Rabbit anti-Human TRIM21 Polyclonal Antibody | anti-TRIM21 antibody

TRIM21 Antibody

Cat. Num. AAA40531

• Gene Names: TRIM21; SSA; RO52; SSA1; RNF81; Ro/SSA
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: TRIM21 antibody is affinity chromatography purified via peptide column.
• Synonyms: TRIM21, Antibody; TRIM21 Antibody; TRIM21; SSA; RO52; SSA1; RNF81; Ro/SSA; E3 ubiquitin-protein ligase TRIM21; 52 kDa Ro protein; SS-A; tripartite motif containing 21; anti-TRIM21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
• Purity/Purification: TRIM21 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 475

• Applicable Applications for anti-TRIM21 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: The immunogen is located within amino acids 290-340 of TRIM21.
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (73%), Mouse (79%)
• Preparation and Storage: TRIM21 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of TRIM21 in human small intestine tissue with TRIM21 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of TRIM21 in human small intestine tissue with TRIM21 antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of TRIM21 in human lung tissue lysate with TRIM21 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-TRIM21 antibody

TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1). RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2). TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3).

NCBI and Uniprot Product Information

• NCBI GI #: 15208660
• NCBI GeneID: 6737
• NCBI Accession #: NP_003132
• NCBI GenBank Nucleotide #: NM_003141.3
• UniProt Accession #: P19474; Q5XPV5; Q96RF8
• Molecular Weight: Predicted: 52 kDa; ; Observed: 52 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM21
• NCBI Official Synonym Full Names: tripartite motif containing 21
• NCBI Official Symbol: TRIM21
• NCBI Official Synonym Symbols: SSA; RO52; SSA1; RNF81; Ro/SSA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; RING finger protein 81; Sicca syndrome antigen A; tripartite motif-containing 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM21
• UniProt Gene Name: TRIM21
• UniProt Synonym Gene Names: RNF81; RO52; SSA1; SS-A
• UniProt Entry Name: RO52_HUMAN