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Recombinant Proteins

At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.


Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.


Viewing 5250-5300 of 14112 product results


product-image-AAA153569_AD11.jpg Amino Acid Info

Carbonic Anhydrase IX (CA9), Recombinant Protein (Cat# AAA153569)

Full Name
Recombinant Carbonic Anhydrase IX (CA9)
Applications
Western Blot
Purity
Greater than 80% by SDS-PAGE
Pricing
product-image-AAA153576_AD13.jpg Amino Acid Info

Ribonuclease III, Nuclear (RNASEN), Recombinant Protein (Cat# AAA153576)

Full Name
Recombinant Ribonuclease III, Nuclear (RNASEN)
Gene Names
DROSHA; RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA153582_AD13.jpg Amino Acid Info

Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), Recombinant Protein (Cat# AAA153582)

Full Name
Recombinant Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1)
Gene Names
COX4I1; COX4; COXIV; COX4-1
Applications
Western Blot
Purity
Greater than 80% by SDS-PAGE
Pricing
product-image-AAA153587_AD13.jpg Amino Acid Info

Protein Disulfide Isomerase A4 (PDIA4), Recombinant Protein (Cat# AAA153587)

Full Name
Recombinant Protein Disulfide Isomerase A4 (PDIA4)
Gene Names
Pdia4; Cai; Erp72; ERp-72; AI987846
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA161959_SDS_PAGE13.jpg SDS-PAGE (Figure. SDS-PAGE)

Complement Component 3a (C3a), Recombinant Protein (Cat# AAA161959)

Full Name
Recombinant Complement Component 3a (C3a)
Applications
Western Blot
Purity
Greater than 80% by SDS-PAGE
Pricing
product-image-AAA161975_SDS_PAGE13.jpg SDS-PAGE (Figure. SDS-PAGE)

Actin Gamma 2, Smooth Muscle (ACTg2), Recombinant Protein (Cat# AAA161975)

Full Name
Recombinant Actin Gamma 2, Smooth Muscle (ACTg2)
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA161994_SDS_PAGE13.jpg SDS-PAGE (Figure. SDS-PAGE)

Cortistatin (CORT), Recombinant Protein (Cat# AAA161994)

Full Name
Recombinant Cortistatin (CORT)
Gene Names
CORT; CST-14; CST-17; CST-29
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
product-image-AAA55764_SDS_PAGE15.jpg SDS-PAGE

Squamous Cell Carcinoma Antigen 1, Recombinant Protein (Cat# AAA55764)

Full Name
Recombinant Human Squamous Cell Carcinoma Antigen 1 (SCCA1)
Purity
> 90 % as determined by SDS-PAGE.
Pricing
product-image-AAA60100_WB11.jpg WB (Western Blot) (Western Blot analysis of Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me3/K27ac - biotiny (Lane 2) were detected with antibody anti-H3K4me3, anti-H3K27ac and anti-H4, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K4me3/K27ac - biotin can be detected by anti-H3K4me3 and anti- H3K27ac antibody.)

Mononucleosomes H3K4me3/H3K27ac, Recombinant Protein (Cat# AAA60100)

Full Name
Recombinant Mononucleosomes H3K4me3/H3K27ac-biotin
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
product-image-AAA60104_AD11.jpg Application Data (HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)

SUV39H2, Recombinant Protein (Cat# AAA60104)

Full Name
Recombinant SUV39H2 protein
Gene Names
SUV39H2; KMT1B
Pricing
product-image-AAA60121_WB11.jpg WB (Western Blot) (Recombinant Mononucleosomes H3K4me2 (EPL) tested by Western blot. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me2 (EPL) (Lane 2) were detected with anti-H3K4me2 antibody and anti-H4 antibody , respectively. H3 was detected as loading control. Only Recombinant Mononucleosomes H3K4me2 (EPL) can be detected by anti-H3K4me2 antibody.)

Mononucleosomes H3K4me2, Recombinant Protein (Cat# AAA60121)

Full Name
Recombinant Mononucleosomes H3K4me2 (EPL)
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
product-image-AAA60137_AD11.jpg Application Data (HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

YTHDF3, Recombinant Protein (Cat# AAA60137)

Full Name
Recombinant YTHDF3 (407-560) protein
Pricing
product-image-AAA60140_AD11.jpg Application Data (HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

TBP, Recombinant Protein (Cat# AAA60140)

Full Name
Recombinant TBP protein
Gene Names
TBP; HDL4; GTF2D; SCA17; TFIID; GTF2D1
Pricing
product-image-AAA60142_WB11.jpg WB (Western Blot) (Western Blot analysis for Recombinant Mono-nucleosomes H3K36me3 (MLA) Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K36me3 (MLA) (Lane 2) were detected with H3K36me3 antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K36me3 (MLA) can be detected by H3K36me3 antibody.)

Mononucleosomes H3K36me3, Recombinant Protein (Cat# AAA60142)

Full Name
Recombinant Mononucleosomes H3K36me3 (MLA)
Gene Names
HIST1H3A; H3/A; H3FA
Pricing
product-image-AAA60143_AD11.jpg Application Data (HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)

IKKe, Recombinant Protein (Cat# AAA60143)

Full Name
Recombinant IKKe protein
Gene Names
IKBKE; IKKE; IKKI; IKK-E; IKK-i; KIAA0151; MGC125294; MGC125295; MGC125297
Pricing
product-image-AAA60144_AD11.jpg Application Data (HTRF assay for PRDM9 (191-415) activity 1 uM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product. )

PRDM9, Recombinant Protein (Cat# AAA60144)

Full Name
Recombinant PRDM9 (191-415) protein
Gene Names
PRDM9; PFM6; MSBP3; ZNF899; MEISETZ
Pricing
product-image-AAA60160_AD11.jpg Application Data (HTRF assay for BRD3 (24-144), GST-Tag activity 3 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD3 (24-144), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

BRD3, Recombinant Protein (Cat# AAA60160)

Full Name
Recombinant BRD3 (24-144) protein, GST-Tag
Gene Names
BRD3; ORFX; RING3L
Pricing
product-image-AAA60163_AD11.jpg Application Data (HTRF assay for BRD4 (333-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (333-460), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

BRD4, Recombinant Protein (Cat# AAA60163)

Full Name
Recombinant BRD4 (333-460) protein, GST-Tag
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Pricing
product-image-AAA60165_AD11.jpg Application Data (HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

BRDT, Recombinant Protein (Cat# AAA60165)

Full Name
Recombinant BRDT (21-137) protein, GST-Tag
Gene Names
BRDT; CT9; BRD6; SPGF21
Pricing
product-image-AAA60168_AD11.jpg Application Data (Mass Spec analysis for Recombinant Mononucleosomes H3S10ph (EPL) Histone H3S10ph (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15306 Da. Determined mass = 15306 Da.)

Mononucleosomes H3S10ph, Recombinant Protein (Cat# AAA60168)

Full Name
Recombinant Mononucleosomes H3S10ph (EPL)
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
product-image-AAA60180_WB13.jpg WB (Western Blot) (Recombinant KAT6A / MOZ (488-778) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6A / MOZ (488-778) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3 pan-acetyl antibody .)

KAT6A/MOZ, Recombinant Protein (Cat# AAA60180)

Full Name
Recombinant KAT6A/MOZ (488-778) protein
Gene Names
KAT6A; MOZ; MYST3; ZNF220; RUNXBP2; ZC2HC6A
Pricing
product-image-AAA60200_SDS_PAGE11.jpg SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes (H1.2) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Then beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.5 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Coomassie Blue. * indicates streptavidin. The SDS-PAGE gel result showed that about 60% of biotinylated mononucleosomes were pulled down by streptavidin beads.)

Mononucleosomes, Recombinant Protein (Cat# AAA60200)

Full Name
Recombinant Mononucleosomes (H1.2)-biotin
Gene Names
HIST1H1C; H1C; H1.2; H1F2; H1s-1
Pricing
product-image-AAA60202_WB11.jpg WB (Western Blot) (Western blot for Recombinant KAT5 protein activity 2 ?g Recombinant Mononucleosomes H3.1 were incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody and H3 antibody . Western blot was used to detect the production of acetylated histone H4. H3 was detected as a loading control.)

KAT5, Recombinant Protein (Cat# AAA60202)

Full Name
Recombinant KAT5 protein
Gene Names
KAT5; TIP; ESA1; PLIP; TIP60; cPLA2; HTATIP; ZC2HC5; HTATIP1
Pricing
product-image-AAA60212_AD11.jpg Application Data (HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)

JAK2, Recombinant Protein (Cat# AAA60212)

Full Name
Recombinant JAK2 (532-1132) protein
Gene Names
JAK2; JTK10; THCYT3
Pricing
product-image-AAA60221_SDS_PAGE15.jpg SDS-PAGE (Recombinant NUAK1 / ARK5 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 76 kDa Purity: >95%)

NUAK1, Recombinant Protein (Cat# AAA60221)

Full Name
Recombinant NUAK1 (ARK5) protein
Gene Names
NUAK1; ARK5
Pricing
product-image-AAA60223_AD11.jpg Application Data (HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SETD1B-SET, Recombinant Protein (Cat# AAA60223)

Full Name
Recombinant SETD1B-SET complex
Gene Names
ASH2L; ASH2; Bre2; ASH2L1; ASH2L2
Pricing
product-image-AAA60238_AD13.jpg Application Data (HTRF assay for LYN activity 1 uM TK substrate was incubated with different concentrations of LYN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

LYN, Recombinant Protein (Cat# AAA60238)

Full Name
Recombinant LYN protein
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Pricing
product-image-AAA60239_AD11.jpg Application Data (HTRF assay for FYN (2-537) activity 1 uM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)

FYN, Recombinant Protein (Cat# AAA60239)

Full Name
Recombinant FYN (2-537) protein
Pricing
product-image-AAA60251_AD11.jpg Application Data (HTRF assay for FLT3 (571-993) activity 1 uM TK substrate was incubated with different concentrations of FLT3 (571-993) protein in a 10u?l reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1mM MnCl, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

FLT3, Recombinant Protein (Cat# AAA60251)

Full Name
Recombinant FLT3 (571-993) protein
Gene Names
FLT3; FLK2; STK1; CD135; FLK-2
Pricing
product-image-AAA60252_AD13.jpg Application Data (HTRF assay for GRK5 activity 1 uM STK S3 substrate was incubated with different concentrations of GRK5 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 2 mg/ml Casein, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)

GRK5, Recombinant Protein (Cat# AAA60252)

Full Name
Recombinant GRK5 protein
Gene Names
GRK5; GPRK5
Pricing
product-image-AAA60380_AD13.jpg Application Data (AMP-Glo assay for UBE2G1 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2G1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)

UBE2G1, Recombinant Protein (Cat# AAA60380)

Full Name
Recombinant UBE2G1 protein
Purity
>=95% by SDS-PAGE.
Pricing
product-image-AAA60385_AD11.jpg Application Data (HTRF assay for NTRK1 (440-796, G667C) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, G667C) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

NTRK1, Recombinant Protein (Cat# AAA60385)

Full Name
Recombinant NTRK1 (440-796, G667C) protein
Pricing
product-image-AAA60391_AD13.jpg Application Data (AMP-Glo assay for UBE2K activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2K in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)

UBE2K, Recombinant Protein (Cat# AAA60391)

Full Name
Recombinant UBE2K protein
Purity
>=95% by SDS-PAGE.
Pricing
product-image-AAA60397_AD13.jpg Application Data (AMP-Glo assay for UBE2D4 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2D4 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)

UBE2D4, Recombinant Protein (Cat# AAA60397)

Full Name
Recombinant UBE2D4 protein
Purity
>=95% by SDS-PAGE.
Pricing
product-image-AAA60400_AD13.jpg Application Data (AMP-Glo assay for UBE2B activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2B in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)

UBE2B, Recombinant Protein (Cat# AAA60400)

Full Name
Recombinant UBE2B protein
Purity
>=95% by SDS-PAGE.
Pricing
product-image-AAA60407_AD13.jpg Application Data (AMP-Glo assay for UBE2S activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2S in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)

UBE2S, Recombinant Protein (Cat# AAA60407)

Full Name
Recombinant UBE2S protein
Purity
>=90% by SDS-PAGE.
Pricing
product-image-AAA60410_ELISA13.jpg ELISA (ELISA for PARP3 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP3 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)

PARP-3, Recombinant Protein (Cat# AAA60410)

Full Name
Recombinant PARP-3 protein
Pricing
product-image-AAA60361_AD13.jpg Application Data (AMP-Glo assay for UBE2L6 activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2L6 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)

UBE2L6, Recombinant Protein (Cat# AAA60361)

Full Name
Recombinant UBE2L6 protein
Purity
>=95% by SDS-PAGE.
Pricing
product-image-AAA60367_AD13.jpg Application Data (HTRF assay for EPHB4 (561-987) activity 1uM TK substrate was incubated with different concentrations of EPHB4 (564-987) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

EPHB4, Recombinant Protein (Cat# AAA60367)

Full Name
Recombinant EPHB4 (561-987) protein
Pricing
product-image-AAA60368_ELISA11.jpg ELISA (ELISA for ADPRS/ARH3 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRS/ARH3 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)

ADPRS/ARH3, Recombinant Protein (Cat# AAA60368)

Full Name
Recombinant ADPRS/ARH3 protein
Pricing
product-image-AAA60374_AD13.jpg Application Data (GTPase-Glo assay for KRAS (1-186) activity 1uM rGTP and 0.5 mM DTT was incubated with different concentrations of KRAS (1-186) in GTPase/ GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5ul. To the completed GTPase reactions, we added 5ul of reconstituted GTPase-Glo Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10ul) was added, reactions were incubated for 10 minutes and luminescence was measured.)

KRAS, Recombinant Protein (Cat# AAA60374)

Full Name
Recombinant KRAS (1-186) protein
Pricing
product-image-AAA60224_AD13.jpg Application Data (SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 ug/mL RBD, aa319-541 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 ug/mL RBD, aa319-541. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.)

COVID 19 Spike, S1 RBD Coronavirus, Recombinant Protein (Cat# AAA60224)

Full Name
Recombinant SARS-CoV-2 Spike protein, S1 (RBD aa319-541)
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Pricing

HIV-1 p24, Recombinant, Recombinant Protein (Cat# AAA58336)

Full Name
HIV-1 p24, Recombinant
Purity
Purity verified by SDS-PAGE. Purity compares with reference lot.
Pricing

Borrelia burgdorferi flagellin, Recombinant Protein (Cat# AAA58346)

Full Name
B. burgdorferi Recombinant
Applications
ELISA
Purity
> 95% pure (SDS-PAGE)
Pricing

Hepatitis B Surface, Recombinant Protein (Cat# AAA58404)

Full Name
HBsAg (adr) Recombinant
Purity
> = 95% Pure (RP-HPLC. Size exclusion and ion-exhcange chromatography.
Pricing
product-image-AAA72897_SDS_PAGE15.gif SDS-PAGE (The purity was determined to be >75% by densitometry. Approx. MW 140kDa)

KAT3A (CREBBP), Recombinant Protein (Cat# AAA72897)

Full Name
KAT3A (CREBBP) recombinant protein
Gene Names
CREBBP; CBP; RSTS; KAT3A
Applications
Western Blot
Purity
>75%
Pricing
product-image-AAA72923_SDS_PAGE15.gif SDS-PAGE (The purity of MGMT was determined to be >95% by densitometry)

MGMT, Recombinant Protein (Cat# AAA72923)

Full Name
MGMT recombinant protein
Applications
Western Blot
Purity
>95%
Pricing
product-image-AAA72934_SDS_PAGE15.jpg SDS-PAGE (The purity was determined to be >80% by densitometry. Approx. MW 58kDa)

CDK2, Recombinant Protein (Cat# AAA72934)

Full Name
CDK2 recombinant protein
Gene Names
CDK2; p33(CDK2)
Purity
>80%
Pricing
product-image-AAA72954_AD13.gif Application Data

KIAA1549-BRAF, Recombinant Protein (Cat# AAA72954)

Full Name
KIAA1549-BRAF Mutant (Kex16Bex9), Active
Applications
Kinase Assay
Pricing
product-image-AAA72956_AD13.gif Application Data

SRGAP3-BRAF, Recombinant Protein (Cat# AAA72956)

Full Name
SRGAP3-BRAF (Sex12Bex9), Active
Gene Names
SRGAP3; WRP; MEGAP; SRGAP2; ARHGAP14
Applications
Kinase Assay
Pricing

What Are Recombinant Proteins?


Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.


The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.


Common Applications of Recombinant Proteins


  • Studying protein-protein and protein-DNA interactions

  • Functional assays to study biological pathways

  • Standard curves in ELISA and other quantitative assays

  • Use as antigens for antibody production

  • Development and screening of therapeutic drugs

  • Biomarker discovery and validation

  • Cell signaling and immune response studies

  • Vaccine research and development


Key Features of AAA Biotech’s Recombinant Proteins


  • High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.

  • Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.

  • Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.

  • Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.

  • Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.

  • Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.

  • Flexible Quantities: Available in various pack sizes to suit different experimental needs.

  • Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.


Why Buy Recombinant Proteins from AAA Biotech?


At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.


With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.


FAQ


1. How are AAA Biotech recombinant proteins validated?


Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.


2. Are your proteins suitable for therapeutic development or only research?


AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.


3. What types of expression systems do you use for recombinant protein production?


The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.


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