Standard Curve (Sample)
Rat secreted phosphoprotein 1 ELISA Kit | SPP1 elisa kit
Rat Osteopontin, OPN ELISA Kit
Gene Names
SPP1; OPN
Reactivity
Rat
Synonyms
secreted phosphoprotein 1; N/A; Rat Osteopontin, OPN ELISA Kit; Rat Osteopontin; OPN ELISA Kit; BNSP; BSPI; ETA-1; MGC110940; OPN; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; s; SPP1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat SPP1. No significant cross-reactivity or interference between Rat SPP1 and analogues was observed.
Sequence Length
314
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 3
Biomineralization
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPP1 elisa kit
Introduction: Osteopontin is a glycoprotein that was first identified in 1986 in osteoblasts. Osteopontin is an extracellular structural protein and therefore an organic component of bone. Synonyms for this protein include sialoprotein I and 44K BPP (bone phosphoprotein). Osteopontin is biosynthesized by a variety of tissue types including fibroblasts preosteoblasts, osteoblasts, osteocytes, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, dendritic cells, macrophages, smooth muscle, skeletal muscle myoblasts, endothelial cells, and extraosseous(non-bone) cells in the inner ear, brain, kidney, deciduum, and placenta. Synthesis of osteopontin is stimulated by calcitriol (1,25-dihydroxy-vitamin D3). The protein is composed of about 300aa residues (297 in mouse; 314 in human) and has ~30 carbohydrate residues attached including 10 sialic acid residues, which are attached to the protein during post-translational modification in the Golgi apparatus. The protein is rich in acidic residues: 30-36% are either aspartic or glutamic acid. OPN is a highly negative charged, extracellular matrix protein that lacks an extensive secondary structure. OPN can go through posttranslational modifications which increase its apparent molecular weight to about 44 kDa.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OPN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for OPN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OPN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OPN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for OPN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OPN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,172 Da
NCBI Official Full Name
osteopontin
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN
NCBI Protein Information
osteopontin; OC-1; SPP-1; bone sialoprotein 1; secreted phosphoprotein-1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
UniProt Protein Name
Osteopontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
SPP-1
UniProt Entry Name
OSTP_RABIT
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Product Notes
The Rat SPP1 spp1 (Catalog #AAA80540) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80540 ELISA Kit recognizes Rat SPP1. It is sometimes possible for the material contained within the vial of "secreted phosphoprotein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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