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Type
Reactivity
Gene Name
Isotype
Host
Application
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345 results for " Cytoplasmic" - showing 250-300
WB (Western Blot)
(Figure 9 WB Validation of CXCR4 in metastatic melanoma (Scala et al., 2006)CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.)
CXCR4, Polyclonal Antibody (Cat# AAA10962)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
CXCR4 Antibody is Protein A purified.
Pricing
WB (Western Blot)
(Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 20 μg per lane. Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
CXCR4, Polyclonal Antibody (Cat# AAA10951)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
CXCR4 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
Tryptase, Monoclonal Antibody (Cat# AAA13565)
Full Name
Tryptase
Gene Names
MCT7; PMCT7; TPSB1
Reactivity
Human
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Recommended IHC Protocol)
CD68, Monoclonal Antibody (Cat# AAA13569)
Full Name
CD68
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human, Hamster, Mouse, Non-Human Primate, Porcine, Rabbit, Rat (Reactivity: Paraffin, Frozen)
Applications
Immunohistochemistry
Pricing
IF (Immunofluorescence)
(AAA31187 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31187 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Syntrophin alpha 1, Polyclonal Antibody (Cat# AAA31187)
Full Name
Syntrophin alpha 1 Antibody
Gene Names
SNTA1; SNT1; LQT12; TACIP1; dJ1187J4.5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit(88%), Dog(88%)
Predicted Reactivity: Rabbit(88%), Dog(88%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
SDS-PAGE
(SDS-PAGE Analysis of Purified SM-MHC Monoclonal Antibody (SMMS-1). Confirmation of Integrity and Purity of Antibody)
Smooth Muscle Myosin Heavy Chain (SM-MHC), Monoclonal Antibody (Cat# AAA13842)
Full Name
Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Mouse Monoclonal Antibody
Gene Names
MYH11; AAT4; FAA4; SMHC; SMMHC
Reactivity
Cat, Chicken, Cow, Dog, Guinea Pig, Human, Pig, Rabbit, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(IHC of an FFPE Breast Tissue)
CA15-3, Monoclonal Antibody (Cat# AAA13555)
Full Name
CA15-3
Reactivity
Paraffin, frozen
Applications
Immunohistochemistry, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)
Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)
Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Predicted Species Reactivity: Rat
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
FAK, Polyclonal Antibody (Cat# AAA31446)
Full Name
Phospho-FAK (Ser722) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Anti-c-Met antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
c-Met, Monoclonal Antibody (Cat# AAA12343)
Full Name
Mouse Monoclonal (IgG2a) to Human c-Met
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified Podocalyxin Mouse Monoclonal Antibody (3D3). Confirmation of Integrity and Purity of Antibody.)
Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA13811)
Full Name
Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal Antibody
Gene Names
PODXL; PC; PCLP; Gp200; PCLP-1
Reactivity
Human, Rabbit, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (SPM194).)
Blood Group Antigen Lewis B, Monoclonal Antibody (Cat# AAA13833)
Full Name
Blood Group Antigen Lewis B Mouse Monoclonal Antibody
Gene Names
FUT3; LE; Les; FT3B; CD174; FucT-III
Reactivity
Human, Guinea Pig
Applications
Immunofluorescence, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENOA, Polyclonal Antibody (Cat# AAA28805)
Full Name
ENOA Antibody (N-term)
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Monkey
Predicted: Bovine, Monkey
Applications
Immunofluorescence, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclnal Antibody (Pab)
Pricing
SDS-PAGE
(SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Preformed Fibrils. Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils)
Tau441, Active Protein (Cat# AAA27663)
Full Name
Active Human Recombinant Tau441 (2N4R), P301S Mutant Protein Preformed Fibrils
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Applications
Western Blot
Purity
>95%
Ion-Exchange Purified
Ion-Exchange Purified
Pricing
FCM (Flow Cytometry)
(LTF Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (lefthistogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for theanalysis.)
LTF, Polyclonal Antibody (Cat# AAA28680)
Full Name
LTF Antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Mammary Cancer Tissue)
beta-Actin, Monoclonal Antibody (Cat# AAA11508)
Full Name
Anti-beta-Actin antibody (monoclonal)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(WB of AAA14403 with 1:500 antibody dilution in DiluObuffer. Apparent MW: 48)
FtsZ, Polyclonal Antibody (Cat# AAA14403)
Full Name
FtsZ Antibody
Applications
Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistchemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
c-FOS, Antibody (Cat# AAA13673)
Full Name
Goat anti-c-FOS (aa283-295) Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
Isoleucine--tRNA ligase, cytoplasmic (Iars), Recombinant Protein (Cat# AAA18749)
Full Name
Recombinant Mouse Isoleucine--tRNA ligase, cytoplasmic (Iars) , partial
Gene Names
Iars; ILRS; AI327140; AU044614; 2510016L12Rik; E430001P04Rik
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed K562 cells using Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1/832) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Calponin-1, Monoclonal Antibody (Cat# AAA13848)
Full Name
Calponin-1 (Smooth Muscle Marker)
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596).Confirmation of Integrity and Purity of Antibody)
GFAP, Monoclonal Antibody (Cat# AAA13808)
Full Name
GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target), Monoclonal Antibody (Cat# AAA23916)
Full Name
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target)
Gene Names
ICOSLG; B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified PTH Mouse Monoclonal Antibody (3H9). Confirmation of Purity and Integrity of Antibody)
Parathyroid Hormone (PTH), Monoclonal Antibody (Cat# AAA13828)
Full Name
Parathyroid Hormone (PTH) (N-Terminal) Mouse Monoclonal Antibody
Gene Names
PTH; PTH1
Reactivity
Human.
Predicted to react with Mouse, Rat, Rabbit, Cow, Dog, Pig, Deer and Orangutan
Predicted to react with Mouse, Rat, Rabbit, Cow, Dog, Pig, Deer and Orangutan
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)
NANOG, Polyclonal Antibody (Cat# AAA28706)
Full Name
NANOG Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(ICH of Macrophage HAM-56 on an FFPE Liver Tissue)
Macrophage HAM-56, Monoclonal Antibody (Cat# AAA13562)
Full Name
Macrophage HAM-56
Reactivity
Human, Monkey
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(FOXD1 Antibody (N-term) (AAA28675) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FOXD1, Polyclonal Antibody (Cat# AAA28675)
Full Name
FOXD1 Antibody (N-term)
Gene Names
FOXD1; FKHL8; FREAC4; FREAC-4
Reactivity
Human, Mouse; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of human Raji cells using HLA-DR MAb (SPM423) followed by Goat anti-mouse I G-CF488 (Blue); Isotype Control (Red).)
HLA-DRB, Monoclonal Antibody (Cat# AAA13826)
Full Name
HLA-DRB (MHC II) Mouse Monoclonal Antibody
Gene Names
HLA-DRB1; SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B
Reactivity
Human, Monkey
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(AAA31079 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MCL1, Polyclonal Antibody (Cat# AAA31079)
Full Name
MCL1 Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Staining Procedure
(1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol.)
Podoplanin/D2-40, Monoclonal Antibody (Cat# AAA13558)
Full Name
Podoplanin/D2-40
Reactivity
Human, Rat, Mouse
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using AAA14660 and following by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.)
Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14660)
Full Name
Mast Cell Chymase (BSA & Azide Free)
Gene Names
CMA1; CYH; MCT1; chymase; MGC119890; MGC119891
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Protein G affinity chromatography.
Pricing
Application Data
(Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) in tau K18 P301L monomers over time. Thioflavin T ex = 450 nm, em = 485 nm.)
Tau, Active Protein (Cat# AAA27662)
Full Name
Active Human Recombinant Tau (K18), P301L Mutant Protein Monomer
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Applications
Western Blot
Purity
Purity: >95%
Purification: Ion-exchange Purified
Purification: Ion-exchange Purified
Pricing
S100A9, Monoclonal Antibody (Cat# AAA13837)
Full Name
S100A9 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human and Rat. Others not tested.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry: Lung tissue (FFPE) stained with Rabbit anti-ER81 (Cat# AAA14073) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
ER81, Polyclonal Antibody (Cat# AAA14073)
Full Name
Rabbit anti ER81 Polyclonal Antibody
Gene Names
ETV1; ER81
Reactivity
Mouse, Human, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Rabbit IgG is purified by Epitope Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Monoclonal Antibody (SPM225).)
VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA13827)
Full Name
VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Mouse, Rat, Rabbit, Dog. Others not known.
Applications
Immunofluorescence, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(AAA13648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
AKAP9/AKAP450/CG-NAP, Polyclonal Antibody (Cat# AAA13648)
Full Name
Goat anti-AKAP9/AKAP450/CG-NAP Antibody
Gene Names
AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Applications
Peptide ELISA, Western Blot, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA31292)
Full Name
Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody
Gene Names
EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
Actin, Smooth Muscle, Antibody (Cat# AAA14079)
Full Name
Rabbit anti SMA antibody (Smooth Muscle Actin)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The Rabbit IgG is purified by Epitope affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution)
MGEA5, Monoclonal Antibody (Cat# AAA30518)
Full Name
MGEA5 Antibody
Gene Names
MGEA5; OGA; MEA5; NCOAT
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Affinity-chromatography
Pricing
ICC (Immunocytochemistry)
(Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to (green). Nuclei are stained with Red dot)
CD68, Antibody (Cat# AAA13807)
Full Name
CD68 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human, Monkey, Mouse, and Rat. Others not known.
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(AAA31050 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZAP-70, Polyclonal Antibody (Cat# AAA31050)
Full Name
Phospho-ZAP-70 (Tyr319) Antibody
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30968 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GR, Polyclonal Antibody (Cat# AAA30968)
Full Name
Phospho-GR (Ser226) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Smad3 phosphorylation expression in HT29 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Smad3, Polyclonal Antibody (Cat# AAA31058)
Full Name
Phospho-Smad3 (Ser213) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12084)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(AAA31434 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31434 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Smad3, Polyclonal Antibody (Cat# AAA31434)
Full Name
Phospho-Smad3 (Ser423+Ser425) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
IRAK4 (pT345), Monoclonal Antibody (Cat# AAA14096)
Full Name
Mouse anti Phospho-IRAK-4 (pThr345)
Gene Names
IRAK4; IPD1; REN64; NY-REN-64
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The Mouse IgG is purified by Isotyping-specific Affinity Purification.
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
ACO1, Recombinant Protein (Cat# AAA11797)
Full Name
ACO1, 1-889aa, Human, His tag, E Coli
Gene Names
ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Western blot analysis of eIF2 alpha phosphorylation expression in IFN-alpha treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
eIF2 alpha, Polyclonal Antibody (Cat# AAA30978)
Full Name
Phospho-eIF2 alpha (Ser51) Antibody
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
BRK, Polyclonal Antibody (Cat# AAA31371)
Full Name
Phospho-BRK (Tyr447) Antibody
Gene Names
PTK6; BRK
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (89%)
Predicted Reactivity: Horse (89%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
TCR Beta F1, Monoclonal Antibody (Cat# AAA13568)
Full Name
TCR Beta F1
Reactivity
Human
Applications
Immunohistochemistry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA11933)
Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing