At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
WB (Western Blot) (Western Blot analysis of Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me3/K27ac - biotiny (Lane 2) were detected with antibody anti-H3K4me3, anti-H3K27ac and anti-H4, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K4me3/K27ac - biotin can be detected by anti-H3K4me3 and anti- H3K27ac antibody.)
Application Data (Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin, DNA gel. Recombinant Mononucleosomes H3K4me3/K27a) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact mononucleosomes H3K4me3/K27ac. Intact mononucleosomes H3K4me3/K27ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Mass Spec (Mass Spec analysis for Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin. Synthetic H3K4me3/K27ac protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15357.8 Da. Determined mass = 15357.4 Da.)
Application Data (HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
Application Data (HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
WB (Western Blot) (Recombinant Mononucleosomes H3K4me2 (EPL) tested by Western blot. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me2 (EPL) (Lane 2) were detected with anti-H3K4me2 antibody and anti-H4 antibody , respectively. H3 was detected as loading control. Only Recombinant Mononucleosomes H3K4me2 (EPL) can be detected by anti-H3K4me2 antibody.)
Application Data (Recombinant Mononucleosomes H3K4me2 (EPL) - DNA gel Mononucleosomes were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact nucleosome. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
Application Data (HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80% )
Application Data (HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
WB (Western Blot) (Western Blot analysis for Recombinant Mono-nucleosomes H3K36me3 (MLA) Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K36me3 (MLA) (Lane 2) were detected with H3K36me3 antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K36me3 (MLA) can be detected by H3K36me3 antibody.)
Application Data (Recombinant Mononucleosomes H3K36me3 (MLA) agarose gel Recombinant Mononucleosomes H3K36me3 (MLA) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K36me3. Intact mononucleosomes H3K36me3 migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Application Data (HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant IKKe protein 10% SDS-PAGE Coomassie staining MW: 81.7 kDa Purity: >90% )
Application Data (HTRF assay for PRDM9 (191-415) activity 1 uM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product. )
Application Data (HTRF assay for PRDM9 (191-415) activity 1 uM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product. )
Application Data (HTRF assay for BRD3 (24-144), GST-Tag activity 3 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD3 (24-144), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRD3 (24-144), GST-Tag activity 3 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD3 (24-144), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD3 (24-244), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.6 kDa Purity: >85%)
Application Data (HTRF assay for BRD4 (333-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (333-460), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRD4 (333-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (333-460), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD4 (333-460), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 41.1 kDa Purity: >91%)
Application Data (HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRDT (21-137) protein, GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.2 kDa Purity: >80%)
Application Data (Mass Spec analysis for Recombinant Mononucleosomes H3S10ph (EPL) Histone H3S10ph (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15306 Da. Determined mass = 15306 Da.)
Application Data (Recombinant Mononucleosomes H3S10ph (EPL) DNA gel Mononucleosomes H3S10ph (EPL) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3S10ph. Lane 3: 601 DNA. Intact mononucleosome H3S10ph migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3S10ph (EPL) SDS PAGE gel 13% SDS-PAGE gel, stained with Coomassie blue. Purity: ? 92%)
WB (Western Blot) (Recombinant KAT6A / MOZ (488-778) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6A / MOZ (488-778) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3 pan-acetyl antibody .)
SDS-PAGE (Recombinant KAT6A / MOZ (488-778) protein gel 10% SDS-PAGE gel with Coomassie staining MW: 35.5 kDa Purity: > 95%)
SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes (H1.2) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Then beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.5 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Coomassie Blue. * indicates streptavidin. The SDS-PAGE gel result showed that about 60% of biotinylated mononucleosomes were pulled down by streptavidin beads.)
SDS-PAGE (Recombinant Mononucleosomes (H1.2) - biotin 13% SDS-PAGE gel with Coomassie Blue staining Purity: ?95%)
Application Data (Recombinant Mononucleosomes (H1.2) - biotin DNA gel Recombinant Mononucleosomes (H1.2) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes (H1.2) - biontinylated. Intact mononucleosomes (H1.2) - biotinylated migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
WB (Western Blot) (Western blot for Recombinant KAT5 protein activity 2 ?g Recombinant Mononucleosomes H3.1 were incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody and H3 antibody . Western blot was used to detect the production of acetylated histone H4. H3 was detected as a loading control.)
WB (Western Blot) (Western blot for Recombinant KAT5 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated histone H4.)
Application Data (HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
Application Data (HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
SDS-PAGE (Recombinant JAK2 (532-1132) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 71 kDa Purity: >90%)
Application Data (HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant SETD1B-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1B-SET: 35.7 kDa MW of WDR5: 36.6 kDa MW of RBBP5: 59.2 kDa MW of ASH2L: 68.7 kDa Purity: >90%)
Application Data (HTRF assay for LYN activity 1 uM TK substrate was incubated with different concentrations of LYN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant LYN protein 10% SDS-PAGE gel with Coomassie blue staining MW: 57 kDa Purity: >90%)
Application Data (HTRF assay for FYN (2-537) activity 1 uM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
Application Data (HTRF assay for FYN (2-537) activity 1 uM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
SDS-PAGE (Recombinant FYN (2-537) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 61.6 kDa Purity: >95%)
Application Data (HTRF assay for FLT3 (571-993) activity 1 uM TK substrate was incubated with different concentrations of FLT3 (571-993) protein in a 10u?l reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1mM MnCl, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for FLT3 (571-993) activity 1 uM TK substrate was incubated with different concentrations of FLT3 (571-993) protein in a 10u?l reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1mM MnCl, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant FLT3 (571-993) protein 10% SDS-PAGE with Coomassie staining MW: 49.7 kDa Purity: >50%)
Application Data (HTRF assay for GRK5 activity 1 uM STK S3 substrate was incubated with different concentrations of GRK5 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 2 mg/ml Casein, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
SDS-PAGE (Recombinant GRK5 protein 10% SDS-PAGE with Coomassie staining MW: 68.8 kDa Purity: >50%)
Application Data (AMP-Glo assay for UBE2G1 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2G1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data (HTRF assay for NTRK1 (440-796, G667C) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, G667C) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for NTRK1 (440-796, G667C) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, G667C) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (NTRK1 (440-796, G667C) protein gel. 10% SDS-PAGE gel stained with Coomassie Blue. MW: 41.32kDa Purity: >90%)
Application Data (AMP-Glo assay for UBE2K activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2K in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2K protein gel. 12.5% SDS-PAGE Coomassie blue MW: 24.57kDa Purity: >95%)
Application Data (AMP-Glo assay for UBE2D4 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2D4 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2D4 protein gel. UBE2D4 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 18.81kDa Purity: >95%)
Application Data (AMP-Glo assay for UBE2B activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2B in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2B protein gel. UBE2B protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.48kDa Purity: >95%)
Application Data (AMP-Glo assay for UBE2S activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2S in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
ELISA (ELISA for PARP3 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP3 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
SDS-PAGE (Recombinant PARP3 protein gel 10% SDS-PAGE Comassie staining MW: 64.6kDa Purity: >95%)
Application Data (AMP-Glo assay for UBE2L6 activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2L6 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2L6 protein gel. Recombinant UBE2L6 was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.93kDa Purity: >95%)
Application Data (HTRF assay for EPHB4 (561-987) activity 1uM TK substrate was incubated with different concentrations of EPHB4 (564-987) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
ELISA (ELISA for ADPRS/ARH3 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRS/ARH3 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA (ELISA for ADPRS/ARH3 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRS/ARH3 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
Application Data (GTPase-Glo assay for KRAS (1-186) activity 1uM rGTP and 0.5 mM DTT was incubated with different concentrations of KRAS (1-186) in GTPase/ GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5ul. To the completed GTPase reactions, we added 5ul of reconstituted GTPase-Glo Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10ul) was added, reactions were incubated for 10 minutes and luminescence was measured.)
SDS-PAGE (Recombinant KRAS (1-186) protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue MW: 22.36kDa Purity: >90%)
Application Data (SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 ug/mL RBD, aa319-541 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 ug/mL RBD, aa319-541. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.)
SDS-PAGE (Recombinant SARS-CoV-2 Spike protein, S1 (aa319-541) protein gel. 1 ug of SARS-CoV-2 Spike protein was run on a 4-12% SDS-PAGE gel, stained with Coomassie blue. MW: 40 kDa Purity: >90%)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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