Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
3208 results for " Protein Detection Antibody" - showing 3050-3100
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12146)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
FC/FACS
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U-87 cells using anti-ATP citrate lyase antibody (AAA11689).Overlay histogram showing U-87 cells stained with AAA11689 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP citrate lyase Antibody (AAA11689,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP citrate lyase, Polyclonal Antibody (Cat# AAA11689)
Full Name
Anti-ATP citrate lyase Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
CD25, Monoclonal Antibody (Cat# AAA11966)
Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
EIA, FC/FACS, IP
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11971)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
FC/FACS, IF, IP
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U-87 cells using anti-MVP antibody (AAA19137).Overlay histogram showing U-87 cells stained with AAA19137 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (AAA19137,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MVP, Polyclonal Antibody (Cat# AAA19137)
Full Name
Anti-MVP Picoband antibody
Gene Names
MVP; LRP; VAULT1
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-RGS6 antibody (AAA19308).Overlay histogram showing MCF-7 cells stained with AAA19308 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS6 Antibody (AAA19308, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RGS6, Polyclonal Antibody (Cat# AAA19308)
Full Name
Anti-RGS6 Antibody
Gene Names
RGS6; GAP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U87 cells using anti-GNG2 antibody (AAA19312).Overlay histogram showing U87 cells stained with AAA19312 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG2 Antibody (AAA19312, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GNG2, Polyclonal Antibody (Cat# AAA19312)
Full Name
Anti-GNG2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti- ETF/TEAD2 antibody (AAA19325).Overlay histogram showing Hela cells stained withAAA19325 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ETF/TEAD2 Antibody (AAA19325, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ETF/TEAD2, Polyclonal Antibody (Cat# AAA19325)
Full Name
Anti-ETF/TEAD2 Antibody
Gene Names
TEAD2; ETF; TEF4; TEF-4; TEAD-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of SGK1 using anti- SGK1 antibody (AAA19225).SGK1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti- SGK1 Antibody (AAA19225) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SGK1, Polyclonal Antibody (Cat# AAA19225)
Full Name
Anti-SGK1 Antibody
Gene Names
SGK1; SGK
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HELA cells using anti-MCU antibody (AAA19226).Overlay histogram showing HELA cells stained with AAA19226 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCU Antibody (AAA19226, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCU, Polyclonal Antibody (Cat# AAA19226)
Full Name
Anti-MCU Antibody
Gene Names
MCU; C10orf42; CCDC109A
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of THP-1 cells using anti-PCBP2/hnRNP E2 antibody (AAA19259).Overlay histogram showing THP-1 cells stained with AAA19259 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCBP2/hnRNP E2 Antibody (AAA19259, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCBP2/hnRNP E2, Polyclonal Antibody (Cat# AAA19259)
Full Name
Anti-PCBP2/hnRNP E2 Antibody
Gene Names
PCBP2; HNRPE2; HNRNPE2; hnRNP-E2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-ATG9A antibody (AAA19280).Overlay histogram showing Hela cells stained with AAA19280 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG9A Antibody (AAA19280, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATG9A, Polyclonal Antibody (Cat# AAA19280)
Full Name
Anti-ATG9A Antibody
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of NRK cells using anti-SHANK3 antibody (AAA19237).Overlay histogram showing NRK cells stained with AAA19237 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHANK3 Antibody (AAA19237,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SHANK3, Polyclonal Antibody (Cat# AAA19237)
Full Name
Anti-SHANK3 Antibody
Gene Names
SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ALK-1/ACVRL1 antibody (AAA19242).Overlay histogram showing MCF-7 cells stained with AAA19242 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ALK-1/ACVRL1, Polyclonal Antibody (Cat# AAA19242)
Full Name
Anti-ALK-1/ACVRL1 Antibody
Gene Names
ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-TMPRSS3 antibody (AAA19289).Overlay histogram showing A431 cells stained with AAA19289 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMPRSS3 Antibody (AAA19289, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TMPRSS3, Polyclonal Antibody (Cat# AAA19289)
Full Name
Anti-TMPRSS3 Antibody
Gene Names
TMPRSS3; DFNB8; DFNB10; ECHOS1; TADG12
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-MitoNEET/CISD1 antibody (AAA19293).Overlay histogram showing PC-3 cells stained with AAA19293 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MitoNEET/CISD1 Antibody (AAA19293,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MitoNEET/CISD1, Polyclonal Antibody (Cat# AAA19293)
Full Name
Anti-MitoNEET/CISD1 Antibody
Gene Names
CISD1; ZCD1; MDS029; C10orf70; mitoNEET
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HEPG2 cells using anti-ALDH1L1 antibody (AAA19297).Overlay histogram showing HEPG2 cells stained with AAA19297 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1L1 Antibody (AAA19297 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ALDH1L1, Polyclonal Antibody (Cat# AAA19297)
Full Name
Anti-ALDH1L1 Antibody
Gene Names
ALDH1L1; FDH; FTHFD; 10-fTHF; 10-FTHFDH
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-Transketolase/TKT antibody (AAA19368).Overlay histogram showing A549 cells stained with AAA19368 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Transketolase/TKT Antibody (AAA19368, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Transketolase/TKT, Monoclonal Antibody (Cat# AAA19368)
Full Name
Anti-Transketolase/TKT Antibody (monoclonal, 2I3)
Gene Names
TKT; TK; TKT1; HEL107
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11876)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
FC/FACS
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U-87 cells using anti-SSH3BP1 antibody (AAA11656).Overlay histogram showing U-87 cells stained with AAA11656 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3BP1 Antibody (AAA11656,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SSH3BP1, Polyclonal Antibody (Cat# AAA11656)
Full Name
Anti-SSH3BP1 Antibody
Gene Names
ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
ICC (Immunocytochemistry)
(Figure 7. IHC analysis of ERAB using anti-ERAB antibody (AAA11668).ERAB was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERAB Antibody (AAA11668) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
ERAB, Polyclonal Antibody (Cat# AAA11668)
Full Name
Anti-ERAB Antibody
Gene Names
HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
Application Data
TCP1 delta, Polyclonal Antibody (Cat# AAA11672)
Full Name
Anti-TCP1 delta Antibody
Gene Names
CCT4; SRB; Cctd; CCT-DELTA
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
CD25, Monoclonal Antibody (Cat# AAA11965)
Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
EIA, FC/FACS, IP
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Hela cells using anti-PDE4D antibody (AAA19143).Overlay histogram showing Hela cells stained with AAA19143 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4D Antibody (AAA19143,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDE4D, Polyclonal Antibody (Cat# AAA19143)
Full Name
Anti-PDE4D Picoband antibody
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11970)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
FC/FACS, IF, IP
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of MYLK using anti-MYLK antibody (AAA19154).MYLK was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA19154) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
MYLK, Polyclonal Antibody (Cat# AAA19154)
Full Name
Anti-MYLK Picoband antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
Application Data
(Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD163, Monoclonal Antibody (Cat# AAA12256)
Full Name
Mouse Anti Human CD163: RPE
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human
Applications
FC/FACS
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (AAA19291).Overlay histogram showing U937 cells stained with AAA19291 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (AAA19291, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)
Full Name
Anti-Coronin 1a/TACO/CORO1A Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of SCG10/STMN2 using anti- SCG10/STMN2 antibody (AAA19299).SCG10/STMN2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SCG10/STMN2 Antibody (AAA19299) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SCG10/STMN2, Polyclonal Antibody (Cat# AAA19299)
Full Name
Anti-SCG10/STMN2 Antibody
Gene Names
STMN2; SCG10; SCGN10
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-Glycine decarboxylase/GLDC antibody (AAA19300).Overlay histogram showing CACO-2 cells stained with AAA19300 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glycine decarboxylase/GLDC Antibody (AAA19300, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Glycine decarboxylase/GLDC, Polyclonal Antibody (Cat# AAA19300)
Full Name
Anti-Glycine decarboxylase/GLDC Antibody
Gene Names
GLDC; GCE; GCSP; HYGN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U20S cells using anti-VDAC3 antibody (AAA19301).Overlay histogram showing U20S cells stained with AAA19301 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDAC3 Antibody (AAA19301, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
VDAC3, Polyclonal Antibody (Cat# AAA19301)
Full Name
Anti-VDAC3 Antibody
Gene Names
VDAC3; VDAC-3; HD-VDAC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of 293T cells using anti-CDC45L antibody (AAA19360).Overlay histogram showing 293T cells stained with AAA19360 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- CDC45L Antibody (AAA19360, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CDC45L, Monoclonal Antibody (Cat# AAA19360)
Full Name
Anti-CDC45L Antibody (monoclonal, 6H6)
Gene Names
CDC45; CDC45L; CDC45L2; PORC-PI-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (AAA10917) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
PKR, Polyclonal Antibody (Cat# AAA10917)
Full Name
PKR Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
PKR Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19369).Overlay histogram showing SiHa cells stained with AAA19369 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19369, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ASS1, Monoclonal Antibody (Cat# AAA19369)
Full Name
Anti-ASS1 Antibody (monoclonal, 5I5)
Gene Names
ASS1; ASS; CTLN1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19370).Overlay histogram showing SiHa cells stained with AAA19370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ASS1, Monoclonal Antibody (Cat# AAA19370)
Full Name
Anti-ASS1 Antibody (monoclonal, 7I9)
Gene Names
ASS1; ASS; CTLN1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-HIF-1 alpha/HIF1A antibody (AAA19213).Overlay histogram showing A431 cells stained with AAA19213 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF-1 alpha/HIF1A Antibody (AAA19213, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HIF-1 alpha/HIF1A, Polyclonal Antibody (Cat# AAA19213)
Full Name
Anti-HIF-1 alpha/HIF1A Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (AAA19219).Overlay histogram showing HepG2 cells stained with AAA19219 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (AAA19219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MERTK, Polyclonal Antibody (Cat# AAA19219)
Full Name
Anti-MERTK Antibody
Gene Names
MERTK; MER; RP38; c-mer
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U20S cells using anti- ADA antibody (AAA19184). Overlay histogram showing U20S cells stained with AAA19184 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ADA Antibody (AAA19184, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ADA, Monoclonal Antibody (Cat# AAA19184)
Full Name
Anti-ADA Antibody (monoclonal, 6D4)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of CD72 using anti- CD72 antibody (AAA19329).CD72 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CD72 Antibody (AAA19329) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD72, Polyclonal Antibody (Cat# AAA19329)
Full Name
Anti-CD72 Antibody
Gene Names
Cd72; CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
METTL1, Polyclonal Antibody (Cat# AAA19336)
Full Name
Anti-METTL1 Antibody
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9b antibody (AAA19340).Overlay histogram showing HEPA1-6 cells stained with AAA19340 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9b Antibody (AAA19340, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rad9b, Polyclonal Antibody (Cat# AAA19340)
Full Name
Anti-Rad9b Antibody
Gene Names
Rad9b; BC021784; A630082N15Rik
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U87 cells using anti-GRB10 antibody (AAA19244).Overlay histogram showing U87 cells stained with AAA19244 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB10 Antibody (AAA19244,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GRB10, Polyclonal Antibody (Cat# AAA19244)
Full Name
Anti-GRB10 Antibody
Gene Names
GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of 293T cells using anti-TBP-1/PSMC3 antibody (AAA19314).Overlay histogram showing 293T cells stained with AAA19314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBP-1/PSMC3 Antibody (AAA19314, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TBP-1/PSMC3, Polyclonal Antibody (Cat# AAA19314)
Full Name
Anti-TBP-1/PSMC3 Antibody
Gene Names
PSMC3; TBP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of GRSF1 using anti- GRSF1 antibody (AAA19315).GRSF1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- GRSF1 Antibody (AAA19315) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GRSF1, Polyclonal Antibody (Cat# AAA19315)
Full Name
Anti-GRSF1 Antibody
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PRMT8 antibody (AAA19321).Overlay histogram showing THP-1 cells stained with AAA19321 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT8 Antibody (AAA19321,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRMT8, Polyclonal Antibody (Cat# AAA19321)
Full Name
Anti-PRMT8 Antibody
Gene Names
PRMT8; HRMT1L3; HRMT1L4
Reactivity
Human, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-REA/PHB2 antibody (AAA19374).Overlay histogram showing HepG2 cells stained with AAA19374 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- REA/PHB2 Antibody (AAA19374, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
REA/PHB2, Monoclonal Antibody (Cat# AAA19374)
Full Name
Anti-REA/PHB2 Antibody (monoclonal, 2B5)
Gene Names
PHB2; BAP; REA; p22; Bap37; BCAP37; PNAS-141
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-DDX1 antibody (AAA19379).Overlay histogram showing MCF-7 cells stained with AAA19379 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX1 Antibody (AAA19379, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DDX1, Monoclonal Antibody (Cat# AAA19379)
Full Name
Anti-DDX1 Antibody (monoclonal, 2D4)
Gene Names
DDX1; DBP-RB; UKVH5d
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
Application Data
(Figure 10 KD Validation in HeLa cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 ?M luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies (1139). DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10952)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB, ICC
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)
Ran, Polyclonal Antibody (Cat# AAA19131)
Full Name
Anti-Ran Picoband Antibody
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
Application Data
(Published Customer Image:Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?)
CD31, Monoclonal Antibody (Cat# AAA12258)
Full Name
Rat Anti Mouse CD31: FITC
Gene Names
Pecam1; Cd31; Pecam; C85791; PECAM-1
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing