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2304 results for "Secondary Antibody" - showing 200-250
FCM (Flow Cytometry)
(Flow cytometric analysis of Daudi cells with Nesprin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Nesprin 1, Monoclonal Antibody (Cat# AAA30446)
Full Name
Nesprin 1 Antibody
Gene Names
SYNE1; 8B; CPG2; ARCA1; EDMD4; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
PCNA, Polyclonal Antibody (Cat# AAA28705)
Full Name
PCNA Antibody (C-term)
Gene Names
PCNA; ATLD2
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Apolipoprotein A II, Monoclonal Antibody (Cat# AAA30454)
Full Name
Apolipoprotein A II Antibody
Gene Names
APOA2; apoAII; Apo-AII; ApoA-II
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
TCTP, Monoclonal Antibody (Cat# AAA30412)
Full Name
TCTP Antibody
Gene Names
TPT1; HRF; p02; TCTP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with ERK1/2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ERK1/2, Monoclonal Antibody (Cat# AAA29977)
Full Name
ERK1/2 Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of Tubulin alpha expression in various sample)
Tubulin alpha, Polyclonal Antibody (Cat# AAA31146)
Full Name
Tubulin alpha Antibody
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human, Mouse, Rat, Pig, Bovine, Rabbit, Chicken, Fish
Predicted: Horse, Sheep, Dog
Predicted: Horse, Sheep, Dog
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(Overlay histogram showing Raw264.7 cells stained with AAA27010 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.)
CD163, Monoclonal Recombinant Antibody (Cat# AAA27010)
Full Name
CD163 Antibody
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry
Purity
Affinity-chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with Bub3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Bub3, Monoclonal Antibody (Cat# AAA30510)
Full Name
Bub3 Antibody
Gene Names
BUB3; BUB3L; hBUB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NFAT2, Polyclonal Antibody (Cat# AAA31381)
Full Name
Phospho-NFAT2 (Ser294) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using RCC1 antibody at dilution of 1:100 (40x lens).)
RCC1, Polyclonal Antibody (Cat# AAA10777)
Full Name
RCC1 Polyclonal Antibody
Gene Names
RCC1; CHC1; RCC1-I; SNHG3-RCC1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-CTNNB1 Antibody Titration: 1.25ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)
CTNNB1, Polyclonal Antibody (Cat# AAA23610)
Full Name
CTNNB1 antibody - C-terminal region
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Protein A purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
E2F-2, Polyclonal Antibody (Cat# AAA31340)
Full Name
E2F-2 Antibody
Gene Names
E2F2; E2F-2
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-MCM4 antibody (AAA19257).Overlay histogram showing A549 cells stained with AAA19257 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM4 Antibody (AAA19257, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCM4, Polyclonal Antibody (Cat# AAA19257)
Full Name
Anti-MCM4 Antibody
Gene Names
MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(MYEF2 was immunoprecipitated using: Lane A:0.5 mg SH-SY5Y Whole Cell Lysate 4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 64 kDa Observed band size :64 kDa)
MYEF2, Polyclonal Antibody (Cat# AAA27756)
Full Name
Anti-MYEF2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD10, Monoclonal Antibody (Cat# AAA30177)
Full Name
CD10 Antibody
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.)
NLRP3, Monoclonal Antibody (Cat# AAA30151)
Full Name
NLRP3 Antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Published customer image: Effects of EDTA versus media storage of patient blood samples on flow cytometric results. Peripheral blood cells were (a) stained immediate following collection with CD11b and CADO48A or maintained in EDTA collection tubes for (b) 24 or (c) 48 hours or in media for (d) 24 or (e) 48 hours prior to staining with CD11b and CADO48A. Both EDTA and media samples were kept refrigerated. These findings show a decrease over time of population distinction in EDTA with minimal changes when cells are kept in media up to 48 hours. All cells were gated on P1.From: Sherger et al. BMC Veterinary Research 2012 8:209)
CD11b, Monoclonal Antibody (Cat# AAA12092)
Full Name
MOUSE ANTI DOG CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31377)
Full Name
p53 Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA26968 at dilution of 1:100)
STAT5B, Polyclonal Antibody (Cat# AAA26968)
Full Name
STAT5B Antibody
Gene Names
STAT5B; STAT5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Thymidine Kinase 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Thymidine Kinase 1, Monoclonal Antibody (Cat# AAA30271)
Full Name
Thymidine Kinase 1 Antibody
Gene Names
TK1; TK2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
DARPP32, Monoclonal Antibody (Cat# AAA30068)
Full Name
DARPP32 Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation of extracts of 293T cell line, using HDAC3 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
HDAC3, Polyclonal Antibody (Cat# AAA10758)
Full Name
HDAC3 Polyclonal Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
MCM2, Polyclonal Antibody (Cat# AAA31329)
Full Name
Phospho-MCM2 (Ser41) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-Caveolin-2/CAV2 antibody (AAA19243).Overlay histogram showing A549 cells stained with AAA19243 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-2/CAV2 Antibody (AAA19243,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caveolin-2/CAV2, Polyclonal Antibody (Cat# AAA19243)
Full Name
Anti-Caveolin-2/CAV2 Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2A.X, Polyclonal Antibody (Cat# AAA31320)
Full Name
Acetyl-Histone H2A.X (Lys9) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using CDKN1A antibody at dilution of 1:100 (40x lens).)
CDKN1A, Polyclonal Antibody (Cat# AAA10750)
Full Name
CDKN1A
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Fig.6. Immunofluorescence analysis of rat brain tissue. 1, GFAP Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
GFAP, Monoclonal Antibody (Cat# AAA31476)
Full Name
GFAP Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using FOXJ1 Antibody.)
FOXJ1, Polyclonal Antibody (Cat# AAA30927)
Full Name
FOXJ1 Antibody
Gene Names
FOXJ1; HFH4; HFH-4; FKHL13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SOCS1, Polyclonal Antibody (Cat# AAA28752)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
ERK1/2, Polyclonal Antibody (Cat# AAA31416)
Full Name
Phospho-ERK1/2 (Thr202+Tyr204/Thr185+Tyr187) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (AAA10650) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
H3K9me3, Antibody (Cat# AAA10650)
Full Name
Histone H3K9me3 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Dot Blot, Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NMDAR1, Monoclonal Antibody (Cat# AAA30333)
Full Name
NMDAR1 Antibody
Gene Names
GRIN1; NR1; MRD8; GluN1; NMDA1; NMDAR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Moesin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Moesin, Monoclonal Antibody (Cat# AAA30135)
Full Name
Moesin Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
EDG1, Monoclonal Antibody (Cat# AAA30309)
Full Name
EDG1 Antibody
Gene Names
S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
ICC (Immunocytochemistry)
(Figure 10 Immunocytochemistry Validation of PD-L1Immunocytochemical analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with at 1 μg/ml, followed by Goat anti-mouse IgG secondary antibody at 1/250 dilution (red). Lower left: Use mouse IgG antibody at 1 μg/ml as control.)
PDL1, Monoclonal Antibody (Cat# AAA10992)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of C6 cells using anti-non-muscle Myosin IIB/MYH10 antibody (AAA19262).Overlay histogram showing C6 cells stained with AAA19262 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-non-muscle Myosin IIB/MYH10 Antibody (AAA19262,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
non-muscle Myosin IIB/MYH10, Polyclonal Antibody (Cat# AAA19262)
Full Name
Anti-non-muscle Myosin IIB/MYH10 Antibody
Gene Names
MYH10; NMMHCB; NMMHC-IIB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
KAT3B/p300, Polyclonal Antibody (Cat# AAA31393)
Full Name
Phospho-KAT3B/p300 (Ser1834) Antibody
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using TXN2 antibody. Blue: DAPI for nuclear staining.)
TXN2, Polyclonal Antibody (Cat# AAA28253)
Full Name
TXN2 Polyclonal Antibody
Gene Names
TXN2; TXN; MTRX; TRX2; MT-TRX; COXPD29
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TOP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Topoisomerase I, Monoclonal Antibody (Cat# AAA30133)
Full Name
Topoisomerase I Antibody
Gene Names
TOP1; TOPI
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with NF-kB p65 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NF-kB, Monoclonal Antibody (Cat# AAA30004)
Full Name
NF-kB p65 Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(AAA31092 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT3, Polyclonal Antibody (Cat# AAA31092)
Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody at dilution of 1:100 (40x lens).)
HDAC2, Polyclonal Antibody (Cat# AAA10734)
Full Name
HDAC2 Polyclonal Antibody
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using DDX5 antibody. Blue: DAPI for nuclear staining.)
DDX5, Polyclonal Antibody (Cat# AAA28263)
Full Name
DDX5 Polyclonal Antibody
Gene Names
DDX5; p68; HLR1; G17P1; HUMP68
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
Prohibitin Mitochondrial Marker, Polyclonal Antibody (Cat# AAA29870)
Full Name
Prohibitin Mitochondrial Marker Antibody
Gene Names
PHB; PHB1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Cathepsin LVKH, Monoclonal Antibody (Cat# AAA30319)
Full Name
Cathepsin LVKH Antibody
Gene Names
CTSL1; MEP; CATL; CTSL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with PARK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
PARK7/DJ1, Monoclonal Antibody (Cat# AAA30163)
Full Name
PARK7/DJ1 Antibody
Gene Names
PARK7; DJ1; DJ-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(HAS2 Antibody (Center) (AAA28714) flow cytometric analysis of K562 cells (bottom histogram) compared to a Rabbit IgG Isotype control (negative control-top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HAS2, Polyclonal Antibody (Cat# AAA28714)
Full Name
HAS2 Antibody (Center)
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of p70 S6 Kinase expression in mouse muscle tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31088)
Full Name
p70 S6 Kinase Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U-87 cells using anti-ATP citrate lyase antibody (AAA11689).Overlay histogram showing U-87 cells stained with AAA11689 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP citrate lyase Antibody (AAA11689,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP citrate lyase, Polyclonal Antibody (Cat# AAA11689)
Full Name
Anti-ATP citrate lyase Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Tuberin/TSC2, Polyclonal Antibody (Cat# AAA31441)
Full Name
Phospho-Tuberin/TSC2 (Ser1254) Antibody
Gene Names
TSC2; LAM; TSC4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing