At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant LCK protein 10% SDS-PAGE with Coomassie staining MW: 59.3 kDa Purity: >90%)
Application Data (MTase-Glo assay for PRMT9 activity1uM histone H4 was incubated with different concentrations of PRMT9 protein in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 1?M SAM at room temperature for 1 hour. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation. SAH standard curve (0-1uM) was performed following the same protocol.)
Application Data (MTase-Glo assay for PRMT9 activity1uM histone H4 was incubated with different concentrations of PRMT9 protein in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 1?M SAM at room temperature for 1 hour. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation. SAH standard curve (0-1uM) was performed following the same protocol.)
Application Data (HTRF assay for Recombinant MBD1-MBD protein activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD1-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant MRM1-MBD protein 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 10.11kDa Purity: >95%)
ELISA (ELISA for ADPRHL1/ARH2 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRHL1/ARH2 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA (ELISA for ADPRHL1/ARH2 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRHL1/ARH2 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
Application Data (AMP-Glo assay for UBE2R1 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2R1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2R1 protein gel. UBE2D3 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 28.9kDa Purity: >95%)
Application Data (MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
Application Data (MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
SDS-PAGE (Recombinant METTL1/WDR4 protein gel. METTL1/WDR4 protein was run on a 12.5% SDS-PAGE gel and stained with Coomasse Blue. MW: WDR4: 48.8kDa METTL1: 31.47kDa Purity: >90%)
Application Data (ADP-Glo assay for MAP2K7 (MEK7) activity 1 uM JNK2 was incubated with different concentrations of MAP2K7 (MEK7) protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at 25 degree C for 60min. Then 20 ul Kinase Detection Reagent incubates at 25 degree C for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE (Recombinant MAP2K7 (MEK7) protein gel. Recombinant MAP2K7 protein was run on an 10 % SDS-PAGE gel and stained with Coomassie blue. MW: 48.77kDa Purity: > 95%)
Application Data (HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant PRKCB protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 78.3kDa Purity: >95%)
ELISA (ELISA for ZC3HAV1/PARP13 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP13 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
SDS-PAGE (Human GPC3 Protein, hFc Tag on SDS-PAGE under reducing condition.)
ELISA (ELISA plate pre-coated by 2 ug/mL (100 uL/well) Human GPC3 Protein, hFc Tag (AAA47529) can bind Anti-GPC3 Neutralizing antibody in a linear range of 0.92–58.59 ng/mL.)
ELISA (ELISA plate pre-coated by 1 ug/mL (100 uL/well) Human GPC3 Protein, hFc Tag (AAA47529) can bind Anti-GPC3 Neutralizing antibody in a linear range of 1.12-508.85 ng/mL.)
SDS-PAGE (Human NEFL(9-88) Protein, hFc Tag on SDS-PAGE under reducing condition.)
ELISA (ELISA plate pre-coated by 1 ug/mL (100 uL/well) Human NEFL (9-88) Protein, hFc Tag (AAA47533) can bind Anti-NEFL(9-88) antibody(DM198), Rabbit mAb in a linear range of 0.128-80 ng/mL.)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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