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Gene Name
Isotype
Host
Application
Clone
2304 results for " secondary" - showing 1850-1900
IF (Immunofluorescence)
(AAA31181 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31181 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Chromogranin C, Polyclonal Antibody (Cat# AAA31181)
Full Name
Chromogranin C Antibody
Gene Names
SCG2; SN; CHGC; EM66; SgII
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27703)
Full Name
VPS35 Antibody, Clone 11H10: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IF (Immunofluorescence)
(AAA31212 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31212 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAPP A2, Polyclonal Antibody (Cat# AAA31212)
Full Name
PAPP A2 Antibody
Gene Names
PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Sheep(80%)
Predicted Reactivity: Sheep(80%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31226 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31226 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SLFN11, Polyclonal Antibody (Cat# AAA31226)
Full Name
SLFN11 Antibody
Gene Names
SLFN11; SLFN8/9
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31000 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Tau, Polyclonal Antibody (Cat# AAA31000)
Full Name
Phospho-Tau (Ser422) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31233 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31233 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Guanylyl Cyclase alpha 2, Polyclonal Antibody (Cat# AAA31233)
Full Name
Guanylyl Cyclase alpha 2 Antibody
Gene Names
GUCY1A2; GC-SA2; GUC1A2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(100%), Rabbit(100%)
Predicted Reactivity: Horse(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31247 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31247) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MBD4, Polyclonal Antibody (Cat# AAA31247)
Full Name
MBD4 Antibody
Gene Names
MBD4; MED1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%)
Predicted Reactivity: Pig(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31264 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31264 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
RECQL5, Polyclonal Antibody (Cat# AAA31264)
Full Name
RECQL5 Antibody
Gene Names
RECQL5; RECQ5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(86%)
Predicted Reactivity: Pig(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31199 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31199 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
DPP9, Polyclonal Antibody (Cat# AAA31199)
Full Name
DPP9 - Catalytic domain Antibody
Gene Names
DPP9; DP9; DPLP9; DPRP2; DPP IX; DPRP-2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry
Purity
Peptide affintiy purification
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31163 at 1/100 staining Mouse pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NG2, Polyclonal Antibody (Cat# AAA31163)
Full Name
NG2 Antibody
Gene Names
CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31164 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31164 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Pericentrin - Centrosome Marker, Polyclonal Antibody (Cat# AAA31164)
Full Name
Pericentrin - Centrosome Marker Antibody
Gene Names
PCNT; KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PPIG, Polyclonal Antibody (Cat# AAA31370)
Full Name
Phospho-PPIG (Ser376) Antibody
Gene Names
PPIG; CYP; SRCyp; SCAF10; CARS-Cyp
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CB2, Polyclonal Antibody (Cat# AAA28672)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31081 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary Ab was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary Ab.)
Tyrosinase, Polyclonal Antibody (Cat# AAA31081)
Full Name
Tyrosinase Antibody
Gene Names
TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-p70 S6 Kinase (Ser371) expression in various lysates)
p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31024)
Full Name
Phospho-p70 S6 Kinase (Ser371) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31050 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZAP-70, Polyclonal Antibody (Cat# AAA31050)
Full Name
Phospho-ZAP-70 (Tyr319) Antibody
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-PKC-pan (Thr497) expression in various lysates)
PKC-pan, Polyclonal Antibody (Cat# AAA31017)
Full Name
Phospho-PKC-pan (Thr497) Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31382 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA31382 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NF kappaB p105/p50, Polyclonal Antibody (Cat# AAA31382)
Full Name
Phospho-NF kappaB p105/p50 (Ser923) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(Anti-NR6A1 / GCNF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
NR6A1 / GCNF, Polyclonal Antibody (Cat# AAA12361)
Full Name
Rabbit Polyclonal to Human NR6A1 / GCNF
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of Smad3 phosphorylation expression in HT29 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Smad3, Polyclonal Antibody (Cat# AAA31058)
Full Name
Phospho-Smad3 (Ser213) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(The involvement of AMPK and Akt in the negative regulation of mTOR by APPL1 in STZ-induced diabetic rats.The variations of p-AMPK and p-Akt in normal control rats with vacant lentiviral vector control, normal control rats with APPL1 knockdown, PDN rats, and PDN rats with APPL1 knockdown, and PDN rats with APPL1 overexpression.The abbreviations for the groups of negative control, normal control + shRNA encoding APPL1, painful diabetic neuropathy (PDN), PDN + shRNA encoding APPLL (shRNA) and PDN + APPL1 genetic overexpression are shown as CON, CON + shRNA, PDN, PDN + shRNA, and PDN + OXP. (n = 4, *P < 0.05 vs. NC group, # P < 0.05 vs. PDN group).Data are expressed as the means ± SEM.)
Phospho-AMPK1 (Ser486), Polyclonal Antibody (Cat# AAA31070)
Full Name
Phospho-AMPK1 (Ser486) Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using Phospho-Src (Tyr529) Antibody.)
Src, Polyclonal Antibody (Cat# AAA31009)
Full Name
Phospho-Src (Tyr530) Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of RH35 cells using anti-Podoplanin/gp36/Pdpn antibody (AAA19233).Overlay histogram showing RH35 cells stained with AAA19233 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Podoplanin/gp36/Pdpn Antibody (AAA19233, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Podoplanin/gp36/Pdpn, Polyclonal Antibody (Cat# AAA19233)
Full Name
Anti-Podoplanin/gp36/Pdpn Antibody
Gene Names
Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of n-NOS phosphorylation expression in A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
n-NOS, Polyclonal Antibody (Cat# AAA31028)
Full Name
Phospho-n-NOS (Ser852) Antibody
Gene Names
NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3086) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
MSH6, Monoclonal Antibody (Cat# AAA23917)
Full Name
MSH6 (DNA Mismatch Repair Protein)
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IRAK1, Polyclonal Antibody (Cat# AAA31367)
Full Name
Phospho-IRAK1 (Thr100) Antibody
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31434 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31434 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Smad3, Polyclonal Antibody (Cat# AAA31434)
Full Name
Phospho-Smad3 (Ser423+Ser425) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12188)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using AAA29763 (ISM1 Antibody) at dilution 1/60.(Original magnification: 200))
ISM1, Polyclonal Antibody (Cat# AAA29763)
Full Name
ISM1 Antibody
Gene Names
ISM1; ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: speB antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 44 kDaobserved band size: 44 kDa)
speB, Polyclonal Antibody (Cat# AAA26985)
Full Name
speB Antibody
Reactivity
Streptococcus pyogenes
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
WB (Western Blot)
(Western blot analysis of eIF2 alpha phosphorylation expression in IFN-alpha treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
eIF2 alpha, Polyclonal Antibody (Cat# AAA30978)
Full Name
Phospho-eIF2 alpha (Ser51) Antibody
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Leu-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.Images: Immunohistochemical detection of Leu-Enkephalin in rat ventral periaqueductal grey matter (PAG). Brain was fixed with 4% formaldehyde and cut into 10 ?m thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leu-Enkephalin at 10 ?g/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red (pseudo-coloured yellow) conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.)
Leu-Enkephalin, Antibody (Cat# AAA14441)
Full Name
Leu-Enkephalin, Affinity Purified Antibody, Rabbit
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PMS1 antibody (AAA28270) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
PMS1, Polyclonal Antibody (Cat# AAA28270)
Full Name
PMS1 Polyclonal Antibody
Gene Names
PMS1; MLH2; PMSL1; hPMS1; HNPCC3
Applications
Western Blot
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/106 cells of CD73 clone (ABM40E2). Green represents isotype control; red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
CD73, Monoclonal Antibody (Cat# AAA14868)
Full Name
CD73 Monoclonal Antibody
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Protein G Chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using SNRNP70 antibody (AAA28160) at dilution of 1:100 (40x lens).)
SNRNP70, Polyclonal Antibody (Cat# AAA28160)
Full Name
SNRNP70 Polyclonal Antibody
Gene Names
SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Intracellular FLOW cytometric analysis of Caspase 3 (Clone : ABM1C12) inon Jurkat cells using 05 µg/10^6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control, red represents anti-caspase 3 antibody.)
Caspase-3 (Pro and Active), Monoclonal Antibody (Cat# AAA14867)
Full Name
Caspase-3 (Pro and Active) Monoclonal Antibody
Gene Names
Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
Reactivity
Human
Applications
Westen Blot, Immunohistochemistry
Purity
Protein G Chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
DNA Polymerase zeta, Polyclonal Antibody (Cat# AAA27811)
Full Name
Anti-DNA Polymerase zeta Antibody
Gene Names
REV3L; POLZ; REV3
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Wilms Tumor Protein, Monoclonal Antibody (Cat# AAA30123)
Full Name
Wilms Tumor Protein Antibody
Gene Names
WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Affinity-chromatography
Pricing
IF (Immunofluorescence)
(AAA31280 staining A549 cells(H2O2 treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA31280) and mouse anti-beta tubulin Ab(#) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
PYCARD, Polyclonal Antibody (Cat# AAA31280)
Full Name
Phospho-PYCARD (Ser195) Antibody
Gene Names
PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31228 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Proprotein Convertase 2, Polyclonal Antibody (Cat# AAA31228)
Full Name
Proprotein Convertase 2 Antibody
Gene Names
PCSK2; PC2; NEC2; SPC2; NEC 2; NEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31268 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PRMT4, Polyclonal Antibody (Cat# AAA31268)
Full Name
PRMT4 Antibody
Gene Names
CARM1; PRMT4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA31461 at 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
MCM2, Polyclonal Antibody (Cat# AAA31461)
Full Name
Phospho-MCM2 (Ser53) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with AAA11732 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black))
PARP2, Monoclonal Antibody (Cat# AAA11732)
Full Name
PARP2 antibody
Gene Names
PARP2; ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(AAA31100 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)
DSS1, Polyclonal Antibody (Cat# AAA31100)
Full Name
DSS1 Antibody
Gene Names
SEM1; ECD; DSS1; SHFD1; SHFM1; SHSF1; Shfdg1; C7orf76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
MCM3, Monoclonal Antibody (Cat# AAA30464)
Full Name
MCM3 Antibody
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(AAA31112 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
OSR2, Polyclonal Antibody (Cat# AAA31112)
Full Name
OSR2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(WB Suggested Anti-ACTN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate)
ACTN1, Polyclonal Antibody (Cat# AAA23579)
Full Name
ACTN1 antibody - N-terminal region
Gene Names
ACTN1; BDPLT15
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(AAA31170 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31170 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Clathrin heavy chain, Polyclonal Antibody (Cat# AAA31170)
Full Name
Clathrin heavy chain Antibody
Gene Names
CLTCL1; CLTD; CHC22; CLH22; CLTCL
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish(88%), Rabbit(88%), Dog(100%), Chicken(88%)
Predicted Reactivity: Zebrafish(88%), Rabbit(88%), Dog(100%), Chicken(88%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Connexin 43 (Ser367) expression in various lysates)
Connexin 43, Polyclonal Antibody (Cat# AAA31018)
Full Name
Phospho-Connexin 43 (Ser367) Antibody
Gene Names
GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31166 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31166 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SC35, Polyclonal Antibody (Cat# AAA31166)
Full Name
SC35 Antibody
Gene Names
SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Predicted Reactivity: Pig(100%), Bovine(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing