SDS-PAGE
RNA-splicing ligase RtcB Recombinant Protein | rtcB recombinant protein
Recombinant Escherichia coli RNA-splicing ligase RtcB
Gene Names
rtcB; ECK3407; JW3384; yhgL
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
RNA-splicing ligase RtcB; N/A; Recombinant Escherichia coli RNA-splicing ligase RtcB; rtcB recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-408aa; Full Length
Sequence
MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG
Sequence Length
408
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for rtcB recombinant protein
RNA ligase that mediates the joining of broken tRNA-like st-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like st-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester, a step that requires GTP. Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-PO4 (or cyclic-PO4) and 5'-OH ends that cannot be sealed by classical DNA ligases.
Product Categories/Family for rtcB recombinant protein
References
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon.Genschik P., Drabikowski K., Filipowicz W.J. Biol. Chem. 273:25516-25526(1998) RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.Tanaka N., Shuman S.J. Biol. Chem. 286:7727-7731(2011) RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.Tanaka N., Meineke B., Shuman S.J. Biol. Chem. 286:30253-30257(2011) Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-Phosphate/5'-hydroxyl ligation reactions.Tanaka N., Chakravarty A.K., Maughan B., Shuman S.J. Biol. Chem. 286:43134-43143(2011) The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent.Chakravarty A.K., Shuman S.Nucleic Acids Res. 40:8558-8567(2012) RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl) -GMP and polynucleotide-(3') pp(5') G intermediates.Chakravarty A.K., Subbotin R., Chait B.T., Shuman S.Proc. Natl. Acad. Sci. U.S.A. 109:6072-6077(2012) Rewriting the rules for end joining via enzymatic splicing of DNA 3'-PO4 and 5'-OH ends.Das U., Chakravarty A.K., Remus B.S., Shuman S.Proc. Natl. Acad. Sci. U.S.A. 110:20437-20442(2013)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47.2 kDa
NCBI Official Full Name
RNA-splicing ligase
NCBI Official Symbol
rtcB
NCBI Official Synonym Symbols
ECK3407; JW3384; yhgL
NCBI Protein Information
RNA-splicing ligase
UniProt Protein Name
RNA-splicing ligase RtcB
UniProt Gene Name
rtcB
UniProt Synonym Gene Names
yhgL
UniProt Entry Name
RTCB_ECOLI
Similar Products
Product Notes
The rtcB rtcb (Catalog #AAA115516) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-408aa; Full Length. The amino acid sequence is listed below: MNYELLTTEN APVKMWTKGV PVEADARQQL INTAKMPFIF KHIAVMPDVH LGKGSTIGSV IPTKGAIIPA AVGVDIGCGM NALRTALTAE DLPENLAELR QAIETAVPHG RTTGRCKRDK GAWENPPVNV DAKWAELEAG YQWLTQKYPR FLNTNNYKHL GTLGTGNHFI EICLDESDQV WIMLHSGSRG IGNAIGTYFI DLAQKEMQET LETLPSRDLA YFMEGTEYFD DYLKAVAWAQ LFASLNRDAM MENVVTALQS ITQKTVRQPQ TLAMEEINCH HNYVQKEQHF GEEIYVTRKG AVSARAGQYG IIPGSMGAKS FIVRGLGNEE SFCSCSHGAG RVMSRTKAKK LFSVEDQIRA TAHVECRKDA EVIDEIPMAY KDIDAVMAAQ SDLVEVIYTL RQVVCVKG. It is sometimes possible for the material contained within the vial of "RNA-splicing ligase RtcB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.