At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RBBP5 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using RBBP5 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using RBBP5 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat Intestine using RBBP5 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RBBP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using H2AFZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human stomach using H2AFZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using H2AFZ antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using H2AFZ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using MORF4L1 antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MORF4L1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using GIP antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using GIP antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon using GIP antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using GIP antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GIP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ST3GAL4 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ST3GAL4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BAP1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using CPM antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver injury using CPM antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using CPM antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using CPM Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MYH9 antibody. Western blot was performed from the immunoprecipitate using MYH9 antibody at a dilition of 1:1000.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human stomach using MYH9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using MYH9 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MYH9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human prostate using IFNG antibody (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IFNG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using HK2 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using TFPI antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TFPI antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using HK1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TYK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BRD4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CLEC4D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KLF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using ACSL5 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ACSL5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IL1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver injury using CBLB antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CBLB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen using THBS1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen using THBS1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using THBS1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using THBS1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from U-251 MG cells using THBS1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using THBS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot) (Western blot analysis of extracts of mouse pancreas, using INS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using LAMP1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LAMP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using MOK antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using MOK antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat heart using MOK antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using MOK antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of mouse liver, using MOK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NDP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAT1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using IL1RN antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IL1RN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PARK7 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PARK7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using FOXO3A Rabbit pAb (AAA36654) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using FOXO3A Rabbit pAb (AAA36654) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse kidney using FOXO3A Rabbit pAb (AAA36654) at dilution of 1:50 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon using FOXO3A Rabbit pAb (AAA36654) at dilution of 1:50 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FOXO3A antibody (AAA36654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody. Western blot was performed from the immunoprecipitate using PEBP1 antibody at a dilition of 1:500.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PEBP1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PEBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using ACTA2 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ACTA2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse lung using ENDOG antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ENDOG antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ENDOG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CYCS antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CYCS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CRP antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of rat brain, using CRP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using SPHK1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using SPHK1 Antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SPHK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using HDAC4 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HDAC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human prostate using PIK3C3 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PIK3C3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibody at a dilition of 1:500.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using P4HB antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using P4HB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using EIF4G2 antibody (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using EIF4G2 antibody (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human stomach using EIF4G2 antibody (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver injury using EIF4G2 antibody (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using EIF4G2 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using GATA2 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using GATA2 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GATA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SSTR1 antibody (AAA37147) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PYCARD antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PYCARD antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ATG13 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATG13 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of mouse kidney, using NPHS1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NR0B1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NR0B1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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