IHC (Immunohiostchemistry)
Rat Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Monoclonal Antibody | anti-SPP1 antibody
Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Rat Monoclonal Antibody [Clone OSP/4589]
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Functional Assay
Synonyms
Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1), Antibody; Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Rat Monoclonal Antibody [Clone OSP/4589]; Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1); BNSP; Bone sialoprotein 1 (BSPI/BSP1); Early T lymphocyte activation 1 (ETA1); Nephropontin; immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); CALPHA1 fusion; Urinary stone protein; Uropontin; anti-SPP1 antibody
Host
Rat
Reactivity
Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.
Clonality
Monoclonal
Isotype
Rat/IgG1
Clone Number
OSP/4589
Form/Format
Supplied in 1.0mg/ml with out BSA & Azide.
Applicable Applications for anti-SPP1 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), FA (Functional Assay)
Immunogen
Recombinant full-length rat Spp1 protein
Cellular Localization
Secreted
Positive Control
Bone. Found in plasma.
Hu-chromosome Location
4q22.1
Specificity
Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
Preparation and Storage
Antibody without azide: store at -20 to -80 degree C. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
IHC (Immunohiostchemistry)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human brain stained with Osteopontin Rat Monoclonal Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human brain stained with Osteopontin Rat Monoclonal Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34.9 kDa
NCBI Official Full Name
osteopontin isoform OPN-b
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I
UniProt Protein Name
Osteopontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN
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Product Notes
The SPP1 spp1 (Catalog #AAA215943) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Rat Monoclonal Antibody [Clone OSP/4589] reacts with Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey. and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), FA (Functional Assay). Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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