FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human p16INK4A Monoclonal Antibody | anti-p16INK4A antibody
p16INK4A Antibody
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry)
(ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
NCBI and Uniprot Product Information
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Product Notes
The p16INK4A cdkn2a (Catalog #AAA30079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p16INK4A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p16INK4A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the p16INK4A cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p16INK4A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is Rabbit anti-Human p16INK4A Monoclonal Antibody?
This antibody is a rabbit-derived monoclonal tool designed to qualitatively identify the p16INK4A antigen in formalin-fixed, paraffin-embedded (FFPE) tissue sections. As a tumor suppressor protein, p16INK4A is a critical regulator of the G1/S cell cycle checkpoint. AAA Biotech offers this antibody for research into cellular senescence and various cancers where p16 expression is altered.
How does it work?
The antibody works by binding specifically to the p16INK4A protein, which inhibits cyclin-dependent kinases like CDK4 and CDK6. By preventing these kinases from phosphorylating the retinoblastoma (Rb) protein, p16INK4A helps halt the cell cycle. Researchers use this binding to visualize the protein's presence and distribution within cells via microscopy.
What are its main applications?
The primary application is immunohistochemistry (IHC) for identifying p16 overexpression, which is a hallmark of certain viral-induced cancers, such as cervical squamous cell carcinoma. It is also utilized in Western Blotting and immunofluorescence assays to study cell cycle regulation and tumor suppression pathways in human tissue samples and cell lines.
Why choose this antibody over others?
Choosing the AAA Biotech monoclonal version ensures high specificity and superior batch-to-batch consistency compared to polyclonal alternatives. Monoclonal antibodies reduce background staining and provide more reliable results in demanding IHC protocols. Its high affinity for the p16INK4A epitope makes it a dependable choice for detecting low-level protein expression in complex tissue environments.
How should it be stored and handled?
This antibody should generally be stored at -20°C for long-term stability, though some ready-to-use formats may be kept at 2-8°C. It is important to aliquot the concentrated form upon receipt to avoid repeated freeze-thaw cycles, which can degrade the protein. Always handle with standard laboratory safety precautions and use within the recommended expiration date.
What should be considered when selecting it?
When selecting this antibody, researchers should consider the specific tissue type and the validated application, such as IHC-P or Western Blot. It is essential to include appropriate positive controls, like cervical cancer tissue, and negative controls to ensure staining accuracy. AAA Biotech recommends reviewing the datasheet for the optimal dilution ratio tailored to your experimental setup.
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