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298 results for " Actin" - showing 200-250
WB (Western Blot)
(ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)
ACTN4, Monoclonal Antibody (Cat# AAA25598)
Full Name
ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (PE)
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)
SRC, Monoclonal Antibody (Cat# AAA28639)
Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
WB (Western Blot)
(WB Suggested Anti-ACTR1B Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateACTR1B is supported by BioGPS gene expression data to be expressed in HepG2)
ACTR1B, Polyclonal Antibody (Cat# AAA23545)
Full Name
ACTR1B antibody - C-terminal region
Gene Names
ACTR1B; PC3; ARP1B; CTRN2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in PC-12.)
CFL1, Monoclonal Antibody (Cat# AAA24464)
Full Name
CFL1 (Cofilin-1, Cofilin, Non-muscle Isoform, 18kD Phosphoprotein, p18, CFL) APC
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, Mouse
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
DB (Dot Blot)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Phospho-HIST1H3B3 (S10), Polyclonal Antibody (Cat# AAA28670)
Full Name
Phospho-HIST1H3B3 (S10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
DB, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (AAA19291).Overlay histogram showing U937 cells stained with AAA19291 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (AAA19291, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)
Full Name
Anti-Coronin 1a/TACO/CORO1A Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence Analysis of PFA fixed U87 cells labeling VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617) followed by Goat anti-mouse IgG-CF488 (Green).)
Vinculin, Monoclonal Antibody (Cat# AAA23953)
Full Name
Vinculin (Marker of Age-related Macular Degeneration)
Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish, Bird
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Standard Curve (Sample)
WB (Western Blot)
(ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)
ACTN4, Monoclonal Antibody (Cat# AAA25009)
Full Name
ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (FITC)
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)
SSH3, Monoclonal Antibody (Cat# AAA25264)
Full Name
SSH3 (Protein Phosphatase Slingshot Homolog 3, SSH-like Protein 3, SSH3L, SSH-3L, hSSH-3L) (FITC)
Gene Names
SSH3; SSH3L
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)
ACTN4, Monoclonal Antibody (Cat# AAA25305)
Full Name
ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (HRP)
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Standard Curve (Sample)
alpha Smooth Muscle Actin, ELISA Kit (Cat# AAA17056)
Full Name
Porcine alpha Smooth Muscle Actin ELISA Kit
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Porcine
Pricing
WB (Western Blot)
(Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
RPS6, Monoclonal Antibody (Cat# AAA28643)
Full Name
RPS6 Antibody (N-term)
Gene Names
RPS6; S6
Reactivity
Human, rat (Predicted Reactivity: Monkey, Mouse)
Applications
EIA, IHC, WB, FC/FACS, IF
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
Standard Curve (Sample)
actin, beta, ELISA Kit (Cat# AAA15353)
Full Name
Human beta-actin ELISA Kit
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human
Pricing
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
TESK2, Monoclonal Antibody (Cat# AAA26315)
Full Name
TESK2 (Testis-Specific Kinase 2) (FITC)
Applications
IF, IHC, WB
Purity
Purified
Pricing
WB (Western Blot)
(DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)
DAAM1, Monoclonal Antibody (Cat# AAA14782)
Full Name
DAAM1 (Disheveled-associated Activator Of Morphogenesis 1, KIAA0666)
Reactivity
Human, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
TESK2, Monoclonal Antibody (Cat# AAA26048)
Full Name
TESK2 (Testis-Specific Kinase 2) (APC)
Applications
IF, IHC, WB
Purity
Purified
Pricing
WB (Western Blot)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)
ACTB, Monoclonal Antibody (Cat# AAA25304)
Full Name
ACTB (Actin, Cytoplasmic 1, Beta-actin) (HRP)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)
ACTN4, Monoclonal Antibody (Cat# AAA24712)
Full Name
ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (Biotin)
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Standard Curve (Sample)
Filamin B, beta, ELISA Kit (Cat# AAA16590)
Full Name
Human Filamin B, beta ELISA Kit
Gene Names
FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
Reactivity
Human
Pricing
IHC (Immunohistchemistry)
(PINK1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
PINK1, Monoclonal Antibody (Cat# AAA28638)
Full Name
PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
WB (Western Blot)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)
ACTB, Monoclonal Antibody (Cat# AAA25008)
Full Name
ACTB (Actin, Cytoplasmic 1, Beta-actin) (FITC)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)
SSH3, Monoclonal Antibody (Cat# AAA24968)
Full Name
SSH3 (Protein Phosphatase Slingshot Homolog 3, SSH-like Protein 3, SSH3L, SSH-3L, hSSH-3L) (Biotin)
Gene Names
SSH3; SSH3L
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence Analysis of PFA-fixed U87MG cells stained using CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).)
CD63, Monoclonal Antibody (Cat# AAA13803)
Full Name
CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Figure 12 Immunohistochemistry Validation of PD-L1in Human Tumors (Gadiot et al., 2011)Immunohistochemical analysis of patient tumors labeling PD-L1 with anti-PD-L1 antibodies (AAA10941). Several anti-PD-L1 antibodies were tested for staining, “Only 1 antibody gave no background staining and was competitively blocked by the addition of PD-L1Fc protein (AAA10941)”.)
PDL-1, Polyclonal Antibody (Cat# AAA10941)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)
NPTX2, Polyclonal Antibody (Cat# AAA10933)
Full Name
NPTX2 Antibody
Gene Names
NPTX2; NP2; NARP; NP-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
NPTX2 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Figure 11 Immunofluorescence Validation of ACE2 In Caco2 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10945 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)
ACE2, Polyclonal Antibody (Cat# AAA10945)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
Actin, beta Human, Active Protein (Cat# AAA14745)
Full Name
Actin, beta Human
Gene Names
ACTB; BRWS1; PS1TP5BP1
Applications
EL/EIA, WB
Purity
>99%. May contain traces of gamma-actin.
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
H3K79me2, Antibody (Cat# AAA10648)
Full Name
Histone H3K79me2 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Figure 9 WB Validation of CXCR4 in metastatic melanoma (Scala et al., 2006)CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.)
CXCR4, Polyclonal Antibody (Cat# AAA10962)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
CXCR4 Antibody is Protein A purified.
Pricing
WB (Western Blot)
(Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 20 μg per lane. Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
CXCR4, Polyclonal Antibody (Cat# AAA10951)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
CXCR4 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Figure 10 KD Validation of BACE in DRG (Hyun, 2007)Decreased BACE1 expression in DRG following siRNA3 transfection. DRG neurons were transfected with 1 ug siRNA3 plasmid and incubated for 48 hours in 37°˚C. DRG neurons were stained for BACE1 us¬ing the Anti-BACE antibody (a,b) Neurons transfected with the control plas¬mid pSUPER-EGFP (green) did not display any changes in BACE1 expression (red). (c,d) DRG neurons transfected with siR¬NA3 displayed reduced BACE1 expression in the axon.)
BACE, Polyclonal Antibody (Cat# AAA10918)
Full Name
BACE Antibody
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
BACE Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immuofluorescence)
(Figure 9 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10928 at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)
ACE2, Polyclonal Antibody (Cat# AAA10928)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(AAA31240 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31240) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Anillin, Polyclonal Antibody (Cat# AAA31240)
Full Name
Anillin Antibody
Gene Names
ANLN; scra; FSGS8; Scraps
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)
Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)
Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Predicted Species Reactivity: Rat
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Cyclin D1, Polyclonal Antibody (Cat# AAA28781)
Full Name
Cyclin D1 Antibody (S90)
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENOA, Polyclonal Antibody (Cat# AAA28805)
Full Name
ENOA Antibody (N-term)
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Monkey
Predicted: Bovine, Monkey
Applications
Immunofluorescence, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclnal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Figure 8 Immunofluorescence Validation of JMJD3 in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde fixed K562 cells labeling JMJD3 with AAA10925 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
JMJD3, Antibody (Cat# AAA10925)
Full Name
JMJD3 Antibody
Gene Names
KDM6B; JMJD3
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
JMJD3 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12264)
Full Name
Mouse Anti Human CD169
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
FCM (Flow Cytometry)
(MUSK Antibody (AAA28697) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
MUSK, Polyclonal Antibody (Cat# AAA28697)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Mammary Cancer Tissue)
beta-Actin, Monoclonal Antibody (Cat# AAA11508)
Full Name
Anti-beta-Actin antibody (monoclonal)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
LC3, Polyclonal Antibody (Cat# AAA28778)
Full Name
LC3 Antibody (APG8B) (N-term)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed K562 cells using Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1/832) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Calponin-1, Monoclonal Antibody (Cat# AAA13848)
Full Name
Calponin-1 (Smooth Muscle Marker)
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Fig.7. Immunohistochemical analysis of paraffin-embedded rat kidney tissue.1, alpha-SMA Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min).3, secondary antibody was diluted at 1:200 (room temperature, 30min).Negative control was used by secondary antibody only.)
alpha-SMA, Monoclonal Antibody (Cat# AAA31477)
Full Name
alpha-SMA Monoclonal Antibody
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)
NANOG, Polyclonal Antibody (Cat# AAA28706)
Full Name
NANOG Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12197)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(FOXD1 Antibody (N-term) (AAA28675) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FOXD1, Polyclonal Antibody (Cat# AAA28675)
Full Name
FOXD1 Antibody (N-term)
Gene Names
FOXD1; FKHL8; FREAC4; FREAC-4
Reactivity
Human, Mouse; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12193)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Mammary cancer Tissue)
Gelsolin, Polyclonal Antibody (Cat# AAA20947)
Full Name
Polyclonal Antibody to Gelsolin (GS)
Gene Names
GSN; ADF; AGEL
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing