At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Application Data (HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SDS-PAGE (Recombinant BPTF / FALZ (2791-2911) protein gel. BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 20.3 kDa Purity: > 90%)
Application Data (HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant JMJD2A / KDM4A protein gel. JMJD2A / KDM4A protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 125.3 kDa Purity: > 85%)
Application Data (Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~165 turnovers/ enzyme molecule)
Application Data (Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 (1-21aa) peptide was incubated with Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hour at room temperature. Recombinant KMT2C (MLL3) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
SDS-PAGE (Recombinant KMT2C (MLL3) complex protein gel. Recombinant KMT2C (MLL3) complex run on an SDS-PAGE gel and stained with Coomassie Blue.)
Application Data (HTRF Assay for Recombinant BRD7 (129-236), GST-Tag protein activity. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD7 (129-236) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.)
Application Data (HTRF Assay for Recombinant BRD7 (129-236), GST-Tag protein activity. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD7 (129-236) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.)
SDS-PAGE (Recombinant BRD7 (129-236), GST-Tag protein gel. BRD7 (129-236) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 39 kDa Purity: > 90%)
Application Data (Recombinant JMJD2B / KDM4B protein HTRF activity assay. 3 ?M H3K9me3 peptide was incubated with JMJD2B / KDM4B in reaction buffer for 1 hours at room temperature. JMJD2B / KDM4B enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me2) was measured using H3K9me2-specific antibody.)
SDS-PAGE (Recombinant JMJD2B / KDM4B protein gel. JMJD2B / KDM4B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 126.9 kDa Purity: >70%)
Application Data (MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)
SDS-PAGE (Recombinant MAOA protein gel. MAOA protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (Recombinant HDAC1 activity by HTRF Assay. 1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (Recombinant HDAC1 activity by HTRF Assay. 1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant HDAC1 protein gel. HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 56 kDa Purity> >65%)
Application Data (DUB-Detector assay for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.)
Application Data (DUB-Detector assay for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.)
SDS-PAGE (Recombinant USP7 protein gel 8% SDS-PAGE gel stained with Coomassie Blue MW: 131.3 kDa Purity: >80%)
Application Data (HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
Application Data (HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
SDS-PAGE (Recombinant HDAC3 / NCOR2 complex gel. HDAC3 / NCOR2 complex was run on an 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW of HDAC3: 50.8 kDa MW of NCOR2: 14.6 kDa)
Application Data (HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data (HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
SDS-PAGE (Recombinant HDAC5 protein gel. HDAC5 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes (TH2B) - biotinylated 2 ug Recombinant Mononucleosomes (TH2B) were incubated with DOT1L in reaction buffer for 3 h at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me2). DOT1L only and mononucleosomes only were used as negative control. The Western Blot results show that mononucleosomes are a more suitable substrate for DOT1L than histone octamers. Nucleosomes = Mononucleosomes.)
Application Data (Recombinant Mononucleosomes (TH2B) - biotinylated DNA gel Biotinylated Mononucleosomes (TH2B) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: 601 DNA which was used for assembly of nucleosomes. Lane 2: MW. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form mononucleosomes.)
SDS-PAGE (Streptavidin Pull-down for Recombinant Mononucleosomes (TH2B) - biotinylated Mononucleosomes (TH2B) were pulled down by streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes pulled down by streptavidin (Lane 2) were run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.The SDS-PAGE gel result shows that more than 80% biotinylated nucleosomes (TH2B) are pulled down by streptavidin beads. * indicates streptavidin.)
WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes H3K4me1 (EPL) - biotinylated. Unmodified mononucleosomes (Lane 1) and Mononucleosomes H3K4me1 (Lane 2) were detected with anti-H3K4me1 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Mononucleosomes H3K4me1 can be detected by anti- H3K4me1 antibody. Nucleosomes = Mononucleosomes)
Application Data (Recombinant Mononucleosomes H3K4me1 (EPL) - biotinylated, DNA gel. Mononucleosomes H3K4me1 (EPL) - biotinylated, were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form mononucleosomes. Nucleosomes = Mononucleosomes)
SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3K4me1 (EPL) - biotinylated. Mononucleosomes H3K4me1 (EPL) - biotinylated, were pulled down by streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes (Lane 2) pulled down by streptavidin were run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. The SDS-PAGE gel result shows that almost all of biotinylated polynucleosomes H3K4me1 are pulled down by streptavidin beads. * indicates streptavidin. Nucleosomes = Mononucleosomes)
WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes H3K14ac (EPL) - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated (Lane 2) were detected with anti-H3K14ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated can be detected by anti-H3K14ac antibody.)
Application Data (Recombinant Mononucleosomes H3K14ac (EPL) - biotin, DNA gel. Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K14ac. Intact mononucleosomes H3K14ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3K14ac (EPL) - biotin. Recombinant Mononucleosomes H3K14ac (EPL) were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 13% SDS-PAGE gel and stained with Commassie blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes H3K14ac are pulled down by streptavidin beads. * indicates streptavidin.)
WB (Western Blot) (Recombinant PRMT4 (CARM1) protein activity assay 0.5 ug Histone H3.1 were incubated with 0, 0.05 ug, 0.1 ug, 0.2 ug PRMT4 (CARM1) in 30 ul reaction system for 2 hours at room temperature, respectively. 6 ul reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody). The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).)
SDS-PAGE (Recombinant PRMT4 (CARM1) protein gel 8% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: >88% )
Application Data (HTRF assay for SRC protein activity 1 uM TK substrate was incubated with different concentrations of SRC protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for SRC protein activity 1 uM TK substrate was incubated with different concentrations of SRC protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant SRC protein gel 10% SDS-PAGE Coomassie staining MW: 61.1 kDa Purity: >95%)
Application Data (HTRF assay for MAPK3 (ERK1) protein activity 1 uM STK S1 substrate was incubated with different concentrations MAPK3 (ERK1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hr. The 10 ul detection reagents containing STK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant MAPK3 (ERK1) protein gel 10% SDS-PAGE Coomassie staining MW: 44.4 kDa Purity: >95%)
Application Data (Recombinant PRC2 EZH2 (Y641C) complex activity assay. 1 uM H3K27me2 peptide was incubated with different concentration of PRC2 EZH2 (Y641C) complex in reaction buffer for 3 hour at room temperature. PRC2 EZH2(Y641C) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me3) was measured using H3K27me3-specific antibody.)
Application Data (Recombinant PRC2 EZH2 (Y641C) complex activity assay. 1 uM H3K27me2 peptide was incubated with different concentration of PRC2 EZH2 (Y641C) complex in reaction buffer for 3 hour at room temperature. PRC2 EZH2 (Y641C) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me3) was measured using H3K27me3-specific antibody.)
SDS-PAGE (Recombinant PRC2 EZH2 (Y641C) complex gel. PRC2 EZH2(Y641C) complex was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.