Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-AR-S213 antibody.)

WB (Western Blot) (Western blot analysis of extracts from DU145 cells using Phospho-AR-S213 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

AR-S213, Antibody (Cat# AAA37346)

Full Name

Phospho-AR-S213 Polyclonal Antibody

Gene Names

AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-eIF2α-S49 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells, using phospho-EIF2S1-S49 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

EIF2S1-S49, Antibody (Cat# AAA37348)

Full Name

Phospho-EIF2S1-S49 Polyclonal Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-MARCKS-S158 antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells using Phospho-MARCKS-S158 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

MARCKS-S158, Antibody (Cat# AAA37368)

Full Name

Phospho-MARCKS-S158 Polyclonal Antibody

Gene Names

MARCKS; MACS; 80K-L; PKCSL; PRKCSL

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-RELA-S276 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-RELA-S276 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human breast using Phospho-RELA-S276 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat testis using Phospho-RELA-S276 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Phospho-RELA-S276 antibody. HeLa cells were treated by TNF-α (20ng/ml) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

RELA-S276, Antibody (Cat# AAA37383)

Full Name

Phospho-RELA-S276 Polyclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-HDAC8-S39 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung carcinoma using Phospho-HDAC8-S39 antibody.)

Compare (5 max)

HDAC8-S39, Antibody (Cat# AAA37390)

Full Name

Phospho-HDAC8-S39 Polyclonal Antibody

Gene Names

HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-NF2-S518 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells, using Phospho-NF2-S518 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

NF2-S518, Antibody (Cat# AAA37395)

Full Name

Phospho-NF2-S518 Polyclonal Antibody

Gene Names

NF2; ACN; SCH; BANF

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma, using Phospho-EIF4EBP1-T36 antibody.)

WB (Western Blot) (Western blot analysis of extracts from MDA-MB-435 cells, using Phospho-EIF4EBP1-T36 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

EIF4EBP1-T36, Antibody (Cat# AAA37397)

Full Name

Phospho-EIF4EBP1-T36 Polyclonal Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) at 37? for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25?g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.)

Compare (5 max)

SRC-Y416, Polyclonal Antibody (Cat# AAA37409)

Full Name

Phospho-SRC Family-Y416 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-CFL1-S3 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-CFL1-S3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CFL1-S3 antibody at 1:1000 dilution. NIH/3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.)

Compare (5 max)

CFL1-S3, Antibody (Cat# AAA37412)

Full Name

Phospho-CFL1-S3 Polyclonal Antibody

Gene Names

CFL1; CFL; HEL-S-15

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-STMN1-S38 antibody.)

WB (Western Blot) (Western blot analysis of extracts of K-562 cells, using Phospho-STMN1-S38 antibody. K562 cells were treated by serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Compare (5 max)

STMN1-S38, Antibody (Cat# AAA37418)

Full Name

Phospho-STMN1-S38 Polyclonal Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using ALOX5 (Phospho-Ser523) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using ALOX5 (Phospho-Ser523) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

ALOX5, Polyclonal Antibody (Cat# AAA243241)

Full Name

Phospho-ALOX5 (Ser523) Antibody

Gene Names

ALOX5; 5-LO; 5LPG; LOG5; 5-LOX

Reactivity

Human, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Mst1/2 (Phospho-Thr183) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1/2 (Phospho-Thr183) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

STK4, Polyclonal Antibody (Cat# AAA243255)

Full Name

Phospho-STK4 (Thr183) Antibody

Gene Names

STK4; KRS2; MST1; YSK3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Tau(Phospho-Ser262) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser262) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

MAPT, Polyclonal Antibody (Cat# AAA243277)

Full Name

Phospho-MAPT (Ser262) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-GR (Phospho-Ser226) Antibody.)

Application Data (HeLa cells were grown to 90% confluency and were stimulated with PMA 150 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of GR P-Ser226 versus untreated HeLa lysates.)

Compare (5 max)

GR, ELISA Kit (Cat# AAA318615)

Full Name

GR (Phospho-Ser226) Phospho Sandwich ELISA Kit

Gene Names

NR3C1; GR; GCR; GRL; GCCR; GCRST

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

< >

<< Previous Next >>

ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-FAK (Phospho-Tyr397) Antibody.)

Application Data (HeLa cells were grown to 90% confluency and stimulated with 30 ng/mL TNF-)

Compare (5 max)

FAK, ELISA Kit (Cat# AAA318617)

Full Name

FAK (Phospho-Tyr397) Phospho Sandwich ELISA Kit

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

< >

<< Previous Next >>

ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-DARPP-32 (Phospho-Thr34) Antibody.)

Application Data (HepG2 cells were grown to 90% confluency and were stimulated with PMA 150 ng/mL and incubated for 30 mins. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of DARPP-32 P-Thr34 versus untreated HepG2 lysates.)

Compare (5 max)

DARPP-32, ELISA Kit (Cat# AAA318622)

Full Name

DARPP-32 (Phospho-Thr34) Phospho Sandwich ELISA Kit

Gene Names

PPP1R1B; DARPP32; DARPP-32

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

< >

<< Previous Next >>

ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-Retinoblastoma (Phospho-Ser780) Antibody.)

Application Data (Hek 293 cells were grown to 90% confluency and were stimulated with 20% Serum and incubated for 1 hr. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Retinoblastoma P-Ser780 versus untreated Hek 293 lysates.)

Compare (5 max)

Retinoblastoma, ELISA Kit (Cat# AAA318628)

Full Name

Retinoblastoma (Phospho-Ser780) Phospho Sandwich ELISA Kit

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

< >

<< Previous Next >>

Application Data (HepG2 cells were grown to 90% confluency and were stimulated with Vanadate 1mM and incubated for 30 mins. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of DDR1 P-Tyr513 versus untreated HepG2 lysates.)

ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-DDR1 (Phospho-Tyr513) Antibody.)

Compare (5 max)

DDR1, ELISA Kit (Cat# AAA318631)

Full Name

DDR1 (Phospho-Tyr513) Phospho Sandwich ELISA Kit

Gene Names

DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

< >

<< Previous Next >>

Application Data (Gao, Ting-Ting, et al. "LIMK1/2 in the mPFC plays a role in chronic stress-induced depressive-like effects in mice." International Journal of Neuropsychopharmacology 23.12 (2020): 821-836.)

WB (Western Blot) (Western Blot analysis of various cells using Phospho-LIMK-1/2 (T508/505) Polyclonal Antibody)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using LIMK1/2 (Phospho-Thr508/505) Antibody. The picture on the right is blocked with the LIMK1/2 (Phospho-Thr508/505) peptide.)

Compare (5 max)

LIMK-1/2, Polyclonal Antibody (Cat# AAA318640)

Full Name

LIMK-1/2 (Phospho-Thr508/505) Rabbit Polyclonal Antibody

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT4 (Phospho-Tyr693) Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using STAT4 (Phospho-Tyr693) Antibody. The picture on the right is blocked with the STAT4 (Phospho-Tyr693) peptide.)

Compare (5 max)

Stat4, Polyclonal Antibody (Cat# AAA318641)

Full Name

Stat4 (Phospho-Tyr693) Rabbit Polyclonal Antibody

Gene Names

STAT4; SLEB11

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

Application Data (Li, Lin, et al. "Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2/M phase arrest via the p38 MAPK signaling pathway." Acta Pharmacologica Sinica 35.10 (2014): 1311.)

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cdc25A (S75) Polyclonal Antibody)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using CDC25A (Phospho-Ser75) Antibody. The picture on the right is blocked with the CDC25A (Phospho-Ser75) peptide.)

WB (Western Blot) (Western blot analysis of CDC25A (Phospho-Ser75) Antibody. The lane on the right is blocked with the CDC25A (Phospho-Ser75) peptide.)

Compare (5 max)

Cdc25A, Polyclonal Antibody (Cat# AAA318643)

Full Name

Cdc25A (Phospho-Ser75) Rabbit Polyclonal Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA, using p-Serynapsin I (Phospho-Ser553) antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synapsin I (Phospho-Ser553) Antibody)

Compare (5 max)

Synapsin I, Polyclonal Antibody (Cat# AAA318273)

Full Name

Synapsin I (Phospho-Ser553) Antibody

Gene Names

SYN1; SYNI; SYN1a; SYN1b

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from rat brain, using CaMK2-beta/gamma/delta (Phospho-Thr287) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2-beta/gamma/delta (Phospho-Thr287) Antibody)

Compare (5 max)

CaMK2-beta/gamma/delta, Polyclonal Antibody (Cat# AAA318293)

Full Name

CaMK2-beta/gamma/delta (Phospho-Thr287) Antibody

Gene Names

CAMK2B; CAM2; CAMK2; CAMKB; MRD54

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with UV 15', using IGF2R (Phospho-Ser2409) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IGF2R (Phospho-Ser2409) Antibody)

Compare (5 max)

IGF2R, Polyclonal Antibody (Cat# AAA318328)

Full Name

IGF2R (Phospho-Ser2409) Antibody

Gene Names

IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody)

Compare (5 max)

RSK1/2/3/4, Polyclonal Antibody (Cat# AAA318382)

Full Name

RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using STMN1 (Phospho-Ser62) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STMN1 (Phospho-Ser62) Antibody)

Compare (5 max)

STMN1, Polyclonal Antibody (Cat# AAA318391)

Full Name

STMN1 (Phospho-Ser62) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody)

Compare (5 max)

IKK-alpha, Polyclonal Antibody (Cat# AAA318329)

Full Name

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr192) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Lck (Phospho-Tyr192) Antibody)

Compare (5 max)

Lck, Polyclonal Antibody (Cat# AAA318344)

Full Name

Lck (Phospho-Tyr192) Antibody

Gene Names

LCK; LSK; YT16; IMD22; p56lck; pp58lck

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

WB (Western Blot) (Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

NDRG1, ELISA Kit (Cat# AAA319085)

Full Name

NDRG1 (Phospho-Thr346) Colorimetric Cell-Based ELISA Kit

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

Bim, ELISA Kit (Cat# AAA319095)

Full Name

Bim (Phospho-Ser77) Colorimetric Cell-Based ELISA Kit

Gene Names

BCL2L11; BAM; BIM; BOD

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

Connexin 43, ELISA Kit (Cat# AAA319101)

Full Name

Connexin 43 (Phospho-Tyr265) Colorimetric Cell-Based ELISA Kit

Gene Names

GJA1; HSS; CX43; GJAL; ODDD; AVSD3; HLHS1; DFNB38

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

NPM, ELISA Kit (Cat# AAA319115)

Full Name

NPM (Phospho-Thr95) Colorimetric Cell-Based ELISA Kit

Gene Names

NPM1; B23; NPM

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

SEK1/MKK4, ELISA Kit (Cat# AAA319122)

Full Name

SEK1/MKK4 (Phospho-Ser257/Thr261) Colorimetric Cell-Based ELISA Kit

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

SEK1/MKK4, ELISA Kit (Cat# AAA319124)

Full Name

SEK1/MKK4 (Phospho-Thr261) Colorimetric Cell-Based ELISA Kit

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

Mcl-1, ELISA Kit (Cat# AAA319137)

Full Name

Mcl-1 (Phospho-Ser159/Thr163) Colorimetric Cell-Based ELISA Kit

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-Trk C (Y516) Polyclonal Antibody)

Compare (5 max)

Trk C, ELISA Kit (Cat# AAA319140)

Full Name

Trk C (Phospho-Tyr516) Colorimetric Cell-Based ELISA Kit

Gene Names

NTRK3; TRKC; gp145(trkC)

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

Integrin beta2, ELISA Kit (Cat# AAA319143)

Full Name

Integrin beta2 (Phospho-Thr758) Colorimetric Cell-Based ELISA Kit

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-FGFR-4 (Y642) Polyclonal Antibody)

Compare (5 max)

FGFR-4, ELISA Kit (Cat# AAA319148)

Full Name

FGFR-4 (Phospho-Tyr642) Colorimetric Cell-Based ELISA Kit

Gene Names

FGFR4; TKF; JTK2; CD334

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

AMPKalpha1, ELISA Kit (Cat# AAA319158)

Full Name

AMPKalpha1 (Phospho-Ser182) Colorimetric Cell-Based ELISA Kit

Gene Names

PRKAB1; AMPK; HAMPKb

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of extracts from K562 cells, using Phospho-Ob-R (Y1141) Polyclonal Antibody.)

Compare (5 max)

Ob-R, ELISA Kit (Cat# AAA319183)

Full Name

Ob-R (Phospho-Tyr1141) Colorimetric Cell-Based ELISA Kit

Gene Names

LEPR; OBR; OB-R; CD295; LEP-R; LEPRD

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of 293 using p-Plk (T210) antibody.)

Compare (5 max)

Plk, ELISA Kit (Cat# AAA319190)

Full Name

Plk (Phospho-Thr210) Colorimetric Cell-Based ELISA Kit

Gene Names

PLK1; PLK; STPK13

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1:1000)

Compare (5 max)

Raf-1, ELISA Kit (Cat# AAA319192)

Full Name

Raf-1 (Phospho-Ser301) Colorimetric Cell-Based ELISA Kit

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of 293 using p-c-Fms (Y809) antibody.)

Compare (5 max)

c-Fms, ELISA Kit (Cat# AAA319216)

Full Name

c-Fms (Phospho-Tyr809) Colorimetric Cell-Based ELISA Kit

Gene Names

CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of CoLo cells using Phospho-CREB-2 (S219) Polyclonal Antibody)

Compare (5 max)

CREB-2, ELISA Kit (Cat# AAA319217)

Full Name

CREB-2 (Phospho-Ser219) Colorimetric Cell-Based ELISA Kit

Gene Names

ATF4; CREB2; TXREB; CREB-2; TAXREB67

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using Cyclin C (Phospho-Ser275) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

Cyclin C, ELISA Kit (Cat# AAA319220)

Full Name

Cyclin C (Phospho-Ser275) Colorimetric Cell-Based ELISA Kit

Gene Names

CCNC; CycC

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with IFN 2500U/ML 30', using LCK (Phospho-Ser59) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

Lck, ELISA Kit (Cat# AAA319250)

Full Name

Lck (Phospho-Ser540) Colorimetric Cell-Based ELISA Kit

Gene Names

LCK; LSK; YT16; p56lck; pp58lck

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

MKK7, Polyclonal Antibody (Cat# AAA319462)

Full Name

MKK7 (Phospho-Ser271/Thr275) Antibody

Gene Names

MAP2K7; MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

SEK1/MKK4, Polyclonal Antibody (Cat# AAA319466)

Full Name

SEK1/MKK4 (Phospho-Ser80) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

LAT, Polyclonal Antibody (Cat# AAA319468)

Full Name

LAT (Phospho-Tyr171) Antibody

Gene Names

LAT; LAT1; pp36

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

MOB1, Polyclonal Antibody (Cat# AAA319476)

Full Name

MOB1 (Phospho-Thr35) Antibody

Gene Names

MOB1A; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs