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Reactivity

Gene Name

Isotype

Host

Application

Clone

2304 results for " secondary" - showing 1750-1800

product-image-AAA28304_IHC13.jpg
product-image-AAA28304_IHC13.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40xlens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

TOM20, Monoclonal Antibody (Cat# AAA28304)

Full Name
TOM20 Rabbit mAb
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Affinity purification
Pricing
product-image-AAA30117_FCM7.jpg
product-image-AAA30117_FCM7.jpg FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Prion Protein (PrP), Monoclonal Antibody (Cat# AAA30117)

Full Name
Prion Protein (PrP) Antibody
Gene Names
PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
product-image-AAA31201_IHC8.jpg
product-image-AAA31201_IHC8.jpg IHC (Immunohistochemistry-Paraffin) (AAA31201 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PICALM, Polyclonal Antibody (Cat# AAA31201)

Full Name
PICALM Antibody
Gene Names
PICALM; LAP; CALM; CLTH
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31022_IHC20.jpg
product-image-AAA31022_IHC20.jpg IHC (Immunohistochemistry) (AAA31022 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Filamin A, Polyclonal Antibody (Cat# AAA31022)

Full Name
Phospho-Filamin A (Ser2152) Antibody
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31253_IF7.jpg
product-image-AAA31253_IF7.jpg IF (Immunofluorescence) (AAA31253 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31253 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Connexin 40/GJA5, Polyclonal Antibody (Cat# AAA31253)

Full Name
Connexin 40/GJA5 Antibody
Gene Names
GJA5; CX40; ATFB11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31252_IHC6.jpg
product-image-AAA31252_IHC6.jpg IHC (Immunohistchemistry-Paraffin) (AAA31252 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

KDM5C/Jarid1C/SMCX, Polyclonal Antibody (Cat# AAA31252)

Full Name
KDM5C/Jarid1C/SMCX Antibody
Gene Names
KDM5C; MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31189_IF6.jpg
product-image-AAA31189_IF6.jpg IF (Immunofluorescence) (AAA31189 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31189 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Lgi1, Polyclonal Antibody (Cat# AAA31189)

Full Name
Lgi1 Antibody
Gene Names
LGI1; EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31195_IF6.jpg
product-image-AAA31195_IF6.jpg IF (Immunofluorescence) (AAA31195 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31195 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

P2X3, Polyclonal Antibody (Cat# AAA31195)

Full Name
P2X3 Antibody
Gene Names
P2RX3; P2X3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31116_IF3.jpg
product-image-AAA31116_IF3.jpg IF (Immunofluorescence) (AAA31116 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31116 1:200) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)

PLCB2, Polyclonal Antibody (Cat# AAA31116)

Full Name
PLCB2 Antibody
Gene Names
PLCB2; PLC-beta-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific)
Pricing
product-image-AAA31358_IHC7.jpg
product-image-AAA31358_IHC7.jpg IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

ENSA, Polyclonal Antibody (Cat# AAA31358)

Full Name
Phospho-ENSA (Ser67/Ser62) Antibody
Gene Names
ENSA; ARPP-19e
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31079_IHC7.jpg
product-image-AAA31079_IHC7.jpg IHC (Immunohistochemistry) (AAA31079 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MCL1, Polyclonal Antibody (Cat# AAA31079)

Full Name
MCL1 Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA23915_APP6.jpg
product-image-AAA23915_APP6.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Estrogen Receptor, alpha, Monoclonal Antibody (Cat# AAA23915)

Full Name
Estrogen Receptor, alpha (Marker of Estrogen Dependence)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
product-image-AAA11663_IHC7.jpg
product-image-AAA11663_IHC7.jpg IHC (Immunohistochemistry) (Figure 7. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (AAA11663).Lipocalin 2 was detected in frozen section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lipocalin 2 Antibody (AAA11663) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Lipocalin 2, Polyclonal Antibody (Cat# AAA11663)

Full Name
Anti-Lipocalin 2 Antibody
Gene Names
Lcn2; Sip24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
product-image-AAA31035_WB13.jpg
product-image-AAA31035_WB13.jpg WB (Western Blot) (Western blot analysis of Akt phosphorylation expression in B2C Cell line lysates, The lane on the right is treated with the antigen-specific peptide.)

Akt, Polyclonal Antibody (Cat# AAA31035)

Full Name
Phospho-Akt (Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA12374_WB3.jpg
product-image-AAA12374_WB3.jpg WB (Western Blot) (Anti-Mesothelin Antibodies - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.)

MSLN / Mesothelin, Monoclonal Antibody (Cat# AAA12374)

Full Name
Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A Purified
Pricing
product-image-AAA12194_APP7.jpg
product-image-AAA12194_APP7.jpg Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12194)

Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
product-image-AAA12198_APP8.jpg
product-image-AAA12198_APP8.jpg Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12198)

Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
product-image-AAA14866_IHC7.jpg
product-image-AAA14866_IHC7.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of HMGB1  in normal human spleen tissue using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

HMGB1, Monoclonal Antibody (Cat# AAA14866)

Full Name
HMGB1 Monoclonal Antibody
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human (Predicted Reactivity: Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
product-image-AAA14863_FCM4.jpg
product-image-AAA14863_FCM4.jpg FCM (Flow Cytometry) (Fig-4: Intracellular flow analysis of TLR7 in Raji cells using 0.5 ug/10^6 cells of TLR7 antibody (Clone: ABM2C27). Green represents isotype control; red represents anti-TLR7 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

TLR7, Monoclonal Antibody (Cat# AAA14863)

Full Name
TLR7 Monoclonal Antibody
Gene Names
TLR7; TLR7-like
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry
Purity
Purified; Protein G Chromatography
Pricing
product-image-AAA24067_IHC2.jpg
product-image-AAA24067_IHC2.jpg IHC (Immunohistochemistry) (Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma using AAA24067, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of AAA24067 for immunohistochemistry.)

CD163, Polyclonal Antibody (Cat# AAA24067)

Full Name
CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163)
Gene Names
CD163; M130; MM130
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by ammonium sulfate precipitation.
Pricing
product-image-AAA30174_IHC7.jpg
product-image-AAA30174_IHC7.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil.)

CCR2, Monoclonal Antibody (Cat# AAA30174)

Full Name
CCR2 Antibody
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
product-image-AAA31540_IHC6.jpg
product-image-AAA31540_IHC6.jpg IHC (Immunohistchemistry) (Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)

beta-Tubulin, Monoclonal Antibody (Cat# AAA31540)

Full Name
Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)
Gene Names
TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
product-image-AAA31218_IHC6.jpg
product-image-AAA31218_IHC6.jpg IHC (Immunohistchemistry-Paraffin) (AAA31218 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Siglec 7, Polyclonal Antibody (Cat# AAA31218)

Full Name
Siglec 7 Antibody
Gene Names
SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31236_IHC10.jpg
product-image-AAA31236_IHC10.jpg IHC (Immunohistochemistry-Paraffin) (AAA31236 at 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

OVOL2, Polyclonal Antibody (Cat# AAA31236)

Full Name
OVOL2 Antibody
Gene Names
OVOL2; CHED; CHED1; CHED2; PPCD1; ZNF339; EUROIMAGE566589
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA11694_WB.jpg
product-image-AAA11694_WB.jpg WB (Western Blot) (Western blot analysis of Lipoprotein lipase expression in Human fetal liver lysate (AAA11694).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LPL)

Lipoprotein lipase, Monoclonal Antibody (Cat# AAA11694)

Full Name
Anti-Lipoprotein lipase Rabbit Monoclonal Antibody
Gene Names
LPL; LIPD; HDLCQ11
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
product-image-AAA31119_IHC3.png
product-image-AAA31119_IHC3.png IHC (Immunohistochemistry) (AAA31119 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)

GPRC5A, Polyclonal Antibody (Cat# AAA31119)

Full Name
GPRC5A Antibody
Gene Names
GPRC5A; RAI3; TIG1; RAIG1; GPCR5A; PEIG-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
product-image-AAA31193_IF8.jpg
product-image-AAA31193_IF8.jpg IF (Immunofluorescence) (AAA31193 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31193 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

CHD4, Polyclonal Antibody (Cat# AAA31193)

Full Name
CHD4 Antibody
Gene Names
CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31215_IF8.jpg
product-image-AAA31215_IF8.jpg IF (Immunofluorescence) (AAA31215 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31215 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

PDE11A, Polyclonal Antibody (Cat# AAA31215)

Full Name
PDE11A Antibody
Gene Names
PDE11A; PPNAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31178_IF8.jpg
product-image-AAA31178_IF8.jpg IF (Immunofluorescence) (AAA31178 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31178) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

CENPB, Polyclonal Antibody (Cat# AAA31178)

Full Name
CENPB Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA27705_IP6.png
product-image-AAA27705_IP6.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27705)

Full Name
VPS35 Antibody, Clone 11H10: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA27685_IP7.png
product-image-AAA27685_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27685)

Full Name
VPS35 Antibody, Clone 8A3: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA31225_IF6.jpg
product-image-AAA31225_IF6.jpg IF (Immunofluorescence) (AAA31225 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31225) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

KCNK9, Polyclonal Antibody (Cat# AAA31225)

Full Name
KCNK9 Antibody
Gene Names
KCNK9; KT3.2; TASK3; K2p9.1; TASK-3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31075_WB2.jpg
product-image-AAA31075_WB2.jpg WB (Western Blot) (Western blot analysis of ZO-1 using various lysatesLanes 1 - 2: Merged signal (red and green). Green - AAA31075 observed at 195 kDa. Red - loading control, , observed at 55 kDa. Blots were developed with Goat Anti- Rabbit IgG(H+L) FITC–conjugated and Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated secondary antibodies)

ZO 1, Polyclonal Antibody (Cat# AAA31075)

Full Name
ZO 1 Antibody
Gene Names
TJP1; ZO-1
Reactivity
Human, Mouse, Rat, Pig, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
product-image-AAA31141_IF3.jpg
product-image-AAA31141_IF3.jpg IF (Immunofluorescence) (AAA31141 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31141 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)

MUC5B, Polyclonal Antibody (Cat# AAA31141)

Full Name
MUC5B Antibody
Gene Names
MUC5B; MG1; MUC5; MUC9; MUC-5B
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
product-image-AAA30922_WB6.jpg
product-image-AAA30922_WB6.jpg WB (Western Blot) (Western blot analysis on mouse liver tissue lysate using E-cadherin Antibody)

E-cadherin, Polyclonal Antibody (Cat# AAA30922)

Full Name
E-cadherin Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA31338_APP14.jpg
product-image-AAA31338_APP14.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MAP2, Polyclonal Antibody (Cat# AAA31338)

Full Name
MAP2 Antibody
Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
product-image-AAA31006_WB19.jpg
product-image-AAA31006_WB19.jpg WB (Western Blot) (Western blot analysis of Phospho-KIT (Tyr703) expression in various lysates)

KIT, Polyclonal Antibody (Cat# AAA31006)

Full Name
Phospho-KIT (Tyr703) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31368_IHC6.jpg
product-image-AAA31368_IHC6.jpg IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IRAK1, Polyclonal Antibody (Cat# AAA31368)

Full Name
Phospho-IRAK1 (Thr209) Antibody
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Dog (82%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA12232_APP6.gif
product-image-AAA12232_APP6.gif Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name
RAT ANTI TUBULIN ALPHA:HRP
Applications
Immunohistochemistry, Western Blot
Pricing
product-image-AAA12009_APP6.gif
product-image-AAA12009_APP6.gif Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name
RAT ANTI TUBULIN ALPHA
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot
Pricing
product-image-AAA12195_APP9.gif
product-image-AAA12195_APP9.gif Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12195)

Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
product-image-AAA12081_APP10.jpg
product-image-AAA12081_APP10.jpg Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)

V5-TAG, Monoclonal Antibody (Cat# AAA12081)

Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
product-image-AAA29642_IHC6.jpg
product-image-AAA29642_IHC6.jpg IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)

Full Name
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Pricing

Elafin, Polyclonal Antibody (Cat# AAA27749)

Full Name
Anti-Elafin Antibody, Rabbit Polyclonal
Gene Names
PI3; ESI; WAP3; SKALP; WFDC14; cementoin
Applications
ELISA
Purity
Protein A affinity chromatography
Pricing
product-image-AAA26964_IHC3.jpg
product-image-AAA26964_IHC3.jpg IHC (Immunohistochemistry) (Immunofluorescent analysis of HepG2 cells using AAA26964 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

CTH, Polyclonal Antibody (Cat# AAA26964)

Full Name
CTH Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
product-image-AAA30976_WB14.jpg
product-image-AAA30976_WB14.jpg WB (Western Blot) (Western blot analysis of p53 phosphorylation expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

p53, Polyclonal Antibody (Cat# AAA30976)

Full Name
Phospho-p53 (Ser392) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31539_WB8.jpg
product-image-AAA31539_WB8.jpg WB (Western Blot) (Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).)

GAPDH, Monoclonal Antibody (Cat# AAA31539)

Full Name
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Yeast, Hamster
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
product-image-AAA31106_IHC2.jpg
product-image-AAA31106_IHC2.jpg IHC (Immunohistochemistry) (AAA31106 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

GPR141, Polyclonal Antibody (Cat# AAA31106)

Full Name
GPR141 Antibody
Gene Names
GPR141; PGR13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA31038_IHC9.jpg
product-image-AAA31038_IHC9.jpg IHC (Immunohistchemistry) (AAA31038 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IRS-1, Polyclonal Antibody (Cat# AAA31038)

Full Name
Phospho-IRS-1 (Ser639) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31245_IHC10.jpg
product-image-AAA31245_IHC10.jpg IHC (Immunohistochemistry-Paraffin) (AAA31245 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Nesprin 1, Polyclonal Antibody (Cat# AAA31245)

Full Name
Nesprin 1 Antibody
Gene Names
SYNE1; 8B; AMCM; CPG2; ARCA1; EDMD4; KASH1; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(100%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing

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