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1506 results for "Conjugated Secondary Antibody" - showing 1050-1100
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PRC1, Polyclonal Antibody (Cat# AAA31450)
Full Name
Phospho-PRC1 (Thr481) Antibody
Gene Names
PRC1; ASE1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Horse (90%)
Predicted Reactivity: Pig (80%), Horse (90%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31369 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31369 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
FOXM1, Polyclonal Antibody (Cat# AAA31369)
Full Name
Phospho-FOXM1 (Ser35) Antibody
Gene Names
FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target), Monoclonal Antibody (Cat# AAA23916)
Full Name
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target)
Gene Names
ICOSLG; B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stainedwith Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 hat 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
TUBA1A, Monoclonal Antibody (Cat# AAA27031)
Full Name
TUBA1A
Gene Names
Tuba1a; Tuba1; Tuba-1
Reactivity
Human, Rabbit, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PGD2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
PGD2 Receptor, Polyclonal Antibody (Cat# AAA27807)
Full Name
Anti-PGD2 Receptor Antibody
Gene Names
PTGDR; DP; AS1; DP1; ASRT1; PTGDR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(All lanes: FOLR4 Antibody (N-term) at 1:500 dilution Lane 1: HepG2 whole cell lysateLane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FOLR4, Polyclonal Antibody (Cat# AAA28763)
Full Name
FOLR4 Antibody (N-term)
Gene Names
IZUMO1R; JUNO; FOLR4; Folbp3
Reactivity
Human
Applications
Western Blot
Pricing
WB (Western Blot)
(All lanes : Anti-TIGIT Antibody at 1:2000 dilutionLane 1: EL4 whole cell lysateLane 2: human fetal thymus lysateLane 3: human spleen lysateLane 4: HUT78 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
TIGIT, Polyclonal Antibody (Cat# AAA28803)
Full Name
TIGIT Antibody
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human, Mouse
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31188 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31188) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
ARF3, Polyclonal Antibody (Cat# AAA31188)
Full Name
ARF3 Antibody
Reactivity
Human, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31204 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31204 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MSH4, Polyclonal Antibody (Cat# AAA31204)
Full Name
MSH4 Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31047 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
STAT5A/B, Polyclonal Antibody (Cat# AAA31047)
Full Name
Phospho-STAT5A/B (Ser725/730) Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Ser396) expression in various lysates)
Tau, Polyclonal Antibody (Cat# AAA31003)
Full Name
Phospho-Tau (Ser396) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)
NANOG, Polyclonal Antibody (Cat# AAA28706)
Full Name
NANOG Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(AAA31372 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31372 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
RUNX2, Polyclonal Antibody (Cat# AAA31372)
Full Name
Phospho-RUNX2 (S340) Antibody
Gene Names
RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA30998 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Tau, Polyclonal Antibody (Cat# AAA30998)
Full Name
Phospho-Tau (Ser356) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12197)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using GAPDH antibody as the primary antibody. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody (AAA10764) at 1:5000/1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure Time: 60s.)
IgG (H+L), Secondary Antibody (Cat# AAA10764)
Full Name
HRP Goat Anti-Mouse IgG (H+L) Antibody
Applications
Western Blot
Purity
Affinity Purification
Pricing
Application Data
(Fig.2: CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2ug of purified EQ-8D11-C1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by CGE (Fig.2).)
Ovine CD34, Monoclonal Antibody (Cat# AAA14097)
Full Name
anti-ovine CD34 monoclonal antibody
Applications
Flow Cytometry, Competitive ELISA
Purity
Protein G purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31273 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
GCHFR, Polyclonal Antibody (Cat# AAA31273)
Full Name
GCHFR Antibody
Gene Names
GCHFR; P35; GFRP; HsT16933
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(88%), Horse(88%), Dog(88%)
Predicted Reactivity: Bovine(88%), Horse(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31113 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
SLC38A2, Polyclonal Antibody (Cat# AAA31113)
Full Name
SLC38A2 Antibody
Gene Names
SLC38A2; ATA2; SAT2; SNAT2; PRO1068
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31191 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
KMT1E/SETDB1, Polyclonal Antibody (Cat# AAA31191)
Full Name
KMT1E/SETDB1 Antibody
Gene Names
SETDB1; ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(86%)
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31183 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
GDF8/Myostatin, Polyclonal Antibody (Cat# AAA31183)
Full Name
GDF8/Myostatin Antibody
Gene Names
MSTN; GDF8; MSLHP
Reactivity
Human, Mouse, Rat, Sheep
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31171 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31171 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
GJA10, Polyclonal Antibody (Cat# AAA31171)
Full Name
Connexin 59/GJA10 Antibody
Gene Names
GJA9; CX58; CX59; GJA10
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(80%)
Predicted Reactivity: Horse(80%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31176 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31176 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
cbx7, Polyclonal Antibody (Cat# AAA31176)
Full Name
cbx7 Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(FOXD1 Antibody (N-term) (AAA28675) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FOXD1, Polyclonal Antibody (Cat# AAA28675)
Full Name
FOXD1 Antibody (N-term)
Gene Names
FOXD1; FKHL8; FREAC4; FREAC-4
Reactivity
Human, Mouse; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(AAA31037 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IRS-1, Polyclonal Antibody (Cat# AAA31037)
Full Name
Phospho-IRS-1 (Ser636) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31173 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31173 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Nav1.8, Polyclonal Antibody (Cat# AAA31173)
Full Name
Nav1.8 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31205 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31205 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CBX1, Polyclonal Antibody (Cat# AAA31205)
Full Name
CBX1/HP1 beta Antibody
Gene Names
CBX1; CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31150 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25 degree C. Samples were thenincubated with primary Ab(AAA31150 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
Cleaved Caspase 3, Polyclonal Antibody (Cat# AAA31150)
Full Name
Cleaved Caspase 3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(PCSK9 Antibody (N-term) (Cat. #AAA28774) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell(top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PCSK9, Polyclonal Antibody (Cat# AAA28774)
Full Name
PCSK9 Antibody (N-term)
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12193)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: oprF antibody at 5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39.3 kDaObserved band size: 44 kDa)
oprF, Polyclonal Antibody (Cat# AAA27020)
Full Name
oprF Antibody
Reactivity
Pseudomonas aeruginosa
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Adenosine A2a Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Adenosine A2a Receptor, Polyclonal Antibody (Cat# AAA27805)
Full Name
Anti-Adenosine A2a Receptor Antibody
Gene Names
ADORA2A; A2aR; RDC8; ADORA2
Reactivity
Human, Mouse, Rat, Dog
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
NRF2, Monoclonal Antibody (Cat# AAA29775)
Full Name
NRF2 (Phospho-S40) Antibody
Gene Names
NFE2L2; NRF2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31239 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WIPI1, Polyclonal Antibody (Cat# AAA31239)
Full Name
WIPI1 Antibody
Gene Names
WIPI1; ATG18; ATG18A; WIPI49
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Rabbit(100%), Dog(88%), Chicken(86%)
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Rabbit(100%), Dog(88%), Chicken(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31257 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ILF1, Polyclonal Antibody (Cat# AAA31257)
Full Name
ILF1 Antibody
Gene Names
FOXK2; ILF; ILF1; ILF-1; nGTBP
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(100%)
Predicted Reactivity: Horse(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31219 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
LYRIC, Polyclonal Antibody (Cat# AAA31219)
Full Name
LYRIC Antibody
Gene Names
MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(88%), Chicken(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(88%), Chicken(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31259 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SLC1A5, Polyclonal Antibody (Cat# AAA31259)
Full Name
SLC1A5 Antibody
Gene Names
SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31234 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
KMT2A/MLL, Polyclonal Antibody (Cat# AAA31234)
Full Name
KMT2A/MLL Antibody
Gene Names
KMT2A; HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Dog(100%), Chicken(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Dog(100%), Chicken(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31135 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PIP, Polyclonal Antibody (Cat# AAA31135)
Full Name
PIP Antibody
Gene Names
PIP; GPIP4; GCDFP15; GCDFP-15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31261 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31261 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Filaggrin, Polyclonal Antibody (Cat# AAA31261)
Full Name
Filaggrin Antibody
Gene Names
FLI1; EWSR2; SIC-1; BDPLT21
Reactivity
Human, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31192 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31192 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rab4, Polyclonal Antibody (Cat# AAA31192)
Full Name
Rab4 Antibody
Gene Names
RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31223 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31223 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SLIRP, Polyclonal Antibody (Cat# AAA31223)
Full Name
SLIRP Antibody
Gene Names
SLIRP; DC50; PD04872; C14orf156
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(83%), Dog(83%)
Predicted Reactivity: Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(83%), Dog(83%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-STAT6 (Tyr641) expression in various lysates)
STAT6, Polyclonal Antibody (Cat# AAA31045)
Full Name
Phospho-STAT6 (Tyr641) Antibody
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31044 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT1, Polyclonal Antibody (Cat# AAA31044)
Full Name
Phospho-STAT1 (Tyr701) Antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31073 at 1/100 staining Carcinoid tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 107°C.)
SIRT3, Polyclonal Antibody (Cat# AAA31073)
Full Name
SIRT3 Antibody
Gene Names
SIRT3; SIR2L3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Peptide ELISA
Purity
Peptide affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
IRF7, Polyclonal Antibody (Cat# AAA31330)
Full Name
Phospho-IRF7 (Ser471/Ser472) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12192)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA27019 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
ETV2, Polyclonal Antibody (Cat# AAA27019)
Full Name
ETV2 Antibody
Gene Names
ETV2; ER71; ETSRP71
Reactivity
Human
Applications
Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MCF-7 cells using AAA26992 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
USP18, Polyclonal Antibody (Cat# AAA26992)
Full Name
USP18 Antibody
Gene Names
USP18; ISG43; UBP43
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing