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1506 results for "Conjugated Secondary Antibody" - showing 1250-1300
IF (Immunofluorescence)
(Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Vasopressin V2 Receptor, Polyclonal Antibody (Cat# AAA27813)
Full Name
Anti-Vasopressin V2 Receptor Antibody
Gene Names
AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(AAA31128 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IL1A, Polyclonal Antibody (Cat# AAA31128)
Full Name
IL1A Antibody
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(IHC image of AAA27004 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
GBA, Polyclonal Antibody (Cat# AAA27004)
Full Name
GBA Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(PYY Antibody (C-term) (AAA28733) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PYY, Polyclonal Antibody (Cat# AAA28733)
Full Name
PYY Antibody (C-term)
Gene Names
PYY; PYY1; PYY-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(IHC image of AAA27005 diluted at 1:500 and staining in paraffin-embedded human ovarian cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
SRRM4, Polyclonal Antibody (Cat# AAA27005)
Full Name
SRRM4 Antibody
Gene Names
SRRM4; nSR100; KIAA1853; MU-MB-2.76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA26927 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
DHRS3, Polyclonal Antibody (Cat# AAA26927)
Full Name
DHRS3 Antibody
Gene Names
DHRS3; SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NRK Antibody (Center) (AAA28689). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NRK, Polyclonal Antibody (Cat# AAA28689)
Full Name
NRK Antibody (Center)
Gene Names
NRK; NESK
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(AAA31133 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IRAK4, Polyclonal Antibody (Cat# AAA31133)
Full Name
Phospho-IRAK4 (Thr345/Ser346) Antibody
Gene Names
IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31172 at 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
CoREST, Polyclonal Antibody (Cat# AAA31172)
Full Name
CoREST Antibody
Gene Names
RCOR1; RCOR; COREST
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(86%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31238 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
C9orf142, Polyclonal Antibody (Cat# AAA31238)
Full Name
C9orf142 Antibody
Gene Names
PAXX; XLS; C9orf142
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%)
Predicted Reactivity: Pig(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31250 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TFAP4, Polyclonal Antibody (Cat# AAA31250)
Full Name
TFAP4 Antibody
Gene Names
TFAP4; AP-4; bHLHc41
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AF1032 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
alpha-SMA, Polyclonal Antibody (Cat# AAA30933)
Full Name
alpha-SMA Antibody
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in UV treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30958)
Full Name
Phospho-Chk2 (Thr383) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of SGK phosphorylation expression in Insulin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
SGK, Polyclonal Antibody (Cat# AAA30942)
Full Name
Phospho-SGK (Ser422) Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SLK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
SLK, Polyclonal Antibody (Cat# AAA27796)
Full Name
Anti-SLK Antibody
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using UBASH3A Ab. The lane on the left was treated with blocking peptide.)
UBASH3A, Polyclonal Antibody (Cat# AAA31111)
Full Name
UBASH3A Antibody
Gene Names
UBASH3A; TULA; CLIP4; STS-2; TULA-1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31184 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Golgin subfamily A member 4, Polyclonal Antibody (Cat# AAA31184)
Full Name
Golgin subfamily A member 4 Antibody
Gene Names
GOLGA4; GCP2; GOLG; p230; CRPF46; MU-RMS-40.18
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31098 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA31098 1:200) and mouse antibeta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
Piezo1, Polyclonal Antibody (Cat# AAA31098)
Full Name
Piezo1 Antibody
Gene Names
PIEZO1; DHS; Mib; LMPH3; FAM38A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(AAA31221 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31221 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
T Plastin, Polyclonal Antibody (Cat# AAA31221)
Full Name
T Plastin Antibody
Gene Names
PLS3; BMND18; T-plastin
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in TSA treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30963)
Full Name
Phospho-EGFR (Ser1026) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31209 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31209 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Ribosomal Protein S29, Polyclonal Antibody (Cat# AAA31209)
Full Name
Ribosomal Protein S29 Antibody
Gene Names
RPS29; S29; uS14; DBA13
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blotAll lanes: CXCR4 antibody at 2ug/ml+Hela cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40,41 kDaObserved band size: 40 kDa)
CXCR4, Polyclonal Antibody (Cat# AAA26937)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA31186 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31186 1:200) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Cytoplasmic dynein 1 intermediate chain 1, Polyclonal Antibody (Cat# AAA31186)
Full Name
Cytoplasmic dynein 1 intermediate chain 1 Antibody
Gene Names
DYNC1I1; DNCI1; DNCIC1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31103 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31103) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
CCL20, Polyclonal Antibody (Cat# AAA31103)
Full Name
CCL20 Antibody
Gene Names
CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human placenta tissue using AAA28695 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
GAA, Polyclonal Antibody (Cat# AAA28695)
Full Name
GAA Antibody (N-term)
Gene Names
GAA; LYAG
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA31036 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IRS-1, Polyclonal Antibody (Cat# AAA31036)
Full Name
Phospho-IRS-1 (Ser312) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31266 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31266 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Importin 7, Polyclonal Antibody (Cat# AAA31266)
Full Name
Importin 7 Antibody
Gene Names
IPO7; Imp7; RANBP7
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31207 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31207) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IL22 Receptor Alpha, Polyclonal Antibody (Cat# AAA31207)
Full Name
IL22 Receptor Alpha Antibody
Gene Names
IL22RA1; IL22R; CRF2-9; IL22R1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA31013 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
GATA1, Polyclonal Antibody (Cat# AAA31013)
Full Name
Phospho-GATA1 (Ser142) Antibody
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31030 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25A, Polyclonal Antibody (Cat# AAA31030)
Full Name
Phospho-CDC25A (Ser178) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from K562 , using Phospho-IRE1 (Ser724) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
IRE1, Polyclonal Antibody (Cat# AAA31151)
Full Name
Phospho-IRE1 (Ser724) Antibody
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31198 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31198 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SNAP23, Polyclonal Antibody (Cat# AAA31198)
Full Name
SNAP23 Antibody
Gene Names
SNAP23; SNAP-23; SNAP23A; SNAP23B; HsT17016
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Thr212) Antibody expression in mouse brain and rat kidney tissues lysates.)
Tau, Polyclonal Antibody (Cat# AAA31001)
Full Name
Phospho-Tau (Thr212) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Synuclein phosphorylation expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)
Synuclein, Polyclonal Antibody (Cat# AAA31040)
Full Name
Phospho-Synuclein (Ser129) Antibody
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of mTOR phosphorylation expression in mouse liver tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
mTOR, Polyclonal Antibody (Cat# AAA31048)
Full Name
Phospho-mTOR (Ser2448) Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat, Fish
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31099 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31099) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue))
NOD2, Polyclonal Antibody (Cat# AAA31099)
Full Name
NOD2 Antibody
Gene Names
NOD2; CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Pricing
IF (Immunofluorescence)
(Figure 2. Indirect immunofluorescence staining of human kidney tissue section with (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.)
vimentin, Monoclonal Recombinant Antibody (Cat# AAA14575)
Full Name
Recombinant rabbit anti vimentin
Reactivity
Human, mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoblot
Purity
The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis.
Pricing
IHC (Immunohistochemistry)
(AAA31016 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CREB, Polyclonal Antibody (Cat# AAA31016)
Full Name
Phospho-CREB (Ser133) Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Src Antibody.)
Src, Polyclonal Antibody (Cat# AAA31086)
Full Name
Src Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31237 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31237 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
DBT, Polyclonal Antibody (Cat# AAA31237)
Full Name
DBT Antibody
Gene Names
DBT; E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2
Reactivity
Human,Mouse,Ra
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31012 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ATF2, Polyclonal Antibody (Cat# AAA31012)
Full Name
Phospho-ATF2 (Thr71/53) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31025 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Cofilin, Polyclonal Antibody (Cat# AAA31025)
Full Name
Phospho-Cofilin (Ser3) Antibody
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ATF6, Polyclonal Antibody (Cat# AAA31376)
Full Name
Phospho-ATF6 (Thr166) Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Horse (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)
WNT5A, Polyclonal Antibody (Cat# AAA28750)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (AAA11653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (AAA11653) at 0.25ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA11653)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(AAA30920 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Akt, Polyclonal Antibody (Cat# AAA30920)
Full Name
Phospho-Akt(Ser473) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
FCM (Flow Cytometry)
(Figure-1: Cell surface flowcytometry analysis of CLEC1on human PBMC (Monocytes) using 0.5 ug/10^6 Cells of Anti-CLEC1 antibody. Green represent isotype control and red represent Anti human CLEC1 antibody. Goat anti mouse PE conjugated was used as the secondary antibody.)
CLEC1, Monoclonal Antibody (Cat# AAA14882)
Full Name
Monoclonal Antibody to CLEC1 (Clone: ABM2H82)
Gene Names
CLEC1A; CLEC1; CLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
Protein G Chromatography Purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31167 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
beta COP, Polyclonal Antibody (Cat# AAA31167)
Full Name
beta COP Antibody
Gene Names
COPB1; COPB
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31114 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31114) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
ARX, Polyclonal Antibody (Cat# AAA31114)
Full Name
ARX Antibody
Gene Names
ARX; ISSX; PRTS; CT121; EIEE1; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Pricing