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Reactivity
Gene Name
Isotype
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Application
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597 results for "Tag Loading Control Antibodies" - showing 550-597
WB (Western Blot)
(MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.)
MKRN2, Monoclonal Antibody (Cat# AAA24276)
Full Name
MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070) (AP)
Gene Names
MKRN2; RNF62; HSPC070
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
ABL2, Monoclonal Antibody (Cat# AAA25592)
Full Name
ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) (PE)
Gene Names
ABL2; ARG; ABLL
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA24234)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (AP)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IRAK1, Monoclonal Antibody (Cat# AAA24544)
Full Name
IRAK1 (Interleukin-1 Receptor-associated Kinase 1, IRAK-1, IRAK) APC
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human
Applications
EIA, IF, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
PIP5K3, Monoclonal Antibody (Cat# AAA25795)
Full Name
PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981
Gene Names
PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
RAD51C, Monoclonal Antibody (Cat# AAA25517)
Full Name
RAD51C (DNA Repair Protein RAD51 Homolog 3, R51H3, RAD51 Homolog C, RAD51-like Protein 2, MGC104277, RAD51L2) (HRP)
Gene Names
RAD51C; FANCO; R51H3; BROVCA3; RAD51L2
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
CITED1, Monoclonal Antibody (Cat# AAA24761)
Full Name
CITED1 (CBP/p300 Interacting Transactivator 1, Cbp/p300-interacting Transactivator with Glu/Asp-rich Carboxy-terminal Domain 1, Melanocyte-specific Protein1) (Biotin)
Gene Names
CITED1; MSG1
Reactivity
Human, Rat
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CSK monoclonal antibody. Western Blot analysis of CSK expression in Hela NE.)
CSK, Monoclonal Antibody (Cat# AAA24769)
Full Name
CSK (Tyrosine-protein Kinase CSK, C-SRC Kinase, Protein-tyrosine Kinase CYL) (Biotin)
Reactivity
Human
Applications
EIA, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
TAF7, Monoclonal Antibody (Cat# AAA25565)
Full Name
TAF7 (TAF2F, TAFII55, Transcription Initiation Factor TFIID Subunit 7, RNA Polymerase II TBP-associated Factor Subunit F, Transcription Initiation Factor TFIID 55kD Subunit, TAF(II)55, TAFII-55) (HRP)
Gene Names
TAF7; TAF2F; TAFII55
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
CRKL, Monoclonal Antibody (Cat# AAA25064)
Full Name
CRKL (Crk-like Protein) (FITC)
Reactivity
Human
Applications
EIA, IF, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
CRKL, Monoclonal Antibody (Cat# AAA24176)
Full Name
CRKL (Crk-like Protein) (AP)
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (37kD).)
RBM9, Monoclonal Antibody (Cat# AAA25815)
Full Name
RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA) (PE)
Gene Names
RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3
Reactivity
Human, Mouse
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
PPM1B, Monoclonal Antibody (Cat# AAA24326)
Full Name
PPM1B (Protein Phosphatase 1B, PP2C-beta, Protein Phosphatase 2C Isoform beta, PP2CB, MGC21657) (AP)
Gene Names
PPM1B; PP2CB; PP2CBETA; PP2C-beta; PPC2BETAX; PP2C-beta-X
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Survivin, Monoclonal Antibody (Cat# AAA24380)
Full Name
Survivin (Apoptosis Inhibitor 4, API4, Apoptosis Inhibitor Survivin, Baculoviral IAP Repeat Containing Protein 5, BIRC5, EPR-1, IAP4) (AP)
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ADPGK, Monoclonal Antibody (Cat# AAA24419)
Full Name
ADPGK (ADP-dependent Glucokinase, ADP-GK, RbBP-35, PSEC0260, 2610017G09Rik, DKFZp434B195) APC
Gene Names
ADPGK; ADP-GK; 2610017G09Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
TCF19, Monoclonal Antibody (Cat# AAA24385)
Full Name
TCF19 (Transcription Factor 19, TCF-19, SC1, SC1-1, Transcription Factor SC1) (AP)
Gene Names
TCF19; SC1; TCF-19
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ABL2, Monoclonal Antibody (Cat# AAA24411)
Full Name
ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) APC
Gene Names
ABL2; ARG; ABLL
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CBS, Monoclonal Antibody (Cat# AAA24452)
Full Name
CBS (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) APC
Gene Names
CBS; HIP4
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CAPNS1, Monoclonal Antibody (Cat# AAA24156)
Full Name
CAPNS1 (Calpain Small Subunit 1, CSS1, Calcium-activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calcium-dependent Protease Small Subunit, CDPS, Calcium-dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CAPN4, CAPNS) (AP)
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CAMKIV, Monoclonal Antibody (Cat# AAA24153)
Full Name
CAMKIV (Calcium/Calmodulin-dependent Protein Kinase Type IV, CAM Kinase-GR, CaMK IV, CAMK4) (AP)
Gene Names
CAMK4; caMK; CaMKIV; CaMK IV; CaMK-GR
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ARNT, Monoclonal Antibody (Cat# AAA24140)
Full Name
ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT Protein, Class E Basic Helix-loop-helix Protein 2, bHLHe2, Dioxin Receptor, Nuclear Translocator, Hypoxia-inducible Factor 1-beta, HIF-1-beta, HIF1-beta, BHLHE2) (AP)
Gene Names
ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
RAD18, Monoclonal Antibody (Cat# AAA24334)
Full Name
RAD18 (E3 Ubiquitin-protein Ligase RAD18, Postreplication Repair Protein RAD18, hRAD18, hHR18, RING Finger Protein 73, RNF73) (AP)
Gene Names
RAD18; RNF73
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ABCF2, Monoclonal Antibody (Cat# AAA24409)
Full Name
ABCF2 (ATP-binding Cassette Sub-family F Member 2, Iron-inhibited ABC Transporter 2, HUSSY-18, DKFZp586K1823, EST133090, M-ABC1) APC
Gene Names
ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
TGIF2, Monoclonal Antibody (Cat# AAA24386)
Full Name
TGIF2 (TGF-beta-induced Transcription Factor 2, TGFB-induced Factor 2, Homeobox Protein TGIF2, 5'-TG-3'-interacting Factor 2) (AP)
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
UGP2, Monoclonal Antibody (Cat# AAA24401)
Full Name
UGP2 (UTP--glucose-1-phosphate Uridylyltransferase, UDP-glucose Pyrophosphorylase, UDPGP, UGPase, UGP1) (AP)
Gene Names
UGP2; UDPG; UGP1; UDPGP; UGPP1; UGPP2; UDPGP2; pHC379
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CDC25C, Monoclonal Antibody (Cat# AAA24164)
Full Name
CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (AP)
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
RSK3, Monoclonal Antibody (Cat# AAA24347)
Full Name
RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-
Gene Names
RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ENO3, Monoclonal Antibody (Cat# AAA24202)
Full Name
ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase) (AP)
Gene Names
ENO3; MSE; GSD13
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
WB (Western Blot)
(STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)
STK33, Monoclonal Antibody (Cat# AAA25856)
Full Name
STK33 (Serine/Threonine Kinase 33) (PE)
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
DUSP6, Monoclonal Antibody (Cat# AAA24194)
Full Name
DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (AP)
Gene Names
DUSP6; HH19; MKP3; PYST1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
MUTYH, Monoclonal Antibody (Cat# AAA24577)
Full Name
MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) APC
Gene Names
MUTYH; MYH
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(ADPGK monoclonal antibody. Western Blot analysis of ADPGK expression in Raw 264.7.)
ADPGK, Monoclonal Antibody (Cat# AAA25600)
Full Name
ADPGK (ADP-dependent Glucokinase, ADP-GK, RbBP-35, PSEC0260, 2610017G09Rik, DKFZp434B195) (PE)
Gene Names
ADPGK; ADP-GK; 2610017G09Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA25345)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (HRP)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
PKNOX2, Monoclonal Antibody (Cat# AAA24320)
Full Name
PKNOX2 (PREP2, Homeobox Protein PKNOX2, Homeobox Protein PREP-2, PBX/Knotted Homeobox 2, FLJ13074) (AP)
Gene Names
PKNOX2; PREP2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
PITX1, Monoclonal Antibody (Cat# AAA24319)
Full Name
PITX1 (Pituitary Homeobox 1, Paired-like Homeodomain Transcription Factor 1, Homeobox Protein PITX1, Hindlimb-expressed Homeobox Protein Backfoot, PTX1, BFT) (AP)
Gene Names
PITX1; BFT; CCF; POTX; PTX1; LBNBG
Reactivity
Human
Applications
EIA, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
DUSP6, Monoclonal Antibody (Cat# AAA24490)
Full Name
DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) APC
Gene Names
DUSP6; HH19; MKP3; PYST1
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CDK2, Monoclonal Antibody (Cat# AAA24460)
Full Name
CDK2 (Cell Division Protein Kinase 2, p33 Protein Kinase) APC
Gene Names
CDK2; CDKN2; p33(CDK2)
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
MRPL12, Monoclonal Antibody (Cat# AAA24279)
Full Name
MRPL12 (Mitochondrial Ribosomal Protein L12, 39S Ribosomal Protein L12, Mitochondrial, 5c5-2, FLJ60124, L12mt, MGC8610, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML12) (AP)
Gene Names
MRPL12; 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IRAK1, Monoclonal Antibody (Cat# AAA24248)
Full Name
IRAK1 (Interleukin-1 Receptor-associated Kinase 1, IRAK-1, IRAK) (AP)
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human
Applications
EIA, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CRKL, Monoclonal Antibody (Cat# AAA24472)
Full Name
CRKL (Crk-like Protein) APC
Reactivity
Human
Applications
EIA, IF, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CDC25C, Monoclonal Antibody (Cat# AAA24459)
Full Name
CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) APC
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
EIA, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
LCK, Monoclonal Antibody (Cat# AAA24254)
Full Name
LCK (Tyrosine-protein Kinase Lck, Leukocyte C-terminal Src Kinase, LSK, Lymphocyte Cell-specific Protein-tyrosine Kinase, Protein YT16, Proto-oncogene Lck, T Cell-specific Protein-tyrosine Kinase, p56-LCK) (AP)
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Reactivity
Human
Applications
EIA, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing