Active Proteins

AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.

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Application Data (Binding and in vitro activity of murine 18V4F hybridoma antibody.Directly coated chemokines were used here for direct comparisons, however in other experiments CCL3 showed a significantly enhanced signal when biotinylated and coated on streptavidin plates.)

Application Data (Ccr1 ligands are induced after Candida infection and are chemotactic for kidney neutrophils ex vivo.)

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MIP-1 alpha (CCL-3), Active Protein (Cat# AAA76392)

Full Name

Mouse MIP-1 alpha (CCL-3) Recombinant

Gene Names

Ccl3; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha

Purity

>95%, as determined by SDS-PAGE and HPLC

Pricing

$245/0.002 mg $345/0.01 mg $3,060/1 mg $5,970/2x1 mg $8,725/3x1 mg $11,325/4x1 mg $13,435/5x1 mg

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Melanin and Catecholamine Synthesis Pathways

Tyrosinase Activity (Formation of dopachrome-MBTH adduct monitoring at 484nm in a 96-well half area microwell plate with a range of enzyme loads. Maximum signal plateau was reached rapidly at 37 degree C, preventing an accurate time-dependent measurement.)

SDS-PAGE (Lane 1: MW Marker, Lane 2: 2 ug Tyrosinase.)

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Tyrosinase, Active Protein (Cat# AAA77051)

Full Name

Tyrosinase (human), (recombinant) (His-tag)

Gene Names

TYR; CMM8; OCA1A; OCAIA; SHEP3

Purity

>=85% (SDS-PAGE)
Purified by multi-step chromatography

Pricing

$730/0.1 mg $1,425/2x0.1 mg $2,080/3x0.1 mg $2,700/4x0.1 mg $3,095/5x0.1 mg

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Application Data

Application Data

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RANTES (CCL 5), Active Protein (Cat# AAA79168)

Full Name

Rat RANTES (CCL 5)

Gene Names

Ccl5; Scya5; Rantes

Reactivity

Rat

Purity

> 98% by SDS-PAGE & silver stain

Pricing

$355/0.005 mg $525/0.02 mg $1,015/2x0.02 mg $1,485/3x0.02 mg $1,930/4x0.02 mg $2,020/5x0.02 mg

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Bioactivity

SDS-PAGE ((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

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Membrane Cofactor (CD46), Active Protein (Cat# AAA243727)

Full Name

Recombinant Human Membrane Cofactor Protein (CD46)

Gene Names

CD46; MCP; TLX; AHUS2; MIC10; TRA2.10

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

$340/0.02 mg $510/0.1 mg $3,425/1 mg $6,670/2x1 mg $9,745/3x1 mg $12,650/4x1 mg $15,455/5x1 mg

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Bioactivity

SDS-PAGE ((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

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Angiotensin-converting enzyme (ACE2), Active Protein (Cat# AAA244034)

Full Name

Recombinant Macaca fascicularis Angiotensin-converting enzyme (ACE2), partial

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

$470/0.02 mg $1,125/0.1 mg $4,235/1 mg $8,255/2x1 mg $12,060/3x1 mg $15,655/4x1 mg $19,150/5x1 mg

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Application Data (Measured by its binding ability in a functional ELISA. Immobilized human APOH-his at 2 ug/mL (100 ul/well) can bind biotinylated mouse LDLR-his, The EC50 of biotinylated mouse LDLR-his is 26 ng/mL.)

Application Data (Measured by its binding ability in a functional ELISA. Immobilized human APOH-his at 2 ug/mL (100 ul/well) can bind biotinylated human LDLR-his, The EC50 of biotinylated human LDLR-his is 0.35 ug/mL.)

SDS-PAGE

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Apolipoprotein H, Active Protein (Cat# AAA257970)

Full Name

Apolipoprotein H Protein, Human, Recombinant (His Tag)

Gene Names

APOH; BG; B2G1; B2GP1

Purity

>96% as determined by SDS-PAGE

Pricing

$375/0.1 mg $725/2x0.1 mg $1,060/3x0.1 mg $1,370/4x0.1 mg $1,485/5x0.1 mg

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Application Data (Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 ug/ml (100 ul/well) can bind human ALK-4 with a linear range of 0.032-4 ug/ml.)

SDS-PAGE

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ALK4/ACVR1B, Active Protein (Cat# AAA257899)

Full Name

ALK4/ACVR1B Protein, Human, Recombinant (His Tag)

Gene Names

ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4

Purity

>92% as determined by SDS-PAGE

Pricing

$435/0.1 mg $2,310/1 mg $4,500/2x1 mg $6,570/3x1 mg $8,530/4x1 mg $10,195/5x1 mg

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Application Data (Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 50-200 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.)

SDS-PAGE

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ALK-1, Active Protein (Cat# AAA257826)

Full Name

ALK-1 Protein, Human, Recombinant (His Tag)

Gene Names

ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1

Purity

>92% as determined by SDS-PAGE

Pricing

$375/0.1 mg $1,945/1 mg $3,785/2x1 mg $5,535/3x1 mg $7,185/4x1 mg $8,540/5x1 mg

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Application Data (Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.3-2 ug/mL in the presence of 10 ng/mL recombinant Activin A.)

SDS-PAGE

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ACVR2B, Active Protein (Cat# AAA257850)

Full Name

ACVR2B Protein, Human, Recombinant (His Tag)

Gene Names

ACVR2B; HTX4; ACTRIIB; ActR-IIB

Purity

>97% as determined by SDS-PAGE

Pricing

$310/0.1 mg $1,080/0.5 mg $2,095/2x0.5 mg $3,055/3x0.5 mg $3,970/4x0.5 mg $4,650/5x0.5 mg

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Application Data (Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.4-1.6 ug/mL.)

SDS-PAGE

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FGF8, Active Protein (Cat# AAA258053)

Full Name

FGF8 Protein, Human, Recombinant

Gene Names

FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8

Purity

>95% as determined by SDS-PAGE

Pricing

$435/0.05 mg $850/2x0.05 mg $1,235/3x0.05 mg $1,605/4x0.05 mg $1,755/5x0.05 mg

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Application Data (Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-1 ug/mL in the presence of 10 ng/mL recombinant Activin A.)

SDS-PAGE

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ACVR2B, Active Protein (Cat# AAA258265)

Full Name

ACVR2B Protein, Cynomolgus, Recombinant (His Tag)

Purity

>95% as determined by SDS-PAGE

Pricing

$610/0.2 mg $1,180/2x0.2 mg $1,725/3x0.2 mg $2,235/4x0.2 mg $2,530/5x0.2 mg

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Application Data (Measured by its binding ability in a functional ELISA. Immobilized Mouse Angiopoietin-2-His at 2 ug/ml (100 ul/well) can bind mouse TEK-Fc, the EC50 of mouse TEK-Fc is 350-900 ng/mL.)

SDS-PAGE

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Angiopoietin-2, Active Protein (Cat# AAA258152)

Full Name

Angiopoietin-2 Protein, Mouse, Recombinant (His Tag)

Gene Names

Angpt2; Ang2; Agpt2; Ang-2

Purity

>95% as determined by SDS-PAGE

Pricing

$375/0.05 mg $1,565/0.5 mg $3,045/2x0.5 mg $4,455/3x0.5 mg $5,780/4x0.5 mg $6,835/5x0.5 mg

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Application Data (Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.6-3 ug/mL in the presence of 10 ng/ml Recombinant Human Activin A.)

SDS-PAGE

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ACVR2A, Active Protein (Cat# AAA258185)

Full Name

ACVR2A Protein, Mouse, Recombinant (His Tag)

Gene Names

Acvr2a; Acvr2; ActrIIa; TactrII

Purity

>97% as determined by SDS-PAGE
> 95% as determined by SEC-HPLC

Pricing

$435/0.1 mg $2,310/1 mg $4,500/2x1 mg $6,570/3x1 mg $8,530/4x1 mg $10,195/5x1 mg

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Bioactivity (Activating transcription factor 3 (ATF3) is a stress-induced transcription factor that plays vital roles in modulating metabolism, immunity and oncogenesis. ATF3 acts as a hub of the cellular adaptive-response network. Multiple extracellular signals, such as endoplasmic reticulum (ER) stress, cytokines, chemokines, and LPS, are connected to ATF3 induction. Proto-oncogene c-Fos can bind to ATF3 to regulate signal transduction, cell proliferation and differentiation. Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse ATF3 and recombinant human FOS. Briefly, ATF3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FOS-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ATF3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse ATF3 and recombinant human FOS was shown in Figure 1, the EC50 for this effect is 0.13 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Activating Transcription Factor 3 (ATF3), Active Protein (Cat# AAA161885)

Full Name

Active Activating Transcription Factor 3 (ATF3)

Gene Names

Atf3; LRG-21

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$285/0.01 mg $550/0.05 mg $835/0.1 mg $1,015/0.2 mg $1,965/0.5 mg $2,920/1 mg $3,845/2 mg $7,110/5 mg $13,860/2x5 mg $20,255/3x5 mg $26,300/4x5 mg

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Bioactivity (The Semaphorin 3A(SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant rat SEMA3A and recombinant human NRP1 was shown in Figure 1, the EC50 for this effect is 1.22 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Semaphorin 3A (SEMA3A), Active Protein (Cat# AAA161937)

Full Name

Active Semaphorin 3A (SEMA3A)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$240/0.01 mg $465/0.05 mg $710/0.1 mg $870/0.2 mg $1,665/0.5 mg $2,460/1 mg $3,240/2 mg $6,025/5 mg $11,745/2x5 mg $17,160/3x5 mg $22,275/4x5 mg $27,285/5x5 mg

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Bioactivity (Apolipoprotein C1 (APOC1), also known as Apo-CI; ApoC-I; apo-CIB; apoC-IB, is the smallest size apolipoprotein of all apolipoprotein C family (Mr = 6.6 kDa) and located at position 19q13.32. APOC1 is primarily expressed in the liver and activated when monocytes differentiate into macrophages. It plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. It has anti-inflammatory activity and also can promote the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Besides, Apolipoprotein C3 (APOC3) has been identified as an interactor of APOC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOC1 and recombinant mouse APOC3. Briefly, APOC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOC3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APOC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human APOC1 and recombinant mouse APOC3 was shown in Figure 1, the EC50 for this effect is 2.19 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Apolipoprotein C1 (APOC1), Active Protein (Cat# AAA161719)

Full Name

Active Apolipoprotein C1 (APOC1)

Applications

Activity Assay, Cell Culture

Purity

Greater than 80% by SDS-PAGE

Pricing

$255/0.01 mg $490/0.05 mg $740/0.1 mg $905/0.2 mg $1,725/0.5 mg $2,565/1 mg $3,370/2 mg $6,255/5 mg $12,185/2x5 mg $17,810/3x5 mg $23,120/4x5 mg $28,340/5x5 mg

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Bioactivity (Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)

Bioactivity (G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. The activity of G-CSF is usually measured by a cell proliferation assay using M-NFS60 mouse myelogenous leukemia lymphoblast cells. M-NFS60 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant pig G-CSF. After incubated for 4 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 degree C. Proliferation of M-NFS-60 cells after incubation with G-CSF for 4 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant pig G-CSF for 4 days. The result was shown in Figure 2. It was obvious that G-CSF significantly increased cell viability of M-NFS-60 cells. The EC50 is 1 ug/ml)

SDS-PAGE (Figure. SDS-PAGE)

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Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161664)

Full Name

Active Colony Stimulating Factor 3, Granulocyte (GCSF)

Gene Names

CSF3; G-CSF

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$295/0.01 mg $595/0.05 mg $900/0.1 mg $1,100/0.2 mg $2,125/0.5 mg $3,155/1 mg $4,155/2 mg $7,685/5 mg $14,975/2x5 mg $21,885/3x5 mg $28,415/4x5 mg

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Bioactivity (Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. As we know, H2O2 and (NH4)2MoO4 can produce a stable y)

SDS-PAGE (Figure. SDS-PAGE)

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Catalase (CAT), Active Protein (Cat# AAA153115)

Full Name

Active Catalase (CAT)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$210/0.01 mg $365/0.05 mg $535/0.1 mg $650/0.2 mg $1,215/0.5 mg $1,775/1 mg $2,355/2 mg $4,315/5 mg $8,410/2x5 mg $12,295/3x5 mg $15,960/4x5 mg $19,540/5x5 mg

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Bioactivity (Fibroblast growth factor 17(FGF17)  is a member of the fibroblast growth factorfamily. FGF family members possess broad mitogenicand cell survival activities and are involved in a varietyof biological processes, including embryonicdevelopment cell growth,)

SDS-PAGE (Figure. SDS-PAGE)

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Fibroblast Growth Factor 17 (FGF17), Active Protein (Cat# AAA153119)

Full Name

Active Fibroblast Growth Factor 17 (FGF17)

Gene Names

FGF17; HH20; FGF-13; FGF-17

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$210/0.01 mg $370/0.05 mg $550/0.1 mg $665/0.2 mg $1,245/0.5 mg $1,840/1 mg $2,425/2 mg $4,460/5 mg $8,690/2x5 mg $12,695/3x5 mg $16,480/4x5 mg $20,155/5x5 mg

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Bioactivity (Fibroblast growth factor 15 is a protein in mouse encoded by the Fgf15 gene. It is a member of the fibroblast growth factor (FGF) family but, like FGF19, FGF21 and FGF23, has endocrine functions. FGF15 subsequently acts on a cell surface receptor complex)

SDS-PAGE (Figure. SDS-PAGE)

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Fibroblast Growth Factor 15 (FGF15), Active Protein (Cat# AAA153137)

Full Name

Active Fibroblast Growth Factor 15 (FGF15)

Gene Names

Fgf19; Fgf15

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$300/0.01 mg $620/0.05 mg $940/0.1 mg $1,165/0.2 mg $2,250/0.5 mg $3,310/1 mg $4,400/2 mg $8,090/5 mg $15,765/2x5 mg $23,045/3x5 mg $29,915/4x5 mg

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Application Data (Transforming growth factor beta (TGF-) is a multifunctional cytokine belonging to the transforming growth factor superfamily. The TGF- superfamily includes endogenous growth inhibiting proteins; an increase in expression of TGF- often correlates with the malignancy of many cancers and a defect in the cellular growth inhibition response to TGF-. Its immunosuppressive functions then come to dominate, contributing to oncogenesis. To test the effect of TGF- on inhibit HGF-dependent proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1ng/mL HGF prior to the addition of various concentrations of recombinant human TGF-. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. The inhibitory effect of TGF- on HGF-dependent proliferation of HepG2 cells observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant TGF- for 96h. The result was shown in Figure 3. It was obvious that TGF- significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1ug/mL TGF- for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.Figure.The inhibitory effect of TGF- on cell proliferation of HepG2 cells.)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Transforming Growth Factor Beta 2, Active Protein (Cat# AAA150084)

Full Name

Active Transforming Growth Factor Beta 2 (TGFb2)

Gene Names

TGFB2; LDS4; G-TSF; TGF-beta2

Applications

Activity Assay, Cell Culture

Purity

>90%

Pricing

$315/0.01 mg $660/0.05 mg $995/0.1 mg $1,235/0.2 mg $2,395/0.5 mg $3,525/1 mg $4,685/2 mg $8,605/5 mg $16,770/2x5 mg $24,515/3x5 mg $31,820/4x5 mg

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Bioactivity (HTRF assay for Recombinant MRCKb/CDC42BPB (1-473) protein activity 1 uM STK S3 substrate was incubated with different concentrations of MRCKb/CDC42BPB (1-473) protein in a 10 ul reaction system containing 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-STK antibody (1:2) GST and SA-XL665 mixture (each 1:100 diluted with 1X Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Bioactivity (HTRF assay for Recombinant MRCKb/CDC42BPB (1-473) protein activity 1 uM STK S3 substrate was incubated with different concentrations of MRCKb/CDC42BPB (1-473) protein in a 10 ul reaction system containing 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-STK antibody (1:2) GST and SA-XL665 mixture (each 1:100 diluted with 1X Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant MRCKb/CDC42BPB(1-473), protein gel 10% SDS-PAGE Coomassie staining MW: 55.9 kDa Purity: >95%)

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MRCKb/CDC42BPB, Active Protein (Cat# AAA60315)

Full Name

Recombinant MRCKb/CDC42BPB (1-473) protein

Purity

>95%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

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Bioactivity (HTRF assay for RPS6KB1 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant RPS6KB1 protein 10% SDS-PAGE Coomassie staining MW: 60.4 kDa Purity: >= 95%)

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RPS6KB1, Active Protein (Cat# AAA60322)

Full Name

Recombinant RPS6KB1 protein

Purity

>=95%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

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Bioactivity (ADP-Glo assay for SMARCA (636-1131) activity (Data from ICEB Bioscience Inc.) 100 uM ATP and 10 nM DNA was incubated with different concentrations of SMARCA2 (636-1131) protein in a 10 ul reaction system containing 20 mM HEPES pH 7.5,10 mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, 10 ul ADP-Glo Reagent was added to the products and incubated for 1 hour.Then 20 ul Kinase Detection Reagent incubated for 1 hour. All the operations and reactions were performed at RT. Luminescence measurement is collected by BMG.)

SDS-PAGE (Recombinant SMARCA2 (636-1131) protein 10% SDS-PAGE Coomassie staining MW: 82.5 kDa Purity: > 90%)

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SMARCA2, Active Protein (Cat# AAA60346)

Full Name

Recombinant SMARCA2 (636-1131) protein

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Bioactivity (HTRF for MRCKa/CDC42BPA (1-473) activity 1 uM STK S3 substrate was incubated with different concentrations of MRCKa/CDC42BPA (1-473) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant MRCKa/CDC42BPA (1-473) protein 10% SDS-PAGE Coomassie staining MW: 55.7 kDa Purity: >= 95%)

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MRCKa/CDC42BPA, Active Protein (Cat# AAA60314)

Full Name

Recombinant MRCKa/CDC42BPA (1-473) protein

Purity

>=95%

Pricing

$700/0.02 mg $1,365/2x0.02 mg $1,990/3x0.02 mg $2,585/4x0.02 mg $2,920/5x0.02 mg

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Bioactivity

SDS-PAGE (Sample Purity Data.)

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ACE2, Active Protein (Cat# AAA73160)

Full Name

ACE2 (18-615) Protein

Gene Names

ACE2; ACEH

Pricing

$535/0.01 mg $615/0.02 mg $750/0.05 mg $955/0.1 mg $1,860/2x0.1 mg $2,715/3x0.1 mg $3,375/5x0.1 mg $3,525/4x0.1 mg

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Bioactivity (Human SARS-CoV-2 Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay)

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA48384)

Full Name

Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein

Applications

Bioactivity, SDS-PAGE

Purity

>95% by SDS-PAGE

Pricing

$255/0.01 mg $335/0.02 mg $390/0.05 mg $555/0.1 mg $870/0.25 mg $870/5x0.01 mg $1,195/5x0.02 mg $1,380/0.5 mg $1,445/5x0.05 mg $2,185/5x0.1 mg $2,685/2x0.5 mg $3,600/5x0.25 mg $3,920/3x0.5 mg $5,085/4x0.5 mg $5,900/5x0.5 mg

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Bioactivity (Human IL-17E/IL-25 is coated at 2 ug/ml (100 ul/well) can bind Human IL-17 RB (CAT# ATGP4039) in a Functional ELISA assay.)

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stainLane 1 : reducing conditionLane 2 : non-reducing condition)

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IL-17E/IL-25, Active Protein (Cat# AAA48395)

Full Name

Recombinant human IL-17E/IL-25 protein

Gene Names

IL25; IL17E

Applications

Bioactivity, SDS-PAGE

Purity

>95% by SDS-PAGE

Pricing

$290/0.01 mg $380/0.02 mg $450/0.05 mg $660/0.1 mg $990/5x0.01 mg $1,060/0.25 mg $1,410/5x0.02 mg $1,700/0.5 mg $1,730/5x0.05 mg $2,680/5x0.1 mg $3,310/2x0.5 mg $4,450/5x0.25 mg $4,835/3x0.5 mg $6,280/4x0.5 mg $7,345/5x0.5 mg

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Bioactivity (Mouse OX40 Ligand/TNFSF4 (CAT# ATGP4022) is coated at 1 ug/ml (100 ul/well) can bind Human TNFRSF4. The ED50 range )

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

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OX40/TNFRSF4, Active Protein (Cat# AAA48407)

Full Name

Recombinant human OX40/TNFRSF4 protein

Gene Names

TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L

Applications

Bioactivity, SDS-PAGE

Purity

>90% by SDS-PAGE

Pricing

$290/0.01 mg $380/0.02 mg $450/0.05 mg $660/0.1 mg $990/5x0.01 mg $1,060/0.25 mg $1,410/5x0.02 mg $1,700/0.5 mg $1,730/5x0.05 mg $2,680/5x0.1 mg $3,310/2x0.5 mg $4,450/5x0.25 mg $4,835/3x0.5 mg $6,280/4x0.5 mg $7,345/5x0.5 mg

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

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Osteopontin/OPN protein, Active Protein (Cat# AAA48974)

Full Name

Recombinant human Osteopontin/OPN protein

Applications

Bioactivity, SDS-PAGE

Purity

> 90% by SDS-PAGE

Pricing

$215/0.01 mg $250/0.02 mg $290/0.05 mg $360/0.1 mg $520/0.25 mg $690/5x0.01 mg $800/0.5 mg $845/5x0.02 mg $990/5x0.05 mg $1,325/5x0.1 mg $1,550/2x0.5 mg $2,040/5x0.25 mg $2,255/3x0.5 mg $2,930/4x0.5 mg $3,270/5x0.5 mg

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Bioactivity (Mouse M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range is )

SDS-PAGE (3ug by SDS-PAGE under reducing condition andvisualized by coomassie blue stain)

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M-CSF, Active Protein (Cat# AAA48318)

Full Name

Recombinant M-CSF

Gene Names

Csf1; op; Csfm; MCSF; C87615

Applications

Bioactivity, SDS-PAGE

Purity

> 90% by SDS - PAGE

Pricing

$290/0.01 mg $380/0.02 mg $520/0.05 mg $775/0.1 mg $990/5x0.01 mg $1,255/0.25 mg $1,410/5x0.02 mg $2,040/5x0.05 mg $2,060/0.5 mg $3,185/5x0.1 mg $4,010/2x0.5 mg $5,355/5x0.25 mg $5,860/3x0.5 mg $7,605/4x0.5 mg $8,980/5x0.5 mg

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Bioactivity (Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range )

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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TRANCE/RANK L/TNFSF11, Active Protein (Cat# AAA48324)

Full Name

Recombinant TRANCE/RANK L/TNFSF11

Gene Names

Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance

Applications

Bioactivity, SDS-PAGE

Purity

> 90% by SDS - PAGE

Pricing

$310/0.01 mg $400/0.02 mg $580/0.05 mg $875/0.1 mg $1,085/5x0.01 mg $1,410/0.25 mg $1,500/5x0.02 mg $2,305/5x0.05 mg $2,330/0.5 mg $3,620/5x0.1 mg $4,540/2x0.5 mg $6,050/5x0.25 mg $6,630/3x0.5 mg $8,605/4x0.5 mg $10,180/5x0.5 mg

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Bioactivity (SARS-CoV-2 Spike S1 Subunit is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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COVID 19 Spike S1 Subunit Coronavirus, Active Protein (Cat# AAA48370)

Full Name

Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 Subunit protein

Applications

Bioactivity, SDS-PAGE

Purity

>85% by SDS-PAGE

Pricing

$255/0.01 mg $335/0.02 mg $390/0.05 mg $555/0.1 mg $870/0.25 mg $870/5x0.01 mg $1,195/5x0.02 mg $1,445/5x0.05 mg $1,690/2x0.25 mg $2,185/5x0.1 mg $2,465/3x0.25 mg $3,200/4x0.25 mg $3,600/5x0.25 mg

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Bioactivity (SARS-CoV Spike is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

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COVID 19 Spike Coronavirus, Active Protein (Cat# AAA48375)

Full Name

Recombinant SARS-CoV Spike protein

Applications

Bioactivity, SDS-PAGE

Purity

>85% by SDS-PAGE

Pricing

$255/0.01 mg $335/0.02 mg $390/0.05 mg $555/0.1 mg $870/5x0.01 mg $870/0.25 mg $1,195/5x0.02 mg $1,445/5x0.05 mg $1,690/2x0.25 mg $2,185/5x0.1 mg $2,465/3x0.25 mg $3,200/4x0.25 mg $3,600/5x0.25 mg

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Bioactivity (SARS-CoV-2 Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Compare (5 max)

COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA48377)

Full Name

Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein

Applications

Bioactivity, SDS-PAGE

Purity

>95% by SDS-PAGE

Pricing

$255/0.01 mg $335/0.02 mg $390/0.05 mg $555/0.1 mg $870/0.25 mg $870/5x0.01 mg $1,195/5x0.02 mg $1,445/5x0.05 mg $1,690/2x0.25 mg $2,185/5x0.1 mg $2,465/3x0.25 mg $3,200/4x0.25 mg $3,600/5x0.25 mg

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Bioactivity (Canine IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range )

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

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IL-21, Active Protein (Cat# AAA48379)

Full Name

Recombinant canine IL-21 protein

Gene Names

IL21; IL-21

Applications

Bioactivity, SDS-PAGE

Purity

>95% by SDS-PAGE

Pricing

$300/0.01 mg $385/0.02 mg $555/0.05 mg $825/0.1 mg $1,015/5x0.01 mg $1,340/0.25 mg $1,440/5x0.02 mg $2,170/5x0.05 mg $2,195/0.5 mg $3,410/5x0.1 mg $4,275/2x0.5 mg $5,705/5x0.25 mg $6,245/3x0.5 mg $8,105/4x0.5 mg $9,580/5x0.5 mg

SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

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PAI-1, Active Protein (Cat# AAA48474)

Full Name

PAI-1, 24-402aa, Human, His tag, E Coli (Bioactivity Validated)

Gene Names

SERPINE1; PAI; PAI1; PAI-1; PLANH1

Applications

Enzyme Activity, SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

$235/0.02 mg $375/0.1 mg $780/5x0.02 mg $825/0.5 mg $1,380/5x0.1 mg $1,600/2x0.5 mg $2,335/3x0.5 mg $3,025/4x0.5 mg $3,410/5x0.5 mg

SDS-PAGE

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Interleukin-4, Active Protein (Cat# AAA48531)

Full Name

Interleukin- 4, 25-153aa, Human, His tag, E Coli (Bioactivity Validated)

Gene Names

IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

$310/0.01 mg $600/0.05 mg $1,085/5x0.01 mg $1,505/0.25 mg $2,380/5x0.05 mg $2,935/2x0.25 mg $4,290/3x0.25 mg $5,565/4x0.25 mg $6,480/5x0.25 mg

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SDS-PAGE (Figure 1. Human BAFF-R Protein, mFc Tag on SDS-PAGE under reducing condition.)

ELISA (Figure 1. Human BAFF-R Protein, mFc Tag on SDS-PAGE under reducing condition.)

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BAFF-R, Active Protein (Cat# AAA47223)

Full Name

Recombinant Human BAFF-R protein with C-terminal mouse Fc

Gene Names

TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin

Applications

Western Blot

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Pricing

$300/0.01 mg $590/0.05 mg $800/0.1 mg $1,550/2x0.1 mg $2,255/3x0.1 mg $2,930/4x0.1 mg $3,245/5x0.1 mg

What Are Active Proteins?

Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).

If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.

Key Applications of Active Proteins

1. Scientific Research

• Aid in the study of how proteins function in the body


• Aid in understanding various disease processes

2. Drug Development

• Powerful tools to investigate how potential drugs interact with specific proteins


• Ideal for identifying drug targets

3. Cell Culture

• Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)


• Can be used to promote cellular development into specific types (differentiation)

4. Diagnostics

• Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)


• Note: All products are strictly for research-use only (RUO).

5. Therapeutics

• Some active proteins are used directly as treatments (e.g., insulin, enzymes)


• Note: All products are strictly for research-use only (RUO).

6. Vaccine Development

• Used to create or test vaccines by mimicking parts of viruses or bacteria

7. Biochemical Assays

• They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests

Why Buy Active Proteins from AAA Biotech?

• High biological activity – Verified to perform as expected or indicated on datasheet


• Strict quality control – We are confident in our active proteins’ reliability and consistency


• High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components


• Freeze-dried for stability – Long shelf life and straightforward storage


• Mostly tag-free – Closer to natural/native protein form

FAQ

1. What are active proteins used for in research?

Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.

2. How are AAA Biotech's active proteins validated?

AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.

3. Are these proteins tested for biological activity?

Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.