At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
ELISA (Biotinylated Human B7-2/CD86 Protein ELISA DataImmobilized Biotinylated Human B7-2, His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.2?g/ml determined by ELISA.)
Tris-Bis-PAGE (Biotinylated Human B7-2/CD86 Protein Tris-Bis PAGEBiotinylated Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Biotinylated Human PD-1/PDCD1 Protein SPR DataHuman PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 ?M as determined in SPR assay (Biacore T200).)
ELISA (Biotinylated Human PD-1/PDCD1 Protein ELISA DataImmobilized Biotinylated Human PD-1, His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.)
Application Data (Biotinylated Human PD-1/PDCD1 Protein SEC-HPLCThe purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human PD-1/PDCD1 Protein Tris-Bis PAGEBiotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.)
ELISA (Biotinylated Human IL-18BP Protein ELISA DataImmobilized Human IL-18 at 2?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-18BP, His Tag with the EC50 of 6.1ng/ml determined by ELISA.)
Application Data (Biotinylated Human IL-18BP Protein SEC-HPLCThe purity of Biotinylated Human IL-18BP is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human IL-18BP Protein Tris-Bis PAGEBiotinylated Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human LILRB3/CD85a/ILT5 Protein ELISA DataImmobilized Biotinylated Human LILRB3, His Tag at 2?g/ml (100?l/well) on the streptavidin precoated plate (5?g/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.)
Application Data (Biotinylated Human LILRB3/CD85a/ILT5 Protein SEC-HPLCThe purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human LILRB3/CD85a/ILT5 Protein Tris-Bis PAGEBiotinylated Human LILRB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human IL-2 R alpha/CD25 Protein ELISA DataImmobilized Anti-CD25 Antibody, hFc Tag at 0.2?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA.)
Application Data (Biotinylated Human IL-2 R alpha/CD25 Protein SEC-HPLCThe purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human IL-2 R alpha/CD25 Protein Tris-Bis PAGEBiotinylated Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Biotinylated Human EGFR/HER1 Protein SPR DataBiotinylated Human EGFR, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).)
Application Data (Biotinylated Human EGFR/HER1 Protein SEC-HPLCThe purity of Biotinylated Human EGFR/HER1 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human EGFR/HER1 Protein Tris-Bis PAGEBiotinylated Human EGFR/HER1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Figure-2: Comparative binding of Spike S1 variants to sACE2: Wells of a 96-well microtiter plate were coated with 100 ng in duplicates each of S1-WT, S1-N439K, S1-D614G, S1-South Africa, and S1-Southern California. Binding to sACE2 was determined by adding different concentrations of biotinylated-sACE2.)
Application Data (Figure-1: Recombinant SARS-CoV-2 Spike S1 wild type (WT), N439K, D614G, K417N/E484K/N501Y and S13l/W152C/L452R mutant proteins were run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Application Data (Figure-2: Wells of a 96-microtiter plate were coated with 4ug/ml of SARS-Cov-2/nCov/COVID-19 Nucleocapsid recombinant protein. The binding was detected by addition of different dilution of polyclonal antibody. The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.)
SDS-PAGE (Figure-1: SDS PAGE- SARS-CoV-2 Nucleocapsid recombinant protein. 2 ug protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Structure (Structural diagrams of spike glycoproteins of SARS-CoV, MERS-CoV, and SARS-CoV-2. All spike proteins of coronaviruses contain S1 subunit and S2 subunit, which were divided by the S cleavage sites. FP, fusion peptide; HR, heptad repeat 1 and heptad repeat 2; RBD, receptor-binding domain, contains core binding motif in the external subdomain; SP, signal peptide. Adapted from Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2. Liu Z, et al. J Med Virol. 2020 Feb 26. doi: 10.1002/jmv.25726.)
Application Data (Figure 2: Binding assay of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein with recombinant Human ACE2 Fc Protein. An ELISA plate was coated with 1ug/ml of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein, and the plate was incubated overnight at 4 degree C. Different concentrations (100 ng/well to 0 ng/well) of recombinant Human ACE2 Fc Protein was added to the wells. HRP conjugated goat anti-human Fc was used as secondary antibody.)
SDS-PAGE (Figure-1: SDS-PAGE analysis of purified SARS-CoV-2 Spike S1-His recombinant protein. 0.5, 1.0 and 2.0ug protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Application Data (SDS-PAGE analysis of recombinant mouse soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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