At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 7100-7150 of 98831 product results
WB (Western Blot) (PAPD5 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the PAPD5 Antibody detected the PAPD5 protein (arrow).)
IHC (Immunohiostchemistry) (CDR2 Antibody (C-term) (AAA289107)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDR2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (CDR2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CDR2 antibody detected the CDR2 protein (arrow).)
FCM/FACS (Flow Cytometry) (MeCP2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (C413). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.)
FCM/FACS (Flow Cytometry) (KIF11 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of KIF11 Antibody (N-term) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.)
WB (Western Blot) (ANGPT1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ANGPT1 antibody detected the ANGPT1 protein (arrow).)
WB (Western Blot) (Anti-ANGPT1 Antibody (C-term)at 1:2000 dilution + mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot) (Mouse CD31 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the MouseCD31 antibody detected the MouseCD31 protein (arrow).)
WB (Western Blot) (Mouse CD31 Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the MouseCD31 antibody detected the Mouse CD31 protein (arrow).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/15.)
Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): MCF7 and A172 cell Amount of lysate: 40ug per lane Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/60.)
Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): Hela and A549 cell Amount of lysate: 30ug per lane Primary antibody: 1/1200 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)
SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse kidney tissue, Mouse heart tissue, Primary antibody: AAA239214(NDUFS4 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA239214(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA239214(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))
SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: 293T cells, A549 cells, human testis tissue, mouse thymus tissue, Primary antibody: AAA239171(MRPL28 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA239171(MRPL28 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA239171(MRPL28 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241508(SLC22A2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241508(SLC22A2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
WB (Western Blot) (WB Suggested Anti-ARG2 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysateARG2 is supported by BioGPS gene expression data to be expressed in Jurkat)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using RHAG Polyclonal Antibody at dilution of 1:130(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using RHAG Polyclonal Antibody at dilution of 1:130(×200))
WB (Western Blot) (Western blot analysis of 293T K562 and HepG2 cell lysates using RHAG Polyclonal Antibody at dilution of 1:1600)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse testis cells using ASZ1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of Rat testis cells using ASZ1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse testis using ASZ1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat testis using ASZ1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using SEMA4A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using SEMA4A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of HT-29 cells using SEMA4A Polyclonal Antibody at1:3000 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using MGRN1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of Rat heart using MGRN1 Polyclonal Antibody at 1:1000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using TRRAP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using TRRAP Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot) (Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4 degree over night)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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