Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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WB (Western Blot) (Western blot analysis FOXA1 using LOVO whole cell lysates)

WB (Western Blot) (Western blot analysis of extracts from HepG2, using FOXA1 Antibody.)

IF (Immunofluorescence) (AAA327405 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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FOXA1, Polyclonal Antibody (Cat# AAA327405)

Full Name

FOXA1 Antibody

Gene Names

FOXA1; HNF3A; TCF3A

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of AAA327409 expression in various lysates)

WB (Western Blot) (Western blot analysis of P2RX7 using Jurkat whole cell lysates)

IHC (Immunohistochemistry) (AAA327409 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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P2RX7, Polyclonal Antibody (Cat# AAA327409)

Full Name

P2RX7 Antibody

Gene Names

P2RX7; P2X7

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

WB (Western Blot) (Western blot analysis of CD7 using Jurkat whole cell lysates)

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CD7, Polyclonal Antibody (Cat# AAA327413)

Full Name

CD7 Antibody

Gene Names

CD7; GP40; TP41; Tp40; LEU-9

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA327417 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of BGN using Jurkat whole cell lysates)

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BGN, Polyclonal Antibody (Cat# AAA327417)

Full Name

BGN Antibody

Gene Names

BGN; PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA327419 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis NUDC using MCF7 whole cell lysates)

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NUDC, Polyclonal Antibody (Cat# AAA327419)

Full Name

NUDC Antibody

Gene Names

NUDC; HNUDC; MNUDC; NPD011

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of HDLBP using K562 whole cell lysates)

IHC (Immunohistochemistry) (AAA327424 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HDLBP, Polyclonal Antibody (Cat# AAA327424)

Full Name

HDLBP Antibody

Gene Names

HDLBP; HBP; VGL; PRO2900

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of DUSP3 using MCF7 whole cell lysates)

IHC (Immunohistochemistry) (AAA327428 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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DUSP3, Polyclonal Antibody (Cat# AAA327428)

Full Name

DUSP3 Antibody

Gene Names

DUSP3; VHR

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of CTSS using HuvEc whole cell lysates)

IHC (Immunohistochemistry) (AAA327431 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CTSS, Polyclonal Antibody (Cat# AAA327431)

Full Name

CTSS Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis OS9 using RAW264.7 whole cell lysates)

IHC (Immunohistochemistry) (AAA327432 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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OS9, Polyclonal Antibody (Cat# AAA327432)

Full Name

OS9 Antibody

Gene Names

OS9; OS-9; ERLEC2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis GRK1 using HuvEc whole cell lysates)

IF (Immunofluorescence) (AAA327433 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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GRK1, Polyclonal Antibody (Cat# AAA327433)

Full Name

GRK1 Antibody

Gene Names

GRK1; RK; RHOK; GPRK1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

WB (Western Blot) (Western blot analysis of PPP1R9A using NIH-3T3 whole cell lysates)

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PPP1R9A, Polyclonal Antibody (Cat# AAA327434)

Full Name

PPP1R9A Antibody

Gene Names

PPP1R9A; NRB1; NRBI; Neurabin-I

Reactivity

Human

Applications

Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA327440 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohiostchemistry) (AAA327440 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

WB (Western Blot) (Western blot analysis F5 using A549 whole cell lysates)

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F5, Polyclonal Antibody (Cat# AAA327440)

Full Name

F5 Antibody

Gene Names

F5; FVL; PCCF; THPH2; RPRGL1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of CD82 using RAW264.7 whole cell lysates)

IHC (Immunohistochemistry) (AAA327442 at 1/200 staining human lymph cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CD82, Polyclonal Antibody (Cat# AAA327442)

Full Name

CD82 Antibody

Gene Names

CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis SLC9A3R1 using COLO205 whole cell lysates)

IHC (Immunohistochemistry) (AAA327445 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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SLC9A3R1, Polyclonal Antibody (Cat# AAA327445)

Full Name

SLC9A3R1 Antibody

Gene Names

SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA327450 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis S100P using Jurkat whole cell lysates)

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S100P, Polyclonal Antibody (Cat# AAA327450)

Full Name

S100P Antibody

Gene Names

S100P; MIG9

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Detection of human and mouse TCEB2 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-TCEB2 antibody AAA212445 (lot AAA212445-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation) (Detection of human TCEB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TCEB2 antibody AAA212445 (lot AAA212445-1) used for IP at 6 ug/mg lysate. TCEB2 was inefficiently immunoprecipitated by rabbit anti-TCEB2 antibody BL14445. For blotting immunoprecipitated TCEB2, AAA212445 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohiostchemistry) (Detection of human TCEB2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCEB2 (Cat. No. AAA212445 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of mouse TCEB2 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-TCEB2 (Cat. No. AAA212445 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

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TCEB2, Polyclonal Antibody (Cat# AAA212445)

Full Name

Rabbit anti-TCEB2 Antibody, Affinity Purified

Gene Names

TCEB2; ELOB; SIII

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $605/0.1 mg $940/5x0.01 mg $1,170/2x0.1 mg $1,710/3x0.1 mg $2,220/4x0.1 mg $2,475/5x0.1 mg

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WB (Western Blot) (Detection of human and mouse BZW2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BZW2 antibody AAA212806 (lot AAA212806-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation) (Detection of human BZW2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BZW2 antibody AAA212806 (lot AAA212806-1) used for IP at 6 ug per reaction. BZW2 was also immunoprecipitated by rabbit anti-BZW2 antibody BL17565. For blotting immunoprecipitated BZW2, AAA212806 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

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BZW2, Polyclonal Antibody (Cat# AAA212806)

Full Name

Rabbit anti-BZW2 Antibody, Affinity Purified

Gene Names

BZW2; MST017; HSPC028; MSTP017

Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $605/0.1 mg $940/5x0.01 mg $1,170/2x0.1 mg $1,710/3x0.1 mg $2,220/4x0.1 mg $2,475/5x0.1 mg

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human prostate using TCEB1 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using TCEB1 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TCEB1 at 1:1000 dilution.)

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TCEB1, Polyclonal Antibody (Cat# AAA313797)

Full Name

TCEB1 Rabbit Polyclonal Antibody

Gene Names

TCEB1; SIII; eloC

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using CACNB2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using CACNB2 at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using CACNB2 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse heart, using CACNB2 at 1:1000 dilution.)

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CACNB2, Polyclonal Antibody (Cat# AAA313634)

Full Name

CACNB2 Rabbit Polyclonal Antibody

Gene Names

CACNB2; MYSB; CAVB2; CACNLB2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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FCM/FACS (Flow Cytometry) (TBC1D4 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TBC1D4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of TBC1D4 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). TBC1D4 (arrow) was detected using the purified Pab.)

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TBC1D4, Polyclonal Antibody (Cat# AAA289380)

Full Name

TBC1D4 Antibody (N-term)

Gene Names

TBC1D4; AS160; NIDDM5

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (PKLR Antibody (S8) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).)

WB (Western Blot) (The anti-PKLR Pab is used in Western blot to detect PKLR in NIH/3T3 cell lysate.)

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PKLR, Polyclonal Antibody (Cat# AAA288937)

Full Name

PKLR Antibody (N-term)

Gene Names

PKLR; PK1; PKL; PKR; RPK; PKRL

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-NF?B-p105/p50 (S337) Polyclonal Antibody diluted at 1:500)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using NF-?B p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the NF-?B p105/p50 (Phospho-Ser337) peptide.)

WB (Western Blot) (Western blot analysis of NF-?B p105/p50 (Phospho-Ser337) Antibody. The lane on the right is blocked with the NF-?B p105/p50 (Phospho-Ser337) peptide.)

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NFkappaB-p105/p50, Polyclonal Antibody (Cat# AAA318642)

Full Name

NFkappaB-p105/p50 (Phospho-Ser337) Rabbit Polyclonal Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-IRS-1 (S307) Polyclonal Antibody diluted at 1:1000)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser307) Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using IRS-1 (Phospho-Ser307) Antibody. The picture on the right is blocked with the IRS-1 (Phospho-Ser307) peptide.)

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IRS-1, Polyclonal Antibody (Cat# AAA318646)

Full Name

IRS-1 (Phospho-Ser307) Rabbit Polyclonal Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

WB (Western Blot) (Western Blot analysis of 1 Hela treated with LPS, 2 Hela,using primary antibody at 1:1000 dilution. Secondary antibody)

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EPHA1, Polyclonal Antibody (Cat# AAA318657)

Full Name

EPHA1 (Phospho-Tyr605) Rabbit Polyclonal Antibody

Gene Names

EPHA1; EPH; EPHT; EPHT1

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

WB (Western Blot) (Western blot analysis of lysate from A549 cells, using SP-B antibody)

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SP-B, Polyclonal Antibody (Cat# AAA318493)

Full Name

SP-B Antibody

Gene Names

SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of FSHR expression in HUVEC cell extracts)

IF (Immunofluorescence) (AAA322108 staining HUVEC by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA322108 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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FSHR, Polyclonal Antibody (Cat# AAA322108)

Full Name

FSHR Antibody

Gene Names

FSHR; LGR1; ODG1; FSHR1; FSHRO

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (TTR/Transthyretin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemisry) (TTR/Transthyretin Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohiostchemistry) (TTR/Transthyretin Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (TTR/Transthyretin Antibody-Anti-TTR antibody (AAA163045, 100 ug/mL) yields a specific band on capillary Western analysis (Protein Simple, WES 12-230 kDa separation module) in 1 ng purified recombinant human TTR protein.)

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TTR/Transthyretin, Polyclonal Antibody (Cat# AAA163045)

Full Name

TTR/Transthyretin Rabbit anti-Human Polyclonal Antibody

Gene Names

TTR; CTS; CTS1; PALB; TBPA; HsT2651

Reactivity

Human

Applications

Simple Western Blot, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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FCM/FACS (Flow Cytometry) (Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.)

IF (Immunofluorescence) (Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

IF (Immunofluorescence) (Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry) (Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)

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CCL2 / MCP1, Polyclonal Antibody (Cat# AAA162177)

Full Name

Anti-CCL2 / MCP1 Antibody (aa62-89) IHC-plus

Gene Names

CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Ammonium sulfate precipitation

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

WB (Western Blot) ((Figure. Western Blot; Sample: Recombinant protein.))

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Myelin Basic Protein (MBP), Polyclonal Antibody (Cat# AAA132115)

Full Name

Biotin-Linked Antibody to Myelin Basic Protein (MBP)

Reactivity

Human, Mouse, Rat, Pig

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography.

Pricing

$215/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,445/5 mL $2,815/2x5 mL $4,115/3x5 mL $5,335/4x5 mL $6,500/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue)

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Brain Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant CTNNb1, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Lung Tissue.)

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Catenin Beta 1 (CTNNb1), Polyclonal Antibody (Cat# AAA132019)

Full Name

Polyclonal Antibody to Catenin Beta 1 (CTNNb1)

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $290/0.2 mL $460/0.5 mL $570/1 mL $1,525/5 mL $2,975/2x5 mL $4,350/3x5 mL $5,645/4x5 mL $6,865/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant APOD, Human.)

WB (Western Blot) (Western Blot: Sample: Human Serum.)

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Apolipoprotein D (APOD), Polyclonal Antibody (Cat# AAA131581)

Full Name

Polyclonal Antibody to Apolipoprotein D (APOD)

Reactivity

Human, Mouse, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using DPYSL3 Polyclonal Antibody at dilution of 1:55(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using DPYSL3 Polyclonal Antibody at dilution of 1:55(×200))

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DPYSL3, Polyclonal Antibody (Cat# AAA175330)

Full Name

DPYSL3 Polyclonal Antibody

Gene Names

DPYSL3; DRP3; ULIP; CRMP4; DRP-3; LCRMP; CRMP-4; ULIP-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using RUVBL1 Polyclonal Antibody at dilution of 1:65(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using RUVBL1 Polyclonal Antibody at dilution of 1:65(×200))

WB (Western Blot) (Western blot analysis of 293T HepG2 K562 HT29 A549 and Raji cell lysates using RUVBL1 Polyclonal Antibody at dilution of 1:2000)

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RUVBL1, Polyclonal Antibody (Cat# AAA176412)

Full Name

RUVBL1 Polyclonal Antibody

Gene Names

RUVBL1; RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using ABHD4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Rat testis using ABHD4 Polyclonal Antibody at 1:1000 dilution.)

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ABHD4, Polyclonal Antibody (Cat# AAA179125)

Full Name

ABHD4 Polyclonal Antibody

Gene Names

ABHD4; ABH4

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human lung cancer using RAB22A Polyclonal Antibody at dilution of 1:100)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer using RAB22A Polyclonal Antibody at dilution of 1:100)

WB (Western Blot) (Western Blot analysis of Mouse heart tissue using RAB22A Polyclonal Antibody at dilution of 1:300)

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RAB22A, Polyclonal Antibody (Cat# AAA168152)

Full Name

RAB22A Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-LPCAT1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)

WB (Western Blot) (Host: RabbitTarget Name: LPCAT1Sample Tissue: Human Ovary TumorAntibody Dilution: 1.0ug/ml)

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LPCAT1, Polyclonal Antibody (Cat# AAA200047)

Full Name

LPCAT1 antibody - middle region

Gene Names

LPCAT1; AYTL2; lpcat; AGPAT9; PFAAP3; AGPAT10; LPCAT-1; lysoPAFAT

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rat, Zebrafish

Applications

Western Blot

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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WB (Western Blot) (Figure 1. Western blot analysis of HDAC9 using anti-HDAC9 antibody (AAA125639).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysatesLane 2: rat liver tissue lysatesLane 3: mouse testis tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse RAW264. 7 whole cell lysatesLane 6: human THP-1 whole cell lysatesLane 7: human HL-60 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HDAC9 antigen affinity purified polyclonal antibody (Catalog # AAA125639) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC9 at approximately 150KD. The expected band size for HDAC9 is at 150KD.)

FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of THP-1 cells using anti-HDAC9 antibody (AAA125639).Overlay histogram showing THP-1 cells stained with AAA125639 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC9 Antibody (AAA125639,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of HDAC9 using anti-HDAC9 antibody (AAA125639).HDAC9 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-HDAC9 Antibody (AAA125639) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

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HDAC9, Polyclonal Antibody (Cat# AAA125639)

Full Name

Anti-HDAC9 Antibody

Gene Names

HDAC9; HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL

Reactivity

Human, Mouse, Rat

Applications

Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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WB (Western Blot) (Figure 1. Western blot analysis of Ccr2 using anti-Ccr2 antibody.)

IHC (Immunohiostchemistry)

IHC (Immunohistochemistry)

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CCR2, Polyclonal Antibody (Cat# AAA125392)

Full Name

Anti-CCR2 Antibody

Gene Names

Ccr2; Ckr2; Ccr2a; Ccr2b; Ckr2a; Ckr2b; mJe-r; Cmkbr2; Cc-ckr-2

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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WB (Western Blot) (Figure 1. Western blot analysis of EPHB2 using anti-EPHB2 antibody.)

IF (Immunofluorescence)

FCM/FACS (Flow Cytometry)

FCM/FACS (Flow Cytometry)

FCM/FACS (Flow Cytometry)

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Eph receptor B2/EPHB2, Polyclonal Antibody (Cat# AAA125428)

Full Name

Anti-Eph receptor B2/EPHB2 Antibody

Gene Names

EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; BDPLT22

Reactivity

Human, Mouse, Rat

Applications

Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of CACO-2 cells using anti-Sorbitol Dehydrogenase/ SORD antibody (AAA125795).Overlay histogram showing CACO-2 cells stained with AAA125795 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD Antibody (AAA125795, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of Sorbitol Dehydrogenase/ SORD using anti- Sorbitol Dehydrogenase/ SORD antibody (AAA125795).Sorbitol Dehydrogenase/ SORD was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Sorbitol Dehydrogenase/ SORD Antibody (AAA125795) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (AAA125795).Sorbitol Dehydrogenase/ SORD was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Sorbitol Dehydrogenase/ SORD Antibody (AAA125795) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (AAA125795).Sorbitol Dehydrogenase/ SORD was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Sorbitol Dehydrogenase/ SORD Antibody (AAA125795) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (AAA125795).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysatesLane 2: human U937 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human A431 whole cell lysatesLane 5: monkey kidney tissue lysatesLane 6: rat liver tissue lysatesLane 7: rat kidney tissue lysatesLane 8: mouse liver tissue lysatesLane 9: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD antigen affinity purified polyclonal antibody (Catalog # AAA125795) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/ SORD at approximately 43KD. The expected band size for Sorbitol Dehydrogenase/ SORD is at 38KD.)

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Sorbitol Dehydrogenase/SORD, Polyclonal Antibody (Cat# AAA125795)

Full Name

Anti-Sorbitol Dehydrogenase/SORD Antibody

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat, Monkey

Applications

Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot of PRKCA Antibody (N-term) in mouse NIH-3T3 cell line lysates (35 ug/lane). PRKCA (arrow) was detected using the purified Pab.)

FCM/FACS (Flow Cytometry) (PRKCA Antibody (N-term) flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescence of PRKCA Antibody (N-term)with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry) (Anti-PRKCA / PKC-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

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PRKCA / PKC-Alpha, Polyclonal Antibody (Cat# AAA52389)

Full Name

Rabbit Polyclonal to Human PRKCA / PKC-Alpha

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse

Applications

Flow Cytometry, Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Ammonium sulfate precipitation

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. XIAP antibody diluted at 1:1000.)

IF (Immunofluorescence) (Immunofluorescence of methanol-fixed HeLa, using BIRC4 antibody at 1:100 dilution.)

IHC (Immunohistochemistry) (Anti-XIAP antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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XIAP, Polyclonal Antibody (Cat# AAA52061)

Full Name

Rabbit Polyclonal to Human XIAP

Gene Names

XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ILK Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using ILK Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ILK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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ILK, Polyclonal Antibody (Cat# AAA36647)

Full Name

ILK Polyclonal Antibody

Gene Names

ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TNFRSF1B antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of THP-1 cells, using TNFRSF1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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TNFRSF1B, Polyclonal Antibody (Cat# AAA36694)

Full Name

TNFRSF1B Polyclonal Antibody

Gene Names

TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver cancer using SDHA antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat heart using SDHA antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SDHA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

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SDHA, Polyclonal Antibody (Cat# AAA37327)

Full Name

SDHA Polyclonal Antibody

Gene Names

SDHA; FP; PGL5; SDH1; SDH2; SDHF; CMD1GG

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

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NCLN, Polyclonal Antibody (Cat# AAA39489)

Full Name

NCLN Antibody

Gene Names

NCLN; NET59

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

NCLN Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of TOCA-1 in Human Brain cells with TOCA-1 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemical staining of human brain tissue using TOCA-1 antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of TOCA-1 in human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 0.5 μg/mL.)

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TOCA-1, Polyclonal Antibody (Cat# AAA39752)

Full Name

TOCA-1 Antibody

Gene Names

FNBP1L; TOCA1; C1orf39

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

TOCA-1 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of AIF in human retina tissue with AIF antibody at 10 μg/mL.)

WB (Western Blot) (Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 μg/mL.)

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AIF, Polyclonal Antibody (Cat# AAA39783)

Full Name

AIF Antibody

Gene Names

CNKSR1; CNK; CNK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

AIF Antibody is Ion exchange chromatography purified.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of BAP31 in rat lung tissue with BAP31 antibody at 10 μg/mL.)

WB (Western Blot) (Western blot analysis of BAP31 in rat lung tissue lysate with BAP31 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.)

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BAP31, Polyclonal Antibody (Cat# AAA39949)

Full Name

BAP31 Antibody

Gene Names

BCAP31; CDM; DDCH; BAP31; 6C6-AG; DXS1357E

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

BAP31 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.