CDKN2A / p16INK4a Monoclonal Antibody | anti-CDKN2A antibody

Mouse Monoclonal [clone 1E12E10] (IgG1) to Human CDKN2A / p16INK4a

Cat. Num. AAA51871

• Gene Names: CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Affinity Purified from ascites.
• Synonyms: CDKN2A / p16INK4a, Antibody; Mouse Monoclonal [clone 1E12E10] (IgG1) to Human CDKN2A / p16INK4a; Anti-CDKN2A / p16INK4a Antibody (clone 1E12E10) IHC-plus; CDKN2A; ARF; Cdk4 inhibitor; CDK4 inhibitor p16-INK4; INK4; INK4A; Multiple tumor suppressor 1; MTS-1; MTS1; p16-INK4; p16-INK4A; p16INK4; p19; p14; p16; p16 INK4A; p16INK4A; p14ARF; TP16; CDK4I; CDKN2; CMM2; MLM; p19ARF; Human CDKN2A; p16INK4a; anti-CDKN2A antibody

• Host: p16INK4a antibody was produced in Mouse.
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1E12E10
• Specificity: Targets P16. Shows reactivity with Human, Mouse, and Rat samples.
• Purity/Purification: Affinity Purified from ascites.
• Form/Format: PBS, 0.1% Sodium Azide, 1% BSA
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 156

• Applicable Applications for anti-CDKN2A antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: p16INK4a
• Immunogen Species: p16INK4a was raised against Human.
• Immunogen: p16INK4a antibody was raised against full length recombinant p16INK4a NP_000068
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Short term store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(Anti-p16INK4A antibody IHC of human colon, crypt. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-CDKN2A antibody

Found to be homozygously deleted at high frequency in cell lines derived from tumors of lung, breast, brain, bone, skin, bladder, kidney, ovary, and lymphocyte. Mutations are also associated with pancreatic adenocarcinoma.

NCBI and Uniprot Product Information

• NCBI GI #: 4502749
• NCBI GeneID: 1029
• NCBI Accession #: NP_000068.1
• NCBI GenBank Nucleotide #: NM_000077.4
• UniProt Accession #: P42771; O95440; Q15191; Q5VVJ5; Q96B52; Q9NP05; A5X2G7; D3DRK1; Q8N726
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 2A isoform p16INK4a
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2A
• NCBI Official Symbol: CDKN2A
• NCBI Official Synonym Symbols: ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
• NCBI Protein Information: cyclin-dependent kinase inhibitor 2A; CDKN2A; CDK4 inhibitor p16-INK4; multiple tumor suppressor 1; cell cycle negative regulator beta; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
• UniProt Gene Name: CDKN2A
• UniProt Synonym Gene Names: CDKN2; MTS1; CDK4I; MTS-1; p16-INK4; p16INK4A
• UniProt Entry Name: CD2A1_HUMAN