Search results for " Abs to Cell Markers"

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

CD138, Monoclonal Antibody (Cat# AAA24083)

Full Name

CD138, CT (SDC1, SDC, Syndecan-1, CD_antigen=CD138, Flags: Precursor)

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunofluorescence

Purity

Ascites
Ascites

Pricing

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WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in NIH/3T3.)

Ubiquilin-2, Monoclonal Antibody (Cat# AAA24086)

Full Name

Ubiquilin-2 (UBQLN2, Chap1, DSK2 Homolog, HRIHFB2157, N4BP4, Protein Linking IAP with Cytoskeleton 2, PLIC2, PLIC-2, hPLIC-2, Ubiquitin-like Product Chap1/Dsk2)

Gene Names

UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

CITED1, Monoclonal Antibody (Cat# AAA24077)

Full Name

CITED1 (CBP/p300 Interacting Transactivator 1, Cbp/p300-interacting Transactivator with Glu/Asp-rich Carboxy-terminal Domain 1, Melanocyte-specific Protein1)

Gene Names

CITED1; MSG1

Reactivity

Human, Rat

Applications

Western Blot

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)

LETM1 and EF-hand Domain Containing Protein 1, Mitochondrial, Monoclonal Antibody (Cat# AAA14784)

Full Name

LETM1 and EF-hand Domain Containing Protein 1, Mitochondrial (LETM1, Leucine Zipper-EF-hand-containing Transmembrane Protein 1)

Reactivity

Human

Applications

EL/EIA, WB, IP, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FGFR2, Polyclonal Antibody (Cat# AAA14790)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

EL/EIA, WB, IHC, FC/FACS, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)

TIMM9, Monoclonal Antibody (Cat# AAA24059)

Full Name

TIMM9 (Mitochondrial Import Inner Membrane Translocase Subunit Tim9, TIM9, TIM9A, TIMM9A)

Reactivity

Human, Mouse, Rat

Applications

EL/EIA, WB, IHC

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14653)

Full Name

Mast Cell Chymase

Gene Names

CMA1; CYH; MCT1; chymase; MGC119890; MGC119891

Reactivity

Human

Applications

Immunohistochemistry

Purity

Purified by Protein G affinity chromatography

Pricing

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Application Data (Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 used for the evaluation of HLA class II histocompatibility antigen expression on cell populations by flow cytometry.Image caption:DC differentiation from H1 hESC.Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012;2012:172420.doi: 10.1155/2012/172420.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the treatment on monocytes in vitrodetection of MHC class II onImage caption:Ligand engagement of HLA class II molecules up regulates MyD88, IL-1R1, and TNF-? in CD14+ human monocytes treated with SEB, SEA, or mAb directed against MHC-class II molecules. (A) Transcriptional activation of MyD88, IL-1R1AcP and TNF-? in HLA-DR positive primary monocytes treated with 200 ng SEB/ml (optimum dose), mAbs (10 µg/ml, optimum dose) directed against MHC-class II molecule (anti-DR-DP-DQ or LB3.1) or unrelated control antibody OKT3 was examined by semi-quantitative RT-PCR. Data shown is one of 3 similar experiments; (B) Agonists binding to TLR4 or HLA class II molecules on CD14+ monocytes induced transcriptional up regulation of MyD88. Real time RT-PCR was used to determine relative expression of MyD88 normalized to the expression of ?-actin. Expression levels are determined as means ± SD. Data presented as one of 3 similar experiments. Significance compared to untreated control (*) was assigned as P?0.0001. (C) MHC class II molecule dependence of SEB- induced TNF- ? gene expression. Pretreatment of monocytes in ice with anti-DR-DP-DQ at optimum dose (10 ug/ml) followed by SEB stimulation resulted in reduced TNF- ? gene expression. Transcriptional activation of TNF- ? expression normalized to the expression of ?-actin. Expression levels of TNF- ? are expressed as means ± SD. Significance was assigned (*) or (**) as P values ?0.003 comparing untreated vs treatment groups with anti-DR-DP-DQ, SEB or SEB vs anti-DR-DP-DQ+SEB respectively. (D) Confocal images show expression of HLA-DR (green) and intracellular MyD88 (red) proteins in CD14+ monocytes treated with HLA class II- ligands or control antibody OKT3 for 16 h; Scale bar = 5 ?m; (E) intracellular expression of MyD88 protein in activated monocytes. Primary monocytes (CD14+, CD3-) were activated as described earlier, permeabilized and labeled with primary MyD88 antibody followed by PE-labeled secondary antibody and analyzed by flow cytometry. Histogram represents a MHC class II ligand-induced increase in expression of MyD88 protein compared to non-MHC class II ligand (OKT3).From: Kissner TL, Ruthel G, Alam S, Ulrich RG, Fernandez S, et al. (2011) Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules.PLoS ONE 6(1): e15985.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the evaluation of MHC class II expression on dendritic cells by flow cytometry.Image caption:Time course of DC differentiation from H1 hESC. Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (a) Cells harvested at day 20 of culture showing expressing of CD45 but lack of myeloid commitment, as evidenced by staining for CD13, CD14, and CD11c. Open histograms show levels of background staining using isotype-matched control antibodies. (b) Appearance of CD45int cells at day 27 of culture, accompanied by the upregulation of myeloid-specific markers. (c) Photomicrograph, taken at day 28 of culture, showing the morphology of DC, including veils of cytoplasm and long dendrites (inset) (×40 magnification). (d) Cells harvested at day 33 of culture, showing the appearance of a CD45hi population containing predominantly DC progenitors expressing CD14, CD11c, CD86 and MHC class I. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Kathryn M. Silk, Alison J. Leishman, Kevin P. Nishimoto, Anita Reddy, and Paul J. Fairchild, “Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype,”Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 172420, 11 pages, 2012.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

Full Name

Mouse Anti Human HLA DP DQ DR: Amethyst Orange

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Pricing

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CD163, Polyclonal Antibody (Cat# AAA24067)

Full Name

CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163)

Gene Names

CD163; M130; MM130

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified by ammonium sulfate precipitation.

Pricing

Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14660)

Full Name

Mast Cell Chymase (BSA & Azide Free)

Gene Names

CMA1; CYH; MCT1; chymase; MGC119890; MGC119891

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified by Protein G affinity chromatography.

Pricing

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10 results for " Abs to Cell Markers" - showing 1-10

CD138, Monoclonal Antibody (Cat# AAA24083)

Ubiquilin-2, Monoclonal Antibody (Cat# AAA24086)

CITED1, Monoclonal Antibody (Cat# AAA24077)

LETM1 and EF-hand Domain Containing Protein 1, Mitochondrial, Monoclonal Antibody (Cat# AAA14784)

FGFR2, Polyclonal Antibody (Cat# AAA14790)

TIMM9, Monoclonal Antibody (Cat# AAA24059)

Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14653)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

CD163, Polyclonal Antibody (Cat# AAA24067)

Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14660)

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Reactivity

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10 results for " Abs to Cell Markers" - showing 1-10

CD138, Monoclonal Antibody (Cat# AAA24083)

Ubiquilin-2, Monoclonal Antibody (Cat# AAA24086)

CITED1, Monoclonal Antibody (Cat# AAA24077)

LETM1 and EF-hand Domain Containing Protein 1, Mitochondrial, Monoclonal Antibody (Cat# AAA14784)

FGFR2, Polyclonal Antibody (Cat# AAA14790)

TIMM9, Monoclonal Antibody (Cat# AAA24059)

Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14653)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

CD163, Polyclonal Antibody (Cat# AAA24067)

Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14660)

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