclonal Antibody (Cat# AAA323764)

Full Name
ALX3 Antibody
Gene Names
ALX3; FND; FND1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323773_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COLO205 cells using ZNF682 antibody.)

ZNF682, Polyclonal Antibody (Cat# AAA323773)

Full Name
ZNF682 Antibody
Gene Names
ZNF682; BC39498_3
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323777_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells using ACL6A antibody.)

ACTL6A, Polyclonal Antibody (Cat# AAA323777)

Full Name
ACTL6A Antibody
Gene Names
ACTL6A; Arp4; ACTL6; BAF53A; INO80K; ARPN-BETA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323558_IHC13.jpg IHC (Immunohiostchemistry) (AAA323558 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR133, Polyclonal Antibody (Cat# AAA323558)

Full Name
GPR133 Antibody
Gene Names
ADGRD1; PGR25; GPR133
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323559_IHC13.jpg IHC (Immunohiostchemistry) (AAA323559 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR146, Polyclonal Antibody (Cat# AAA323559)

Full Name
GPR146 Antibody
Gene Names
GPR146; PGR8
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323563_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Hela, using GPR21 Antibody. Lane 1 was treated with the blocking peptide.)

GPR21, Polyclonal Antibody (Cat# AAA323563)

Full Name
GPR21 Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323564_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Hepg2, using GPR27 Antibody. Lane 1 was treated with the blocking peptide.)

GPR27, Polyclonal Antibody (Cat# AAA323564)

Full Name
GPR27 Antibody
Gene Names
GPR27; SREB1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323566_IHC13.jpg IHC (Immunohiostchemistry) (AAA323566 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR32, Polyclonal Antibody (Cat# AAA323566)

Full Name
GPR32 Antibody
Gene Names
GPR32; RVDR1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323576_WB13.jpg WB (Western Blot) (Western blot analysis of GPR68 expression in Human fetal brain lysate, The lane on the left is treated with the antigen-specific peptide.)

GPR68, Polyclonal Antibody (Cat# AAA323576)

Full Name
GPR68 Antibody
Gene Names
GPR68; OGR1; AI2A6; GPR12A
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323579_WB13.jpg WB (Western Blot) (Western blot analysis of GPR78 expression in OVCAR 3 cell lysate, The lane on the left is treated with the antigen-specific peptide.)

GPR78, Polyclonal Antibody (Cat# AAA323579)

Full Name
GPR78 Antibody
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323580_IHC10.jpg IHC (Immunohistochemistry) (AAA323580 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR8, Polyclonal Antibody (Cat# AAA323580)

Full Name
GPR8 Antibody
Gene Names
NPBWR2; GPR8
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing

What are Polyclonal Antibodies?


Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).


Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.


Key Uses of Polyclonal Antibodies


  • Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

  • Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

  • ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

  • Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

  • Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.


Why Buy Polyclonal Antibodies from AAA Biotech?


1. Ideal for Various Applications


Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.


2. Rigorous Quality Control


All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.


3. Wide Assortment of Antibodies


Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.


4. Highly Purified


Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.


FAQ


1. How are polyclonal antibodies produced?


Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.


2. How do polyclonal antibodies differ from monoclonal antibodies?


Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.


3. How should I store polyclonal antibodies?


Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.


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