IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using TRIM21 Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit anti-Human TRIM21 Polyclonal Antibody | anti-TRIM21 antibody
TRIM21 Polyclonal Antibody
Gene Names
TRIM21; SSA; RO52; SSA1; RNF81; Ro/SSA
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
TRIM21, Antibody; TRIM21 Polyclonal Antibody; RNF81; RO52; SSA; SSA1; anti-TRIM21 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
475
Applicable Applications for anti-TRIM21 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human TRIM21
Calculated Molecular Weight
54kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using TRIM21 Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using TRIM21 Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TRIM21 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TRIM21 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-TRIM21 antibody
TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle, which composed of a single polypeptide and 1 of 4 small RNA molecules.
Product Categories/Family for anti-TRIM21 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
475
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM21
NCBI Official Synonym Full Names
tripartite motif containing 21
NCBI Official Symbol
TRIM21
NCBI Official Synonym Symbols
SSA; RO52; SSA1; RNF81; Ro/SSA
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; RING finger protein 81; Sicca syndrome antigen A; tripartite motif-containing 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM21
UniProt Gene Name
TRIM21
UniProt Synonym Gene Names
RNF81; RO52; SSA1; SS-A
UniProt Entry Name
RO52_HUMAN
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Product Notes
The TRIM21 trim21 (Catalog #AAA36815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM21 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM21 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TRIM21 trim21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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