At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes H3K14ac (EPL) - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated (Lane 2) were detected with anti-H3K14ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated can be detected by anti-H3K14ac antibody.)
Application Data (Recombinant Mononucleosomes H3K14ac (EPL) - biotin, DNA gel. Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K14ac. Intact mononucleosomes H3K14ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3K14ac (EPL) - biotin. Recombinant Mononucleosomes H3K14ac (EPL) were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 13% SDS-PAGE gel and stained with Commassie blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes H3K14ac are pulled down by streptavidin beads. * indicates streptavidin.)
WB (Western Blot) (Recombinant PRMT4 (CARM1) protein activity assay 0.5 ug Histone H3.1 were incubated with 0, 0.05 ug, 0.1 ug, 0.2 ug PRMT4 (CARM1) in 30 ul reaction system for 2 hours at room temperature, respectively. 6 ul reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody). The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).)
SDS-PAGE (Recombinant PRMT4 (CARM1) protein gel 8% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: >88% )
Application Data (HTRF assay for SRC protein activity 1 uM TK substrate was incubated with different concentrations of SRC protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for SRC protein activity 1 uM TK substrate was incubated with different concentrations of SRC protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant SRC protein gel 10% SDS-PAGE Coomassie staining MW: 61.1 kDa Purity: >95%)
Application Data (HTRF assay for MAPK3 (ERK1) protein activity 1 uM STK S1 substrate was incubated with different concentrations MAPK3 (ERK1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hr. The 10 ul detection reagents containing STK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant MAPK3 (ERK1) protein gel 10% SDS-PAGE Coomassie staining MW: 44.4 kDa Purity: >95%)
SDS-PAGE (Recombinant Mononucleosomes H3.3 (R8C) 12.5% SDS-PAGE gel with Coomassie Blue staining MW: 108 kDa Purity: >95%)
Application Data (Recombinant Mononucleosomes H3.3 (R8C) DNA gel Recombinant Mononucleosomes H3.3 (R8C) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (R8C). Intact mononucleosomes H3.3 (R8C) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3.3 (K18M) 12.5% SDS-PAGE gel stained with Coomassie Blue MW: 108 kDa Purity: >95%)
Application Data (Recombinant Mononucleosomes H3.3 (K18M) DNA gel Recombinant Mononucleosomes H3.3 (K18M) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosomes. Lane 3: Intact mononucleosomes H3.3 (K18M). Intact mononucleosomes H3.3 (K18M) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
Application Data (HTRF assay for EGFR (672-1210) protein activity 1 uM TK substrate was incubated with different concentrations of EGFR (672-1210) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hr. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All reactions were performed at room temp. HTRF assay was used for detection.)
SDS-PAGE (Recombinant EGFR (672-1210) protein gel 10% SDS-PAGE gel with Coomassie Blue staining MW: 63.1 kDa Purity: >55%)
Application Data (HTRF for IGF2BP1 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP1 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF for IGF2BP1 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP1 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant IGF2BP1 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 64.8 kDa Purity: >78%)
Application Data (HTRF assay for RPS6KA3 / MAPKAPK1B activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA3 / MAPKAPK1B protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant RPS6KA3 / MAPKAPK1B protein 10% SDS-PAGE with Coomassie blue staining MW: 84.7 kDa Purity: >95%)
Application Data (HTRF assay for KIT (546-976) activity 1 uM TK substrate was incubated with different concentrations of KIT (546-976) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for KIT (546-976) activity 1 uM TK substrate was incubated with different concentrations of KIT (546-976) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant KIT (546-976) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 50 kDa Purity: >95%)
Application Data (HTRF assay for STK10 / LOK (1-348) activity 1 uM STK S3 substrate was incubated with different concentrations of STK10 / LOK (1-348) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant STK10 / LOK (1-348) protein 10% SDS-PAGE with Coomassie staining MW: 40.4 kDa Purity: >80%)
Application Data (HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant NTRK3 (454-825) protein 10% SDS-PAGE with Coomassie staining MW: 43.5 kDa Purity: >90%)
Application Data (HTRF assay for PDGFRA (550-1089, D842V) activity 1uM TK substrate was incubated with different concentrations of PDGFRA (550-1089, D842V) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl, 1 mM DTT, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant PDGFRA protein gel. 10% SDS-PAGE gel stained with Coomassie Blue. MW: 62.85 Purity: >85%)
Application Data (AMP-Glo assay for UBE2E3 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2E3 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2E3 protein gel. UBE2E3 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 25.08kDa Purity: >90%)
ELISA (ELISA for XRCC1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of XRCC1 were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
SDS-PAGE (Recombinant XRCC1 protein gel. XRCC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 72.01kDa Purity: >80%)
Application Data (HTRF assay for CAMK2D activity 1uM STK S1 substrate was incubated with different concentrations of CAMK2D protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 500uM CaCl2,1uM Calmodulin, 1 mM DTT, 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for ERBB2 (676-1255) activity 1uM TK substrate was incubated with different concentrations of ERBB2 (676-1255) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for Recombinant MECP2 (80-166) protein activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MECP2 (80-166) protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant MECP2 (80-166) protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 12.19kDa Purity: >95%)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.