Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of p53 phosphorylation expression in ovary cancer whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA321468 at 1/200 staining human kidney sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321468 staining MDA-MB-435 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Compare (5 max)

p53, Polyclonal Antibody (Cat# AAA321468)

Full Name

Phospho-p53 (Ser315) Antibody

Reactivity

Human

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Phospho-CTDSPL2 (Ser104) Antibody.)

IHC (Immunohistochemistry) (AAA321317 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

CTDSPL2, Polyclonal Antibody (Cat# AAA321317)

Full Name

Phospho-CTDSPL2 (Ser104) Antibody

Gene Names

CTDSPL2; HSPC058; HSPC129

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of MKN-45 cells treated with Forskolin, using Phospho-DARPP-32 (Ser97) Antibody.)

IHC (Immunohistochemistry) (AAA321320 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

DARPP-32, Polyclonal Antibody (Cat# AAA321320)

Full Name

Phospho-DARPP-32 (Ser97) Antibody

Gene Names

PPP1R1B; DARPP32; DARPP-32

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA321321 staining Heat-shock treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)

WB (Western Blot) (Western blot analysis of extracts of T47-D cells treated with collagen, using Phospho-DDR1 (Tyr513) Antibody.)

IHC (Immunohistochemistry) (AAA321321 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

DDR1, Polyclonal Antibody (Cat# AAA321321)

Full Name

Phospho-DDR1 (Tyr513) Antibody

Gene Names

DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of serum-starved HeLa cells, using Phospho-eEF2k (Ser366) Antibody.)

IHC (Immunohistochemistry) (AAA321324 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

eEF2k, Polyclonal Antibody (Cat# AAA321324)

Full Name

Phospho-eEF2k (Ser366) Antibody

Gene Names

EEF2K; eEF-2K; CaMKIII; HSU93850

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of PC12 cells, using Phospho-eIF4G (Ser1147) Antibody.)

IHC (Immunohistochemistry) (AAA321325 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

eIF4G, Polyclonal Antibody (Cat# AAA321325)

Full Name

Phospho-eIF4G (Ser1147) Antibody

Gene Names

EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent analysis of Histone H3 (pS10) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of Histone H3 (pS10) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Histone H3 (pS10) expression in HEK293T LPS-treated (A), Raw264.7 H2O2-treated (B), PC12 H2O2-treated (C) whole cell lysates.)

Compare (5 max)

Histone H3 (pS10), Polyclonal Antibody (Cat# AAA105161)

Full Name

Anti-Histone H3 (pS10) Phospho Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human cervix cancer, using Phospho-JAK2 (Y1007 + Y1008) Antibody.)

IF (Immunofluorescence) (Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using Phospho-JAK2 (Y1007 + Y1008) Antibody.)

WB (Western Blot) (Western blot analysis of JAK2 phosphorylation expression in Jurkat cell lysates treated with Pervanadate.)

Compare (5 max)

JAK2, Monoclonal Antibody (Cat# AAA125166)

Full Name

Anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal Antibody

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

Affinity-Chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-PAK1-pT423 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-PAK1-pT423 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Compare (5 max)

Phospho-PAK1 (T423), Polyclonal Antibody (Cat# AAA288458)

Full Name

Phospho-PAK1 (T423) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat, Rabbit, Xenopus)

Applications

Dot Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

Application Data (The TFact STAT5B (Phospho-Ser731) DNA-Binding ELISA detects active STAT5B (Phospho-Ser731) in Hela and HepG2 Nuclear Extract. The Hela and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

Compare (5 max)

STAT5B, ELISA Kit (Cat# AAA315994)

Full Name

STAT5B (Phospho-Ser731) TFact DNA-Binding ELISA Kit

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using MARCKS (Phospho-Ser158) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MARCKS (Phospho-Ser158) Antibody)

Compare (5 max)

MARCKS, Polyclonal Antibody (Cat# AAA318354)

Full Name

MARCKS (Phospho-Ser158) Antibody

Gene Names

MARCKS; MACS; 80K-L; PKCSL; PRKCSL

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

WB (Western Blot) (Western Blot analysis of various cells using Phospho-PI 3-kinase p85alpha (Y607) Polyclonal Antibody diluted at 1:1000)

Compare (5 max)

PI 3-kinase p85alpha, ELISA Kit (Cat# AAA319189)

Full Name

PI 3-kinase p85alpha (Phospho-Tyr607) Colorimetric Cell-Based ELISA Kit

Gene Names

PIK3R1; p85; AGM7; GRB1; p85-ALPHA

Reactivity

Human, Mouse, Rat, Chicken (tested by customer)

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using VE-Cadherin (Phospho-Tyr731) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

VE-Cadherin, ELISA Kit (Cat# AAA319201)

Full Name

VE-Cadherin (Phospho-Tyr731) Colorimetric Cell-Based ELISA Kit

Gene Names

CDH5; 7B4; CD144

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-p16 (S326) Polyclonal Antibody diluted at 1:500)

Compare (5 max)

p16-INK4a, ELISA Kit (Cat# AAA319204)

Full Name

p16-INK4a (Phospho-Ser152) Colorimetric Cell-Based ELISA Kit

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of FosB (Phospho-Ser27) Antibody. The lane on the right is blocked with the FosB (Phospho-Ser27) peptide.)

Compare (5 max)

Fos B, ELISA Kit (Cat# AAA319233)

Full Name

Fos B (Phospho-Ser27) Colorimetric Cell-Based ELISA Kit

Gene Names

FOSB; AP-1; G0S3; GOS3; GOSB

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of COLO cells using Phospho-JIP-1 (T103) Polyclonal Antibody)

Compare (5 max)

JIP-1, ELISA Kit (Cat# AAA319245)

Full Name

JIP-1 (Phospho-Thr103) Colorimetric Cell-Based ELISA Kit

Gene Names

MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

YB1, ELISA Kit (Cat# AAA319092)

Full Name

YB1 (Phospho-Ser102) Colorimetric Cell-Based ELISA Kit

Gene Names

TPSAB1; TPS1; TPS2; TPSB1

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

TCTP, ELISA Kit (Cat# AAA319110)

Full Name

TCTP (Phospho-Ser46) Colorimetric Cell-Based ELISA Kit

Gene Names

TPT1; HRF; p02; TCTP

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of Maize 1) Dark, 2) Light, diluted at 1:2000. Secondary antibody was diluted at 1:20000)

Compare (5 max)

PEPC, ELISA Kit (Cat# AAA319120)

Full Name

PEPC (Phospho-Ser15) Colorimetric Cell-Based ELISA Kit

Gene Names

PGC; PEPC; PGII

Reactivity

Plants

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using UBF (Phospho-Ser484) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

UBF, ELISA Kit (Cat# AAA319129)

Full Name

UBF (Phospho-Ser484) Colorimetric Cell-Based ELISA Kit

Gene Names

UBTF; UBF; UBF1; UBF-1; NOR-90

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree C over night)

Compare (5 max)

Stathmin, ELISA Kit (Cat# AAA319134)

Full Name

Stathmin (Phospho-Ser16) Colorimetric Cell-Based ELISA Kit

Gene Names

STMN2; SCG10; SCGN10

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

CD28, ELISA Kit (Cat# AAA319141)

Full Name

CD28 (Phospho-Tyr218) Colorimetric Cell-Based ELISA Kit

Gene Names

CD28; Tp44

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with UV 15', using IGF2R (Phospho-Ser2409) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

IGF-IIR, ELISA Kit (Cat# AAA319142)

Full Name

IGF-IIR (Phospho-Ser2409) Colorimetric Cell-Based ELISA Kit

Gene Names

IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R

Reactivity

Human, Mouse, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of 293T VEC lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000)

Compare (5 max)

PRC1, ELISA Kit (Cat# AAA319151)

Full Name

PRC1 (Phospho-Thr481) Colorimetric Cell-Based ELISA Kit

Gene Names

PRC1; ASE1

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using CHOP (Phospho-Ser30) Antibody. The lane on the right is blocked with the phospho peptide.)

Compare (5 max)

CHOP, ELISA Kit (Cat# AAA319163)

Full Name

CHOP (Phospho-Ser30) Colorimetric Cell-Based ELISA Kit

Gene Names

DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

SEK1/MKK4, Polyclonal Antibody (Cat# AAA319467)

Full Name

SEK1/MKK4 (Phospho-Thr261) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

< >

<< Previous Next >>

Application Data (Diluted at 1:10000)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

Compare (5 max)

Kinesin-like Protein KIF1C, Polyclonal Antibody (Cat# AAA319470)

Full Name

Kinesin-like Protein KIF1C (Phospho-Ser1092) Antibody

Gene Names

KIF1C; SAX2; LTXS1; SATX2; SPAX2; SPG58

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

WB (Western Blot) (Western Blot analysis of A431 cells using Phospho-PKD1 (S910) Polyclonal Antibody)

Compare (5 max)

PKD1, ELISA Kit (Cat# AAA319274)

Full Name

PKD1 (Phospho-Ser910) Colorimetric Cell-Based ELISA Kit

Gene Names

PRKD1; PKD; PKCM; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-TPH2 (S19) Polyclonal Antibody)

Compare (5 max)

TPH2, ELISA Kit (Cat# AAA319281)

Full Name

TPH2 (Phospho-Ser19) Colorimetric Cell-Based ELISA Kit

Gene Names

TPH2; NTPH; ADHD7

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA320723 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis on K562 cell lysate using Phospho-VEGFR2(Tyr1214) Antibody.The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

VEGFR2, Polyclonal Antibody (Cat# AAA320723)

Full Name

Phospho-VEGFR2(Tyr1214) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

IHC (Immunohistochemistry) (AAA320729 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4 degree C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

Compare (5 max)

EPHA2/3/4, Polyclonal Antibody (Cat# AAA320729)

Full Name

Phospho-EPHA2/3/4(Tyr588/596) Antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA320735 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA320735 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on HepG2 cell lysate using Phospho-CD227/MUC1(Tyr1229) Antibody.The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

CD227/MUC1, Antibody (Cat# AAA320735)

Full Name

Phospho-CD227/MUC1(Tyr1229) Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (AAA320738 at 1/200 staining human Cerebellum tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis on rat heart cell lysate using Phospho-PAK3(Ser154) Antibody.The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

PAK3, Polyclonal Antibody (Cat# AAA320738)

Full Name

Phospho-PAK3(Ser154) Antibody

Gene Names

PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA320744 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA320744 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on K562 cell lysate using Phospho-MAPKAPK5(Thr182) Antibody, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

MAPKAPK5, Polyclonal Antibody (Cat# AAA320744)

Full Name

Phospho-MAPKAPK5(Thr182) Antibody

Gene Names

MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA322067 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322067 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of eIF4B (Phospho-Ser422) expression in HepG2 cells.The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

eIF4B, Polyclonal Antibody (Cat# AAA322067)

Full Name

eIF4B (Phospho-Ser422) Antibody

Gene Names

EIF4B; EIF-4B; PRO1843

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA322069 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA322069 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2, using IKK-gamma (Phospho-Ser85) Antibody.)

Compare (5 max)

IKK-gamma, Polyclonal Antibody (Cat# AAA322069)

Full Name

IKK-gamma (Phospho-Ser85) Antibody

Gene Names

IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA322072 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Cyclin C (Phospho-Ser275) expression in LOVO cells.The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Cyclin C, Polyclonal Antibody (Cat# AAA322072)

Full Name

Cyclin C (Phospho-Ser275) Antibody

Gene Names

CCNC; CycC; SRB11; hSRB11

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA322075 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA322075 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA322075 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of hnRPD (Phospho-Ser83) expression in 293 cell extract.The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

hnRPD, Polyclonal Antibody (Cat# AAA322075)

Full Name

hnRPD (Phospho-Ser83) Antibody

Gene Names

HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA322076 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322076 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of ANXA2 (Phospho-Ser26) Antibody expression in Hela cells lysates.)

Compare (5 max)

ANXA2, Polyclonal Antibody (Cat# AAA322076)

Full Name

ANXA2 (Phospho-Ser26) Antibody

Gene Names

ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (AAA322077 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Daxx (Phospho-Ser668) Antibody expression in 293 cells lysates.The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA322077 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Compare (5 max)

Daxx, Polyclonal Antibody (Cat# AAA322077)

Full Name

Daxx (Phospho-Ser668) Antibody

Gene Names

DAXX; DAP6; EAP1; BING2

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Pin1 (Ser16) Antibody expression in Insulin treated COS7 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321720 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Pin1 phosphorylation expression in Insulin treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321720 at 1/200 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

Pin1, Polyclonal Antibody (Cat# AAA321720)

Full Name

Phospho-Pin1 (Ser16) Antibody

Gene Names

PIN1; DOD; UBL5

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA321721 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-4E-BP1 (Thr36) expression in various lysates)

WB (Western Blot) (Western blot analysis of 4E-BP1 phosphorylation expression in EGF treated MDA-MB-435 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321721 at 1/100 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

4E-BP1, Polyclonal Antibody (Cat# AAA321721)

Full Name

Phospho-4E-BP1 (Thr36) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Kv2.1 (Ser805) Antibody expression in TNF treated K562 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Kv2.1 phosphorylation expression in TNF treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Kv2.1, Polyclonal Antibody (Cat# AAA321730)

Full Name

Phospho-Kv2.1 (Ser805) Antibody

Gene Names

KCNB1; DRK1; Kv2.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Smad2 (Ser467) expression in various lysates)

WB (Western Blot) (Western blot analysis of extracts from rat brain, mouse muscle, using Phospho-Smad2 (Ser467) Antibody.)

IHC (Immunohistochemisry) (AAA321738 at 1/100 staining human kidney carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Smad2 phosphorylation expression in PMA treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321738 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Compare (5 max)

Smad2, Polyclonal Antibody (Cat# AAA321738)

Full Name

Phospho-Smad2 (Ser467) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Smad2 (Ser250) Antibody expression in PMA treated 293 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Smad2 phosphorylation expression in PMA treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321739 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Compare (5 max)

Smad2, Polyclonal Antibody (Cat# AAA321739)

Full Name

Phospho-Smad2 (Ser250) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Trk B (Tyr705) expression in various lysates)

IF (Immunofluorescence) (AAA321749 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IF (Immunofluorescence) (AAA321749 staining NIH/3T3 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA321749 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Trk B phosphorylation expression in Mouse kidneytissue lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Trk B, Polyclonal Antibody (Cat# AAA321749)

Full Name

Phospho-Trk B (Tyr705) Antibody

Gene Names

NTRK2; OBHD; TRKB; trk-B; EIEE58; GP145-TrkB

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of FRS2 phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (Phospho-FRS2 (Tyr436) Antibody for IHC in human brain tissue)

WB (Western Blot) (Western blot analysis of FRS2 phosphorylation expression in Hela whole cell lysates, The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

FRS2, Polyclonal Antibody (Cat# AAA321754)

Full Name

Phospho-FRS2 (Tyr436) Antibody

Gene Names

FRS2; SNT; SNT1; FRS1A; FRS2A; SNT-1; FRS2alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-BLNK (Tyr96) Antibody expression in COLO205 cells lysates.The lane on the right was treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321755 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of BLNK phosphorylation expression in COLO205 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321755 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-BLNK (Tyr96) Antibody expression in COLO205 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

BLNK, Polyclonal Antibody (Cat# AAA321755)

Full Name

Phospho-BLNK (Tyr96) Antibody

Gene Names

BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA321763 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA321763 staining HT-1080 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohistochemisry) (AAA321763 at 1/100 staining human Bone marrow tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of AurB phosphorylation expression in Nocodazole treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-AurB (Thr232) Antibody expression in Nocodazole treated COS7 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

AurB, Polyclonal Antibody (Cat# AAA321763)

Full Name

Phospho-AurB (Thr232) Antibody

Gene Names

AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-p62 Dok (Tyr362) expression in various lysates)

WB (Western Blot) (Western blot analysis of p62 Dok phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321766 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Compare (5 max)

p62 Dok, Polyclonal Antibody (Cat# AAA321766)

Full Name

Phospho-p62 Dok (Tyr362) Antibody

Gene Names

DOK1; pp62; P62DOK

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs