Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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WB (Western Blot) (AAA61675 (1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) (AAA61675 (1ug/ml) staining of HeLa (A) and Jurkat (B) nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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Lamin B1, Polyclonal Antibody (Cat# AAA61675)

Full Name

Goat anti-Lamin B1 (aa526-537) Antibody

Gene Names

LMNB1; LMN; ADLD; LMN2; LMNB

Reactivity

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using PKM Polyclonal Antibody at dilution of 1/25)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using PKM Polyclonal Antibody at dilution of 1/25)

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PKM, Polyclonal Antibody (Cat# AAA173360)

Full Name

PKM Polyclonal Antibody

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse brain tissue using Synuclein-alpha Polyclonal Antibody at dilution of 1:200.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human kidney cancer tissue using Synuclein-alpha Polyclonal Antibody at dilution of 1:200.)

WB (Western Blot) (Western Blot analysis of Hela cells using Synuclein-alpha Polyclonal Antibody at dilution of 1:20000.)

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Synuclein-alpha, Polyclonal Antibody (Cat# AAA173814)

Full Name

Synuclein-alpha Polyclonal Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Anti-5HT4 Receptor antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry) (Anti-HTR4 / 5-HT4 Receptor antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-HTR4 / 5-HT4 Receptor antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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HTR4 / 5-HT4 Receptor, Polyclonal Antibody (Cat# AAA51219)

Full Name

Rabbit Polyclonal to Human HTR4 / 5-HT4 Receptor

Gene Names

HTR4; 5-HT4; 5-HT4R

Reactivity

Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Pig, Rabbit
Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$645/0.05 mg $1,250/2x0.05 mg $1,830/3x0.05 mg $2,375/4x0.05 mg $2,670/5x0.05 mg

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DB (Dot Blot) (Histone H3 dimethyl Lys36 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys36 antibody for dimethyl Lys36 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with Histone H3 dimethyl Lys36 antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9, 14, 18. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: dimethyl Lys14. Lane 10: monomethyl Lys18. Lane 11: dimethyl Lys18. Lane 12: trimethyl Lys18. Bottom panel: Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.)

WB (Western Blot) (Histone H3 dimethyl Lys36 antibody tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with Histone H3 dimethyl Lys36 antibody at a dilution of 1 ug/ml.)

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Histone H3K36me2, Polyclonal Antibody (Cat# AAA59943)

Full Name

Histone H3K36me2 antibody (pAb)

Reactivity

Budding Yeast, Human, Wide Range Predicted

Applications

Western Blot, Dot Blot

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $705/0.1 mg $1,375/2x0.1 mg $2,005/3x0.1 mg $2,605/4x0.1 mg $3,015/5x0.1 mg

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WB (Western Blot) (ETO / RUNX1T1 antibody (pAb) tested by Western Blot: K-562 nuclear extract (20 ug per lane) probed with the ETO / RUNX1T1 antibody (pAb) at a dilution of 1:500.)

WB (Western Blot) (ETO / RUNX1T1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ETO / RUNX1T1 antibody was used to immunoprecipitate ETO / RUNX1T1 from 250 ug of K562 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ETO / RUNX1T1 antibody at a dilution of 1:500.)

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ETO/RUNX1T1, Polyclonal Antibody (Cat# AAA59980)

Full Name

ETO/RUNX1T1 antibody (pAb)

Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Purity

Affinity Purified

Pricing

$250/0.01 mL $705/0.1 mL $1,375/2x0.1 mL $2,005/3x0.1 mL $2,605/4x0.1 mL $3,015/5x0.1 mL

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DB (Dot Blot) (Histone H3K4me3 antibody (pAb) tested by dot blot analysis. Dot blot was used to confirm specificity of Histone H3K4me3 antibody. Peptides corresponding to regions around major sites of histone H3 methylation (lysine 4, lysine 9, lysine 27) were spotted onto PVDF and probed with antibody at a dilution of 1:500. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified H3K4. Lane 2: H3K4me1. Lane 3: H3K4me2. Lane 4: H3K4me3. Lane 5: unmodified H3K9. Lane 6: H3K9me1. Lane 7: H3K9me2. Lane 8: H3K9me3. Lane 9: unmodified H3K79. Lane 10: H3K79me1. Lane 11: H3K79me2. Lane 12: H3K79me3. Bottom panel - Lane 1: unmodified H3K23. Lane 2: H3K23me1. Lane 3: H3K23me3. Lane 4: H3K23me3. Lane 5: unmodified H3K27. Lane 6: H3K27me1. Lane 7: H3K27me2. Lane 8: H3K27me3. Lane 9: unmodified H3K36. Lane 10: H3K36me1. Lane 11: H3K36me2. Lane 12: H3K36me3.)

WB (Western Blot) (Histone H3K4me3 antibody (pAb) tested by Western blot. HeLa nuclear extract (20 ug) was probed with Histone H3K4me3 antibody (pAb) at a 1:1000 dilution. Note: For optimal results, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. It is also recommended to include 0.05% Tween 20 in all blocking solutions to reduce background. Individual optimization may be required.)

IF (Immunofluorescence) (Histone H3 trimethyl Lys4 antibody tested by immunofluorescence. HeLa cells stained at 1:1,000 with Histone H3 trimethyl Lys4 antibody. Top left: Histone H3 trimethyl Lys4 antibody.Top right: Stained with DAPI. Bottom left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom right: Merge of all 3 images.)

ChIP (Chromatin Immunoprecipitation) (Histone H3 trimethyl Lys4 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 2 million HL-60 cells and 3 ul of Histone H3 trimethyl Lys4 antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)

ChIP (Chromatin Immunoprecipitation) (Histone H3K4me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug chromatin from human acute myelocytic leukemia cells and 3 ul of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12.1 million sequence tags were mapped to identify H3K4me3 binding sites on chromosome 12.)

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Histone H3K4me3, Polyclonal Antibody (Cat# AAA59808)

Full Name

Histone H3K4me3 antibody (pAb)

Reactivity

Budding Yeast, Human, Mouse, Wide Range Predicted

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Dot Blot, Chromatin immunoprecipitation, Chromatin immunoprecipitation

Pricing

$250/0.01 mL $455/0.05 mL $725/0.1 mL $1,415/2x0.1 mL $2,070/3x0.1 mL $2,680/4x0.1 mL $3,100/5x0.1 mL

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WB (Western Blot) (Antibody (0.01 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-Perilipin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

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PLIN1 / Perilipin, Polyclonal Antibody (Cat# AAA50916)

Full Name

Goat Polyclonal to Human PLIN1 / Perilipin

Gene Names

PLIN1; PERI; PLIN; FPLD4

Reactivity

Human, Monkey

Applications

Western Blot, Immunohistochemistry

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0. 01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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TCF3/ITF1, Polyclonal Antibody (Cat# AAA61087)

Full Name

Goat anti-TCF3/ITF1 Antibody

Gene Names

TCF3; E2A; E47; AGM8; ITF1; VDIR; TCF-3; bHLHb21

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot) (Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

WB (Western Blot) (HEK293 overexpressing GRAP2 (RC206546) and probed (mock transfection in first lane), tested by Origene.)

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GRAP2/GRID/Grf40, Polyclonal Antibody (Cat# AAA61110)

Full Name

Goat anti-GRAP2/GRID/Grf40 Antibody

Gene Names

GRAP2; P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

WB (Western Blot) ((1ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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Integrin alpha 11, Polyclonal Antibody (Cat# AAA61564)

Full Name

Goat anti-Integrin alpha 11 (mouse) Antibody

Gene Names

Itga11; 4732459H24Rik

Reactivity

Tested: Mouse; Expected from sequence similarity: Mouse, Rat

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohiostchemistry) (Anti-p63 Picoband antibody, AAA46027-2.JPGIHC(P): Human Oesophagus Squama Cancer Tissue)

Application Data (Anti-p63 Picoband antibody, AAA46027-1.jpgAll lanes: Anti P63 (AAA46027) at 0.5ug/mlWB: Recombinant Human P63 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)

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Tumor protein 63, Polyclonal Antibody (Cat# AAA46027)

Full Name

Anti-p63 Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human. No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

WB (Western Blot) (Western blot analysis of extracts of Mouse lung, using PIKFYVE antibody (AAA37642) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

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PIKFYVE, Polyclonal Antibody (Cat# AAA37642)

Full Name

PIKFYVE Polyclonal Antibody

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of BCLAF1 in mouse brain tissue with BCLAF1 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of BCLAF1 in human brain tissue lysate with BCLAF1 antibody at 1 μg/ml.)

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BCLAF1, Polyclonal Antibody (Cat# AAA40848)

Full Name

BCLAF1 Antibody

Gene Names

BCLAF1; BTF; bK211L9.1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) ((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

WB (Western Blot) ((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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CLPP, Polyclonal Antibody (Cat# AAA60885)

Full Name

Goat anti-CLPP Antibody

Gene Names

CLPP; DFNB81; PRLTS3

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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FCM/FACS (Flow Cytometry) (AAA60905 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5%Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

WB (Western Blot)

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VDR, Polyclonal Antibody (Cat# AAA60905)

Full Name

Goat anti-VDR Antibody

Gene Names

VDR; NR1I1; PPP1R163

Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

WB (Western Blot) (Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

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Annexin A2, Polyclonal Antibody (Cat# AAA60930)

Full Name

Goat anti-Annexin A2 Antibody

Gene Names

ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270

Reactivity

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IF (Immunofluorescence) (Affinity Purified Chicken anti-Campylobacter Jejuni (202)-Reacts with Hippurate hydrolase.)

IF (Immunofluorescence) (Affinity Purified Chicken anti-Campylobacter Jejuni (202)-Reacts with Hippurate hydrolase.)

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Campylobacter Jejuni, Polyclonal Antibody (Cat# AAA76869)

Full Name

Anti-Campylobacter Jejuni Antibody-Affinity Purified

Reactivity

Campylobacter jejuni

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Antigen Affinity Purified

Pricing

$570/1 mg $1,100/2x1 mg $1,605/3x1 mg $2,085/4x1 mg $2,360/5x1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using Complement Factor B Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse lung using Complement Factor B Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse heart using Complement Factor B Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human mammary cancer using Complement Factor B Polyclonal Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using Complement Factor B Polyclonal Antibody at dilution of 1:1000.)

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Complement Factor B, Polyclonal Antibody (Cat# AAA177459)

Full Name

Complement Factor B Polyclonal Antibody

Gene Names

CFB; BF; FB; BFD; GBG; CFAB; PBF2; AHUS4; FBI12; H2-Bf

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human kidney using AAA117888 at dilution 1:100)

WB (Western Blot) (Western blotAll lanes: IFNA14 antibody at 2ug/mlLane 1: A549 whole cell lysateLane 2: Mouse heart tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 23 kDaObserved band size: 23 kDa)

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Interferon alpha-14, Polyclonal Antibody (Cat# AAA117888)

Full Name

Rabbit anti-human Interferon alpha-14 polyclonal Antibody

Gene Names

IFNA14; LEIF2H; IFN-alphaH

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

>95%, Protein G purified

Pricing

$235/0.05 mg $345/0.1 mg $665/2x0.1 mg $965/3x0.1 mg $1,255/4x0.1 mg $1,490/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using LGALS9 Polyclonal Antibody at dilution of 1:30(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using LGALS9 Polyclonal Antibody at dilution of 1:30(×200))

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LGALS9, Polyclonal Antibody (Cat# AAA176885)

Full Name

LGALS9 Polyclonal Antibody

Gene Names

LGALS9; HUAT; LGALS9A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $245/0.06 mL $350/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,240/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using NUDT6 Polyclonal Antibody at dilution of 1:30(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using NUDT6 Polyclonal Antibody at dilution of 1:30(×200))

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NUDT6, Polyclonal Antibody (Cat# AAA176195)

Full Name

NUDT6 Polyclonal Antibody

Gene Names

NUDT6; GFG1; GFG-1; ASFGF2; FGF-AS; FGF2AS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PERM1 antibody (AAA127869).Overlay histogram showing MCF-7 cells stained with AAA127869 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PERM1 Antibody (AAA127869, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of PERM1 using anti-PERM1 antibody (AAA127869) and anti-Beta Tubulin antibody (M01857-3).PERM1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PERM1 Antibody (AAA127869) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG (B) and DyLight550 Conjugated Goat Anti-Mouse IgG (BA1133)(D) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI(C). Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of PERM1 using anti-PERM1 antibody (AAA127869).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERM1 antigen affinity purified polyclonal antibody (#AAA127869) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PERM1 at approximately 75 kDa. The expected band size for PERM1 is at 85 kDa.)

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PERM1, Polyclonal Antibody (Cat# AAA127869)

Full Name

Anti-PERM1 Antibody Picoband

Gene Names

PERM1; C1orf170

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cell using UPF1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using UPF1 antibody.)

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UPF1, Polyclonal Antibody (Cat# AAA303563)

Full Name

UPF1 Antibody

Gene Names

UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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Arrestin 1, Polyclonal Antibody (Cat# AAA302246)

Full Name

Arrestin 1 (Phospho-Ser412) Antibody

Gene Names

ARRB1; ARB1; ARR1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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Application Data (BLM antibody, N-term detects BLM protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BLM protein stained by BLM antibody, N-term () diluted at 1:500.Blue: Hoechst 33342 staining.)

Application Data (BLM antibody detects BLM protein by western blot analysis.A. 30 mug Jurkat whole cell lysate/extract B. 30 mug Raji whole cell lysate/extract C. 30 mug NCI-H929 whole cell lysate/extract5 % SDS-PAGE diluted at 1:500)

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BLM, Polyclonal Antibody (Cat# AAA302258)

Full Name

BLM Antibody

Gene Names

BLM; BS; RECQ2; RECQL2; RECQL3

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Purity

Antibodies were purified by antigen-affinity chromatography.

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of TATDN1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TATDN1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human TATDN1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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TATDN1, Polyclonal Antibody (Cat# AAA256653)

Full Name

Anti-TATDN1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IP (Immunoprecipitation) (THOC7 was immunoprecipitated using:Lane A:0.5 mg Jurkat HeLa Whole Cell Lysate4 uL anti-THOC7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-THOC7 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :24 kDa)

WB (Western Blot) (Anti-THOC7 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa)

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THOC7, Polyclonal Antibody (Cat# AAA256655)

Full Name

Anti-THOC7 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of ZNF24 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZNF24 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

WB (Western Blot) (Anti-ZNF24 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: 293 Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))

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ZNF24, Polyclonal Antibody (Cat# AAA256580)

Full Name

Anti-ZNF24 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunochemical staining of human TNK2 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human TNK2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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ACK1, Polyclonal Antibody (Cat# AAA256144)

Full Name

Anti-ACK1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DDT antibody at 1: 100 dilution.)

Application Data (Sample (30 ug of whole cell lysate) A: Raji 15% SDS PAGE Primary antibody diluted at 1: 500)

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DDT, Polyclonal Antibody (Cat# AAA306689)

Full Name

DDT antibody

Gene Names

DDT; DDCT

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by antigen-affinity chromatography.

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TMED2 antibody at 1: 500 dilution.)

Application Data (Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE Primary antibody diluted at 1: 1000)

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TMED2, Polyclonal Antibody (Cat# AAA306899)

Full Name

TMED2 antibody

Gene Names

TMED2; p24; P24A; RNP24

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by antigen-affinity chromatography.

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of KLRA2 in mouse spleen tissue with KLRA2 antibody at 2.5 ug/mL.)

WB (Western Blot) (Western blot analysis of KLRA2 in mouse spleen tissue lysate with KLRA2 antibody at (A) 0.5 and (B) 1 ug/mL.)

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KLRA2, Polyclonal Antibody (Cat# AAA308293)

Full Name

KLRA2 Antibody

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver damage using MUC16 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using MUC16 at dilution of 1:100 (40x lens).)

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MUC16, Polyclonal Antibody (Cat# AAA313210)

Full Name

MUC16 Rabbit Polyclonal Antibody

Gene Names

MUC16; CA125

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PSMD13 antibody . Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human esophagus using PSMD13 antibody at dilution of 1:100 .)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using PSMD13 antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PSMD13 Antibody at 1:1000 dilution.)

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PSMD13, Polyclonal Antibody (Cat# AAA312864)

Full Name

PSMD13 Polyclonal Antibody

Gene Names

PSMD13; S11; Rpn9; p40.5; HSPC027

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SMO antibodyat 1:1000 dilution.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (40x lens).)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibodyat dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibodyat dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

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SMO, Polyclonal Antibody (Cat# AAA312906)

Full Name

SMO Polyclonal Antibody

Gene Names

SMO; Gx; SMOH; FZD11

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using METTL13 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using METTL13 antibody.)

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METTL13, Polyclonal Antibody (Cat# AAA311474)

Full Name

METTL13 Polyclonal Antibody

Gene Names

EEF1AKNMT; feat; DFNM1; CGI-01; DFNB26; DFNB26M; METTL13; KIAA0859; 5630401D24Rik

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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Application Data

Application Data

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ARHGAP26, Polyclonal Antibody (Cat# AAA307231)

Full Name

ARHGAP26 Antibody

Gene Names

ARHGAP26; GRAF; GRAF1; OPHN1L; OPHN1L1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purified

Pricing

$455/0.1 mL $890/2x0.1 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,870/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/25.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/25.)

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RHEBL1, Polyclonal Antibody (Cat# AAA308482)

Full Name

RHEBL1 Antibody

Gene Names

RHEBL1; RHEBL1c

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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FCM/FACS (Flow Cytometry) (IL10 Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of IL10 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). IL10 (arrow) was detected using the purified Pab.)

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IL10, Polyclonal Antibody (Cat# AAA286719)

Full Name

IL10 Antibody (Center)

Gene Names

IL10; CSIF; TGIF; GVHDS; IL-10; IL10A

Reactivity

Human (Predicted Reactivity: EBV, Monkey)

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Siglec9 C-term Pab is used in Western blot to detect Siglec9 in mouse liver tissue lysate.)

WB (Western Blot) (SIGLEC9 Antibody western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SIGLEC9 antibody detected the SIGLEC9 protein (arrow).)

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SIGLEC9, Polyclonal Antibody (Cat# AAA288044)

Full Name

SIGLEC9 Antibody (C-term)

Gene Names

SIGLEC9; CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of CYP4A11 (4A22) Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (Western blot analysis of anti-CYP4A11 (4A22) Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane).CYP4A11(arrow) was detected using the purified Pab.)

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CYP4A11 (4A22), Polyclonal Antibody (Cat# AAA288068)

Full Name

CYP4A11 (4A22) Antibody (C-term)

Gene Names

CYP4A11; CP4Y; CYP4A2; CYP4AII

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (ITGA5 Antibody (Center) western blot analysis in human uterine tumor and mouse bladder tissue lysates (35ug/lane).This demonstrates the ITGA5 antibody detected the ITGA5 protein (arrow).)

IHC (Immunohiostchemistry) (ITGA5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ITGA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of ITGA5 (arrow) using rabbit polyclonal ITGA5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ITGA5 gene.)

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ITGA5, Polyclonal Antibody (Cat# AAA287509)

Full Name

ITGA5 Antibody (Center)

Gene Names

ITGA5; FNRA; CD49e; VLA-5; VLA5A

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COT Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-COT Pab is used in Western blot to detect COT in HeLa cell lysate.)

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COT (MAP3K8/MEKK8), Polyclonal Antibody (Cat# AAA288655)

Full Name

COT (MAP3K8/MEKK8) Antibody (C-term)

Gene Names

MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (CDKN2C Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDKN2C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of CDKN2C (arrow) using rabbit polyclonal CDKN2C Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2C gene.)

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CDKN2C, Polyclonal Antibody (Cat# AAA288353)

Full Name

CDKN2C Antibody (C-term)

Gene Names

CDKN2C; p18; INK4C; p18-INK4C

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (GPAA1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (GPAA1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPAA1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (GPAA1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the GPAA1 antibody detected the GPAA1 protein (arrow).)

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GPAA1, Polyclonal Antibody (Cat# AAA288376)

Full Name

GPAA1 Antibody (N-term)

Gene Names

GPAA1; GAA1; hGAA1

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of FIBG Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (FIBG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FIBG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of FIBG Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). FIBG (arrow) was detected using the purified Pab.)

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FIBG, Polyclonal Antibody (Cat# AAA288193)

Full Name

FIBG Antibody (C-term)

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.