Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of DRAM in mouse liver tissue with DRAM antibody at 2 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of DRAM in mouse liver tissue with DRAM antibody at 20 μg/mL.Red: DRAM Antibody (4035)Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of DRAM in Human Liver tissue with DRAM antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of DRAM in human liver tissue with DRAM antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of DRAM in 293 cell lysate with DRAM antibody at (A) 1 and (B) 2 μg/mL.)

Compare (5 max)

DRAM, Polyclonal Antibody (Cat# AAA40052)

Full Name

DRAM Antibody

Gene Names

DRAM1; DRAM

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

DRAM Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of SLC29A3 in rat colon muscle tissue with SLC29A3 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of SLC29A3 in rat colon tissue with SLC29A3 antibody at 5 μg/mL.)

WB (Western Blot) (SLC29A3 Antibody.)

Compare (5 max)

SLC29A3, Polyclonal Antibody (Cat# AAA40654)

Full Name

SLC29A3 Antibody

Gene Names

SLC29A3; ENT3; HJCD; PHID; HCLAP

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

SLC29A3 antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of RT4 cells using anti-NOP2 antibody (AAA127255).Overlay histogram showing RT4 cells stained with AAA127255 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOP2 Antibody (AAA127255, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of NOP2 using anti-NOP2 antibody (AAA127255) and anti-Beta Tubulin antibody (M01857-3).NOP2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-NOP2 Antibody (AAA127255) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of NOP2 using anti-NOP2 antibody (AAA127255).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human RT4 whole cell lysates,Lane 6: human 293T tissue lysates,Lane 7: human SIHA whole cell lyates,Lane 8: human U251 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP2 antigen affinity purified polyclonal antibody (#AAA127255) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOP2 at approximately 120 kDa. The expected band size for NOP2 is at 120 kDa.)

Compare (5 max)

NOP2, Polyclonal Antibody (Cat# AAA127255)

Full Name

Anti-NOP2 Antibody Picoband

Gene Names

NOP2; NOL1; p120; NSUN1; NOP120

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Nucleobindin 2 was detected in paraffin-embedded sections of rat gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry) (Nucleobindin 2 was detected in paraffin-embedded sections of mouse gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of Nucleobindin 2 expression in HEPA whole cell lysates (lane 1). Nucleobindin 2 at 55KD was detected using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

Compare (5 max)

Nucleobindin 2, Polyclonal Antibody (Cat# AAA46281)

Full Name

Anti-Nucleobindin 2 Antibody

Gene Names

Nucb2; Nefa; Calnuc; AI607786; Nesfatin-1

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Anti-TdT Picoband antibody, AAA46022-2.jpgAll lanes: Anti TDT (AAA46022) at 0.5ug/mlWB: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 58KD)

Application Data (Anti-TdT Picoband antibody, AAA46022-1.jpgAll lanes: Anti TDT (AAA46022) at 0.5ug/mlWB: Recombinant Human TDT Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)

Compare (5 max)

DNA nucleotidylexotransferase, Polyclonal Antibody (Cat# AAA46022)

Full Name

Anti-TdT Antibody

Gene Names

DNTT; TDT

Reactivity

Human. No cross reactivity with other proteins.

Applications

Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U2OS cells using anti-ACK1/TNK2 antibody (AAA126216).Overlay histogram showing U2OS cells stained with AAA126216 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACK1/TNK2 Antibody (AAA126216, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of ACK1/TNK2 using anti-ACK1/TNK2 antibody (AAA126216).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACK1/TNK2 antigen affinity purified polyclonal antibody (#AAA126216) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACK1/TNK2 at approximately 115 kDa. The expected band size for ACK1/TNK2 is at 115 kDa.)

Compare (5 max)

ACK1/TNK2, Polyclonal Antibody (Cat# AAA126216)

Full Name

Anti-ACK1/TNK2 Antibody Picoband

Gene Names

TNK2; ACK; ACK1; ACK-1; p21cdc42Hs

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HeLa cells using anti-MSH6 antibody (AAA126037).Overlay histogram showing HeLa cells stained with AAA126037 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSH6 Antibody (AAA126037, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of MSH6 using anti-MSH6 antibody (AAA126037).MSH6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-MSH6 Antibody (AAA126037) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of MSH6 using anti-MSH6 antibody (AAA126037).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH6 antigen affinity purified polyclonal antibody (#AAA126037) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MSH6 at approximately 160 kDa. The expected band size for MSH6 is at 160 kDa.)

Compare (5 max)

MSH6, Polyclonal Antibody (Cat# AAA126037)

Full Name

Anti-MSH6 Antibody Picoband

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of PC-3 cells using anti-Desmoplakin/DSP antibody (AAA126048).Overlay histogram showing PC-3 cells stained with AAA126048 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Desmoplakin/DSP Antibody (AAA126048, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of Desmoplakin/DSP using anti-Desmoplakin/DSP antibody (AAA126048).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoplakin/DSP antigen affinity purified polyclonal antibody (#AAA126048) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Desmoplakin/DSP at approximately 250 kDa. The expected band size for Desmoplakin/DSP is at 250 kDa.)

Compare (5 max)

Desmoplakin/DSP, Polyclonal Antibody (Cat# AAA126048)

Full Name

Anti-Desmoplakin/DSP Antibody Picoband

Gene Names

DSP; DP; DPI; DPII

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of JK cells using anti-ROR beta/RORB antibody (AAA126559).Overlay histogram showing JK cells stained with AAA126559 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR beta/RORB Antibody (AAA126559, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of ROR beta/RORB using anti-ROR beta/RORB antibody (AAA126559).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human placenta tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR beta/RORB antigen affinity purified polyclonal antibody (#AAA126559) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ROR beta/RORB at approximately 53 kDa. The expected band size for ROR beta/RORB is at 53 kDa.)

Compare (5 max)

ROR beta/RORB, Polyclonal Antibody (Cat# AAA126559)

Full Name

Anti-ROR beta/RORB Antibody Picoband

Gene Names

RORB; RZRB; NR1F2; ROR-BETA; RZR-BETA; bA133M9.1

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of HEPA1-6 cells using anti-IL-2 Receptor alpha/Il2ra antibody (AAA125983).Overlay histogram showing HEPA1-6 cells stained with AAA125983 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (AAA125983, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (AAA125983).IL-2 Receptor alpha/Il2ra was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (AAA125983) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (AAA125983).IL-2 Receptor alpha/Il2ra was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (AAA125983) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (AAA125983).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: mouse ANA-1 whole cell lysates,Lane 2: mouse NIH/3T3 whole cell lysates,Lane 3: mouse RAW264.7 whole cell lysates,Lane 4: mouse HEPA1-6 whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: rat NRK whole cell lysates,Lane 7: rat RH35 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 Receptor alpha/Il2ra antigen affinity purified polyclonal antibody (#AAA125983) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-2 Receptor alpha/Il2ra at approximately 50 kDa. The expected band size for IL-2 Receptor alpha/Il2ra is at 50 kDa.)

Compare (5 max)

IL-2 Receptor alpha/Il2ra, Polyclonal Antibody (Cat# AAA125983)

Full Name

Anti-IL-2 Receptor alpha/Il2ra Antibody Picoband

Gene Names

Il2ra; CD25; Il2r; Ly-43

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (HEK293 overexpressing BHMT RC203148) and probed (mock transfection in first lane), tested by Origene.)

WB (Western Blot) ((0. 03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Compare (5 max)

BHMT, Polyclonal Antibody (Cat# AAA61309)

Full Name

Goat anti-BHMT Antibody

Gene Names

BHMT; BHMT1; HEL-S-61p

Reactivity

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (LIN28A antibody (pAb) tested by Western blot. Cytosolic extract (40 ug) of mouse ES cells probed with LIN28A antibody at a dilution of 1:500.)

ICC (Immunocytochemistry) (LIN28A antibody (pAb) tested by Immunocytochemistry Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with LIN28A antibody at 1:200 dilution overnight at 4 degree C (left panel). Nuclei were stained with DAPI (right panel). Images show that LIN28A antibody specifically stains mESC colonies and does not stain MEFs. Absence of LIN28A staining in a subset of cells within the colonies suggests differentiation.)

Compare (5 max)

LIN28A, Polyclonal Antibody (Cat# AAA59992)

Full Name

LIN28A antibody (pAb)

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purified

Pricing

$250/0.01 mL $705/0.1 mL $1,375/2x0.1 mL $2,005/3x0.1 mL $2,605/4x0.1 mL $3,015/5x0.1 mL

IHC (Immunohistochemistry) ((4ug/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

Compare (5 max)

ERBB2/HER2, Polyclonal Antibody (Cat# AAA61726)

Full Name

Goat anti-ERBB2/HER2 (aa182-194) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Immunohistochemistry, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

< >

<< Previous Next >>

Application Data

IF (Immunofluorescence)

Compare (5 max)

NFkB-p65, Polyclonal Antibody (Cat# AAA47797)

Full Name

NFkB-p65 (Phospho-Ser468)

Gene Names

RELA; p65; NFKB3

Applications

Immunofluorescence, Western Blot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of BRCA1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of BRCA1 expression in HeLa (A), Mouse liver (B), Rat liver (C) whole cell lysates. (Predicted band size: 7; 78-85; 202-210 kD; Observed band size: 210 kD))

Compare (5 max)

BRCA1, Polyclonal Antibody (Cat# AAA105271)

Full Name

Anti-BRCA1 Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

< >

<< Previous Next >>

Application Data

Application Data

Compare (5 max)

USP38, Polyclonal Antibody (Cat# AAA112203)

Full Name

USP38 Antibody

Gene Names

USP38; HP43.8KD

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$440/0.1 mg $710/0.1 mL (AF568) $710/0.1 mL (AF555) $710/0.1 mL (AF488) $710/0.1 mL (AF350) $710/0.1 mL (FITC) $710/0.1 mL (Biotin) $710/0.1 mL (AF405M) $710/0.1 mL (AF635) $710/0.1 mL (AF610) $710/0.1 mL (AF405S) $710/0.1 mL (AF405L) $710/0.1 mL (AF546) $710/0.1 mL (AF680) $710/0.1 mL (AF647) $710/0.1 mL (AF594) $710/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AF750) $710/0.1 mL (AP)

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Fig: 2 Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem  (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.)

IHC (Immunohistochemistry) (Fig: 1 Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).)

Compare (5 max)

Caspase 8, Polyclonal Antibody (Cat# AAA78320)

Full Name

Polyclonal Antibody to Caspase 8 (active/cleaved)

Gene Names

Casp8; MACH; Mch5; FLICE; CASP-8

Reactivity

Dog, Gerbil, Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

$365/0.05 mL $705/2x0.05 mL $1,030/3x0.05 mL $1,335/4x0.05 mL $1,460/5x0.05 mL

Application Data

Compare (5 max)

Goat Anti-Human lambda (lambda chain specific), Polyclonal Secondary Antibody (Cat# AAA78804)

Full Name

Goat Anti-Human Lambda-UNLB

Reactivity

Human

Applications

Flow Cytometry

Purity

Affinity chromatography on human lambda light chains covalently linked to agarose

Pricing

$250/1 mg $490/2x1 mg $715/3x1 mg $930/4x1 mg $935/5x1 mg

< >

<< Previous Next >>

SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse kidney tissue, Mouse heart tissue, Primary antibody: AAA239214(NDUFS4 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA239214(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA239214(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))

Compare (5 max)

NDUFS4, Polyclonal Antibody (Cat# AAA239214)

Full Name

NDUFS4 Antibody

Gene Names

NDUFS4; AQDQ; CI-18

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

< >

<< Previous Next >>

SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: 293T cells, A549 cells, human testis tissue, mouse thymus tissue, Primary antibody: AAA239171(MRPL28 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA239171(MRPL28 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA239171(MRPL28 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))

Compare (5 max)

MRPL28, Polyclonal Antibody (Cat# AAA239171)

Full Name

MRPL28 Antibody

Gene Names

MRPL28; p15; MAAT1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA240942(NCAM1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using AAA240942(NCAM1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))

Compare (5 max)

NCAM1, Polyclonal Antibody (Cat# AAA240942)

Full Name

NCAM1 Antibody

Gene Names

NCAM1; CD56; NCAM; MSK39

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241508(SLC22A2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241508(SLC22A2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

Compare (5 max)

SLC22A2, Polyclonal Antibody (Cat# AAA241508)

Full Name

SLC22A2 Antibody

Gene Names

SLC22A2; OCT2

Reactivity

Human, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry of RTP801 in mouse kidney tissue with RTP801 antibody at 5 ug/mL.)

WB (Western Blot) (Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 ug/mL.)

Compare (5 max)

RTP801, Polyclonal Antibody (Cat# AAA302931)

Full Name

RTP801 Antibody

Gene Names

DDIT4; Dig2; REDD1; REDD-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/60.)

Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): Hela and A549 cell Amount of lysate: 30ug per lane Primary antibody: 1/1200 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)

Compare (5 max)

PAWR, Polyclonal Antibody (Cat# AAA302803)

Full Name

PAWR Antibody

Gene Names

PAWR; PAR4; Par-4

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with G-CSF 25ng/ml 15', using IL-3R beta (Phospho-Tyr593) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using IL-3R beta (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IL-3R beta, Polyclonal Antibody (Cat# AAA316152)

Full Name

IL-3R beta (Phospho-Tyr593) Antibody

Gene Names

CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAPKAPK5 (Phospho-Thr182) Antibody)

Compare (5 max)

MAPKAPK5, Polyclonal Antibody (Cat# AAA318353)

Full Name

MAPKAPK5 (Phospho-Thr182) Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from MCF-7, HUVEC, HeLa, and HepG2 cells, using OR2T3/2T34 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using OR2T3/2T34 Antibody. The picture on the right is blocked with the synthesized peptide.)

Compare (5 max)

OR2T3/2T34, Polyclonal Antibody (Cat# AAA318184)

Full Name

OR2T3/2T34 Antibody

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embeded human liver using at dilution of 1:100.)

WB (Western Blot)

Compare (5 max)

Endoplasmic reticulum resident protein 29, Polyclonal Antibody (Cat# AAA308418)

Full Name

Endoplasmic reticulum resident protein 29 Polyclonal Antibody

Gene Names

ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig

Applications

Immunohistochemistry, Western Blot

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$385/0.1 mg $745/2x0.1 mg $1,090/3x0.1 mg $1,410/4x0.1 mg $1,525/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (CDR2 Antibody (C-term) (AAA289107)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDR2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (CDR2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CDR2 antibody detected the CDR2 protein (arrow).)

Compare (5 max)

CDR2, Polyclonal Antibody (Cat# AAA289107)

Full Name

CDR2 Antibody (C-term)

Gene Names

CDR2; Yo; CDR62

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (KIF11 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of KIF11 Antibody (N-term) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.)

Compare (5 max)

KIF11, Polyclonal Antibody (Cat# AAA289541)

Full Name

KIF11 Antibody (N-term)

Gene Names

KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

WB (Western Blot) (ANGPT1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ANGPT1 antibody detected the ANGPT1 protein (arrow).)

WB (Western Blot) (Anti-ANGPT1 Antibody (C-term)at 1:2000 dilution + mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

ANGPT1, Polyclonal Antibody (Cat# AAA289560)

Full Name

ANGPT1 Antibody (C-term)

Gene Names

ANGPT1; AGP1; AGPT; ANG1

Reactivity

Human, mouse (Predicted Reactivity: Bovine, Mouse, Pig)

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human liver using at dilution of 1:100.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human small intestine using at dilution of 1:100.)

WB (Western Blot) (All lanes: Sorcin antibody at 2ug/ml)

Compare (5 max)

SRI, Polyclonal Antibody (Cat# AAA308983)

Full Name

SRI Polyclonal Antibody

Gene Names

SRI; SCN; V19; CP22; CP-22

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$385/0.1 mg $745/2x0.1 mg $1,090/3x0.1 mg $1,410/4x0.1 mg $1,525/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

KCNH4, Polyclonal Antibody (Cat# AAA319547)

Full Name

KCNH4 Antibody

Gene Names

KCNH4; BEC2; ELK1; Kv12.3

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$265/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (GLA/Alpha Galactosidase Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (GLA/Alpha Galactosidase Antibody-Western Blot; Sample: Recombinant GLa, Human.)

Compare (5 max)

GLA/Alpha Galactosidase, Polyclonal Antibody (Cat# AAA162757)

Full Name

GLA/Alpha Galactosidase Rabbit anti-Human Polyclonal (aa147-371) Antibody

Gene Names

GLA; GALA

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (WLS/GPR177 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (WLS/GPR177 Antibody-Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.)

Compare (5 max)

WLS/GPR177, Polyclonal Antibody (Cat# AAA162589)

Full Name

WLS/GPR177 Rabbit anti-Human Polyclonal (aa53-102) Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot of SOX9 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate at 3 ug/ml.)

WB (Western Blot) (Western blot of SOX9 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.)

IHC (Immunohiostchemistry) (Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (IHC of SOX9 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top left) (bottom left, right) at 5 ug/ml.)

Compare (5 max)

SOX9, Polyclonal Antibody (Cat# AAA162154)

Full Name

Anti-SOX9 Antibody (aa225-275) IHC-plus

Gene Names

SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10

Reactivity

Mouse, Dog, Rat, Human

Applications

Western Blot, Immunohistochemistry

Purity

Protein A purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human brain using MCTS1 Polyclonal Antibody at dilution of 1:60)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using MCTS1 Polyclonal Antibody at dilution of 1:60)

WB (Western Blot) (Western Blot analysis of Jurkat and PC3 cell using MCTS1 Polyclonal Antibody at dilution of 1:200)

Compare (5 max)

MCTS1, Polyclonal Antibody (Cat# AAA166764)

Full Name

MCTS1 Polyclonal Antibody

Gene Names

MCTS1; MCT1; MCT-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Figure 3. IHC analysis of DEPDC6/DEPTOR using anti-DEPDC6/DEPTOR antibody (AAA128211).DEPDC6/DEPTOR was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DEPDC6/DEPTOR Antibody (AAA128211) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of DEPDC6/DEPTOR using anti-DEPDC6/DEPTOR antibody (AAA128211).DEPDC6/DEPTOR was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DEPDC6/DEPTOR Antibody (AAA128211) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DEPDC6/DEPTOR using anti-DEPDC6/DEPTOR antibody (AAA128211).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human HEL whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat skeletal muscle tissue lysates,Lane 7: mouse heart tissue lysates,Lane 8: mouse skeletal muscle tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEPDC6/DEPTOR antigen affinity purified polyclonal antibody (AAA128211) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DEPDC6/DEPTOR at approximately 48 kDa. The expected band size for DEPDC6/DEPTOR is at 46 kDa.)

Compare (5 max)

DEPDC6/DEPTOR, Polyclonal Antibody (Cat# AAA128211)

Full Name

Anti-DEPDC6/DEPTOR Antibody Picoband

Gene Names

DEPTOR; DEP.6; DEPDC6

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326546 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using CAPZA2 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

CAPZA2, Polyclonal Antibody (Cat# AAA326546)

Full Name

CAPZA2 Antibody

Gene Names

CAPZA2; CAPZ; CAPPA2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA326547 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326547 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Jurkat whole cell lysates, using RUNX1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

RUNX1, Polyclonal Antibody (Cat# AAA326547)

Full Name

RUNX1 Antibody

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA326554 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of LoVo cell lysate, using CHUK Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326554 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Compare (5 max)

CHUK, Polyclonal Antibody (Cat# AAA326554)

Full Name

CHUK Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326558 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of MCF-7, using TOR1A antibody. The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

TOR1A, Polyclonal Antibody (Cat# AAA326558)

Full Name

TOR1A Antibody

Gene Names

TOR1A; DQ2; DYT1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326559 at 1/100 staining Human heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using E2F1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

E2F1, Polyclonal Antibody (Cat# AAA326559)

Full Name

E2F1 Antibody

Gene Names

E2F1; RBP3; E2F-1; RBAP1; RBBP3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of Jurkat, using FGFR1 antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326563 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326563 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

FGFR1, Polyclonal Antibody (Cat# AAA326563)

Full Name

FGFR1 Antibody

Gene Names

FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326564 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of HepG2 cell lysate, using FGFR2 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

FGFR2, Polyclonal Antibody (Cat# AAA326564)

Full Name

FGFR2 Antibody

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA326567 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of HEK-293, using GSK3A/B antibody. The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of GSK3B expression in various lysates)

Compare (5 max)

GSK3A/B, Polyclonal Antibody (Cat# AAA326567)

Full Name

GSK3A/B Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA326575 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of fetal pancreas, using INS antibody. The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

INS, Polyclonal Antibody (Cat# AAA326575)

Full Name

INS Antibody

Gene Names

INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.