Application Data
(This assay recognizes both recombinant and natural mouse ErbB3. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50ng/mL of recombinant human ErbB3 reads as 0.7ng/mL(1.4% cross-reactivity).A sample containing 50ng/mL of recombinant rat ErbB3 reads as 3.8ng/mL(7.5% cross-reactivity).A sample containing 50ng/mL of recombinant Cynomolgus ErbB3 reads as 0.1ng/mL(0.2% cross-reactivity).)
Mouse HER3/ErbB3 ELISA Kit | HER3/ErbB3 elisa kit
Mouse HER3/ErbB3 ELISA Kit
Reactivity
Mouse
Synonyms
HER3/ErbB3; N/A; Mouse HER3/ErbB3 ELISA Kit; HER3/ERBB3 ELISA Kit, Mouse; erb-b2 receptor tyrosine kinase 3; C76256 ELISA Kit, Mouse; Erbb-3 ELISA Kit, Mouse; Erbb-3r ELISA Kit, Mouse; Erbb3 ELISA Kit, Mouse; Erbb3r ELISA Kit, Mouse; Her3 ELISA Kit, Mouse; HER3/ErbB3 elisa kit
Reactivity
Mouse
Specificity
For the quantitative determination of Mouse HER3/ErbB3 concentration in serum.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum
Detection Range
39-2500pg/mL
Sensitivity
28pg/mL
Conjugation
HRP
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural mouse ErbB3. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50ng/mL of recombinant human ErbB3 reads as 0.7ng/mL(1.4% cross-reactivity).A sample containing 50ng/mL of recombinant rat ErbB3 reads as 3.8ng/mL(7.5% cross-reactivity).A sample containing 50ng/mL of recombinant Cynomolgus ErbB3 reads as 0.1ng/mL(0.2% cross-reactivity).)
Application Data
(This assay recognizes both recombinant and natural mouse ErbB3. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50ng/mL of recombinant human ErbB3 reads as 0.7ng/mL(1.4% cross-reactivity).A sample containing 50ng/mL of recombinant rat ErbB3 reads as 3.8ng/mL(7.5% cross-reactivity).A sample containing 50ng/mL of recombinant Cynomolgus ErbB3 reads as 0.1ng/mL(0.2% cross-reactivity).)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for HER3/ErbB3 elisa kit
This HER3/ERBB3 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HER3/ERBB3 protein in Serum. It contains recombinant Mouse HER3/ERBB3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
Background: ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
References
Kraus M.H., et al., (1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
Katoh M., et al., (1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
Katoh M., et al., (1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
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Product Notes
The Mouse HER3/ErbB3 (Catalog #AAA258492) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258492 ELISA Kit recognizes Mouse HER3/ErbB3. It is sometimes possible for the material contained within the vial of "HER3/ErbB3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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