At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (WB Suggested Anti-TSFM Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate.TSFM is strongly supported by BioGPS gene expression data to be expressed in HeLa)
WB (Western Blot) (Host: RabbitTarget Name: TSFMSample Type: Human MCF7Antibody Dilution: 1.0ug/mlTSFM is strongly supported by BioGPS gene expression data to be expressed in MCF7)
WB (Western Blot) (Host: RabbitTarget Name: TSFMSample Type: Human 721_BAntibody Dilution: 1.0ug/mlTSFM is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
WB (Western Blot) (Host: RabbitTarget Name: TSFMSample Type: Human 293TAntibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that TSFM is expressed in HEK293T)
IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of GDF11 (arrow) using rabbit polyclonal GDF11 Antibody (R47). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GDF11 gene (Lane 2) (Origene Technologies).)
IHC (Immunohiostchemistry) (GMFG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GMFG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of GMFG Antibody (C-term) in CEM cell line lysates (35ug/lane). GMFG (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (Mouse Gsk3a Antibody (C-term) (AAA289355)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lymph node(mandibular) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Gsk3a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Mouse Gsk3a Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3a antibody detected the Gsk3a protein (arrow).)
IHC (Immunohiostchemistry) (TRIM4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (TRIM4 Antibody (N-term) western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).This demonstrates the TRIM4 antibody detected the TRIM4 protein (arrow).)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of UTY Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry) (UTY Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of UTY Antibody (C-term) in mouse brain tissue lysates (35ug/lane). UTY (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (SPACA5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPACA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (SPACA5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SPACA5 antibody detected the SPACA5 protein (arrow).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NCI-H292 cells using OPRS1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of OPRS1 (arrow) using rabbit polyclonal OPRS1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OPRS1 gene (Lane 2) (Origene Technologies).)
FCM/FACS (Flow Cytometry) (RARA Antibody (C-term)(AAA289217) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry) (RARA Antibody (C-term) (AAA289217) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of RARA Antibody (C-term) (AAA289217) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot) (All lanes : Anti-RARA Antibody (C-term) at1:1000 dilution Lane 1: HL-60 whole cell lysateLane 2: MCF-7 whole cell lysate Lane 3: HepG2whole cell lysate Lane 4: Hela whole cell lysateLane 5: T47D whole cell lysate Lysates/proteinsat 20 ?g per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot) (RARA Antibody (C-term) (AAA289217) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow).)
FCM/FACS (Flow Cytometry) (SMPD2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of SMPD2 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). SMPD2 (arrow) was detected using the purified Pab.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Prostate Apoptosis Response Protein-4 antibody.)
WB (Western Blot) (Western blot analysis of extracts from 293 cells The lane on the left is treated with synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysate from SW620 cell line,using Prostate Apoptosis Response protein-4 Antibody(C0309). C0309 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
WB (Western Blot) (RAB5B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the RAB5B antibody detected the RAB5B protein (arrow).)
WB (Western Blot) (RAB5B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RAB5B antibody detected the RAB5B protein (arrow).)
FCM/FACS (Flow Cytometry) (CYP2E1 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP2E1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of CYP2E1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CYP2E1 (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (NAT14 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT14 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of lysate from K562 cell line, using NAT14 Antibody (N-term). AAA288417 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IF (Immunofluorescence) (Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.)
WB (Western Blot) (TGM2 Antibody (Center K444) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MDA-231 cells using DLGAP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of DLGAP1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). DLGAP1 (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (CDK8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of CDK8 (arrow) using rabbit polyclonal CDK8 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK8 gene.)
IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
WB (Western Blot) (HNRNPD Antibody (N-term) western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow).)
FCM/FACS (Flow Cytometry) (ZYG11A Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of ZYG11A Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). ZYG11A (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of EYA2 (arrow) using rabbit polyclonal EYA2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EYA2 gene.)
WB (Western Blot) (EYA2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EYA2 antibody detected the EYA2 protein (arrow).)
IHC (Immunohiostchemistry) (SCYL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCYL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (SCYL1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCYL1 antibody detected the SCYL1 protein (arrow).)
FCM/FACS (Flow Cytometry) (EFTUD1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry) (EFTUD1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EFTUD1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (EFTUD1 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the EFTUD1 antibody detected the EFTUD1 protein (arrow).)
WB (Western Blot) (EFTUD1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EFTUD1 antibody detected the EFTUD1 protein (arrow).)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of GAD2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry) (GAD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (GAD2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow).)
WB (Western Blot) (Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody (Center). AAA289987 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
FCM/FACS (Flow Cytometry) (CSF3R Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CSF3R Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of CSF3R Antibody (Center) in HL-60 cell line lysates (35ug/lane). CSF3R (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of HMGN3 (arrow) using rabbit polyclonal HMGN3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN3 gene.)
WB (Western Blot) (HMGN3 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the HMGN3 antibody detected the HMGN3 protein (arrow).)
FCM/FACS (Flow Cytometry) (TNFRSF10D Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of TNFRSF10D Antibody (Center) in HL-60 cell line lysates (35ug/lane). TNFRSF10D (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (UROC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UROC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (UROC1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UROC1 antibody detected the UROC1 protein (arrow).)
IHC (Immunohiostchemistry) (WBSCR27 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WBSCR27 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (WBSCR27 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the WBSCR27 antibody detected the WBSCR27 protein (arrow).)
WB (Western Blot) (Western blot analysis of LMX1B (arrow) using rabbit polyclonal LMX1B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LMX1B gene.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with LMX1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of LMX1B Antibody (Center) in mouse heart tissue lysates (35ug/lane). LMX1B (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (PTOV1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PTOV1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (PTOV1 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PTOV1 antibody detected the PTOV1 protein (arrow).)
IHC (Immunohiostchemistry) (ZDHHC20 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZDHHC20 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (ZDHHC20 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ZDHHC20 antibody detected the ZDHHC20 protein (arrow).)
IHC (Immunohiostchemistry) (EAPP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EAPP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (EAPP Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the EAPP antibody detected the EAPP protein (arrow).)
IHC (Immunohiostchemistry) (ROBLD3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROBLD3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (ROBLD3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ROBLD3 antibody detected the ROBLD3 protein (arrow).)
IHC (Immunohiostchemistry) (Mouse Fgfr3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Fgfr3 Antibody (Center) (AAA288856M for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Mouse Fgfr3 Antibody (Center) (AAA288856) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Fgfr3 antibody detected the Fgfr3 protein (arrow).)
FCM/FACS (Flow Cytometry) (GAGE7 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (GAGE7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAGE7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of GAGE7 Antibody (C-term) in K562 cell line lysates (35ug/lane). GAGE7 (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (PPL Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (PPL Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PPL antibody detected the PPL protein (arrow).)
IHC (Immunohistochemistry) (TCF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TCF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry) (TCF3 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (TCF3 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).)
WB (Western Blot) (TCF3 Antibody (C-term) western blot analysis in human uterine tumor tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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