Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (SPRY2 monoclonal antibody Western Blot analysis of SPRY2 expression in C32)
Application Data (Detection limit for recombinant GST tagged SPRY2 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody Lane 1: SPRY2 transfected lysate (34.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (60.76kD).)
WB (Western Blot) (CDC2 monoclonal antibody, Western Blot analysis of CDC2 expression in Hela.)
Application Data (Detection limit for recombinant GST tagged CDC2 is ~30ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3ug/ml].)
WB (Western Blot) (Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody. Lane 1: CDC2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (58.41kD).)
Application Data (Detection limit for recombinant GST tagged MEOX2 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEOX2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml])
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in Hela NE.)
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in PC-12.)
WB (Western Blot) (MEOX2 monoclonal antibody Western Blot analysis of MEOX2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (59.07kD).)
Application Data (Detection limit for recombinant GST tagged HIP1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HIP1 transfected lysate using HIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIP1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody Lane 1: HIP1 transfected lysate (116.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HIP1 monoclonal antibody Western Blot analysis of HIP1 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (IL20 monoclonal antibody, Western Blot analysis of IL20 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged IL20 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of IL20 transfected lysate using IL20 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL20 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of IL20 expression in transfected 293T cell line by IL20 monoclonal antibody Lane 1: IL20 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (IL20 monoclonal antibody. Western Blot analysis of IL20 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-YY1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YY1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YY1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YY1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YY1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of YY1 on different lysates using anti-YY1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HL-60)
ICC (Immunocytochemistry) (ICC staining Calmodulin in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calmodulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calmodulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western Blot analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody,. Lane 1: USF2 transfected lysate (37kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in different cell lines.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1ug/ml])
Application Data (Detection limit for recombinant GST tagged USF2 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in PC-12.)
WB (Western Blot) (USF2 monoclonal antibody Western Blot analysis of USF2 expression in HeLa.)
USF2 (Upstream Stimulatory Factor 2, Class B Basic Helix-loop-helix Protein 12, bHLHb12, FOS-interacting Protein, FIP, Major Late Transcription Factor 2, Upstream Transcription Factor 2) (FITC)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)
WB (Western Blot) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody.Lane 1: CDADC1 transfected lysate (58.5kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1)
Gene Names
CDADC1; NYD-SP15; bA103J18.1; RP11-103J18.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified Purified by Protein A affinity chromatography.
Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS7 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in NIH/3T3.)
WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in Raw 264.7.)
WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in PC-12.)
WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in HeLa.)
IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (DCUN1D1 monoclonal antibody, Western Blot analysis of DCUN1D1 expression in HepG2.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCUN1D1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of DCUN1D1 expression in transfected 293T cell line by DCUN1D1 monoclonal antibody. Lane 1: DCUN1D1 transfected lysate (30.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in NIH/3T3.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
DCUN1D1 (DCN1-like Protein 1, DCUN1 Domain-containing Protein 1, Defective in Cullin Neddylation Protein 1-like Protein 1, Squamous Cell Carcinoma-related Oncogene, DCUN1L1, RP42, SCCRO) (HRP)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody. Lane 1: GMNN transfected lysate (23.6kD). Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue))
Application Data (Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.)
WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (75.68kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Detection limit for recombinant GST tagged SDCBP is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SDCBP transfected lysate using SDCBP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SDCBP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SDCBP on HepG2 cell. [antibody concentration 35ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SDCBP on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SDCBP expression in transfected 293T cell line by SDCBP monoclonal antibody. Lane 1: SDCBP transfected lysate (32.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SDCBP monoclonal antibody, Western Blot analysis of SDCBP expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD4 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD4 and StarBright Violet 610 conjugated Rat anti Mouse CD3 . All experiments performed on red blood lys)
Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV440 conjugated Rat anti Mouse CD4 . All experiments performed on mouse blood gated on live sin)
Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV515 conjugated Rat anti Mouse CD4 . All experiments performed on red cell lysed mouse blood ga)
Application Data (Figure A. FITC conjugated Rat anti Mouse CD4 . Figure B. FITC conjugated Rat anti Mouse CD4 and SBV515 conjugated Rat anti Mouse CD3 . All experiments performed on red cell lysed mouse blood gated on live single cell lym)
Application Data (Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)
Application Data (Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat anti Mouse CD4 . All expe)
Application Data (Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat anti Mouse CD4 . All exper)
Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Rat anti Mou)
Application Data (Figure A. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat anti Mouse CD4 . All experiments pe)
Application Data (Figure A. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)
WB (Western Blot) (Western Blot analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody. Lane 1: ACY1 transfected lysate (45.9kD). Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of ACY1 transfected lysate using ACY1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ACY1 rabbit polyclonal antibody.)
WB (Western Blot) (ACY1 monoclonal antibody Western Blot analysis of ACY1 expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (70.62kD).)
WB (Western Blot) (IL20 monoclonal antibody, Western Blot analysis of IL20 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged IL20 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of IL20 transfected lysate using IL20 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL20 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of IL20 expression in transfected 293T cell line by IL20 monoclonal antibody Lane 1: IL20 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (IL20 monoclonal antibody. Western Blot analysis of IL20 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)
WB (Western Blot) (NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.)
Application Data (Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody. Lane 1: NEK11 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (79.13kD).)
Application Data (Detection limit for recombinant GST tagged PIK3R4 is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.6 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.6 ug/ml])
WB (Western Blot) (PIK3R4 monoclonal antibody (M02), clone 1B5 Western Blot analysis of PIK3R4 expression in HeLa (Cat # L013V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIK3R4 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIK3R4 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA27028 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with AAA27028 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IHC (Immunohistochemistry) (IHC image of AAA27028 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA27028 diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry) (IHC image of AAA27028 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA27028 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA27028 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA27028 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysateAll lanes: CD14 antibody at 1:1800SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDa)
WB (Western Blot) (Western BlotPositive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissueAll lanes: CD14 antibody at 1:2500SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDa)
ICC (Immunocytochemistry) (ICC staining NUMB in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NUMB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NUMB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NUMB antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using anti-NUMB antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NUMB antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NUMB on A431 cells lysates using anti-NUMB antibody at 1/1, 000 dilution.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in Raw 264.7 (Cat # L024V1).)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in PC-12 (Cat # L012V1).)
Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10 Western Blot analysis of FGR expression in HeLa (Cat # L013V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (YY1 monoclonal antibody (M03), clone 4D2 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
WB (Western Blot) (Figure 8. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat lung tissue lysates,Lane 4: mouse heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse lung tissue lysates,Lane 7: rat RH35 whole cell lysates,Lane 8: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19718) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U937 cells using anti-Cyclophilin E/PPIE antibody (AAA19718).Overlay histogram showing U937 cells stained with AAA19718 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin E/PPIE Antibody (AAA19718, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-Cyclophilin E/PPIE antibody (AAA19718).Overlay histogram showing JK cells stained with AAA19718 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin E/PPIE Antibody (AAA19718, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 5. IF analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19718) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)
IHC (Immunohistchemistry) (THY1/CD90 Antibody-IHC of paraffin-embedded endometrial cancer tissues using THY1 mouse monoclonal antibody with DAB staining.)
IHC (Immunohistochemistry) (THY1/CD90 Antibody-IHC of paraffin-embedded cerebellum tissues using THY1 mouse monoclonal antibody with DAB staining.)
WB (Western Blot) (THY1/CD90 Antibody-Western blot using THY1 monoclonal antibody against human THY1 recombinant protein. (Expected MW is 38.5 kDa))
WB (Western Blot) (THY1/CD90 Antibody-Western blot using THY1 monoclonal antibody against HEK293 (1) and THY1 (AA: 17-132)-hIgGFc transfected HEK293 (2) cell lysate.)
WB (Western Blot) (THY1/CD90 Antibody-Western blot using THY1 mouse monoclonal antibody against T47D (1), HepG2 (2) and PC-12 (3) cell lysate.)
ICC (Immunocytochemistry) (THY1/CD90 Antibody-Immunofluorescence of HeLa cells using THY1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml])
WB (Western Blot) (Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.36kD).)
WB (Western Blot) (Western blot analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PCDH8 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody. Lane 1: PCDH8 transfected lysate (113kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCDH8 monoclonal antibody Western Blot analysis of PCDH8 expression in COLO 320 HSR.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and MDM2 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
WB (Western Blot) (Western blot analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MDM2 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5ug/ml])
WB (Western Blot) (Western Blot analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody. Lane 1: MDM2 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of FOXA1 over-expressed 293 cell line, cotransfected with FOXA1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA1 expression in transfected 293T cell line by FOXA1 monoclonal antibody Lane 1: FOXA1 transfected lysate (49.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA1 monoclonal antibody Western Blot analysis of FOXA1 expression in HepG2)
WB (Western Blot) (Western Blot detection against Immunogen (37.4kD).)
FCM (Flow Cytometry) (Overlay histogram showing MCF-7 cells stained with (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1/200 for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of PC-3 cells at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG ?H+L?.)
IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG ?H+L?.)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG ?H+L?.)
IHC (Immunohistochemistry) (IHC image diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot) (Western Blot Positive WB detected in: 20ug hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug All lanes: HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5min)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Raji cells with PAX5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PAX5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PAX5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PAX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PAX5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PAX5 on Daudi cells lysates using anti-PAX5 antibody at 1/1, 000 dilution.)
SDS-PAGE (SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8(ABT536) antibody. The HRP-conjugated Goat anti-M)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FAK on different lysates using anti-FAK antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen)
IF (Immunofluorescence) (ICC/IF analysis of ISG15 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ISG15 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.)
IF (Immunofluorescence) (Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).)
WB (Western Blot) (Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).)
WB (Western Blot) (Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CITED1 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of CITED1 expression in transfected 293T cell line by CITED1 monoclonal antibody. Lane 1: CITED1 transfected lysate (19.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CITED1 monoclonal antibody Western Blot analysis of CITED1 expression in A-431.)
WB (Western Blot) (CITED1 monoclonal antibody. Western Blot analysis of CITED1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (CAMKK1 monoclonal antibody, Western Blot analysis of CAMKK1 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged CAMKK1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody. Lane 1: CAMKK1 transfected lysate (57.31kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (83.31kD).)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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