At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
WB (Western Blot) (Recombinant KAT2A (GCN5) protein activity assay0.5 ug Histone H3.1 (Cat. No. please inquire) was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) GCN5 catalytic domain in 30 ul reaction system for 2 hours at room temperature. Products were detected by Western blot. H3ac (pan-acetyl) antibody (Cat. No. please inquire) was used to recognize acetylated histone H3.)
SDS-PAGE (Recombinant KAT2A (GCN5) protein, SDS-PAGE gel Recombinant KAT2A (GCN5) protein was run on a 10% SDS-PAGE gel and stained with Coomassie BlueMW: 95.6 kDa Purity: > 85%)
Application Data (HTRF assay for BRD4 (44-460) activity 3 uM H4K5/8/12/16(4ac) peptide was incubated with different concentrations of BRD4 (44-460) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF was used to detect products.)
Application Data (HTRF assay for BRD4 (44-460) activity 3 uM H4K5/8/12/16(4ac) peptide was incubated with different concentrations of BRD4 (44-460) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF was used to detect products.)
SDS-PAGE (Recombinant BRD4 (44-460) protein gel 10% SDS-PAGE gel, stained with Coomassie blue. MW: 51.6 kDa Purity: > 75% )
WB (Western Blot) (Western Blot analysis of Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me3/K27ac - biotiny (Lane 2) were detected with antibody anti-H3K4me3, anti-H3K27ac and anti-H4, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K4me3/K27ac - biotin can be detected by anti-H3K4me3 and anti- H3K27ac antibody.)
Application Data (Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin, DNA gel. Recombinant Mononucleosomes H3K4me3/K27a) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact mononucleosomes H3K4me3/K27ac. Intact mononucleosomes H3K4me3/K27ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Mass Spec (Mass Spec analysis for Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin. Synthetic H3K4me3/K27ac protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15357.8 Da. Determined mass = 15357.4 Da.)
Application Data (HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
Application Data (HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
WB (Western Blot) (Recombinant Mononucleosomes H3K4me2 (EPL) tested by Western blot. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me2 (EPL) (Lane 2) were detected with anti-H3K4me2 antibody and anti-H4 antibody , respectively. H3 was detected as loading control. Only Recombinant Mononucleosomes H3K4me2 (EPL) can be detected by anti-H3K4me2 antibody.)
Application Data (Recombinant Mononucleosomes H3K4me2 (EPL) - DNA gel Mononucleosomes were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact nucleosome. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
Application Data (HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80% )
Application Data (HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
WB (Western Blot) (Western Blot analysis for Recombinant Mono-nucleosomes H3K36me3 (MLA) Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K36me3 (MLA) (Lane 2) were detected with H3K36me3 antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K36me3 (MLA) can be detected by H3K36me3 antibody.)
Application Data (Recombinant Mononucleosomes H3K36me3 (MLA) agarose gel Recombinant Mononucleosomes H3K36me3 (MLA) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K36me3. Intact mononucleosomes H3K36me3 migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Application Data (HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant IKKe protein 10% SDS-PAGE Coomassie staining MW: 81.7 kDa Purity: >90% )
Application Data (HTRF assay for PRDM9 (191-415) activity 1 uM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product. )
Application Data (HTRF assay for PRDM9 (191-415) activity 1 uM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product. )
Application Data (HTRF assay for BRD3 (24-144), GST-Tag activity 3 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD3 (24-144), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRD3 (24-144), GST-Tag activity 3 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD3 (24-144), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD3 (24-244), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.6 kDa Purity: >85%)
Application Data (HTRF assay for BRD4 (333-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (333-460), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRD4 (333-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (333-460), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD4 (333-460), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 41.1 kDa Purity: >91%)
Application Data (HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRDT (21-137) protein, GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.2 kDa Purity: >80%)
Application Data (Mass Spec analysis for Recombinant Mononucleosomes H3S10ph (EPL) Histone H3S10ph (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15306 Da. Determined mass = 15306 Da.)
Application Data (Recombinant Mononucleosomes H3S10ph (EPL) DNA gel Mononucleosomes H3S10ph (EPL) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3S10ph. Lane 3: 601 DNA. Intact mononucleosome H3S10ph migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3S10ph (EPL) SDS PAGE gel 13% SDS-PAGE gel, stained with Coomassie blue. Purity: ? 92%)
Application Data (Recombinant TRIM33 (959-1069) protein HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of TRIM33 (959- 1069) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (Recombinant TRIM33 (959-1069) protein HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of TRIM33 (959- 1069) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant TRIM33 (959-1069) protein gel. TRIM33 (959-1069) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.7 kDa Purity: >80%)
Application Data (HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
Application Data (HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
SDS-PAGE (Recombinant TRIM24 (862-980) protein gel.TRIM24 (862-980) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 18.8 kDa Purity: > 95% )
Application Data (Recombinant BRD3 (306-416) activity assay. 3.3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRD3 (306-416) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SDS-PAGE (Recombinant BRD3 (306-416) protein gel. BRD3 (306-416) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
Application Data (HTRF assay for BRD3 (24-144) activity 3 uM H4K5/8/12/16(4ac) peptide was incubated with different concentrations of BRD3 (24-144) protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each at 1:100 dilution in Binding Buffer) were added to the reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRD3 (24-144) activity 3 uM H4K5/8/12/16(4ac) peptide was incubated with different concentrations of BRD3 (24-144) protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each at 1:100 dilution in Binding Buffer) were added to the reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD3 (24-144) protein gel. BRD3 (24-144) protein was run on a 12.5%SDS-PAGE gel and stained with Coomassie Blue. MW: 20.5 kDa Purity: > 85%)
Application Data (Recombinant BRD9 (130-259) activity assay. 3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with BRD7 (130-259) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SDS-PAGE (Recombinant BRD9 (130-259) protein gel. BRD9 (130-259) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.)
WB (Western Blot) (HMT Assay for Recombinant SETD2 (1392-2564) protein activity. 2 ug recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody). SETD2 (1392-2564) alone was used as negative control.)
SDS-PAGE (Recombinant SETD2 (1392-2564) protein gel. SETD2 (1392-2564) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 134 kDa. Purity: > 70%)
Application Data (Recombinant DNMT3A / DNMT3L complex activity assay Recombinant DNMT3A / DNMT3L complex activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
SDS-PAGE (Recombinant DNMT3A / DNMT3L complex gel Recombinant DNMT3A / DNMT3L complex was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (Recombinant DNMT3B / DNMT3L complex activity assay Assay was performed using DNMT Activity / Inhibition Assay. Add 100 ul reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubation was for 2 hour at 37 degree C.)
SDS-PAGE (Recombinant DNMT3B / DNMT3L complex gel Recombinant DNMT3B / DNMT3L complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue. MW: DNMT3B: 89.7 kDa DNMT3L: 48.6 kDa Purity: > 91%)
Application Data (Recombinant TET2 (1129-2002) activity assay 500 nM of 30 bp dsDNA oligos (sequence: 5’-CAGTAACTGTGGTC/5mC/GGTAACTGACTTGCA-3’) were incubated with different concentrations of TET2 (1129-2002) protein in a 20 ?l reaction system containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 100 ?M ascorbate, 100 ?M alpha-ketoglutarate, 100 ?M ATP, 1 mM TCEP at room temperature for 2 hours. 20 ?l reaction product was concentrated to 50 ul, then 1 ?l product was spotted onto positively charged nylon membrane. 5hmC antibody was used to detect the generation of products.)
SDS-PAGE (Recombinant TET2 (1129-2002) protein gel. Recombinant TET2 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 102.7 kDa Purity: >45%)
Application Data (Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 196 kDa Purity: >60%)
Application Data (Recombinant IDH2 (R140Q) protein activity assay 20 uM NADPH and 1 uM a-Ketoglutaric acid were incubated with 500 nM IDH2 (R140Q) protein in a reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA at room temperature. Depletion of NADPH was monitored continuously at absorbance 340 nm for 60 min.)
WB (Western Blot) (Recombinant KAT6A / MOZ (488-778) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6A / MOZ (488-778) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3 pan-acetyl antibody .)
SDS-PAGE (Recombinant KAT6A / MOZ (488-778) protein gel 10% SDS-PAGE gel with Coomassie staining MW: 35.5 kDa Purity: > 95%)
SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes (H1.2) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Then beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.5 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Coomassie Blue. * indicates streptavidin. The SDS-PAGE gel result showed that about 60% of biotinylated mononucleosomes were pulled down by streptavidin beads.)
SDS-PAGE (Recombinant Mononucleosomes (H1.2) - biotin 13% SDS-PAGE gel with Coomassie Blue staining Purity: ?95%)
Application Data (Recombinant Mononucleosomes (H1.2) - biotin DNA gel Recombinant Mononucleosomes (H1.2) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes (H1.2) - biontinylated. Intact mononucleosomes (H1.2) - biotinylated migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
WB (Western Blot) (Western blot for Recombinant KAT5 protein activity 2 ?g Recombinant Mononucleosomes H3.1 were incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody and H3 antibody . Western blot was used to detect the production of acetylated histone H4. H3 was detected as a loading control.)
WB (Western Blot) (Western blot for Recombinant KAT5 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated histone H4.)
Application Data (HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
Application Data (HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
SDS-PAGE (Recombinant JAK2 (532-1132) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 71 kDa Purity: >90%)
Application Data (HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant SETD1B-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1B-SET: 35.7 kDa MW of WDR5: 36.6 kDa MW of RBBP5: 59.2 kDa MW of ASH2L: 68.7 kDa Purity: >90%)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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