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Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (HTRF assay for SUV39H1 (82-412) activity 3 uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Application Data (HTRF assay for SUV39H1 (82-412) activity 3 uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Application Data (Recombinant PTPN1 protein activity assay 1 mM pNPP was incubated with different concentrations of PTPN1 protein in 100 ul reaction system including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.)
SDS-PAGE (Recombinant PTPN1 protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 76.3 kDa Purity: > 45% )
WB (Western Blot) (Western Blot analysis for Recombinant Mono-nucleosomes H3K4me2 (EPL) – biotin Recombinant Mononucleosomes H3K4me2 (EPL) - biotin (Lane 1) and unmodified nucleosomes (Lane 2) were detected with anti-H3K4me2 antibody and anti-H4 antibody , respectively. H3 was detected as loading control. Only Recombinant Mononucleosomes H3K4me2 (EPL) - biotin can be detected by anti-H3K4me2 antibody.)
Application Data (Recombinant Mononucleosomes H3K4me2 (EPL) - biotin DNA gel Mononucleosomes H3K4me2 (EPL) -biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact nucleosome. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3K4me2 (EPL) - biotinylated. Recombinant Mononucleosomes H3K4me2 (EPL) - biotin were pulled down by streptavidin beads. Input nucleosomes (Lane 1) and the nucleosomes (Lane 2) pulled down by streptavidin were run on a 12.5% SDS-PAGE gel and stained with Commassie bule. The SDS-PAGE gel result shows that almost all of biotinylated nucleosomes H3K4me2 are pulled down by streptavidin beads. The purity of mononucleosomes H3K4me2 (EPL) - biotin is ? 95%.)
Application Data (HTRF assay for MLLT1 / ENL (1-148) activity 3 ?M H3K27cr (crotonylated K27) peptide was incubated with different concentrations of MLLT1 / ENL (1-148) protein in a 10 ?l reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hr, then 10 ?l DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in Reaction Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at RT. HTRF was used for detection.)
Application Data (HTRF assay for MLLT1 / ENL (1-148) activity 3 ?M H3K27cr (crotonylated K27) peptide was incubated with different concentrations of MLLT1 / ENL (1-148) protein in a 10 ?l reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hr, then 10 ?l DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in Reaction Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at RT. HTRF was used for detection.)
SDS-PAGE (Recombinant MLLT1 / ENL (1-148) protein gel 12.5% SDS-PAGE Coomassie staining MW: 21.9 kDa Purity: >85% )
Application Data (HTRF assay for YY1 activity 1 uM oligo dsDNA was incubated with different concentrations of YY1 protein in for 1 hr, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system and incubated for 30 min. HTRF assay was used for detection. )
SDS-PAGE (Recombinant YY1 protein gel 7.5% SDS-PAGE Coomassie staining MW: 46 kDa Purity: >85% )
Application Data (HTRF assay for recombinant AKT1 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT1 protein a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for recombinant AKT1 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT1 protein a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant AKT1 protein gel 10% SDS-PAGE Coomassie staining MW: 60.5 kDa Purity: ?95%)
Application Data (HTRF assay for BRD4 (44-168), GST-Tag activity 1 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (44-168), GST-tag protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRD4 (44-168), GST-Tag activity 1 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (44-168), GST-tag protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD4 (44-168), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 41.2 kDa Purity: >90%)
Application Data (HTRF assay for BRD4 (44-168) activity 3 ?M histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (44-168) protein in a 10 ?l reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ?l DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRD4 (44-168) protein gel. BRD4 (44-168) protein was run on an 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 20 kDa Purity: > 88%)
Application Data (Recombinant EHMT2 (G9a) HTRF activity assay 1 ?M histone H3 (1-21aa.) peptide was incubated with different concentrations of Recombinant EHMT2 (G9A) protein in a 10 ?l reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours, then 10 ?l anti-H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000)
Application Data (Recombinant EHMT2 (G9a) HTRF activity assay 1 ?M histone H3 (1-21aa.) peptide was incubated with different concentrations of Recombinant EHMT2 (G9A) protein in a 10 ?l reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours, then 10 ?l anti-H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
SDS-PAGE (Recombinant EHMT2 (G9a) protein gel. EHMT2 (G9a) protein was run on an 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 134 kDa Purity: > 90%)
SDS-PAGE (Recombinant KMT2A (MLL1)-SET binding assay. KMT2A (MLL1)-SET was incubated with 4 equimolar components of MLL1 complex (ASH1L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE (Recombinant KMT2A (MLL1)-SET protein gel. KMT2A (MLL1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE (Recombinant KMT2D (MLL2)-SET protein binding assay. KMT2D (MLL2)-SET was incubated with equimolar other 4 components of MLL2 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours 4 degree C.All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE (Recombinant KMT2D (MLL2)-SET protein gel. KMT2D (MLL2)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE (Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET was incubated 4 equimolar components of the MLL4 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE (Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
Application Data (Recombinant KMT2A (MLL1) complex activity assay. 3.3 ?M H3K4me0 (1-21aa) peptide was incubated with MLL1 complex in reaction buffer for 3 hour at room temperature. MLL1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Application Data (Recombinant KMT2A (MLL1) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM MLL1 complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control.)
SDS-PAGE (Recombinant KMT2A (MLL1) complex gel. Recombinant MLL1 complex was run on an 12% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (HTRF Assay for Recombinant SMARCA2 / BRM (1367-1511) activity. 3.3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with SMARCCA2/BRM (1367-1511) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.Anti-DYKDDDDK antibody was used to detect reaction products.)
SDS-PAGE (Recombinant SMARCA2 / BRM (1367-1511) protein gel. SMARCA2 / BRM (1367-1511) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
Application Data (Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.)
Application Data (MALDI-TOF Assay for JMJD2D / KDM4D activity . 3.3 uM H3K9me3 peptide was incubated with 100 nM JMJD2D / KDM4D in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K9me3 peptide was used as a negative control.Catalytic Ability: >66 turnovers / enzyme molecule.)
SDS-PAGE (Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
WB (Western Blot) (Recombinant Mononucleosomes (H3.3) - biotinylated activity. 2 ?g Mononucleosomes were incubated with 0 ?g, 0.25 ?g, 0.5 ?g, 1 ?g NSD2-SET in reaction buffer for 3 h at room temperature, respectively. Western blot was used for detecting the generation of reaction products (H3K36me2). Recombinant Histone Octamers was used as a control substrate. The Western blot result shows that mononucleosomes are a more suitable substrate for NSD2-SET than histone octamers.)
Application Data (Recombinant Mononucleosomes (H3.3) - biotinylated DNA gel. Mononucleosomes were run on an agarose gel and stained with ethidium bromide. Lane 1: 601 DNA which was used for assembly of mononucleosomes. Lane 2: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free DNA. The agarose gel result showed almost all of the 601 DNA wrapped histone octamers to form mononucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes (H3.3) - biotinylated protein gel. 40 ug mononucleosomes were incubated with 15 ul streptavidin beads for 1 h at 4C. Samples were centrifuged at 4C, and supernatant was labeled as free histone octamers. Samples were run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that more than 80% biotinylated mononucleosomes were pulled down by streptavidin beads. * indicates streptavidin.)
Application Data (Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Application Data (Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
SDS-PAGE (Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel. SMARCA2 / BRM (1367-1511), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 44.6 kDa Purity: > 85%)
Application Data (3.3 uM H3K36me2 peptide was incubated with 100 nM FBXL11 / KDM2A in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K36me2 peptide was used as negative control.)
SDS-PAGE (Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 128 kDa Purity: > 85%)
Application Data (Recombinant BRD9 (130-259) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD9 (130-259), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products)
SDS-PAGE (Recombinant BRD9 (130-259) SDS-PAGE gel. BRD9 (130-259) protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)
SDS-PAGE (Recombinant MAOB protein gel. MAOB protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (Recombinant HDAC2 activity assay. 1 uM H3K9ac peptide was incubated with different concentrations of HDAC2 protein in a reaction system for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added for 1 h at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant HDAC2 protein gel. HDAC2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 60 kDa Purity: > 45%)
Application Data (HTRF assay for JMJD3 / KDM6B (1043-1682) activity.1 uM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody.)
Application Data (HTRF assay for JMJD3 / KDM6B (1043-1682) activity.1 uM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody. )
SDS-PAGE (Recombinant JMJD3 / KDM6B (1043-1682) SDS-PAGE gel. Recombinant JMJD3 was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 73.4 kDa Purity: > 90%)
WB (Western Blot) (Western Blot analysis for Mononucleosomes (H2A.Bbd) - biotinylated 2 ug Recombinant Mononucleosomes (H2A.Bbd) were incubated with DOT1L in reaction buffer for 3 h at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and mononnucleosomes only were used as negative control.The Western Blot result shows that mononucleosomes are more suitable substrate for DOT1L than histone octamers.)
Application Data (Recombinant Mononucleosomes (H2A.Bbd) - biotinylated DNA gel Biotinylated Mononucleosomes (H2A.Bbd) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: Intact mononucleosomes. Lane 2: 601 DNA which was used for assembly of mononucleosomes. Intact mononucleosomes migrate much higher than free DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form mononucleosomes.)
SDS-PAGE (Streptavidin Pull-down for Recombinant Mononucleosomes (H2A.Bbd) - biotinylated Mononucleosomes (H2A.Bbd) were pulled down by streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes pulled down by streptavidin (Lane 2) were run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.The SDS-PAGE gel result shows that more than 80% biotinylated nucleosomes (H2A.Bbd) are pulled down by streptavidin beads. * indicates streptavidin.)
Application Data (Recombinant Mononucleosomes (H2A.X) - biotinylated, DNA gel. Biotinylated mononucleosomes (H2A.X) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes.Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes.)
SDS-PAGE (Streptavidin pull-down of Recombinant Mononucleosomes (H2A.X) - biotinylated. Mononucleosomes (H2A.X) were pulled down with Streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes pulled down by Streptavidin (Lane 2) were run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. The gel result shows that almost all biotinylated mononucleosomes (H2A.X) are pulled down by Streptavidin beads. *Indicates Streptavidin. Nucleosomes = Mononucleosomes)
SDS-PAGE (Recombinant Mononucleosomes (H2A.X) - biotinylated, SDS-PAGE. 12.5% SDS-PAGE gel stained with Coomassie Blue. Purity: ? 95%)
SDS-PAGE (Recombinant hnRNPA2B1-B1 activity. Biotinylated m6A ssDNA oligos were incubated with hnRNPA2B. DNA-hnRNPA2B1 compounds were pulled-down with streptavidin beads and run on SDS-PAGE gel, stained with Copmassie Blue. Results show that hnRNPA2B1 slightly preferentially binds to ssDNA with m6A modification compared with unmodified ssDNA.)
SDS-PAGE (Recombinant hnRNPA2B1-B1 protein, SDS-PAGE gel. Recombinant hnRNPA2B1-B1 was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 38.4 kDa Purity: > 90%)
SDS-PAGE (Binding Assay for Recombinant YTHDF1 protein. 45 pmol biotinylated m6A ssDNA oligos (5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with YTHDF1 protein in binding buffer for 3 hr at 4C. DNA-YTHDF1 compounds were incubated with streptavidin beads for 1 hour at 4C, and pull-down products were were run on a 10% SDS-PAGE gel, stained with Coomassie Blue. (Biotinylated,unmethylated ssDNA oligos used as negative control). YTHDF1 preferentially binds to ssDNA with m6A modifications compared with unmodified ssDNA.)
Application Data (HTRF Assay for Recombinant YTHDF1 protein activity. 3 uM m6A ssDNA oligos (DNA sequence: 5’-GTTGG/ m6A/CTT-3’) were incubated with YTHDF1 protein in binding buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SDS-PAGE (Recombinant YTHDF1 protein gel. Recombinant YTHDF1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 62.1 kDa Purity: > 80%)
Application Data (SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 ug/mL RBD, aa 319-589 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 ug/mL RBD, aa 319-589. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.)
SDS-PAGE (Recombinant SARS-CoV-2 Spike protein, S1 (aa319-589) protein gel. 1 ug of SARS-CoV-2 Spike protein was run on a 4-12% SDS-PAGE gel, stained with Coomassie blue. MW: 40 kDa Purity: >90%)
Application Data (HTRF assay for JAK1 (438-1154) activity 1 uM TK substrate was incubated with different concentrations of JAK1 (438-1154) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant JAK1 (438-1154) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 83.6 kDa Purity: >65%)
Application Data (HTRF assay for GSG2 (470-798) / HASPIN activity 1 uM STK S1 substrate was incubated with different concentrations of GSG2 (470-798) / HASPIN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for GSG2 (470-798) / HASPIN activity 1 uM STK S1 substrate was incubated with different concentrations of GSG2 (470-798) / HASPIN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant GSG2 (470-798) / HASPIN protein 10% SDS-PAGE gel with Coomassie blue staining MW: 38.7 kDa Purity: >80%)
ELISA (Bovine interleukin-6 ELISA: Recombinant Bovine interleukin-6 detected using Chicken anti Bovine interleukin-6 as the capture reagent and Chicken anti Bovine interleukin-6:Biotin as the detection reagent followed by Streptavidin:HRP)
Application Data (GTPase-Glo assay for HRAS (1-186) activity 1uM rGTP and 0.5 mM DTT was incubated with different concentrations of HRAS (1-186) in GTPase/GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5ul. To the completed GTPase reactions, we added 5ul of reconstituted GTPase-Glo Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10ul) was added, reactions were incubated for 10 minutes and luminescence was measured.)
SDS-PAGE (Recombinant MRM1-MBD protein 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 22.06kDa Purity: >95%)
Application Data (HTRF assay for EPHA4 (570-986) activity 1 uM TK substrate was incubated with different concentrations of EPHA4 (570-986) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for EPHA4 (570-986) activity 1 uM TK substrate was incubated with different concentrations of EPHA4 (570-986) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant EPHA4 (570-986) protein gel. 10% SDS-PAGE gel stained with Coomassie Blue. MW: 48.46kDa Purity: >95%)
Application Data (AMP-Glo assay for UBE2L3 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2L3 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data (AMP-Glo assay for UBE2D1 activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2D1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
Application Data (HTRF assay for ErbB3/HER3 (665-1342) activity 1uM TK substrate was incubated with different concentrations of ErbB3 (665-1342) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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