At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IF (Immunofluorescence) (AAA326031 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
IHC (Immunohiostchemistry) (AAA326031 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Jurkat lysate using CYR61 antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326041 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of 293T lysate using TNNT2 antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of DCX expression in Rat spleen lysate)
WB (Western Blot) (Western blot analysis of brain lysate using DCX antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326048 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326061 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of T47D lysate using NCF1 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326061 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of PC12 lysate using KDM1A antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326069 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (AAA326469 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of MCF-7, using ACPP antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of Mouse brain lysate, using GRIK2 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326470 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326471 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohiostchemistry) (AAA326471 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of MCF7 cell lysate, using POLI Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326472 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Mouse brain lysate, using APBB1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326473 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of spleen tissue, using CD27 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326476 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using ODC1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts of T47D, using LAMP2 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326483 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from rat heart, using LAMP2 Antibody.)
WB (Western Blot) (Western blot analysis of HepG2 cell lysate, using MSMB Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326484 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Mouse liver tissue lysates, using NDP Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326485 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using MVP Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326496 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using IPO5 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326499 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry) (AAA326534 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of Jurkat, using ICAM2 antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326534 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Mouse liver tissue lysates, using MBP Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326305 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of 293, using TAC1 antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts from mouse liver, rat liver, using TAC1 Antibody.)
IF (Immunofluorescence) (AAA326307 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemistry) (AAA326307 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Mouse liver tissue lysates, using KNG1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326310 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemisry) (AAA326310 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from 293, using KNG1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326310 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts of MCF-7, using SLC22A5 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326315 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of HeLa, using OCT1 antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326319 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohistochemistry) (AAA326319 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of K-562, using S100A8 antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326322 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemistry) (AAA326322 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from COLO cells, using OR10G6 antibody.)
IF (Immunofluorescence) (AAA325652 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohiostchemistry) (Detection of human MCM3 by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-MCM3 (Cat. No. AAA213767) used at a dilution of 1:250. Detection: DAB staining using anti-rabbit IHC antibody (Cat. No. at a dilution of 1:100.)
IHC (Immunohistochemistry) (Detection of mouse MCM3 by immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-MCM3 (Cat. No. AAA213767) used at a dilution of 1:250. Detection: DAB)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/15.)
Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): MCF7 and A172 cell Amount of lysate: 40ug per lane Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)
FCM/FACS (Flow Cytometry) (TAF2 Antibody (C-Term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (TAF2 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF2 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of lysates from HT-29, Jurkat, KG-1, PC-3 cell line (from left to right), using TAF2 Antibody (C-Term). AAA288011 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry) (MLF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry) (MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of MLF1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab.)
WB (Western Blot) (MLF1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MLF1 antibody detected the MLF1 protein (arrow).)
WB (Western Blot) (Western blot analysis of MLF1 (arrow) using rabbit polyclonal MLF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.)
IHC (Immunohiostchemistry) (5NT1A Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the 5NT1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of 5NT1A Antibody (N-term) in mouse heart tissue lysates (35ug/lane). 5NT1A (arrow) was detected using the purified Pab.)
WB (Western Blot) (C10orf63 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the C10orf63 antibody detected the C10orf63 protein (arrow).)
WB (Western Blot) (C10orf63 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the C10orf63 antibody detected the C10orf63 protein (arrow).)
FCM/FACS (Flow Cytometry) (CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.)
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot) (PAPD5 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the PAPD5 Antibody detected the PAPD5 protein (arrow).)
WB (Western Blot) (RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
FCM/FACS (Flow Cytometry) (RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
FCM/FACS (Flow Cytometry) (MeCP2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (C413). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.)
WB (Western Blot) (Mouse CD31 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the MouseCD31 antibody detected the MouseCD31 protein (arrow).)
WB (Western Blot) (Mouse CD31 Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the MouseCD31 antibody detected the Mouse CD31 protein (arrow).)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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