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2304 results for "Secondary Antibody" - showing 50-100
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with LDL Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
LDL, Monoclonal Antibody (Cat# AAA30045)
Full Name
LDL Receptor Antibody
Gene Names
LDLR; FH; FHC; LDLCQ2
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis extracts of 293 cell line, using MonoMethyl-Histone H3-K9 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
H3K9me1, Antibody (Cat# AAA10652)
Full Name
Histone H3K9me1 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
Topoisomerase II alpha, Polyclonal Antibody (Cat# AAA29961)
Full Name
Topoisomerase II alpha Antibody
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat skeletal muscle using MAPK3 Antibody at dilution of 1:100 (40x lens).)
MAPK3, Polyclonal Antibody (Cat# AAA10646)
Full Name
MAPK3 Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IP (Immunoprecipitation)
(Figure 11 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10944)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A549 cells with Syntenin antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Syntenin, Monoclonal Antibody (Cat# AAA30525)
Full Name
Syntenin Antibody
Gene Names
SDCBP; ST1; MDA9; SYCL; MDA-9; TACIP18
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)
RYK, Polyclonal Antibody (Cat# AAA28767)
Full Name
RYK Antibody
Gene Names
RYK; JTK5; RYK1; JTK5A; D3S3195
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
GSK3 beta, Polyclonal Antibody (Cat# AAA31297)
Full Name
Phospho-GSK3 beta (Thr390) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A/C antibody. Western blot was performed from the immunoprecipitate using Lamin A/C antibody at a dilition of 1:20000.)
Lamin A/C, Monoclonal Antibody (Cat# AAA28306)
Full Name
[KO Validated] Lamin A/C Rabbit mAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
BRCA1, Polyclonal Antibody (Cat# AAA31415)
Full Name
Phospho-BRCA1 (Ser1497) Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (80%), Dog (80%)
Predicted Reactivity: Bovine (80%), Dog (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using EDA Antibody at dilution of 1:100 (40x lens).)
EDA, Polyclonal Antibody (Cat# AAA10715)
Full Name
EDA Polyclonal Antibody
Gene Names
EDA; ED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; STHAGX1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26902)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26762)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (HRP)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
WB (Western Blot)
(Western blot analysis of HDAC5 expression in mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
HDAC5, Polyclonal Antibody (Cat# AAA31097)
Full Name
HDAC5 Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA30931 staining NIH/3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Acetyl-p53, Polyclonal Antibody (Cat# AAA30931)
Full Name
Acetyl-p53 (Lys317) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Pumilio 1, Monoclonal Antibody (Cat# AAA30481)
Full Name
Pumilio 1 Antibody
Gene Names
PUM1; PUMH; HSPUM; PUMH1; PUML1; SCA47
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
ELISA
(Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9083 does not distinguish between WT and Delta.)
COVID 19 Delta Variant Spike S1 (His-Avi Tag) Coronavirus, Recombinant Protein (Cat# AAA11040)
Full Name
SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein
Applications
Western Blot
Purity
> 90% as determined by Bis-Tris PAGE
Pricing
IHC (Immunohistochemistry)
(RNASEK Antibody (Center) (AAA28700) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RNASEK Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
RNASEK, Polyclonal Antibody (Cat# AAA28700)
Full Name
RNASEK Antibody (Center)
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using MAPK8 antibody at dilution of 1:100 (40x lens).)
MAPK8, Polyclonal Antibody (Cat# AAA10635)
Full Name
MAPK8 Polyclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
AIF, Monoclonal Antibody (Cat# AAA30011)
Full Name
AIF Antibody
Gene Names
AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31356)
Full Name
Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody. Western blot was performed from the immunoprecipitate using PPP4C antibody at a dilition of 1:1000.)
PPP4C, Polyclonal Antibody (Cat# AAA10744)
Full Name
PPP4C Polyclonal Antibody
Gene Names
PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with p-HSF1 (S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary)
HSF1, Monoclonal Antibody (Cat# AAA29774)
Full Name
HSF1 (Phospho-S326) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ARRB1, Polyclonal Antibody (Cat# AAA31314)
Full Name
Phospho-ARRB1 (Ser412) Antibody
Gene Names
ARRB1; ARB1; ARR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flotillin 1, Monoclonal Antibody (Cat# AAA30470)
Full Name
Flotillin 1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
S100A8, Monoclonal Antibody (Cat# AAA14670)
Full Name
S100A8 (MRP-8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
PD-L1, Monoclonal Antibody (Cat# AAA27017)
Full Name
PD-L1 Monoclonal Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
WB (Western Blot)
(Figure 6 WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Spike RBD antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.)
COVID 19 Delta Variant Spike RBD (His-Avi Tag) Coronavirus, Recombinant Protein (Cat# AAA11039)
Full Name
SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) Recombinant Protein
Applications
Western Blot
Purity
> 95% as determined by Bis-Tris PAGE
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Cyclin H, Monoclonal Antibody (Cat# AAA30178)
Full Name
Cyclin H Antibody
Gene Names
CCNH; CAK; p34; p37
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
PTP1B, Monoclonal Antibody (Cat# AAA30257)
Full Name
PTP1B Antibody
Gene Names
PTPN1; PTP1B
Reactivity
Human, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Mouse spleen, using Cytochrome c Antibody. The lane on the left was treated with blocking peptide.)
Cytochrome c, Polyclonal Antibody (Cat# AAA31144)
Full Name
Cytochrome c Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat, Monkey
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HepG2 and 293, using CALR Antibody.)
CALR, Polyclonal Antibody (Cat# AAA31108)
Full Name
CALR Antibody
Gene Names
CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemistry)
(Immunofluorescent analysis of U251 cells using AAA18715 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Plasminogen activator inhibitor 1, Polyclonal Antibody (Cat# AAA18715)
Full Name
Rabbit anti-human Plasminogen activator inhibitor 1 polyclonal Antibody
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
TNF II, Monoclonal Antibody (Cat# AAA30327)
Full Name
TNF Receptor II Antibody
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
Application Data
(Figure 10 KD Validation in HeLa cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 ?M luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies (1139). DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10952)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using RSRC1 antibody.)
RSRC1, Polyclonal Antibody (Cat# AAA10780)
Full Name
RSRC1 Polyclonal Antibody
Gene Names
RSRC1; BM-011; SFRS21; SRrp53
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
eIF5A, Monoclonal Antibody (Cat# AAA30124)
Full Name
eIF5A Antibody
Gene Names
EIF5A; EIF-5A; EIF5A1; eIF5AI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
KSR2, Polyclonal Antibody (Cat# AAA31343)
Full Name
Phospho-KSR2 (Thr497) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (91%), Xenopus (91%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (91%), Xenopus (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
YAP, Polyclonal Antibody (Cat# AAA31298)
Full Name
Phospho-YAP (Ser941) Antibody
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31089 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
AKT, Polyclonal Antibody (Cat# AAA31089)
Full Name
AKT Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NCF4 antibody.Blue: DAPI for nuclear staining.)
NCF4, Polyclonal Antibody (Cat# AAA10738)
Full Name
NCF4 Polyclonal Antibody
Gene Names
NCF4; NCF; P40PHOX; SH3PXD4
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A431 cells with PAK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
PAK2, Monoclonal Antibody (Cat# AAA30469)
Full Name
PAK2 Antibody
Gene Names
PAK2; PAK65; PAKgamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using TP53 antibody.)
TP53, Polyclonal Antibody (Cat# AAA28237)
Full Name
TP53 Polyclonal Antibody
Gene Names
Trp53; bbl; bfy; bhy; p44; p53; Tp53
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PPAR alpha, Polyclonal Antibody (Cat# AAA31448)
Full Name
Phospho-PPAR alpha (Ser12) Antibody
Gene Names
PPARA; PPAR; NR1C1; hPPAR; PPARalpha
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (83%)
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (83%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Catenin-gamma expression in mouse tissue extract)
Catenin-gamma, Polyclonal Antibody (Cat# AAA31082)
Full Name
Catenin-gamma Antibody
Gene Names
JUP; DP3; PDGB; PKGB; CTNNG; DPIII
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat brain using HDGF Antibody at dilution of 1:100 (40x lens).)
HDGF, Polyclonal Antibody (Cat# AAA28164)
Full Name
HDGF Polyclonal Antibody
Gene Names
HDGF; HMG1L2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
FOXO4, Polyclonal Antibody (Cat# AAA31400)
Full Name
Phospho-FOXO4 (Thr455) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (91%), Horse (83%), Sheep (91%), Dog (91%)
Predicted Reactivity: Bovine (91%), Horse (83%), Sheep (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
HIST1H2AG, Polyclonal Antibody (Cat# AAA28746)
Full Name
HIST1H2AG Antibody (Center)
Gene Names
HIST1H2AI; H2A/c; H2AFC
Reactivity
Human (Predicted Reactivity: Rat, Mouse, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila)
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Caveolin 2, Polyclonal Antibody (Cat# AAA31397)
Full Name
Phospho-Caveolin 2 (Ser36) Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing