Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue using PI 3-kinase p85? Polyclonal Antibody at dilution of 1:200.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus tissue using PI 3-kinase p85? Polyclonal Antibody at dilution of 1:200.)

WB (Western Blot) (Western Blot analysis of various cells using PI 3-kinase p85? Polyclonal Antibody at dilution of 1:2000.)

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PI 3-kinase p85alpha, Polyclonal Antibody (Cat# AAA171686)

Full Name

PI 3-kinase p85alpha Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using BAG3 Polyclonal Antibody at dilution of 1:35(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using BAG3 Polyclonal Antibody at dilution of 1:35(×200))

WB (Western Blot) (Western blot analysis of Mouse heart tissue lysate using BAG3 Polyclonal Antibody at dilution of 1:250)

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BAG3, Polyclonal Antibody (Cat# AAA175045)

Full Name

BAG3 Polyclonal Antibody

Gene Names

BAG3; BIS; MFM6; BAG-3; CAIR-1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $245/0.06 mL $350/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,240/5x0.2 mL

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WB (Western Blot) (Host: RabbitTarget Name: GSPT1Sample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-GSPT1 AntibodyTitration: 1.0 ug/mlPositive Control: HepG2 Whole CellGSPT1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)

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GSPT1, Polyclonal Antibody (Cat# AAA201309)

Full Name

GSPT1 Antibody - N-terminal region

Gene Names

GSPT1; GST1; ETF3A; eRF3a; 551G9.2

Reactivity

Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Rat, Cow, Dog, Pig, Rabbit

Applications

Western Blot

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded rat brain using GAP43 Polyclonal Antibody at dilution of 1:300.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded mouse brain using GAP43 Polyclonal Antibody at dilution of 1:300.)

WB (Western Blot) (Western Blot analysis of various samples using GAP43 Polyclonal Antibody at dilution of 1:2000.)

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GAP43, Polyclonal Antibody (Cat# AAA174372)

Full Name

GAP43 Polyclonal Antibody

Gene Names

GAP43; B-50; PP46

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$255/0.06 mL $355/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,255/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ADORA2B Polyclonal Antibody at dilution of 1:30(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ADORA2B Polyclonal Antibody at dilution of 1:30(×200))

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ADORA2B, Polyclonal Antibody (Cat# AAA175709)

Full Name

ADORA2B Polyclonal Antibody

Gene Names

ADORA2B; ADORA2

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TMED10 Polyclonal Antibody at dilution of 1:65(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TMED10 Polyclonal Antibody at dilution of 1:65(×200))

WB (Western Blot) (Western blot analysis of NIH/3T3 cell Mouse Pancreas tissue Rat lung tissue Mouse liver tissue Hela Raji cell lysates using TMED10 Polyclonal Antibody at dilution of 1:900)

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TMED10, Polyclonal Antibody (Cat# AAA175608)

Full Name

TMED10 Polyclonal Antibody

Gene Names

TMED10; p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA)

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PPP2R5D Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using PPP2R5D Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse kidney using PPP2R5D Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using PPP2R5D Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using PPP2R5D Polyclonal Antibody at dilution of 1:100 (40x lens).)

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PPP2R5D, Polyclonal Antibody (Cat# AAA177289)

Full Name

PPP2R5D Polyclonal Antibody

Gene Names

PPP2R5D; B56D

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RARB Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using RARB Polyclonal Antibody at dilution of 1:100 (40x lens).)

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RARB, Polyclonal Antibody (Cat# AAA177330)

Full Name

RARB Polyclonal Antibody

Gene Names

RARB; HAP; RRB2; NR1B2

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using SHANK3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human brain using SHANK3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Mouse brain using SHANK3 Polyclonal Antibody at 1:1000 dilution.)

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SHANK3, Polyclonal Antibody (Cat# AAA179286)

Full Name

SHANK3 Polyclonal Antibody

Gene Names

SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using BRPF1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using BRPF1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using BRPF1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Mouse testis using BRPF1 Polyclonal Antibody at1:1000 dilution.)

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BRPF1, Polyclonal Antibody (Cat# AAA179084)

Full Name

BRPF1 Polyclonal Antibody

Gene Names

BRPF1; BR140

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from wild type(WT) and HER2/ErbB2 knockout (KO) HeLa cells using HER2/ErbB2 Polyclonal Antibody at 1:500 dilution.)

WB (Western Blot) (Western blot analysis of MCF7 using HER2/ErbB2 Polyclonal Antibody at 1:500 dilution.)

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HER2/ErbB2, Polyclonal Antibody (Cat# AAA179592)

Full Name

(KO Validated) HER2/ErbB2 Polyclonal Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Western Blot

Purity

Affinity purification

Pricing

$315/0.06 mL $450/0.12 mL $685/0.2 mL $1,335/2x0.2 mL $1,945/3x0.2 mL $2,525/4x0.2 mL $3,075/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of THP-1 cells using anti-Azurocidin/AZU1 antibody (AAA126416).Overlay histogram showing THP-1 cells stained with AAA126416 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Azurocidin/AZU1 Antibody (AAA126416, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Azurocidin/AZU1 using anti-Azurocidin/AZU1 antibody (AAA126416).Azurocidin/AZU1 was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Azurocidin/AZU1 Antibody (AAA126416) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Azurocidin/AZU1 using anti-Azurocidin/AZU1 antibody (AAA126416).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: mouse spleen tissue lysates,Lane 4: mouse thymus tissue lysates,Lane 5: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Azurocidin/AZU1 antigen affinity purified polyclonal antibody (#AAA126416) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Azurocidin/AZU1 at approximately 37 kDa. The expected band size for Azurocidin/AZU1 is at 37 kDa.)

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Azurocidin/AZU1, Polyclonal Antibody (Cat# AAA126416)

Full Name

Anti-Azurocidin/AZU1 Antibody Picoband

Gene Names

AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of SiHa cells using anti-FADS1 antibody (AAA126233).Overlay histogram showing SiHa cells stained with AAA126233 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FADS1 Antibody (AAA126233, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of FADS1 using anti-FADS1 antibody (AAA126233).FADS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FADS1 Antibody (AAA126233) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of FADS1 using anti-FADS1 antibody (AAA126233).FADS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FADS1 Antibody (AAA126233) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of FADS1 using anti-FADS1 antibody (AAA126233).FADS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FADS1 Antibody (AAA126233) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of FADS1 using anti-FADS1 antibody (AAA126233).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified polyclonal antibody (#AAA126233) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.)

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FADS1, Polyclonal Antibody (Cat# AAA126233)

Full Name

Anti-FADS1 Antibody Picoband

Gene Names

FADS1; D5D; TU12; FADS6; FADSD5; LLCDL1

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of HL-60 cells using anti-TOX antibody (AAA126522).Overlay histogram showing HL-60 cells stained with AAA126522 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOX Antibody (AAA126522, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of TOX using anti-TOX antibody (AAA126522).TOX was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TOX Antibody (AAA126522) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of TOX using anti-TOX antibody (AAA126522).TOX was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TOX Antibody (AAA126522) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of TOX using anti-TOX antibody (AAA126522).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human Ramos whole cell lysates,Lane 3: human HL-60 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX antigen affinity purified polyclonal antibody (#AAA126522) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TOX at approximately 57 kDa. The expected band size for TOX is at 57 kDa.)

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TOX, Polyclonal Antibody (Cat# AAA126522)

Full Name

Anti-TOX Antibody Picoband

Gene Names

TOX; TOX1

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of h. PBMC cells using anti-CCR2 antibody (AAA125520).Overlay histogram showing h. PBMC cells stained with AAA125520 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR2 Antibody (AAA125520, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of CCR2 using anti-CCR2 antibody (AAA125520).CCR2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCR2 Antibody (AAA125520) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of CCR2 using anti-CCR2 antibody (AAA125520).CCR2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCR2 Antibody (AAA125520) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CCR2 using anti-CCR2 antibody (AAA125520).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # AAA125520) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR2 at approximately 42KD. The expected band size for CCR2 is at 42KD.)

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CCR2, Polyclonal Antibody (Cat# AAA125520)

Full Name

Anti-CCR2 Antibody

Gene Names

CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2

Reactivity

Human

Applications

Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U87 cells using anti-Epac1/RAPGEF3 antibody (AAA125656).Overlay histogram showing U87 cells stained with AAA125656 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epac1/RAPGEF3 Antibody (AAA125656, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA125656).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human placenta tissue lysatesLane 3: monkey kidney tissue lysatesLane 4: rat kidney tissue lysatesLane 5: rat lung tissue lysatesLane 6: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Epac1/RAPGEF3 antigen affinity purified polyclonal antibody (Catalog # AAA125656) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Epac1/RAPGEF3 at approximately 104KD. The expected band size for Epac1/RAPGEF3 is at 104KD.)

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Epac1/RAPGEF3, Polyclonal Antibody (Cat# AAA125656)

Full Name

Anti-Epac1/RAPGEF3 Antibody

Gene Names

RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI

Reactivity

Human, Mouse, Rat, Monkey

Applications

Direct ELISA, Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Raji cells using anti-Nir2/PITPNM1 antibody (AAA127503).Overlay histogram showing Raji cells stained with AAA127503 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nir2/PITPNM1 Antibody (AAA127503, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of Nir2/PITPNM1 using anti-Nir2/PITPNM1 antibody (AAA127503).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nir2/PITPNM1 antigen affinity purified polyclonal antibody (#AAA127503) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nir2/PITPNM1 at approximately 150 kDa. The expected band size for Nir2/PITPNM1 is at 135 kDa.)

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Nir2/PITPNM1, Polyclonal Antibody (Cat# AAA127503)

Full Name

Anti-Nir2/PITPNM1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of RT4 cells using anti-PTGR1 antibody (AAA127533).Overlay histogram showing RT4 cells stained with AAA127533 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGR1 Antibody (AAA127533, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of PTGR1 using anti-PTGR1 antibody (AAA127533).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: human SiHa whole cell lysates,Lane 6: human Hacat whole cell lysates,Lane 7: human Caco-2 whole cell lysates,Lane 8: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR1 antigen affinity purified polyclonal antibody (#AAA127533) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTGR1 at approximately 36 kDa. The expected band size for PTGR1 is at 36 kDa.)

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PTGR1, Polyclonal Antibody (Cat# AAA127533)

Full Name

Anti-PTGR1 Antibody Picoband

Gene Names

PTGR1; PGR1; ZADH3; LTB4DH

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PI4K2B antibody (AAA127692).Overlay histogram showing MCF-7 cells stained with AAA127692 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI4K2B Antibody (AAA127692, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of PI4K2B using anti-PI4K2B antibody (AAA127692).PI4K2B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PI4K2B Antibody (AAA127692) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of PI4K2B using anti-PI4K2B antibody (AAA127692).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI4K2B antigen affinity purified polyclonal antibody (#AAA127692) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PI4K2B at approximately 50 kDa. The expected band size for PI4K2B is at 55,50 kDa.)

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PI4K2B, Polyclonal Antibody (Cat# AAA127692)

Full Name

Anti-PI4K2B Antibody Picoband

Gene Names

PI4K2B; PIK42B; PI4KIIB

Reactivity

Human, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot of VISA in rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of VISA in Mouse Brain cells with VISA antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Anti-MAVS antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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VISA / MAVS, Polyclonal Antibody (Cat# AAA50805)

Full Name

Rabbit Polyclonal to Human VISA / MAVS

Gene Names

MAVS; IPS1; VISA; IPS-1; CARDIF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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WB (Western Blot) (Western blot of anti-ACE2 (SARS Receptor) Antibody (Center) in HL60 cell line lysates (35 ug/lane). ACE2 (arrow) was detected using the purified Pab.)

FCM/FACS (Flow Cytometry) (Flow cytometry of HL-60 cells using ACE2 (SARS Receptor) Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

IHC (Immunohiostchemistry) (Anti-ACE-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-ACE-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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ACE2 / ACE-2, Polyclonal Antibody (Cat# AAA51252)

Full Name

Rabbit Polyclonal to Human ACE2 / ACE-2

Gene Names

ACE2; ACEH

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

Ammonium sulfate precipitation

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

WB (Western Blot) (Western Blot: Sample: Recombinant IL1RA, Rabbit.)

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Interleukin 1 Receptor Antagonist, Polyclonal Antibody (Cat# AAA142173)

Full Name

Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA)

Gene Names

IL1RN; IL1RA; icIL-1ra

Reactivity

Rabbit

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography

Pricing

$215/0.1 mL $280/0.2 mL $435/0.5 mL $525/1 mL $1,420/5 mL $2,765/2x5 mL $4,035/3x5 mL $5,240/4x5 mL $6,405/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Fibronectin Leucine Rich Transmembrane Protein 3, Polyclonal Antibody (Cat# AAA143073)

Full Name

Polyclonal Antibody to Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3)

Gene Names

FLRT3; HH21

Reactivity

Human, Mouse, Rat, Pig

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Hivep3 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using Hivep3 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of Rat brain, using Hivep3 antibody.)

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Hivep3, Polyclonal Antibody (Cat# AAA314695)

Full Name

Hivep3 Rabbit Polyclonal Antibody

Gene Names

Hivep3; Rc; Krc; KBP1; Shn3; Zas3; KBP-1; AI848000; A130075N07; Schnurri-3; 2900056N03Rik; E030045D18Rik

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-heart, antibody was diluted at 1:100)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human-heart, antibody was diluted at 1:100)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-skeletal-muscle, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-skeletal-muscle, antibody was diluted at 1:100)

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Cytoglobin, Polyclonal Antibody (Cat# AAA318777)

Full Name

Cytoglobin Rabbit Polyclonal Antibody

Gene Names

CYGB; HGB; STAP

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of Integrin alphaV antibody in paraffin-embedded human brain tissue.)

WB (Western Blot) (Western blot analysis of lysate from HeLa cells, using Integrin alphaV antibody.)

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Integrin alphaV, Polyclonal Antibody (Cat# AAA318458)

Full Name

Integrin alphaV Antibody

Gene Names

ITGAV; CD51; MSK8; VNRA; VTNR

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MUNC-18a (Phospho-Ser313) Antibody)

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MUNC-18a, Polyclonal Antibody (Cat# AAA318360)

Full Name

MUNC-18a (Phospho-Ser313) Antibody

Gene Names

STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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IF (Immunofluorescence) (AAA322476 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of ApoOL using K562 whole cell lysates)

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ApoOL, Polyclonal Antibody (Cat# AAA322476)

Full Name

ApoOL Antibody

Gene Names

APOOL; Mic27; CXorf33; FAM121A; UNQ8193

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322711 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of TCF2 using 293 whole cell lysates)

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TCF2, Polyclonal Antibody (Cat# AAA322711)

Full Name

TCF2 Antibody

Gene Names

HNF1B; FJHN; HNF2; LFB3; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; HNF-1B; HNF1beta; HNF-1-beta

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HT29 cells treated with nocodazole, using Phospho-Myt1 (Ser83) Antibody.)

IHC (Immunohistochemistry) (AAA321353 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Myt1, Polyclonal Antibody (Cat# AAA321353)

Full Name

Phospho-Myt1 (Ser83) Antibody

Gene Names

PKMYT1; MYT1; PPP1R126

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA326556 staining SW626 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA326556 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of HeLa, using CTNNB1 antibody. The lane on the left is treated with the antigen-specific peptide.)

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Catenin-beta, Polyclonal Antibody (Cat# AAA326556)

Full Name

Catenin-beta Antibody

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA326557 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using DNMT3A Antibody. The lane on the left is treated with the antigen-specific peptide.)

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DNMT3A, Polyclonal Antibody (Cat# AAA326557)

Full Name

DNMT3A Antibody

Gene Names

DNMT3A; TBRS; DNMT3A2; M.HsaIIIA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA326560 at 1/100 staining Human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of HeLa Jurkat, using EEF2 antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326560 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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EEF2, Polyclonal Antibody (Cat# AAA326560)

Full Name

EEF2 Antibody

Gene Names

EEF2; EF2; EF-2; EEF-2; SCA26

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA326566 at 1/100 staining Human Head and neck cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of mouse heart tissue, using GNA15 antibody. The lane on the left is treated with the antigen-specific peptide.)

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GNA15, Polyclonal Antibody (Cat# AAA326566)

Full Name

GNA15 Antibody

Gene Names

GNA15; GNA16

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of Raji, using HCK antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326568 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HCK, Polyclonal Antibody (Cat# AAA326568)

Full Name

HCK Antibody

Gene Names

HCK; JTK9; p59Hck; p61Hck

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA326572 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using IKBKB Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326572 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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IKBKB, Polyclonal Antibody (Cat# AAA326572)

Full Name

IKBKB Antibody

Gene Names

IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA326578 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Mouse brain lysate, using LYN Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326578 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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LYN, Polyclonal Antibody (Cat# AAA326578)

Full Name

LYN Antibody

Gene Names

LYN; JTK8; p53Lyn; p56Lyn

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HeLa, using MKI67 antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326579 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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MKI67, Polyclonal Antibody (Cat# AAA326579)

Full Name

MKI67 Antibody

Gene Names

MKI67; KIA; MIB-; MIB-1; PPP1R105

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA326580 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of K562, using NCF4 antibody. The lane on the left is treated with the antigen-specific peptide.)

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NCF4, Polyclonal Antibody (Cat# AAA326580)

Full Name

NCF4 Antibody

Gene Names

NCF4; NCF; CGD3; P40PHOX; SH3PXD4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Mouse brain lysate, using NGFR Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326581 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326581 at 1/100 staining human Glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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NGFR, Polyclonal Antibody (Cat# AAA326581)

Full Name

NGFR Antibody

Gene Names

NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HepG2, using PDGFRA antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326587 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PDGFRA, Polyclonal Antibody (Cat# AAA326587)

Full Name

PDGFRA Antibody

Gene Names

PDGFRA; CD140A; PDGFR2; PDGFR-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of A431, using PGR antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326588 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326588 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PGR, Polyclonal Antibody (Cat# AAA326588)

Full Name

PGR Antibody

Gene Names

PGR; PR; NR3C3

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA326593 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using PRKCE Antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326593 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PRKCE, Polyclonal Antibody (Cat# AAA326593)

Full Name

PRKCE Antibody

Gene Names

PRKCE; PKCE; nPKC-epsilon

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of HepG2, using THBS1 antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326607 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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THBS1, Polyclonal Antibody (Cat# AAA326607)

Full Name

THBS1 Antibody

Gene Names

THBS1; TSP; THBS; TSP1; TSP-1; THBS-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using CUL1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326618 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326618 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CUL1, Polyclonal Antibody (Cat# AAA326618)

Full Name

CUL1 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA326619 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326619 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of COS7 cell lysate, using TP63 Antibody. The lane on the left is treated with the antigen-specific peptide.)

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TP63, Polyclonal Antibody (Cat# AAA326619)

Full Name

TP63 Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA326623 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using NR1H3 Antibody.)

WB (Western Blot) (Western blot analysis of extracts of MCF7, using NR1H3 antibody. The lane on the left is treated with the antigen-specific peptide.)

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NR1H3, Polyclonal Antibody (Cat# AAA326623)

Full Name

NR1H3 Antibody

Gene Names

NR1H3; LXRA; LXR-a; RLD-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA326626 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326626 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of hela, using MALT1 antibody. The lane on the left is treated with the antigen-specific peptide.)

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MALT1, Polyclonal Antibody (Cat# AAA326626)

Full Name

MALT1 Antibody

Gene Names

MALT1; MLT; MLT1; IMD12; PCASP1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA326627 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326627 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Jurkat whole cell lysates, using CHEK2 Antibody. The lane on the left is treated with the antigen-specific peptide.)

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CHEK2, Polyclonal Antibody (Cat# AAA326627)

Full Name

CHEK2 Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326630 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Mouse heart lysate, using SCCPDH Antibody. The lane on the left is treated with the antigen-specific peptide.)

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SCCPDH, Polyclonal Antibody (Cat# AAA326630)

Full Name

SCCPDH Antibody

Gene Names

SCCPDH; NET11; CGI-49

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.