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1506 results for "Conjugated Secondary Antibody" - showing 850-900
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with AAA14796 at 1:25 dilution, followed by Dylight® 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA14796)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin)
Reactivity
Human
Applications
EL/EIA, WB, IHC, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of CACO-2 cells using anti-Galectin 2/LGALS2 antibody (AAA19287).Overlay histogram showing CACO-2 cells stained with AAA19287 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin 2/LGALS2 Antibody (AAA19287, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Galectin 2/LGALS2, Polyclonal Antibody (Cat# AAA19287)
Full Name
Anti-Galectin 2/LGALS2 Antibody
Gene Names
LGALS2; HL14
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of THP-1 cells using anti-Alpha B Crystallin/CRYAB antibody (AAA19277).Overlay histogram showing THP-1 cells stained with AAA19277 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha B Crystallin/CRYAB Antibody (AAA19277, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alpha B Crystallin/CRYAB, Polyclonal Antibody (Cat# AAA19277)
Full Name
Anti-Alpha B Crystallin/CRYAB Antibody
Gene Names
CRYAB; CRYA2; CTPP2; HSPB5; CMD1II
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation MCF7(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.)
HDAC1, Polyclonal Antibody (Cat# AAA18851)
Full Name
HDAC1 Antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Reactivity
Human
Applications
EIA, WB, IHC, ChIP
Purity
>95%, Protein G Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with RUNX1+RUNX2+RUNX3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
RUNX1+RUNX2+RUNX3, Monoclonal Antibody (Cat# AAA30202)
Full Name
RUNX1+RUNX2+RUNX3 Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Histone H2B, Monoclonal Antibody (Cat# AAA30192)
Full Name
Histone H2B Antibody
Gene Names
HIST1H2BK; H2BK; H2B/S; H2BFT; H2BFAiii
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HL-60 cells using anti-PARP antibody (AAA19346).Overlay histogram showing HL-60 cells stained with AAA19346 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PARP Antibody (AAA19346, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PARP, Monoclonal Antibody (Cat# AAA19346)
Full Name
Anti-PARP Antibody (monoclonal, 10G9)
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 18. Flow Cytometry analysis of A431 cells using anti-LSM7 antibody (AAA19327).Overlay histogram showing A431 cells stained with AAA19327 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM7 Antibody (AAA19327, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LSM7, Polyclonal Antibody (Cat# AAA19327)
Full Name
Anti-LSM7 Antibody
Gene Names
LSM7; YNL147W
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ERGI3, Polyclonal Antibody (Cat# AAA29942)
Full Name
ERGI3 Antibody
Gene Names
ERGIC3; Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
EZH2, Polyclonal Antibody (Cat# AAA28665)
Full Name
EZH2 Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Hip1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
HIP1, Polyclonal Antibody (Cat# AAA29933)
Full Name
HIP1 Antibody
Gene Names
HIP1; HIP-I; ILWEQ
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
Application Data
(Overlay histogram showing Hela cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Nestin, Monoclonal Antibody (Cat# AAA18857)
Full Name
Mouse Anti-Nestin monoclonal Antibody
Gene Names
NES; Nbla00170
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G Purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U937 cells using anti-SEC14L3/TAP2 antibody (AAA19342).Overlay histogram showing U937 cells stained with AAA19342 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC14L3/TAP2 Antibody (AAA19342, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SEC14L3/TAP2, Polyclonal Antibody (Cat# AAA19342)
Full Name
Anti-SEC14L3/TAP2 Antibody
Gene Names
SEC14L3; TAP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U87 cells using anti-PSMB5/MB1 antibody (AAA19274).Overlay histogram showing U87 cells stained with AAA19274 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB5/MB1 Antibody (AAA19274, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PSMB5/MB1, Polyclonal Antibody (Cat# AAA19274)
Full Name
Anti-PSMB5/MB1 Antibody
Gene Names
PSMB5; X; MB1; LMPX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blotAll lanes: APOA1antibody at 2ug/mlLane 1:mouse liver tissueLane 2:mouse lung tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 30kDaObserved band size: 30kDa)
Apolipoprotein A-I, Polyclonal Antibody (Cat# AAA18816)
Full Name
Rabbit anti-Bovine Apolipoprotein A-I polyclonal Antibody
Reactivity
Bovine, Mouse
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A431 cells with Desmoglein 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Desmoglein 2, Monoclonal Antibody (Cat# AAA30463)
Full Name
Desmoglein 2 Antibody
Gene Names
DSG2; HDGC; CDHF5; ARVC10; ARVD10; CMD1BB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
CACNG4, Polyclonal Antibody (Cat# AAA29944)
Full Name
CACNG4 Antibody
Reactivity
Human, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
FCM (Flow Cytometry)
(TYSY Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
TYSY, Polyclonal Antibody (Cat# AAA28647)
Full Name
TYSY Antibody (C-term)
Gene Names
TYMS; TS; TMS; HST422
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Hela cells using anti-PDE4D antibody (AAA19143).Overlay histogram showing Hela cells stained with AAA19143 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4D Antibody (AAA19143,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDE4D, Polyclonal Antibody (Cat# AAA19143)
Full Name
Anti-PDE4D Picoband antibody
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of GCHFR using anti-GCHFR antibody (AAA19322).GCHFR was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GCHFR Antibody (AAA19322) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
GCHFR, Polyclonal Antibody (Cat# AAA19322)
Full Name
Anti-GCHFR Antibody
Gene Names
GCHFR; P35; GFRP; HsT16933
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of eRF1/ETF1 using anti- eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
eRF1/ETF1, Monoclonal Antibody (Cat# AAA19383)
Full Name
Anti-eRF1/ETF1 Antibody (monoclonal, 3E5)
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of LLC cells using anti-Synaptotagmin 1 antibody (AAA19158).Overlay histogram showing LLC cells stained with AAA19158 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (AAA19158,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Synaptotagmin 1, Polyclonal Antibody (Cat# AAA19158)
Full Name
Anti-Synaptotagmin 1 Picoband antibody
Gene Names
SYT1; P65; SYT; SVP65
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of MCF-7 cells using anti-DDX1 antibody (AAA19378).Overlay histogram showing MCF-7 cells stained with AAA19378 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX1 Antibody (AAA19378, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DDX1, Monoclonal Antibody (Cat# AAA19378)
Full Name
Anti-DDX1 Antibody (monoclonal, 3I10)
Gene Names
DDX1; DBP-RB; UKVH5d
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of THP-1 cells using anti-EPRS1/PARS antibody (AAA19268).Overlay histogram showing THP-1 cells stained with AAA19268 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPRS1/PARS Antibody (AAA19268, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EPRS1/PARS, Polyclonal Antibody (Cat# AAA19268)
Full Name
Anti-EPRS1/PARS Antibody
Gene Names
EPRS; EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(TMPRSS2 Antibody (Center)(AAA28646) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
TMPRSS2, Polyclonal Antibody (Cat# AAA28646)
Full Name
TMPRSS2 Antibody (Center)
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Reactivity: Human, Mouse
Predicted: Mouse
Predicted: Mouse
Applications
WB, EIA, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26858)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (Biotin)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U20S cells using anti-SV2A antibody (AAA19279).Overlay histogram showing U20S cells stained with AAA19279 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SV2A Antibody (AAA19279, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SV2A, Polyclonal Antibody (Cat# AAA19279)
Full Name
Anti-SV2A Antibody
Gene Names
SV2A; SV2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HEPG2 cells using anti-ALDH1L1 antibody (AAA19297).Overlay histogram showing HEPG2 cells stained with AAA19297 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1L1 Antibody (AAA19297 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ALDH1L1, Polyclonal Antibody (Cat# AAA19297)
Full Name
Anti-ALDH1L1 Antibody
Gene Names
ALDH1L1; FDH; FTHFD; 10-fTHF; 10-FTHFDH
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-REA/PHB2 antibody (AAA19374).Overlay histogram showing HepG2 cells stained with AAA19374 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- REA/PHB2 Antibody (AAA19374, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
REA/PHB2, Monoclonal Antibody (Cat# AAA19374)
Full Name
Anti-REA/PHB2 Antibody (monoclonal, 2B5)
Gene Names
PHB2; BAP; REA; p22; Bap37; BCAP37; PNAS-141
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of SiHa cells using anti-PRDM14 antibody (AAA19283).Overlay histogram showing SiHa cells stained with AAA19283 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM14 Antibody (AAA19283, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRDM14, Polyclonal Antibody (Cat# AAA19283)
Full Name
Anti-PRDM14 Antibody
Gene Names
PRDM14; PFM11
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IgG (H+L), Secondary Antibody (Cat# AAA26993)
Full Name
Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated
Reactivity
Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from goat antiserum by affinity-chromatography using specific immunogen.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
RYR2, Polyclonal Antibody (Cat# AAA29945)
Full Name
RYR2 Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of Jurkat cells using anti-Ki67 antibody (AAA19350).Overlay histogram showing Jurkat cells stained with AAA19350 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67 Antibody (AAA19350, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Ki67, Monoclonal Antibody (Cat# AAA19350)
Full Name
Anti-Ki67 Antibody (monoclonal, 5E12)
Gene Names
MKI67; KIA
Reactivity
Human
Applications
IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-DR6/TNFRSF21 antibody (AAA19292).Overlay histogram showing A549 cells stained with AAA19292 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DR6/TNFRSF21 Antibody (AAA19292, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DR6/TNFRSF21, Polyclonal Antibody (Cat# AAA19292)
Full Name
Anti-DR6/TNFRSF21 Antibody
Gene Names
TNFRSF21; DR6; CD358; BM-018
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U87 cells using anti-GNG2 antibody (AAA19312).Overlay histogram showing U87 cells stained with AAA19312 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG2 Antibody (AAA19312, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GNG2, Polyclonal Antibody (Cat# AAA19312)
Full Name
Anti-GNG2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of 293T cells using anti-CDC45L antibody (AAA19360).Overlay histogram showing 293T cells stained with AAA19360 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- CDC45L Antibody (AAA19360, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CDC45L, Monoclonal Antibody (Cat# AAA19360)
Full Name
Anti-CDC45L Antibody (monoclonal, 6H6)
Gene Names
CDC45; CDC45L; CDC45L2; PORC-PI-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CISD2 using anti-CISD2 antibody (AAA19311).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey kidney tissue lysatesLane 5: human SW620 whole cell lysatesLane 6: human Raji whole cell lysatesLane 7: rat kidney tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CISD2 antigen affinity purified polyclonal antibody (Catalog # AAA19311) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CISD2 at approximately 15KD. The expected band size for CISD2 is at 15KD.)
CISD2, Polyclonal Antibody (Cat# AAA19311)
Full Name
Anti-CISD2 Antibody
Gene Names
CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of RH35 cells using anti- EIF4A1 antibody (AAA19380).Overlay histogram showing RH35 cells stained with AAA19380 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19380, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EIF4A1, Monoclonal Antibody (Cat# AAA19380)
Full Name
Anti-EIF4A1 Antibody (monoclonal, 11B8)
Gene Names
EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of MCF-7 cells using anti-EHD3 antibody (AAA19295).Overlay histogram showing MCF-7 cells stained with AAA19295 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD3 Antibody (AAA19295, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EHD3, Polyclonal Antibody (Cat# AAA19295)
Full Name
Anti-EHD3 Antibody
Gene Names
EHD2; PAST2
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)
SRC, Monoclonal Antibody (Cat# AAA28639)
Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
METTL1, Polyclonal Antibody (Cat# AAA19336)
Full Name
Anti-METTL1 Antibody
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with HKDC1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
HKDC1, Polyclonal Antibody (Cat# AAA29946)
Full Name
HKDC1 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Protein affinity purified
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: MUC1Sample Type: HepG2Lane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)
MUC1, Polyclonal Antibody (Cat# AAA23492)
Full Name
MUC1 antibody - C-terminal region
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Pig
Applications
IHC, WB
Purity
Affinity Purified
Pricing
DB (Dot Blot)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Phospho-HIST1H3B3 (S10), Polyclonal Antibody (Cat# AAA28670)
Full Name
Phospho-HIST1H3B3 (S10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
DB, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
SHH, Polyclonal Antibody (Cat# AAA23516)
Full Name
SHH antibody - N-terminal region
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (AAA19291).Overlay histogram showing U937 cells stained with AAA19291 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (AAA19291, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)
Full Name
Anti-Coronin 1a/TACO/CORO1A Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with Galectin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Galectin 8, Monoclonal Antibody (Cat# AAA30457)
Full Name
Galectin 8 Antibody
Gene Names
LGALS8; Gal-8; PCTA1; PCTA-1; Po66-CBP
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS, IP
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-TMPRSS3 antibody (AAA19289).Overlay histogram showing A431 cells stained with AAA19289 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMPRSS3 Antibody (AAA19289, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TMPRSS3, Polyclonal Antibody (Cat# AAA19289)
Full Name
Anti-TMPRSS3 Antibody
Gene Names
TMPRSS3; DFNB8; DFNB10; ECHOS1; TADG12
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of 293T cells using anti-TBP-1/PSMC3 antibody (AAA19314).Overlay histogram showing 293T cells stained with AAA19314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBP-1/PSMC3 Antibody (AAA19314, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TBP-1/PSMC3, Polyclonal Antibody (Cat# AAA19314)
Full Name
Anti-TBP-1/PSMC3 Antibody
Gene Names
PSMC3; TBP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U937 cells using anti-BDH1 antibody (AAA19328).Overlay histogram showing U937 cells stained with AAA19328 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDH1 Antibody (AAA19328,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BDH1, Polyclonal Antibody (Cat# AAA19328)
Full Name
Anti-BDH1 Antibody
Gene Names
BDH1; BDH; SDR9C1
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing