Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using ENO2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ENO2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

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ENO2, Polyclonal Antibody (Cat# AAA37019)

Full Name

ENO2 Polyclonal Antibody

Gene Names

ENO2; NSE; HEL-S-279

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using KRT8 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human esophagus using KRT8 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using KRT8 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KRT8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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KRT8, Polyclonal Antibody (Cat# AAA37027)

Full Name

KRT8 Polyclonal Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PITX2 antibody (AAA37029) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

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PITX2, Polyclonal Antibody (Cat# AAA37029)

Full Name

PITX2

Gene Names

PITX2; RS; RGS; ARP1; Brx1; IDG2; IGDS; IHG2; PTX2; RIEG; IGDS2; IRID2; Otlx2; RIEG1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ARF1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)

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ARF1, Polyclonal Antibody (Cat# AAA37031)

Full Name

ARF1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MCM3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MCM3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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MCM3, Polyclonal Antibody (Cat# AAA37034)

Full Name

MCM3 Polyclonal Antibody

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Reactivity

Human, Mouse, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CFB antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CFB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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CFB, Polyclonal Antibody (Cat# AAA37036)

Full Name

CFB Polyclonal Antibody

Gene Names

CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TNK2 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TNK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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TNK2, Polyclonal Antibody (Cat# AAA37038)

Full Name

TNK2 Polyclonal Antibody

Gene Names

TNK2; ACK; ACK1; ACK-1; p21cdc42Hs

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat heart using RAF1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat liver using RAF1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RAF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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RAF1, Polyclonal Antibody (Cat# AAA36715)

Full Name

RAF1 Polyclonal Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using TNFRSF6B antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human kidney cancer using TNFRSF6B antibody at dilution of 1:200 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TNFRSF6B antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HT-29 cells, using TNFRSF6B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)

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TNFRSF6B, Polyclonal Antibody (Cat# AAA36716)

Full Name

TNFRSF6B Polyclonal Antibody

Gene Names

TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PRF1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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PRF1, Polyclonal Antibody (Cat# AAA36732)

Full Name

PRF1 Polyclonal antibody

Gene Names

PRF1; P1; PFP; FLH2; PFN1; HPLH2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using GRIN2A antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GRIN2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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NMDAR2A, Polyclonal Antibody (Cat# AAA36788)

Full Name

NMDAR2A

Gene Names

GRIN2A; EPND; NR2A; GluN2A; NMDAR2A

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human kidney using MAP2K2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse brain, using MAP2K2 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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MAP2K2, Polyclonal Antibody (Cat# AAA36644)

Full Name

MAP2K2 Polyclonal Antibody

Gene Names

MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using CHEK2 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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Chk2, Polyclonal Antibody (Cat# AAA36668)

Full Name

Chk2 Rabbit pab Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

WB (Western Blot)

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CXCR3, Polyclonal Antibody (Cat# AAA75856)

Full Name

CXCR3 Antibody FITC-Conjugated

Gene Names

CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Dot Blot, Confocal Microscopy

Pricing

$625/0.1 mg $1,210/2x0.1 mg $1,765/3x0.1 mg $2,295/4x0.1 mg $2,575/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Cyclin G1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Cyclin G1 expression in DLD (A), Jurkat (B), mouse liver (C), rat liver (D) whole cell lysates.)

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Cyclin G1, Polyclonal Antibody (Cat# AAA104228)

Full Name

Anti-Cyclin G1 Antibody

Gene Names

CCNG1; CCNG

Reactivity

Human, Mouse, Rat, Bovine, Pig

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Nibrin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Nibrin expression in HeLa (A), HL60 (B) whole cell lysates.)

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Nibrin, Polyclonal Antibody (Cat# AAA104567)

Full Name

Anti-Nibrin Antibody

Gene Names

NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using PSR antibody at a dilution of 1:100)

WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 10 % SDS PAGE gel and PSR antibody at a dilution of 1:1000)

WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and PSR antibody at a dilution of 1:1000)

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PSR, Polyclonal Antibody (Cat# AAA107806)

Full Name

PSR antibody

Applications

Western Blot, Immunohistochemistry

Purity

PSR antibody was purified by antigen-affinity chromatography

Pricing

$1,375/0.1 mL $2,675/2x0.1 mL $3,905/3x0.1 mL $5,065/4x0.1 mL $5,950/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic granule. Endoplasmic reticulum lumen.)

WB (Western Blot) (Western blot analysis of multiple cell lines lysates showing detection of Calreticulin protein using Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122) at 1:5000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

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Calreticulin, Polyclonal Antibody (Cat# AAA103670)

Full Name

Calreticulin Antibody: FITC

Gene Names

CALR; RO; CRT; SSA; cC1qR; HEL-S-99n

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Fish (carp), Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep

Applications

Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Pricing

$560/0.2 mL $1,080/2x0.2 mL $1,580/3x0.2 mL $2,050/4x0.2 mL $2,195/5x0.2 mL

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IF (Immunofluorescence) (Figure 4. IF analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (AAA126971) and anti-Beta Tubulin antibody (M01857-3).P21/CDKN1A was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-P21/CDKN1A Antibody (AAA126971) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Goat Anti-Rabbit IgG and DyLight550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (AAA126971).P21/CDKN1A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-P21/CDKN1A Antibody (AAA126971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (AAA126971).P21/CDKN1A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-P21/CDKN1A Antibody (AAA126971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (AAA126971).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human U20S whole cell lysates,Lane 5: human SIHA whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P21/CDKN1A antigen affinity purified polyclonal antibody (#AAA126971) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for P21/CDKN1A at approximately 21 kDa. The expected band size for P21/CDKN1A is at 21 kDa.)

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P21/CDKN1A, Polyclonal Antibody (Cat# AAA126971)

Full Name

Anti-P21/CDKN1A Antibody Picoband

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of K562 cells using anti-RLN3 antibody (AAA127302).Overlay histogram showing K562 cells stained with AAA127302 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RLN3 Antibody (AAA127302, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of RLN3 using anti-RLN3 antibody (AAA127302).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human THP-1 whole cell lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse tesits tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLN3 antigen affinity purified polyclonal antibody (#AAA127302) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RLN3 at approximately 18-19 kDa. The expected band size for RLN3 is at 15 kDa.)

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RLN3, Polyclonal Antibody (Cat# AAA127302)

Full Name

Anti-RLN3 Antibody Picoband

Gene Names

RLN3; H3; RXN3; ZINS4; insl7

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Hela cells using anti-PSMD13 antibody (AAA127614).Overlay histogram showing Hela cells stained with AAA127614 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD13 Antibody (AAA127614, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of PSMD13 using anti-PSMD13 antibody (AAA127614).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat lung tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse lung tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD13 antigen affinity purified polyclonal antibody (#AAA127614) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSMD13 at approximately 43 kDa. The expected band size for PSMD13 is at 43 kDa.)

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PSMD13, Polyclonal Antibody (Cat# AAA127614)

Full Name

Anti-PSMD13 Antibody Picoband

Gene Names

PSMD13; S11; Rpn9; p40.5; HSPC027

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CYSLTR2 / CYSLT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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CYSLT2 / CYSLTR2, Polyclonal Antibody (Cat# AAA51711)

Full Name

Rabbit Polyclonal to Human CYSLT2 / CYSLTR2

Gene Names

CYSLTR2; HG57; CYSLT2; HPN321; CYSLT2R; KPG_011; hGPCR21; PSEC0146

Reactivity

Gibbon, Gorilla, Human

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of Raji cells using anti-PI3K-gamma/PIK3CG antibody (AAA125609).Overlay histogram showing Raji cells stained with AAA125609 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI3K-gamma/PIK3CG Antibody (AAA125609,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of PI3K-gamma/PIK3CG using anti PI3K-gamma/PIK3CG antibody (AAA125609).PI3K-gamma/PIK3CG was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PI3K-gamma/PIK3CG Antibody (AAA125609) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PI3K-gamma/PIK3CG using anti-PI3K-gamma/PIK3CG antibody (AAA125609).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: human Jurkat whole cell lysatesLane 4: human HepG2 whole cell lysatesLane 5: rat RH35 whole cell lysatesLane 6: rat PC-12 whole cell lysatesLane 7: mouse NIH/3T3 whole cell lysatesLane 8: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PI3K-gamma/PIK3CG antigen affinity purified polyclonal antibody (Catalog # AAA125609) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PI3K-gamma/PIK3CG at approximately 126KD. The expected band size for PI3K-gamma/PIK3CG is at 126KD.)

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PI3K-gamma/PIK3CG, Polyclonal Antibody (Cat# AAA125609)

Full Name

Anti-PI3K-gamma/PIK3CG Antibody

Gene Names

PIK3CG; PI3K; PIK3; PI3CG; PI3Kgamma

Reactivity

Human, Mouse, Rat

Applications

Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-RTCD1/RTCA antibody (AAA125792).Overlay histogram showing CACO-2 cells stained with AAA125792 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCD1/RTCA Antibody (AAA125792, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of RTCD1/RTCA using anti- RTCD1/RTCA antibody (AAA125792).RTCD1/RTCA was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- RTCD1/RTCA Antibody (AAA125792) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of RTCD1/RTCA using anti-RTCD1/RTCA antibody (AAA125792).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: human A431 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RTCD1/RTCA antigen affinity purified polyclonal antibody (Catalog # AAA125792) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RTCD1/RTCA at approximately 39KD. The expected band size for RTCD1/RTCA is at 39KD.)

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RTCD1/RTCA, Polyclonal Antibody (Cat# AAA125792)

Full Name

Anti-RTCD1/RTCA Antibody

Gene Names

RTCA; RPC; RTC1; RTCD1

Reactivity

Human

Applications

Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Biotin) $705/0.1 mg (HRP) $705/0.1 mg (FITC) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $810/0.1 mg (PE) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot of TMP21 in Raji cell lysate with TMP21 antibody at (A) 0.5 and (B) 1 and (C) 2 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of TMP21 in Human Brain cells with TMP21 antibody at 20 ug/ml.)

IHC (Immunohiostchemistry) (Anti-TMED10 / TMP21 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

IHC (Immunohistochemistry) (Anti-TMED10 / TMP21 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

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TMED10 / TMP21, Polyclonal Antibody (Cat# AAA50701)

Full Name

Rabbit Polyclonal to Human TMED10 / TMP21

Gene Names

TMED10; p23; TMP21; S31I125; Tmp-21-I; S31III125; P24(DELTA)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IF (Immunofluorescence) (AAA322387 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA322387 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of NMDAR1 expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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NMDAR1, Polyclonal Antibody (Cat# AAA322387)

Full Name

NMDAR1 Antibody

Gene Names

GRIN1; NR1; MRD8; GluN1; NMDA1; NDHMSD; NDHMSR; NMD-R1; NMDAR1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of NMDAR2B expression in A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA322406 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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NMDAR2B, Polyclonal Antibody (Cat# AAA322406)

Full Name

NMDAR2B Antibody

Gene Names

GRIN2B; NR3; MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HUVEC, using BRD7 Antibody. The lane on the left was treated with blocking peptide.)

IF (Immunofluorescence) (AAA322920 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA322920 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (AAA322920 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

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BRD7, Polyclonal Antibody (Cat# AAA322920)

Full Name

BRD7 Antibody

Gene Names

BRD7; BP75; NAG4; CELTIX1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HEK-293 cells, using NSUN2 antibody.)

IHC (Immunohiostchemistry) (AAA322929 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using NSUN2 antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: hela;)

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NSUN2, Polyclonal Antibody (Cat# AAA322929)

Full Name

NSUN2 Antibody

Gene Names

NSUN2; MISU; MRT5; SAKI; TRM4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of mouse brain tissue, using CCT3 antibody.)

IHC (Immunohistochemistry) (AAA322939 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CCT3, Polyclonal Antibody (Cat# AAA322939)

Full Name

CCT3 Antibody

Gene Names

CCT3; CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from various samples, using LONP1 antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Mouse Myeloma cell;)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using LONP1 antibody.)

IHC (Immunohistochemistry) (AAA322945 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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LONP1, Polyclonal Antibody (Cat# AAA322945)

Full Name

LONP1 Antibody

Gene Names

LONP1; LON; LONP; PIM1; hLON; LonHS; CODASS; PRSS15

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of human lung tissue, using LPCAT1 antibody.)

IHC (Immunohistochemistry) (AAA322860 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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LPCAT1, Polyclonal Antibody (Cat# AAA322860)

Full Name

LPCAT1 Antibody

Gene Names

LPCAT1; AYTL2; lpcat; AGPAT9; PFAAP3; AGPAT10; LPCAT-1; lysoPAFAT

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322638 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of TDE1 using 293 whole cell lysates)

IHC (Immunohistochemistry) (AAA322638 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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TDE1, Polyclonal Antibody (Cat# AAA322638)

Full Name

TDE1 Antibody

Gene Names

SERINC3; TDE; TDE1; AIGP1; TMS-1; DIFF33; SBBI99

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA322350 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322350 staining Hep G2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IF (Immunofluorescence) (AAA322350 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Ataxin 1 expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Ataxin 1, Polyclonal Antibody (Cat# AAA322350)

Full Name

Ataxin 1 Antibody

Gene Names

ATXN1; ATX1; SCA1; D6S504E

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of BAI2 using HepG2 whole cell lysates)

IHC (Immunohistochemistry) (AAA322773 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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BAI2, Polyclonal Antibody (Cat# AAA322773)

Full Name

BAI2 Antibody

Gene Names

ADGRB2; BAI2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322745 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA322745 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of KALRN using HUVEC whole cell lysates)

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KALRN, Polyclonal Antibody (Cat# AAA322745)

Full Name

KALRN Antibody

Gene Names

KALRN; DUO; CHD5; DUET; TRAD; CHDS5; HAPIP; ARHGEF24

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HeLa, using PTEN antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326596 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PTEN, Polyclonal Antibody (Cat# AAA326596)

Full Name

PTEN Antibody

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA326597 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of A431, using PTK2 antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326597 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PTK2, Polyclonal Antibody (Cat# AAA326597)

Full Name

PTK2 Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of heart, using VEGFB antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326614 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA326614 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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VEGFB, Polyclonal Antibody (Cat# AAA326614)

Full Name

VEGFB Antibody

Gene Names

VEGFB; VRF; VEGFL

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Hepg2 whole cell lysates, using XRCC3 Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326616 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326616 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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XRCC3, Polyclonal Antibody (Cat# AAA326616)

Full Name

XRCC3 Antibody

Gene Names

XRCC3; CMM6

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HeLa, using ZYX antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326617 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse muscle, using ZYX Antibody.)

IHC (Immunohiostchemistry) (AAA326617 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse spleen, using ZYX Antibody.)

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ZYX, Polyclonal Antibody (Cat# AAA326617)

Full Name

ZYX Antibody

Gene Names

ZYX; ESP-2; HED-2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Mouse brain lysate, using CASK Antibody. The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using CASK Antibody.)

IHC (Immunohistochemistry) (AAA326719 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CASK, Polyclonal Antibody (Cat# AAA326719)

Full Name

CASK Antibody

Gene Names

CASK; CMG; FGS4; LIN2; TNRC8; hCASK; CAGH39; CAMGUK; MICPCH; MRXSNA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using PSMA1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using PSMA1 Antibody.)

IHC (Immunohistochemistry) (AAA326733 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PSMA1, Polyclonal Antibody (Cat# AAA326733)

Full Name

PSMA1 Antibody

Gene Names

PSMA1; NU; HC2; PROS30; HEL-S-275

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA326742 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Hepg2 whole cell lysates, using RPS3 Antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326742 at 1/100 staining Human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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RPS3, Polyclonal Antibody (Cat# AAA326742)

Full Name

RPS3 Antibody

Gene Names

RPS3; S3

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326423 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of MCF-7, using DDB2 antibody. The lane on the left is treated with the antigen-specific peptide.)

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DDB2, Polyclonal Antibody (Cat# AAA326423)

Full Name

DDB2 Antibody

Gene Names

DDB2; XPE; DDBB; UV-DDB2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA326466 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of K562 cell lysate, using RAB27A Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326466 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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RAB27A, Polyclonal Antibody (Cat# AAA326466)

Full Name

RAB27A Antibody

Gene Names

RAB27A; GS2; RAM; RAB27; HsT18676

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using VIM Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326801 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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VIM, Polyclonal Antibody (Cat# AAA326801)

Full Name

VIM Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326815 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of HepG2, using CFHR1 antibody. The lane on the left is treated with the antigen-specific peptide.)

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CFHR1, Polyclonal Antibody (Cat# AAA326815)

Full Name

CFHR1 Antibody

Gene Names

CFHR1; CFHL; FHR1; HFL1; HFL2; CFHL1; H36-1; H36-2; CFHL1P; CFHR1P

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA326354 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326354 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Jurkat whole cell lysates, using PTH1R Antibody. The lane on the left is treated with the antigen-specific peptide.)

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PTH1R, Polyclonal Antibody (Cat# AAA326354)

Full Name

PTH1R Antibody

Gene Names

PTH1R; PFE; PTHR; PTHR1

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.