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Application
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345 results for " Cytoplasmic" - showing 300-345
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NRK Antibody (Center) (AAA28689). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NRK, Polyclonal Antibody (Cat# AAA28689)
Full Name
NRK Antibody (Center)
Gene Names
NRK; NESK
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31186 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31186 1:200) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Cytoplasmic dynein 1 intermediate chain 1, Polyclonal Antibody (Cat# AAA31186)
Full Name
Cytoplasmic dynein 1 intermediate chain 1 Antibody
Gene Names
DYNC1I1; DNCI1; DNCIC1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ATF6, Polyclonal Antibody (Cat# AAA31376)
Full Name
Phospho-ATF6 (Thr166) Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Horse (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of PFA-fixed HePG2 cells using Tumor Necrosis Factor (TNF alpha) Mouse Monoclonal Antibody (TNF706) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.)
TNF-alpha (Tumor Necrosis Factor alpha), Monoclonal Antibody (Cat# AAA13806)
Full Name
TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody
Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Reactivity
Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2466). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Ki-67, Monoclonal Antibody (Cat# AAA23909)
Full Name
Ki-67 (Proliferating Cell Marker)
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12085)
Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)
CD3, Monoclonal Antibody (Cat# AAA13839)
Full Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human, Mouse, Rat
Applications
Functional Assay, Flow Cytometry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(AAA31064 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PKC theta, Polyclonal Antibody (Cat# AAA31064)
Full Name
Phospho-PKC theta (Ser676) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
CSK, Polyclonal Antibody (Cat# AAA31403)
Full Name
Phospho-CSK (Tyr304) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12083)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA11865)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12029)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
SAA (Serum Amyloid A), Polyclonal Antibody (Cat# AAA14452)
Full Name
Affinity Purified Goat anti-Human SAA Antibody
Gene Names
SAA1; SAA
Reactivity
Human
Purity
Unconjugated A.P.
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
ITGAM/CD11B, Polyclonal Antibody (Cat# AAA13654)
Full Name
Goat anti-ITGAM/CD11B Antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Tested: Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Applications
Peptide ELISA, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))
Cytokeratin 19 (KRT19), Monoclonal Antibody (Cat# AAA13824)
Full Name
Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Mouse Monoclonal Antibody
Gene Names
KRT19; K19; CK19; K1CS
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
Doublecortin/DCX, Polyclonal Antibody (Cat# AAA13668)
Full Name
Goat anti-Doublecortin/DCX (aa232-242) Antibody
Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Species Reactivity: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(AAA30992 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SP1, Polyclonal Antibody (Cat# AAA30992)
Full Name
Phospho-SP1 (Thr739) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31127 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31127 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.The nuclear counter stain is DAPI(blue).)
PRDX6, Polyclonal Antibody (Cat# AAA31127)
Full Name
PRDX6 Antibody
Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#AAA28756), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA28756)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.)
RIPK3, Polyclonal Antibody (Cat# AAA23385)
Full Name
RIPK3 antibody - N-terminal region
Gene Names
RIPK3; RIP3
Reactivity
Tested Species Reactivty: Human
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(IHC of Parvovirus B19 on a FFPE Infected Placenta Tissue)
Parvovirus, Monoclonal Antibody (Cat# AAA13563)
Full Name
Parvovirus
Reactivity
Human
Applications
Immunohistochemistry
Pricing
Application Data
MMP-9, Polyclonal Antibody (Cat# AAA14085)
Full Name
Rabbit anti MMP-9 Polyclonal antibody
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Human, Rat, Mouse, Chicken, Dog
Applications
Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Pricing
ICC (Immunocytochemistry)
(ICC staining DYNLL1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
DYNLL1, Monoclonal Antibody (Cat# AAA30208)
Full Name
DYNLL1 Antibody
Gene Names
DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(IHC: Human ovary carcinoma stained with Anti-Legumain (AAA14072) 1:200 dilution. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Legumain, Polyclonal Antibody (Cat# AAA14072)
Full Name
Rabbit anti Legumain Polyclonal Antibody
Gene Names
LGMN; AEP; LGMN1; PRSC1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Epitope Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of MeOH-fixed MCF-7 cells using Cytokeratin 19 Monoclonal Antibody followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Cytokeratin 19 (KRT19), Monoclonal Antibody (Cat# AAA13816)
Full Name
Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Mouse Monoclonal Antibody
Gene Names
KRT19; K19; CK19; K1CS
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
IS (Immunostaining)
(Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
Androgen Receptor, Polyclonal Antibody (Cat# AAA13656)
Full Name
Goat anti-Androgen Receptor Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(AAA30991 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.)
SP1, Polyclonal Antibody (Cat# AAA30991)
Full Name
Phospho-SP1 (Thr453) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30935 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GR, Polyclonal Antibody (Cat# AAA30935)
Full Name
Phospho-GR (Ser211) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(RGS1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RGS1, Polyclonal Antibody (Cat# AAA28739)
Full Name
RGS1 Antibody (N-term)
Gene Names
RGS1; 1R20; BL34; IER1; IR20; HEL-S-87
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(AAA13670 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NRF2, Polyclonal Antibody (Cat# AAA13670)
Full Name
Goat anti-NRF2 (aa445-458) Antibody
Gene Names
NFE2L2; NRF2; HEBP1
Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Western Blot Analysis of Human Kidney lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).)
Calnexin, Monoclonal Antibody (Cat# AAA23894)
Full Name
Calnexin (Endoplasmic Reticulum Marker)
Gene Names
CANX; CNX; P90; IP90
Reactivity
Human.
Does not react with rat. Others not known.
Does not react with rat. Others not known.
Applications
Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(KRAS Antibody (C-term)(AAA28748) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
KRAS, Polyclonal Antibody (Cat# AAA28748)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat (Predicted: Rat)
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(AAA13649 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Arrestin beta 2, Antibody (Cat# AAA13649)
Full Name
Goat anti-Arrestin beta 2 Antibody
Gene Names
ARRB2; ARB2; ARR2; BARR2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Western Blot Analysis of human kidney cell lysate using Ferritin, Light ChainMouse Monoclonal Antibody (FTL/1387).)
Ferritin, Light Chain (FTL), Monoclonal Antibody (Cat# AAA23907)
Full Name
Ferritin, Light Chain (FTL) (Microglia Marker)
Gene Names
FTL; LFTD; NBIA3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: Caco2 whole cell lysateLane 2: mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (please inquire) at 1/15000 dilution.Observed band size : 55kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
DHCR7, Polyclonal Antibody (Cat# AAA28753)
Full Name
DHCR7 Antibody (C-term)
Gene Names
DHCR7; SLOS
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
Cadherin 17/LI Cadherin, Monoclonal Antibody (Cat# AAA23911)
Full Name
Cadherin 17/LI Cadherin (Liver-Intestine Marker)
Gene Names
CDH17; HPT1; CDH16; HPT-1
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Pricing
FCM (Flow Cytometry)
(SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SCNN1A, Polyclonal Antibody (Cat# AAA28784)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HeLa cells stained with AAA28782(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28782, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
HNRNPR, Polyclonal Antibody (Cat# AAA28782)
Full Name
HNRNPR Antibody (N-term)
Gene Names
HNRNPR; HNRPR; hnRNP-R
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry: Human carcinoma (FFPE) stained with Rabbit anti-TGF at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
GSK-3b (pY216), Antibody (Cat# AAA14095)
Full Name
Rabbit anti Phospho- GSK-3beta (pTyr 216)
Reactivity
Human, Rat, Mouse, Bovine, Chicken
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
AIF1/IBA1, Polyclonal Antibody (Cat# AAA13659)
Full Name
Goat anti-AIF1/IBA1 (isoform 3) Antibody
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog
Expected from sequence similarity: Human, Mouse, Dog
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
SLC26A3, Polyclonal Antibody (Cat# AAA23387)
Full Name
SLC26A3 antibody - middle region
Gene Names
SLC26A3; CLD; DRA
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.)
S100A4, Monoclonal Antibody (Cat# AAA23892)
Full Name
S100A4 (Marker of Tumor Metastasis)
Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)
Full Name
Laminin Receptor/RPSA (Marker of Metastatic Potential)
Gene Names
RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing