IHC (Immunohiostchemistry)
(p16INK4a/CDKN2A Antibody-Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human p16INK4a/CDKN2A Polyclonal Antibody | anti-p16INK4a/CDKN2A antibody
p16INK4a/CDKN2A Rabbit anti-Human Polyclonal (aa1-60) Antibody
Reactivity
Human
Applications
Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
p16INK4a/CDKN2A, Antibody; p16INK4a/CDKN2A Rabbit anti-Human Polyclonal (aa1-60) Antibody; PathPlus p16INK4a/CDKN2A Antibody (aa1-60); INK4A; Multiple tumor suppressor 1; MTS-1; MTS1; p16-INK4A; p16 INK4A; p16INK4A; CDKN2A; cyclin-dependent kinase inhibitor 2A; anti-p16INK4a/CDKN2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody recognizes human p16INK4a protein transcribed from gene CDKN2A (NP_000068.1, P42771).
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.1% Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-p16INK4a/CDKN2A antibody
IHC (Immunohistochemistry)
Target
Human p16INK4a/CDKN2A
Immunogen
Peptide from within AA 1-60 of human p16ink4a/CDKN2A (P42771).
Conjugation
Unconjugated
Epitope
aa1-60
Preparation and Storage
Aliquot and store undiluted at -20 degree C or below for up to 1 year. Can be stored undiluted at 4 degree C for up to 1 month. Avoid freeze-thaw cycles.
IHC (Immunohiostchemistry)
(p16INK4a/CDKN2A Antibody-Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(p16INK4a/CDKN2A Antibody-Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(p16INK4a/CDKN2A Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(p16INK4a/CDKN2A Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-p16INK4a/CDKN2A antibody
CDKN2A antibody is an unconjugated rabbit polyclonal antibody to CDKN2A (p16INK4a)(aa1-60) from human.
p16 (p16ink4a) is one of multiple independent proteins transcribed from the gene CDKN2A. p16 can prevent phosphorylation of Rb protein (RB1) by inhibiting CDK4 and CDK6, and it manages the G1 to S transition in the cell cycle via this p16-Rb pathway. Furthermore, expression of p16 is involved in regulating cellular senescence. Cancers see frequent mutation, deletion and hypermethylation of CDKN2A, or alternatively may show overexpression of transcribed p16 protein alongside knockout of its repressor RB1. Thus, both up- and down-regulation of p16 as part of the dysregulation of the p16-Rb pathway can be indicative of loss of senescent properties and a breakdown of the negative regulation of proliferation in the cell. In immunohistochemistry, p16 antibodies are frequently used when examining anogenital lesions and in the identification of HPV-associated precancerous lesions. Overexpression correlates with oncogenic HPV infection, particularly alongside loss of RB expression. Typical positive staining of p16 is described as a "block" of ten to twenty cells that together show strong nuclear and cytoplasmic expression. p16 is also positive in squamous cell carcinoma, Mullerian serous carcinoma, and head and neck carcinoma, and high expression is correlated with high grade cancers. Lastly, it should be noted that p16-specific antibodies do not cross-react with p14ARF (also from CDKN2A), as these two proteins are transcribed from alternative open reading frames.
p16 (p16ink4a) is one of multiple independent proteins transcribed from the gene CDKN2A. p16 can prevent phosphorylation of Rb protein (RB1) by inhibiting CDK4 and CDK6, and it manages the G1 to S transition in the cell cycle via this p16-Rb pathway. Furthermore, expression of p16 is involved in regulating cellular senescence. Cancers see frequent mutation, deletion and hypermethylation of CDKN2A, or alternatively may show overexpression of transcribed p16 protein alongside knockout of its repressor RB1. Thus, both up- and down-regulation of p16 as part of the dysregulation of the p16-Rb pathway can be indicative of loss of senescent properties and a breakdown of the negative regulation of proliferation in the cell. In immunohistochemistry, p16 antibodies are frequently used when examining anogenital lesions and in the identification of HPV-associated precancerous lesions. Overexpression correlates with oncogenic HPV infection, particularly alongside loss of RB expression. Typical positive staining of p16 is described as a "block" of ten to twenty cells that together show strong nuclear and cytoplasmic expression. p16 is also positive in squamous cell carcinoma, Mullerian serous carcinoma, and head and neck carcinoma, and high expression is correlated with high grade cancers. Lastly, it should be noted that p16-specific antibodies do not cross-react with p14ARF (also from CDKN2A), as these two proteins are transcribed from alternative open reading frames.
References
Park, Ben Ho et al. Holland-Frei Cancer Medicine. 6th edition. Hamilton (ON): BC Decker; 2003, URL: https://www.ncbi.nlm.nih.gov/books/NBK13507/; Int J Cancer. 2012 Apr 15;130(8):1715-25, PMID: 22025288; Cancer Res. 1994 Dec 1;54(23):6078-82, PMID: 7954450; Hum Pathol. 2017 Aug;66:144-151, PMID: 28705710
Similar Products
Product Notes
The p16INK4a/CDKN2A (Catalog #AAA162953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p16INK4a/CDKN2A Rabbit anti-Human Polyclonal (aa1-60) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p16INK4a/CDKN2A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the p16INK4a/CDKN2A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p16INK4a/CDKN2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.