Application Data
(HTRF assay for ErbB3/HER3 (665-1342) activity 1uM TK substrate was incubated with different concentrations of ErbB3 (665-1342) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
ErbB3/HER3 recombinant protein
Recombinant ErbB3/HER3 (665-1342) protein
Synonyms
ErbB3/HER3; N/A; Recombinant ErbB3/HER3 (665-1342) protein; Receptor tyrosine protein kinase erbB-3; HER3; Oncogene Homolog 3; LCCS2; FERLK; ErbB-3; ErbB3-S; EC 2.7.10; ErbB3/HER3 recombinant protein
Host
Baculovirus
Form/Format
Recombinant ErbB3/HER3 (665-1342) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant ErbB3/HER3 (665-1342) protein that includes amino acids 665-1342 of human ErbB3 protein (accession number NP_001973.2) was expressed in a baculovirus expression system and contains an N-terminal DYKDDDDK tag. The molecular weight of the protein is 76.52kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF assay for ErbB3/HER3 (665-1342) activity 1uM TK substrate was incubated with different concentrations of ErbB3 (665-1342) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for ErbB3/HER3 (665-1342) activity 1uM TK substrate was incubated with different concentrations of ErbB3 (665-1342) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant ErbB3/HER3 (665-1342) protein 10% SDS-PAGE Comassie staining MW: 76.52kDa Purity: >95%)
SDS-PAGE
(Recombinant ErbB3/HER3 (665-1342) protein 10% SDS-PAGE Comassie staining MW: 76.52kDa Purity: >95%)
Related Product Information for ErbB3/HER3 recombinant protein
ErbB3 (Receptor tyrosine-protein kinase erbB-3) is a tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation.
Product Categories/Family for ErbB3/HER3 recombinant protein
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Product Notes
The ErbB3/HER3 (Catalog #AAA60409) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ErbB3/HER3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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